ペプチド組成物およびその製造方法
    2.
    发明申请
    ペプチド組成物およびその製造方法 审中-公开
    肽组合物及其生产方法

    公开(公告)号:WO2017086303A1

    公开(公告)日:2017-05-26

    申请号:PCT/JP2016/083792

    申请日:2016-11-15

    摘要: 本発明は機能性ペプチドを含有するペプチド組成物を提供することを目的とする。本発明によれば、全ペプチド含有量に対して0.00001~0.2質量%のWY、全ペプチド含有量に対して0.000001~0.1質量%のWL、全ペプチド含有量に対して0.000001~0.1質量%のWVおよび全ペプチド含有量に対して0.000005~0.2質量%のGTWYからなる群から選択される1種または2種以上の成分を含んでなるペプチド組成物が提供される。

    摘要翻译: 本发明的目的是提供含有功能性肽的肽组合物。 根据本发明,基于总肽含量为0.00001-0.2重量%的WY,基于总肽含量为0.000001-0.1重量%的WL, 基于总肽含量计,选自0.000001至0.1重量%的WV和0.000005至0.2重量%的GTWY的一种或多种组分 提供肽组合物。

    HYDROLYSATE OF ANIMAL PROTEIN, MANUFACTURING METHOD THEREOF AND USE THEREOF
    4.
    发明申请
    HYDROLYSATE OF ANIMAL PROTEIN, MANUFACTURING METHOD THEREOF AND USE THEREOF 审中-公开
    动物蛋白的水解产物及其制备方法及其用途

    公开(公告)号:WO2015050294A1

    公开(公告)日:2015-04-09

    申请号:PCT/KR2014/001059

    申请日:2014-02-07

    申请人: INNOWAY CO., LTD

    IPC分类号: C12P21/06 C07K1/12 A23J3/30

    摘要: The present invention provides a manufacturing method of a hydrolysate of animal protein, comprising: primarily removing fat from a source of the animal protein; and hydrolyzing a resultant product with a protease under a high pressure. In the case of manufacturing a hydrolysate of animal protein by the manufacturing method according to the present invention, a degree of hydrolysis is very high, and since rancidification or growth of microorganisms is suppressed during the manufacturing process, reliability of product quality and energy cost are excellent in economic terms. Further, in a hydrolysate of animal protein according to the present invention, most of the hydrolyzed protein is degraded into 1 kDa or less, and, thus, it is excellent in rate of absorption from the small intestine. Furthermore, the hydrolysate of animal protein according to the present invention is very low in fat, and, thus, even if it is stored for a long time, rancidification or growth of microorganisms is suppressed and denaturation of the product does not occur.

    摘要翻译: 本发明提供了动物蛋白质水解产物的制造方法,其特征在于:主要从动物蛋白质来源除去脂肪; 并在高压下用蛋白酶水解所得产物。 在通过本发明的制造方法制造动物蛋白质水解物的情况下,水解度非常高,并且在制造过程中抑制微生物的侵蚀或生长,因此产品质量和能量成本的可靠性是 经济条件好。 此外,在本发明的动物蛋白质的水解物中,大部分水解蛋白质降解为1kDa以下,因此从小肠吸收率优异。 此外,根据本发明的动物蛋白质的水解产物的脂肪含量非常低,因此即使长时间储存​​,抑制微生物的酸化或生长,也不会产生变性。

    タンパク質合成促進剤
    5.
    发明申请
    タンパク質合成促進剤 审中-公开
    蛋白质合成促进剂

    公开(公告)号:WO2011099548A1

    公开(公告)日:2011-08-18

    申请号:PCT/JP2011/052841

    申请日:2011-02-10

    发明人: 三浦 晋

    摘要:  タンパク質の合成促進効果を有するタンパク質合成促進剤の提供。 分子量分布が10kDa以下でメインピーク200Da~3kDa、APL(平均ペプチド鎖長)が2~8、全ての構成成分に対する遊離アミノ酸含量が20%以下、分岐鎖アミノ酸含量が20%以上、抗原性はβ-ラクトグロブリンの抗原性の1/100,000以下であるホエータンパク質加水分解物を用いたタンパク質合成促進剤を提供する。

    摘要翻译: 提供了具有促进蛋白质合成的作用的蛋白质合成促进剂。 蛋白质合成促进剂使用具有10kDa或更低分子量分布的乳清蛋白水解产物,主峰在200Da至3kDa范围内,平均肽链长度(APL)为2至8,游离氨基酸 含量为20%以下,支链氨基酸含量相对于所有构成成分为20%以上,与β-乳球蛋白的抗原性相比,抗原性为1 / 100,000以下。

    HIGH PRESSURE ENZYMATIC DIGESTION SYSTEM FOR PROTEIN CHARACTERIZATION
    6.
    发明申请
    HIGH PRESSURE ENZYMATIC DIGESTION SYSTEM FOR PROTEIN CHARACTERIZATION 审中-公开
    用于蛋白质表征的高压酶消化系统

    公开(公告)号:WO2009099685A2

    公开(公告)日:2009-08-13

    申请号:PCT/US2009030135

    申请日:2009-01-05

    IPC分类号: C07K1/36 C12N13/00 G01N33/48

    摘要: A method and system for obtaining samples for proteomic analysis that utilizes pressure and a preselected agent to obtain a processing sample in a significantly shorter period of time than prior art methods and which maintains the integrity of the processing sample through the preparatory process. In one embodiment of the invention, a sample and an enzyme are combined and subjected to a pressure, preferably a pressure cycle range that varies between 0 to 35 kpsi, for a period of time of preferably less than 60 seconds. This process results in producing a sample suitable for analysis, which is preferably introduced to another analytical instrument such as a mass spectrometry instrument, or other device.

