公开(公告)号：WO2019117728A1

公开(公告)日：2019-06-20

申请号：PCT/NO2018/050308

申请日：2018-12-13

申请人： ACD PHARMACEUTICALS AS

发明人： JIMÉNEZ MARTÍNEZ, Juan José

IPC分类号： A01K67/027 ,  C12N15/113 ,  C12N15/89 ,  C12N15/88

CPC分类号： A01K67/027 ,  A01K2207/05 ,  A01K2227/40 ,  A01K2267/02 ,  C12N15/113 ,  C12N15/88 ,  C12N15/89 ,  C12N2310/315 ,  C12N2310/3231 ,  C12N2310/341

摘要： This invention relates to methods and compounds for the production of sterile fish and other egg-producing aquatic animals. The methods include/the compounds are useful to cause disruption of gonadal development in fish or other egg-producing aquatic animals through the administration of compounds that lead to the failure of fertile gonadal development, and thus to reproductively sterile fish or other egg-producing aquatic animals. The methods and compounds are for use in e.g. aquaculture, the aquarium trade or control of invasive species.

公开(公告)号：WO2015143930A1

公开(公告)日：2015-10-01

申请号：PCT/CN2015/070017

申请日：2015-01-04

申请人： 上海海洋大学

发明人： 邹曙明 ,  蒋霞云 ,  徐海丽 ,  侯飞 ,  程洛单 ,  沈晓丹 ,  邓寿长

IPC分类号： C12N15/70 ,  C12N9/12 ,  C12N1/21 ,  C12N15/85

CPC分类号： C12N15/89 ,  A01K2227/40 ,  C12N1/20 ,  C12N9/1096 ,  C12N9/1241 ,  C12N15/8509 ,  C12N2800/90 ,  C12Y207/07

摘要： 一种有活性的金鱼gfTP1转座酶蛋白的表达方法，其包括构建包含金鱼Tgf2转座子的gfTP1转座酶读码框的表达载体，转入大肠杆菌Rosetta 1(DE3)后，培养表达菌株至菌液在OD600下的吸光度达到0.3-0.4，加入IPTG，在21-22℃下150-200rpm震荡培养，诱导表达出可溶性的重组蛋白，并纯化获得了有功能活性的转座酶。还提供了金鱼gfTP1转座酶蛋白的表达质粒和表达菌株以及所述菌株在转基因中的应用。

公开(公告)号：WO2013071898A3

公开(公告)日：2013-07-11

申请号：PCT/CN2013070289

申请日：2013-01-10

申请人： HUNAN CHANGSHA AO FIGURE BIOLOG TECHNOLOGY CO LTD ,  PENG JIANJUN

发明人： PENG JIANJUN

IPC分类号： C12M1/00

CPC分类号： C12N15/89 ,  C12M33/04 ,  C12M35/00

摘要： A microinjector needle mounting module and a needle holder assembly, comprising a micro-injection needle (1), a needle seat (2) and a needle holder (3); the microinjector mounting module is formed by means of inserting the rear end of the microinjector (1) into a hole in the base (21) of the needle seat; the needle holder (3) is provided with an inner hole (32) passing through the barrel body; a conical socket (24) is provided inside the needle seat (2) and one end of the needle-holder (3) is provided with a conical connector (31), said conical connector (31) and conical socket (24) thus constituting a mutually-complementary insertion connection. In addition to a micro-injection needle being assembled with the needle seat to form a mounting module, other micro-operation needles such as holding pipettes or biopsy needles can also be assembled into a mounting module with said needle seat.

摘要翻译： 一种微型注射针安装模块和针保持器组件，包括微注射针（1），针座（2）和针保持器（3）; 微型注射器安装模块通过将微型注射器（1）的后端插入针座的基座（21）中的孔中而形成; 针保持器（3）设置有穿过筒体的内孔（32）; 在针座（2）的内部设有锥形插座（24），针架（3）的一端设置有锥形连接器（31），所述锥形连接器（31）和锥形插座（24）构成 互补的插入连接。 除了与针座组装以形成安装模块的微型注射针之外，还可以将其它微操作针（例如保持移液管或活检针）组装到具有所述针座的安装模块中。

公开(公告)号：WO2013075131A1

公开(公告)日：2013-05-23

申请号：PCT/US2012/065930

申请日：2012-11-19

申请人： BRIGHAM YOUNG UNIVERSITY

发明人： ATEN, Quentin T. ,  DAVID, Regis A. ,  BURNETT, Sandra H. ,  JENSEN, Brian D. ,  HOWELL, Larry L.

