摘要:
The present invention is directed to a method of modifying feather raw material comprising the steps of: (a) treating feather raw material in a first aqueous solution containing sulphite ions, wherein the treatment is carried out at pH 6 –8, reaching temperature of about 90 degrees Celsius, the treatment lasting at least 30minutes; (b) separating the sulfonated feather material obtained from step (a);(c) treating the sulfonated feather material in a second aqueous solution, wherein the treatment is carried out at pH 1.5 –3.0 and at temperature of 10 –30 degrees Celsius, the treatment lasting at least 5 minutes;and (d) adjusting the pH of the second aqueous solution or the feather material obtained from the second aqueous solution to at least pH4.5 –5.5.The produced protein-rich modified feather product is suitable as an additive for use, e.g., infood industry, animal feeds and cosmetics.
摘要:
The present invention relates to recombinant proteins/peptides from plant and animal materials, compositions comprising the proteins/peptides and methods for making them.
摘要:
The present invention provides a manufacturing method of a hydrolysate of animal protein, comprising: primarily removing fat from a source of the animal protein; and hydrolyzing a resultant product with a protease under a high pressure. In the case of manufacturing a hydrolysate of animal protein by the manufacturing method according to the present invention, a degree of hydrolysis is very high, and since rancidification or growth of microorganisms is suppressed during the manufacturing process, reliability of product quality and energy cost are excellent in economic terms. Further, in a hydrolysate of animal protein according to the present invention, most of the hydrolyzed protein is degraded into 1 kDa or less, and, thus, it is excellent in rate of absorption from the small intestine. Furthermore, the hydrolysate of animal protein according to the present invention is very low in fat, and, thus, even if it is stored for a long time, rancidification or growth of microorganisms is suppressed and denaturation of the product does not occur.
摘要:
A method and system for obtaining samples for proteomic analysis that utilizes pressure and a preselected agent to obtain a processing sample in a significantly shorter period of time than prior art methods and which maintains the integrity of the processing sample through the preparatory process. In one embodiment of the invention, a sample and an enzyme are combined and subjected to a pressure, preferably a pressure cycle range that varies between 0 to 35 kpsi, for a period of time of preferably less than 60 seconds. This process results in producing a sample suitable for analysis, which is preferably introduced to another analytical instrument such as a mass spectrometry instrument, or other device.
摘要:
This invention relates to a method of hydrolysis of the peptide bond between R 1 and B in a specific designed amino acid sequence R1BXJZR 3 R 2 , where R 1 represents a polypeptide of interest, R 2 represents a sequence capable of specific binding to another component or molecule or another domain which needs to be cleaved, R 3 represents an optional short peptide sequence, B represents a residue capable of accepting an acyl group, J represents a residue capable of metal ion binding, and X and Z represent amino acid residues, wherein the said method is based on a novel molecular mechanism of peptide bond hydrolysis, occurring in a specific complex of this metal ion with the BXJZ sequence. This method can be used to remove BXJZR 3 R 2 domains in recombinant polypeptides, such as sequences capable of specific binding to another component or molecule to yield pure, unmodified R 1 polypeptides of interest. The intermediate hydrolysis product can be reacted with other compounds to obtain derivatives of polypeptides of interest modified covalently at the C-terminus.
摘要翻译:本发明涉及在特定设计的氨基酸序列R1BXJZR3R2中水解R 1和B之间的肽键的方法, 其中R 1表示目的多肽,R 2代表能够特异性结合另一组分或分子或需要被切割的另一结构域的序列, 3表示任选的短肽序列,B表示能够接受酰基的残基,J表示能够金属离子结合的残基,X和Z表示氨基酸残基,其中所述方法基于 肽键水解的新型分子机制,在该金属离子与BXJZ序列的特定络合物中发生。 该方法可用于除去重组多肽中的BXJZR 3 R 2 N 2结构域,例如能够特异性结合另一种组分或分子的序列,以产生纯的未修饰的R 1个感兴趣的多肽。 中间水解产物可以与其它化合物反应,以获得在C末端共价修饰的感兴趣的多肽的衍生物。
摘要:
The present invention relates to an improved process for making a mixture of acetate salts of polypeptides, each of which consisting of glutamic acid, alanine, tyrosine and lysine, for use in the treatment of multiple sclerosis.
摘要:
Provided is a method for characterising a polypeptide or a population of polypeptides, which method comprises: (a) contacting a sample comprising one or more polypeptides with a first cleavage agent to generate polypeptide fragments; (b) isolating one or more polypeptides fragments, each fragment comprising the N-terminus or the C-terminus of the polypeptide from which it was fragmented; (c) identifying the isolated fragments by mass spectrometry; (d) repeating steps (a)-(c) on the sample using a second cleavage agent that cleaves at a different site from the first cleavage agent; and (e) characterising the one or more polypeptides in the sample from the fragments identified in steps (c) and (d).