公开(公告)号：WO2019053729A1

公开(公告)日：2019-03-21

申请号：PCT/IL2018/051055

申请日：2018-09-17

Applicant: HADASIT MEDICAL RESEARCH SERVICES & DEVELOPMENT LIMITED

Inventor： ROSENMANN, Hanna

IPC: A61K9/00 ,  A61K47/02 ,  A61K9/08 ,  A61K38/18 ,  A61P31/00 ,  A61K39/395 ,  C07K16/24 ,  A61P25/28

CPC classification number: A61K9/08 ,  A61K9/0019 ,  A61K35/28 ,  A61K35/51 ,  A61K38/1841 ,  A61K38/185 ,  A61K47/02 ,  A61P25/28 ,  C12N5/0619 ,  C12N5/0648 ,  C12N5/0663 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/84 ,  C12N2502/1358

Abstract: The present disclosure provides an enriched artificial cerebrospinal fluid (EA-CSF) composition comprising: an electrolyte containing solution comprising at least one electrolyte selected from the group consisting of sodium ion, potassium ion, calcium ion, magnesium ion, phosphor ion, chloride ion and carbonate ion; and at least one neuroprotective factor. Also provided by the present disclosure are methods for producing the EA-CSF as well as methods of using the same for treating diseases, specifically, neurological diseases.

公开(公告)号：WO2017125917A1

公开(公告)日：2017-07-27

申请号：PCT/IL2017/050058

申请日：2017-01-17

Applicant: AMORPHICAL LTD.

Inventor： BEN, Yosef ,  SHAHAR, Abraham ,  ARAV, Amir

IPC: A61K33/06 ,  A61K33/10 ,  A61K31/66 ,  C12N5/00 ,  C12N5/04 ,  C12N5/073

CPC classification number: C12N5/0018 ,  C12N5/0604 ,  C12N5/0609 ,  C12N5/061 ,  C12N5/0619 ,  C12N5/0658 ,  C12N5/0663 ,  C12N2500/14

Abstract: Stabilized amorphous calcium carbonate (ACC) as a supplement of cell culture media and the cell culture medium supplements comprising stabilized ACC are provided. In particular the stabilized ACC is useful for enhancing the growth of cell and tissue cultures, gametes and embryos in vitro.

Abstract translation: 提供稳定的无定形碳酸钙（ACC）作为细胞培养基的补充物和包含稳定的ACC的细胞培养基补充物。 特别是稳定的ACC可用于增强细胞和组织培养物，配子和胚胎的体外生长。

公开(公告)号：WO2017021831A1

公开(公告)日：2017-02-09

申请号：PCT/IB2016/054519

申请日：2016-07-28

Applicant: BLAST RESEARCH S.R.L.

Inventor： COSTA, Massimo ,  PASSI, Alberto Giuseppe

IPC: C12N5/077 ,  A01N1/02

CPC classification number: C12N5/0656 ,  A01N1/0226 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/34 ,  C12N2500/38 ,  C12N2500/92 ,  C12N2500/95

Abstract: The present invention relates to a cell culture medium, in particular for culturing autologous fibroblasts, for use in aesthetic medicine for skin transplantation, said culture medium being serum-free and being characterized in that it contains glucose in a much lower quantity than conventional culture media.

Abstract translation: 本发明涉及一种细胞培养基，特别是用于培养用于皮肤移植的美容医学的自体成纤维细胞，所述培养基是无血清的，其特征在于，其含有比常规培养基低得多的葡萄糖 。

公开(公告)号：WO2015148943A1

公开(公告)日：2015-10-01

申请号：PCT/US2015/023026

申请日：2015-03-27

Applicant: CONJUGON, INC.

Inventor： WATT, Steven R. ,  DORSEY, Caleb W. ,  SMITH, Joshua A.