IPC分类号： C12N15/89 ,  C12N15/87 ,  C12N13/00

CPC分类号： C12N15/8775 ,  C12N13/00 ,  C12N15/89

摘要： Systems, devices, and methods for delivering a biological material into an organelle of a cell are provided. In one aspect, for example, a method for introducing biological material into an organelle of a cell can include bringing into proximity outside of a cell a lance and a preselected biological material, charging the lance with a polarity and a charge sufficient to electrically associate the preselected biological material with a tip portion of the lance, and penetrating an outer portion of the cell with the lance and directing and inserting the lance into the cell but outside of the organelle. The method can further include discharging the lance to release at least a portion of the biological material, charging the lance with an opposite polarity and charge sufficient to electrophoretically drive at least a portion of the biological material into the organelle, and withdrawing the lance from the cell.

摘要翻译： 提供了将生物材料递送到细胞的细胞器中的系统，装置和方法。 在一个方面，例如，将生物材料引入细胞的细胞器的方法可以包括使细胞附着在喷枪和预选的生物材料的外部，用足够的极性和电荷对喷枪进行充电以电连接 预先选择的具有喷枪的尖端部分的生物材料，并且用喷枪穿透细胞的外部部分并且将喷枪引导并插入细胞中，但是在细胞器外部。 该方法还可以包括排出喷枪以释放生物材料的至少一部分，以相反的极性对喷枪进行充电，并充入足以将至少一部分生物材料电泳驱动到细胞器中，并将枪从 细胞。

公开(公告)号：WO2012162546A2

公开(公告)日：2012-11-29

申请号：PCT/US2012/039437

申请日：2012-05-24

申请人： BRIGHAM YOUNG UNIVERSITY ,  ATEN, Quentin T. ,  BURNETT, Sandra H. ,  JENSEN, Brian D. ,  HOWELL, Larry L.

发明人： ATEN, Quentin T. ,  BURNETT, Sandra H. ,  JENSEN, Brian D. ,  HOWELL, Larry L.

IPC分类号： C12M1/00 ,  C12N15/87 ,  C12N15/89 ,  C12M1/42 ,  C12N13/00

CPC分类号： C12N15/89 ,  C12M35/00

摘要： Systems, devices, and methods for delivering a biological material into a cell are provided. In one example, a lance device for introducing biological material into a cell and configured for use in a nanoinjection system including a microscope is provided. Such a device can include a lance having a tip region and a shaft region, wherein the lance is structurally configured to allow entry and movement of the tip region into the cell along an elongate axis of the tip region and along a focal plane of the microscope. In another example, the lance can be configured to allow substantially horizontal entry and movement of the tip region into the cell.

摘要翻译： 提供了将生物材料递送到电池中的系统，装置和方法。 在一个示例中，提供了用于将生物材料引入细胞并配置用于包括显微镜的纳米注射系统中的喷枪装置。 这种装置可以包括具有尖端区域和轴区域的喷枪，其中喷枪在结构上构造成允许尖端区域沿着尖端区域的细长轴线并沿着显微镜的焦平面进入和移动到细胞中 。 在另一示例中，喷枪可以被配置为允许尖端区域基本上水平地进入和移动到电池中。

公开(公告)号：WO2012106536A2

公开(公告)日：2012-08-09

申请号：PCT/US2012/023664

申请日：2012-02-02

申请人： DIEFENBACH, Thomas ,  TUFTS UNIVERSITY

发明人： DIEFENBACH, Thomas

IPC分类号： C12N15/87 ,  C12Q1/68 ,  C12M1/00

CPC分类号： C12N15/87 ,  B01L3/021 ,  C12M33/04 ,  C12M35/04 ,  C12N13/00 ,  C12N15/89

摘要： Systems, tip assemblies, methods and kits are provided for introducing material into cells. The tip assemblies include an attachment portion, a channel portion, and a constriction that function to reduce fluid pressure as a fluid passes through the constriction portion from the channel portion, whereby the tip assemblies form pores in the membranes of cells and introduce material into the cells. The material includes for example one selected from the group of: an inorganic compound, a drug, a genetic material, a protein, a carbohydrate, a synthetic polymer, and a pharmaceutical composition.

