公开(公告)号：WO2016142687A1

公开(公告)日：2016-09-15

申请号：PCT/GB2016/050617

申请日：2016-03-07

Applicant: UNIVERSITY OF SOUTHAMPTON

Inventor： BARTLETT, Philip Nigel ,  BROWN, Tom ,  PAPADOPOULOU, Evanthia ,  GALE, Nittaya

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  C12Q2565/607 ,  C12Q2565/632 ,  C12Q2525/197 ,  C12Q2565/519

Abstract: The present invention relates to a method of analysing nucleic acid in a sample comprising:- providing a nucleic acid probe, which is anchored to a substrate surface only from one or more point(s) located in a mid-region of the nucleic acid probe; and detecting the presence or absence of a target nucleic acid sequence in the sample by hybridisation of the nucleic acid probe with the target nucleic acid sequence if present.

Abstract translation: 本发明涉及一种分析样品中的核酸的方法，包括： - 提供核酸探针，其仅从位于核酸探针的中间区域的一个或多个位点锚定到底物表面 ; 以及通过核酸探针与靶核酸序列（如果存在的话）杂交来检测样品中靶核酸序列的存在或不存在。

公开(公告)号：WO2009053679A1

公开(公告)日：2009-04-30

申请号：PCT/GB2008/003555

申请日：2008-10-21

Applicant: LGC LIMITED ,  UNIVERSITY OF SOUTHAMPTON ,  GALE, Nittaya ,  DEBENHAM, Paul ,  FRENCH, David, John ,  HOWARD, Rebecca ,  MCDOWELL, David, Gordon ,  BROWN, Tom

Inventor： GALE, Nittaya ,  DEBENHAM, Paul ,  FRENCH, David, John ,  HOWARD, Rebecca ,  MCDOWELL, David, Gordon ,  BROWN, Tom

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q2527/107 ,  C12Q2525/186 ,  C12Q2525/151

Abstract: A method for determining the number of tandem repeats in a target polynucleotide, the method comprising (a) providing a sample containing the target polynucleotide, wherein one or more of the tandem repeats in the target polynucleotide is in single stranded form, (b) hybridising a labelled probe oligonucleotide to the single stranded portion of the target polynucleotide, wherein the probe oligonucleotide is complementary to at least one of the tandem repeats, and at least 5 nucleotides of the probe oligonucleotide are complementary to the tandem repeats, in the single stranded portion of the target polynucleotide, and (c) determining the number of tandem repeats in the target polynucleotide based on the hybridisation of the probe oligonucleotide to the single stranded portion of the target polynucleotide.

Abstract translation: 一种用于确定靶多核苷酸中的串联重复数目的方法，所述方法包括（a）提供含有靶多核苷酸的样品，其中靶多核苷酸中的一个或多个串联重复单位为单链形式，（b）杂交 对靶多核苷酸的单链部分的标记探针寡核苷酸，其中所述探针寡核苷酸与至少一个串联重复序列互补，并且探针寡核苷酸的至少5个核苷酸与串联重复序列互补，在单链部分 和（c）基于探针寡核苷酸与靶多核苷酸的单链部分的杂交来确定靶多核苷酸中串联重复序列的数目。

公开(公告)号：WO2008120016A1

公开(公告)日：2008-10-09

申请号：PCT/GB2008/050228

申请日：2008-03-31

Applicant: UNIVERSITY OF SOUTHAMPTON ,  BROWN, Tom ,  KUMAR, Ravindra ,  EL-SAGHEER, Afaf H.

Inventor： BROWN, Tom ,  KUMAR, Ravindra ,  EL-SAGHEER, Afaf H.

IPC: C07H21/00 ,  B01J19/00

CPC classification number: C07H21/00

Abstract: Use of azide/alkyne coupling (click ligation) for oligonucleotide circularisation is proposed for both therapeutic and nanotechnology applications. Such non-templated single-strand circularisation may be the first step in building a more complex catenane by further templated-directed click ligation of olignucleotide sequences, e.g. a double- stranded cyclic oligonucleotide or pseudo-hexagon. Circularisation using click ligation may be used to improve stability of therapeutic oligonucleotides to enzyme degradation in vivo.

Abstract translation: 提出了用于寡核苷酸环化的叠氮化物/炔炔偶联（点击连接）用于治疗和纳米技术应用。 这种非模板化的单链环化可能是通过进一步模板化定向的双核苷酸序列的结合来构建更复杂的正链中的第一步。 双链环寡核苷酸或伪六边形。 使用点击连接的循环可以用于提高治疗性寡核苷酸在体内酶降解的稳定性。

公开(公告)号：WO2014202938A3

公开(公告)日：2014-12-24

申请号：PCT/GB2014/000240

申请日：2014-06-18

Applicant: LGC LIMITED ,  UNIVERSITY OF SOUTHAMPTON

Inventor： FRENCH, David ,  DEBENHAM, Paul ,  HOWARD, Rebecca ,  BROWN, Tom ,  RICHARDSON, James

IPC: C12Q1/68

Abstract: There is provided a method of detecting the presence of a target polynucleotide and/or sequence variations within the target polynucleotide using a probe system comprising two independent partner oligonucleotide components wherein the first oligonucleotide has a first and second section, wherein the first section comprises a nucleotide sequence that is labelled with at least one visually detectable label and is not capable of hybridising to the nucleotide sequence of the target polynucleotide; and wherein the second section comprises a nucleotide sequence that is capable of hybridising to a portion of the sequence of the target polynucleotide; and the second oligonucleotide has a first and second section, wherein the first section comprises a nucleotide sequence which is capable of hybridising to a nucleotide sequence of the first section of the first oligonucleotide; and the second section comprises a nucleotide sequence that is capable of hybridising to a nucleotide sequence of the target polynucleotide that is adjacent to the nucleotide sequence that the second section of the first oligonucleotide is capable of hybridising to. There are also provided alternative methods using additional oligonucleotides and probes for use in such methods.