IPC: C12N1/00 ,  C12N1/20 ,  C12N1/26

CPC classification number: A61K35/741 ,  A61K9/0034 ,  A61K35/74 ,  A61L29/005 ,  A61L29/085 ,  A61L29/145 ,  A61L2400/10 ,  A61M25/0017 ,  A61M25/0045 ,  A61M2025/0046 ,  C12N1/20 ,  C12N1/32 ,  C12N1/38 ,  C12N2500/05 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/20 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/60 ,  Y02A50/473

Abstract: The invention relates to methods of differentiation, isolation, propagation, and storage of small colony variants (SCVs) of E. coli , preferably E. coli 83972 or E. coli HU2117, or modified or variant forms thereof, and methods for using the prepared SCV bacteria to establish probiotic biofilms in treated subjects and/or on treated medical devices.

Abstract translation: 本发明涉及大肠杆菌，优选大肠杆菌83972或大肠杆菌HU2117的小菌落变异体（SCV）的分化，分离，繁殖和储存方法，或其修饰或变异形式，以及使用制备的 SCV细菌在处理的受试者和/或治疗的医疗装置上建立益生菌生物膜。

公开(公告)号：WO2015055987A1

公开(公告)日：2015-04-23

申请号：PCT/GB2014/053064

申请日：2014-10-13

Applicant: UNIVERSITY COLLEGE CARDIFF CONSULTANTS LIMITED ,  KEMP, Paul ,  ALLEN, Nicholas

Inventor： KEMP, Paul ,  ALLEN, Nicholas

IPC: C12N5/0793

CPC classification number: C12N5/0619 ,  C12N2500/14 ,  C12N2500/38 ,  C12N2501/01 ,  C12N2501/13 ,  C12N2501/40 ,  C12N2501/415 ,  C12N2501/42 ,  C12N2501/845 ,  C12N2501/999 ,  C12N2506/08

Abstract: The invention relates to at least one novel neural cell differentiation medium for the production of neural cells from at least one precursor cell capable of lineage specific differentiation; a method for neural cell differentiation using said medium; the use of said medium and said method for lineage specific differentiation of at least one precursor cell towards a neural cell fate; and a kit of parts comprising said at least one said neural cell differentiation medium.

Abstract translation: 本发明涉及至少一种用于从至少一种能够谱系特异性分化的前体细胞产生神经细胞的新型神经细胞分化培养基; 使用所述培养基的神经细胞分化方法; 所述培养基的使用和所述方法用于至少一种前体细胞对神经细胞命运的谱系特异性分化; 以及包含所述至少一种所述神经细胞分化培养基的试剂盒。

公开(公告)号：WO2015034212A1

公开(公告)日：2015-03-12

申请号：PCT/KR2014/008079

申请日：2014-08-29

Applicant: MEDIPOST CO., LTD.

Inventor： YANG, Yoon-Sun ,  OH, Won Il ,  JIN, Hye Jin ,  KWON, Soon-Jae ,  KIM, Miyeon

IPC: C12N5/0775 ,  C12N5/02 ,  A61K35/12

CPC classification number: C12N5/0665 ,  A61K35/28 ,  C12N2500/02 ,  C12N2500/14 ,  C12N2500/16

Abstract: The present invention relates to a method for culturing mesenchymal stem cells, including isolating mesenchymal stem cells having a size of 8 μm or less and culturing the mesenchymal stem cells in a medium containing calcium and magnesium and under a low oxygen condition. The method of the present invention can significantly improve the proliferative capacity and differentiation potential of the mesenchymal stem cells.

Abstract translation: 本发明涉及培养间充质干细胞的方法，包括分离大小为8μm或更小的间充质干细胞，并在含有钙和镁的培养基中和在低氧条件下培养间充质干细胞。 本发明的方法可以显着改善间充质干细胞的增殖能力和分化潜能。

公开(公告)号：WO2014181954A1

公开(公告)日：2014-11-13

申请号：PCT/KR2014/001013

申请日：2014-02-06

Applicant: 주식회사 케이스템셀

Inventor： 강성근 ,  조정윤 ,  라정찬

IPC: C12N5/02 ,  C12N5/074 ,  C12N5/0775

CPC classification number: C12N5/0667 ,  C12N2500/14 ,  C12N2500/25 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/76 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/40 ,  C12N2501/998 ,  C12N2501/999

Abstract: 본 발명은 줄기세포의 재생능 향상을 위한 아펠린을 함유하는 배지 조성물 및 이를 이용한 줄기세포의 배양방법에 관한 것이다. 본 발명에 따르면, 줄기세포를 세포 특성의 변화 없이 효과적으로 증식 및 배양할 수 있고 활성을 증가시킬 수 있으므로 줄기세포를 이용한 세포치료 효능을 획기적으로 증진시킬 수 있다.