摘要翻译： 提供系统，尖端组件，方法和试剂盒，用于将材料引入细胞。 尖端组件包括附接部分，通道部分和收缩部，其作用是当流体从通道部分通过收缩部分时降低流体压力，由此尖端组件在细胞膜中形成孔并将材料引入到 细胞。 该材料包括例如选自以下的一种：无机化合物，药物，遗传物质，蛋白质，碳水化合物，合成聚合物和药物组合物。

公开(公告)号：WO2010118213A2

公开(公告)日：2010-10-14

申请号：PCT/US2010/030364

申请日：2010-04-08

申请人： SURMODICS, INC. ,  MCGONIGLE, Joseph, Schmidt ,  SLAGER, Joram

发明人： MCGONIGLE, Joseph, Schmidt ,  SLAGER, Joram

IPC分类号： A61K9/16

CPC分类号： A61K9/1641 ,  A61K9/0024 ,  A61K9/1647 ,  A61K9/5084 ,  A61K48/0041 ,  A61L17/005 ,  A61L17/12 ,  A61L27/48 ,  A61L27/54 ,  A61L29/126 ,  A61L29/148 ,  A61L31/129 ,  A61L31/16 ,  A61L2300/258 ,  A61L2300/62 ,  C12N15/89 ,  C08L67/04

摘要： Embodiments of the invention include devices and methods for the release of nucleic acid complexes. In an embodiment the invention includes a nucleic acid delivery particle. The delivery particle can include a polymeric matrix including a polyethyleneglycol containing copolymer and a nucleic acid complex disposed within the polymeric matrix. The nucleic acid complex can include a nucleic acid and a carrier agent. In an embodiment the invention includes a medical device including a first polymeric matrix comprising a first polymer and a plurality of nucleic acid delivery particles disposed within the first polymeric matrix. The medical device can be configured to release the nucleic acid complex when the medical device is implanted within a subject. Other embodiments are included herein.

摘要翻译： 本发明的实施方案包括用于释放核酸复合物的装置和方法。 在一个实施方案中，本发明包括核酸递送颗粒。 递送颗粒可以包括聚合物基质，其包含含聚乙二醇的共聚物和设置在聚合物基质内的核酸复合物。 核酸复合物可以包括核酸和载体试剂。 在一个实施方案中，本发明包括医疗装置，其包括第一聚合物基质，其包含第一聚合物和设置在第一聚合物基质内的多个核酸递送颗粒。 医疗装置可被配置为当将医疗装置植入受试者内时释放核酸复合物。 本文还包括其它实施例。

公开(公告)号：WO2009052426A2

公开(公告)日：2009-04-23

申请号：PCT/US2008/080367

申请日：2008-10-17

申请人： THE OHIO STATE UNIVERSITY ,  KAUR, Balveen ,  CHIOCCA, Antonio ,  SAEKI, Yoshinaga

发明人： KAUR, Balveen ,  CHIOCCA, Antonio ,  SAEKI, Yoshinaga

IPC分类号： C12N15/38

CPC分类号： C07K14/4703 ,  A61K35/763 ,  A61K38/00 ,  A61K38/177 ,  A61K48/00 ,  C12N7/00 ,  C12N15/89 ,  C12N2710/16632 ,  C12N2710/16643 ,  C12N2830/60 ,  A61K2300/00

摘要： Malignant tumors that are intrinsically resistant to conventional therapies are significant therapeutic challenges. An embodiment of the present invention provides an oncolytic virus capable of killing target cells, such as a tumor cells. In various embodiments presented herein, the oncolytic virus is armed or encodes a therapeutic polypeptide. In at least one embodiment, a recombinant oncolytic virus has been generated that can specifically replicate in cancer cells leading to their destruction and at the same time secrete robust amounts of an angiostatic factor to inhibit the regrowth of residual disease. Compositions and methods disclosed herein have broad therapeutic applicability.