    摘要翻译: 用于获得用于蛋白质组分析的样品的方法和系统利用压力和预选药剂以比现有技术方法显着更短的时间获得处理样品,并且通过准备过程保持处理样品的完整性。 在本发明的一个实施方案中,将样品和酶结合并且经历优选在0至35kpsi之间变化的压力循环范围的压力持续优选小于60秒的时间段。 该过程导致产生适于分析的样品,其优选引入另一分析仪器如质谱仪或其他装置。

    METHOD OF HYDROLYSIS OF PEPTIDE BOND
    7.
    发明申请
    METHOD OF HYDROLYSIS OF PEPTIDE BOND 审中-公开
    肽结合物的水解方法

    公开(公告)号:WO2007091907A1

    公开(公告)日:2007-08-16

    申请号:PCT/PL2006/000026

    申请日:2006-05-04

    IPC分类号: C07K1/12 C07K19/00

    CPC分类号: C07K1/12

    摘要: This invention relates to a method of hydrolysis of the peptide bond between R 1 and B in a specific designed amino acid sequence R1BXJZR 3 R 2 , where R 1 represents a polypeptide of interest, R 2 represents a sequence capable of specific binding to another component or molecule or another domain which needs to be cleaved, R 3 represents an optional short peptide sequence, B represents a residue capable of accepting an acyl group, J represents a residue capable of metal ion binding, and X and Z represent amino acid residues, wherein the said method is based on a novel molecular mechanism of peptide bond hydrolysis, occurring in a specific complex of this metal ion with the BXJZ sequence. This method can be used to remove BXJZR 3 R 2 domains in recombinant polypeptides, such as sequences capable of specific binding to another component or molecule to yield pure, unmodified R 1 polypeptides of interest. The intermediate hydrolysis product can be reacted with other compounds to obtain derivatives of polypeptides of interest modified covalently at the C-terminus.

    摘要翻译: 本发明涉及在特定设计的氨基酸序列R1BXJZR3R2中水解R 1和B之间的肽键的方法, 其中R 1表示目的多肽,R 2代表能够特异性结合另一组分或分子或需要被切割的另一结构域的序列, 3表示任选的短肽序列,B表示能够接受酰基的残基,J表示能够金属离子结合的残基,X和Z表示氨基酸残基,其中所述方法基于 肽键水解的新型分子机制,在该金属离子与BXJZ序列的特定络合物中发生。 该方法可用于除去重组多肽中的BXJZR 3 R 2 N 2结构域,例如能够特异性结合另一种组分或分子的序列,以产生纯的未修饰的R 1个感兴趣的多肽。 中间水解产物可以与其它化合物反应,以获得在C末端共价修饰的感兴趣的多肽的衍生物。

    CHARACTERISING POLYPEPTIDES THROUGH CLEAVAGE AND MASS SPECTROMETRY
    10.
    发明申请
    CHARACTERISING POLYPEPTIDES THROUGH CLEAVAGE AND MASS SPECTROMETRY 审中-公开
    通过清除和质谱分析表征多聚体

    公开(公告)号:WO00020870A1

    公开(公告)日:2000-04-13

    申请号:PCT/GB1999/003258

    申请日:1999-10-01

    摘要: Provided is a method for characterising a polypeptide or a population of polypeptides, which method comprises: (a) contacting a sample comprising one or more polypeptides with a first cleavage agent to generate polypeptide fragments; (b) isolating one or more polypeptides fragments, each fragment comprising the N-terminus or the C-terminus of the polypeptide from which it was fragmented; (c) identifying the isolated fragments by mass spectrometry; (d) repeating steps (a)-(c) on the sample using a second cleavage agent that cleaves at a different site from the first cleavage agent; and (e) characterising the one or more polypeptides in the sample from the fragments identified in steps (c) and (d).

    摘要翻译: 提供了表征多肽或多肽群体的方法,该方法包括:(a)使包含一种或多种多肽的样品与第一切割剂接触以产生多肽片段; (b)分离一个或多个多肽片段,每个片段包含多肽片段的N末端或C末端; (c)通过质谱鉴定分离的片段; (d)使用在与所述第一切割剂不同的位点切割的第二切割剂对所述样品重复步骤(a) - (c) 和(e)从步骤(c)和(d)中鉴定的片段表征样品中的一种或多种多肽。