Abstract translation: 本发明涉及含有用于提高干细胞的再生能力的apelin的培养基组合物和使用其的干细胞培养方法。 本发明能够有效地培养和培养干细胞并增加其活性，而不改变细胞特征，从而显着地促进使用干细胞的细胞疗法功效。

公开(公告)号：WO2012129538A1

公开(公告)日：2012-09-27

申请号：PCT/US2012/030446

申请日：2012-03-23

Applicant: WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH ,  BRIGHAM AND WOMEN'S HOSPITAL, INC. ,  INCE, Tan, A.

Inventor： INCE, Tan, A.

IPC: C12N5/071 ,  C12N5/09

CPC classification number: C12N5/0018 ,  C12N5/0682 ,  C12N5/0693 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/20 ,  C12N2500/22 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/40 ,  C12N2500/46 ,  C12N2500/50 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/39 ,  C12N2501/395 ,  G01N33/5008 ,  G01N33/5011

Abstract: Described herein are cell culture media, kits and methods for preparing cell culture media, and methods for culturing cells, for example, cells of the female reproductive tract, and tumor cells.

Abstract translation: 本文描述了用于制备细胞培养基的细胞培养基，试剂盒和方法，以及培养细胞例如雌性生殖道细胞和肿瘤细胞的方法。

公开(公告)号：WO2009151496A2

公开(公告)日：2009-12-17

申请号：PCT/US2009001859

申请日：2009-03-25

Applicant: US GOV HEALTH & HUMAN SERV ,  CONNORS MARK ,  MIGUELES STEPHEN A

Inventor： CONNORS MARK ,  MIGUELES STEPHEN A

IPC: A61K31/235 ,  A61K31/555 ,  A61K33/06 ,  A61P31/18 ,  A61P37/00

CPC classification number: A61K31/235 ,  A61K31/555 ,  A61K35/17 ,  A61K2035/124 ,  C12N5/0636 ,  C12N2500/14 ,  C12N2501/999 ,  A61K2300/00

Abstract: Provided is a method of activating an immune cell of a subject with Human Immunodeficiency Virus (HIV), comprising contacting the immune cell with a phorbol ester and a calcium ionophore. Also provided is a composition comprising immune cells of a subject diagnosed with HIV, wherein the immune cells are activated by contact with a phorbol ester and a calcium ionophore. Methods of using the disclosed compositions are also disclosed.

Abstract translation: 提供了一种用人免疫缺陷病毒（HIV）激活受试者的免疫细胞的方法，包括使免疫细胞与佛波酯和钙离子载体接触。 还提供了包含被诊断为HIV的受试者的免疫细胞的组合物，其中所述免疫细胞通过与佛波酯和钙离子载体接触来活化。 还公开了使用所公开的组合物的方法。

公开(公告)号：WO2007143204A8

公开(公告)日：2008-05-02

申请号：PCT/US2007013178

申请日：2007-06-04

Applicant: SACKSTEIN ROBERT

Inventor： SACKSTEIN ROBERT

IPC: C12N5/00 ,  C12N5/074 ,  C12N5/0775 ,  C12N5/0797 ,  C12P1/00

CPC classification number: C12N9/1051 ,  A61K35/28 ,  A61K35/30 ,  C12N5/0006 ,  C12N5/0623 ,  C12N5/0663 ,  C12N2500/10 ,  C12N2500/14 ,  C12N2500/16 ,  C12N2500/22 ,  C12N2500/34 ,  C12N2501/58 ,  C12N2501/59 ,  C12N2501/70 ,  C12N2506/1369

Abstract: Methods and compositions for modifying glycans (e.g., glycans expressed on the surface of live cells or cell particles) are provided herein.

Abstract translation: 本文提供了用于修饰聚糖（例如，在活细胞或细胞颗粒表面上表达的聚糖）的方法和组合物。