公开(公告)号：WO2015177520A1

公开(公告)日：2015-11-26

申请号：PCT/GB2015/051451

申请日：2015-05-18

Applicant: UNIVERSITY OF SOUTHAMPTON

Inventor： ELSAGHEER, Afaf H. ,  BROWN, Tom

IPC: C12Q1/68

CPC classification number: C12Q1/6855 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q2521/107 ,  C12Q2523/109 ,  C12Q2525/121 ,  C12Q2525/191

Abstract: The invention relates to a method of nucleic acid processing comprising: providing an adapted nucleic acid fragment having a triazole linkage therein; and transcribing the adapted nucleic acid fragment with reverse transcriptase. The invention further relates to kits and uses associated with the method.

Abstract translation: 本发明涉及核酸加工方法，其包括：提供其中具有三唑键的适配核酸片段; 并用逆转录酶转录适应的核酸片段。 本发明还涉及与该方法相关联的试剂盒和用途。

公开(公告)号：WO2009106834A1

公开(公告)日：2009-09-03

申请号：PCT/GB2009/000541

申请日：2009-02-27

Applicant: UNIVERSITY OF SOUTHAMPTON ,  BARTLETT, Philip, Niger ,  BROWN, Tom ,  RICHARDSON, James, Alistair ,  MAHAJAN, Sumeet

Inventor： BARTLETT, Philip, Niger ,  BROWN, Tom ,  RICHARDSON, James, Alistair ,  MAHAJAN, Sumeet

IPC: C12Q1/68

CPC classification number: C12Q1/6825 ,  C12Q2565/632 ,  C12Q2565/607 ,  C12Q2527/107

Abstract: The present invention relates, in one aspect, to a method for monitoring the dehybridisation of double stranded nucleic acid, comprising the steps of: (a) providing a nucleic acid probe and a target nucleic acid; (b) forming a double stranded nucleic acid of which at least one of the strands of the nucleic acid is in contact with a solid substrate; (c) applying a potential ramp to the solid substrate; and (d) monitoring the dehybridisation of the double stranded nucleic acid across the potential ramp, wherein the nucleic probe is a linear nucleic acid probe and/or the target nucleic acid comprises a label.

Abstract translation: 本发明一方面涉及用于监测双链核酸脱杂交的方法，包括以下步骤：（a）提供核酸探针和靶核酸; （b）形成双链核酸，其中至少一条核酸链与固体基质接触; （c）向固体基底施加电位斜坡; 和（d）监测双链核酸跨越潜在斜坡的脱杂交，其中所述核酸探针是线性核酸探针和/或靶核酸包含标记。

公开(公告)号：WO2006111760A1

公开(公告)日：2006-10-26

申请号：PCT/GB2006/001457

申请日：2006-04-21

Applicant: UNIVERSITY OF SOUTHAMPTON ,  BOURDAKOS, Konstantinos, Nikolaos ,  YIN, HuaBing ,  SMITH, David, Christopher ,  BROWN, Tom ,  MELVIN, Tracy

Inventor： BOURDAKOS, Konstantinos, Nikolaos ,  YIN, HuaBing ,  SMITH, David, Christopher ,  BROWN, Tom ,  MELVIN, Tracy

IPC: C01B31/08

CPC classification number: B82Y30/00 ,  B82Y40/00 ,  C01B32/17 ,  C01B32/18 ,  C01B2202/02 ,  C01B2202/36

Abstract: A method of purifying and fabricating nanostructures comprises adding to material containing nanostructures of various sizes DNA molecules of a first type that associate only with nanostructures of a certain diameter. The DNA molecules select these nanostructures by forming assemblies with them. The material is then applied to a surface patterned with further DNA molecules that associate with the assemblies, so that the assemblies are immobilised on the surface. AU other material is washed from the surface, leaving only the immobilised assemblies. The nanostructures can then be recovered by dissociating the assemblies from the surface and then disassembling the assemblies to free the nanostructures. Thus nanostructures of one diameter only are separated from the bulk of the material. The immobilising stage can also be used to create desired distributions of same-sized nanostructures on an appropriately patterned surface.

Abstract translation: 一种纯化和制造纳米结构的方法包括向仅含有一定直径的纳米结构的第一类型的DNA分子添加含有各种尺寸的DNA分子的材料。 DNA分子通过与它们形成组件来选择这些纳米结构。 然后将该材料施加到用与组件相关联的另外的DNA分子图案化的表面，使得组件固定在表面上。 所有其他材料从表面洗涤，仅留下固定的组件。 然后可以通过将组件从表面解离并且然后拆卸组件以释放纳米结构来回收纳米结构。 因此，一个直径的纳米结构仅与材料的主体分离。 固定阶段还可以用于在适当图案化的表面上产生相同尺寸的纳米结构的期望分布。