摘要翻译： 对传统治疗具有内在抗性的恶性肿瘤是重大的治疗挑战。 本发明的一个实施方案提供了能够杀死靶细胞例如肿瘤细胞的溶瘤病毒。 在本文呈现的各种实施方案中，溶瘤病毒携带或编码治疗性多肽。 在至少一个实施方案中，已经产生了重组溶瘤病毒，其可以在癌细胞中特异性复制，导致其破坏并且同时分泌大量的血管抑制因子以抑制残余疾病的再生。 本文公开的组合物和方法具有广泛的治疗适用性。

公开(公告)号：WO2008153968A3

公开(公告)日：2009-02-26

申请号：PCT/US2008007158

申请日：2008-06-06

申请人： UNIV WAKE FOREST HEALTH SCIENCES ,  XU TAO ,  YOO JAMES ,  ATALA ANTHONY

发明人： XU TAO ,  YOO JAMES ,  ATALA ANTHONY

IPC分类号： C12N15/09 ,  C12N15/63

CPC分类号： C12N15/895 ,  A61K31/713 ,  C12N15/63 ,  C12N15/87 ,  C12N15/89

摘要： Provided herein are methods and apparatuses for transfecting a cell with a compound of interest by stressing the cell, e.g. with shear stress. The compound of interest may be nucleic acids, proteins, molecules, nanoparticles, drugs, etc., or any combination thereof. Methods of printing cells with an inkjet printing device are also provided, wherein at least a portion of viable cells (preferably at least 1%) are transfected with a compound of interest. Preferably, at least 25% of the cells are viable after printing. In addition, methods of forming an array of viable cells are provided wherein at least a portion of the viable printed cells (preferably at least 1%) are transfected with at least one compound of interest.

摘要翻译： 本文提供了用于通过施加细胞来转染具有感兴趣的化合物的细胞的方法和装置，例如， 具有剪切应力。 感兴趣的化合物可以是核酸，蛋白质，分子，纳米颗粒，药物等，或其任何组合。 还提供了用喷墨印刷装置印刷细胞的方法，其中至少一部分活细胞（优选至少1％）用感兴趣的化合物转染。 优选地，至少25％的细胞在印刷后是活的。 此外，提供形成活细胞阵列的方法，其中至少一部分活的印刷细胞（优选至少1％）用至少一种目标化合物转染。

公开(公告)号：WO2007018562A2

公开(公告)日：2007-02-15

申请号：PCT/US2005033975

申请日：2005-09-22

申请人： NANOLAB INC

发明人： CAI DONG ,  CARNAHAN DAVID L

IPC分类号： C12N15/09 ,  C12N15/87

CPC分类号： C12N15/89 ,  C12M35/02 ,  C12N15/87 ,  Y10S977/746 ,  Y10S977/748

摘要： A nanostructured molecular delivery vehicle comprising magnetic materials and configured to receive passenger biomolecules. The application of a an appropriate magnetic field having a gradient orients and drives the vehicle into a biological target, which may comprise cells,cell masses, tissue slices, tissues, etc. Under the control of the magnetic field, these vehicles can penetrate cell membranes. Then, the biomolecules carried by the vehicle can be released into the cells to perform their functions. Using this "nanospearing" technique, unprecendented high transfection efficiency has been achieved in several difficult-to-transfect cells. These include, but are not limited to, Bal 17 cells, ex vivo B cells, primary cultured cortical neurons, etc. This method advances the state of the art, providing an improved technique for the introduction of exogenous molecules to cells, with the clinical applications including, but not being limited to, drug delivery, gene therapy, vaccination, etc.

摘要翻译： 纳米结构分子递送载体，其包含磁性材料并且构造成接收乘客生物分子。 施加具有梯度的合适的磁场定向并将车辆驱动到可包括细胞，细胞块，组织切片，组织等的生物靶中。在磁场的控制下，这些载体可以穿透细胞膜 。 然后，车辆携带的生物分子可以释放到细胞中以发挥其功能。 使用这种“纳米级”技术，在几个难以转染的细胞中已经实现了非常重要的高转染效率。 这些方法包括但不限于Bal 17细胞，离体B细胞，原代培养的皮层神经元等。这种方法促进了现有技术的进步，提供了一种用于将外源性分子引入细胞的改进技术，临床 应用包括但不限于药物递送，基因治疗，疫苗接种等