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    MULTI-PURPOSE SUBSTRATES USEFUL FOR CELL CULTURE AND METHODS FOR MAKING AND USING THE SAME
    1.
    发明申请
    MULTI-PURPOSE SUBSTRATES USEFUL FOR CELL CULTURE AND METHODS FOR MAKING AND USING THE SAME 审中-公开
    用于细胞培养的多用途底物及其制备和使用方法

    公开(公告)号：WO2011066101A1

    公开(公告)日：2011-06-03

    申请号：PCT/US2010/056131

    申请日：2010-11-10

    申请人： CORNING INCORPORATED ,  RAO, Hongwei H. ,  TAN, Jian

    发明人： RAO, Hongwei H. ,  TAN, Jian

    IPC分类号： C12N5/00 ,  G01N33/58 ,  C12M1/00

    CPC分类号： C12N5/0068 ,  C12M23/20 ,  C12M25/06 ,  C12N5/0643 ,  C12N2503/00 ,  C12N2533/18 ,  C12N2533/54 ,  G01N33/582

    摘要： Described herein are multi-purpose substrates composed of (1) a base coated with a calcium phosphate coating and (2) a fluorophore-labeled collagen adsorbed on the calcium phosphate coating. The multi-purpose substrates are useful in culturing and studying the activity of a variety of cells. The multi-purpose substrates described herein can be used for both solution- and image -based analysis of cultured cells. New methods for producing and using such coated substrates are also disclosed.

    摘要翻译： 本文描述的是由（1）涂覆有磷酸钙涂层的基底和（2）吸附在磷酸钙涂层上的荧光团标记的胶原构成的多功能底物。 多功能底物可用于培养和研究各种细胞的活性。 本文所述的多用途底物可用于培养细胞的基于溶液和图像的分析。 还公开了用于生产和使用这种涂覆的基材的新方法。

    METHODS OF MODIFYING CD11C+ DENDRITIC CELL DEVELOPMENT TO FORM OSTEOCLASTS FUNCTIONAL IN THE BONE ENVIRONMENT
    2.
    发明申请
    METHODS OF MODIFYING CD11C+ DENDRITIC CELL DEVELOPMENT TO FORM OSTEOCLASTS FUNCTIONAL IN THE BONE ENVIRONMENT 审中-公开
    修改CD11C +细胞发育的方法在骨骼环境中形成骨质疏松功能

    公开(公告)号：WO2006079051A2

    公开(公告)日：2006-07-27

    申请号：PCT/US2006/002397

    申请日：2006-01-24

    申请人： UNIVERSITY OF ROCHESTER ,  TENG, Yen-Tung, A.

    发明人： TENG, Yen-Tung, A.

    IPC分类号： C12N5/08

    CPC分类号： C12N5/0643 ,  A61K2035/124 ,  C12N2501/185 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/998 ,  C12N2502/11

    摘要： An ex vivo method of producing osteoclasts is described that includes providing isolated CD 11 C + dendritic cells and culturing the CD 11 C + dendritic cells in culture medium under conditions effective to produce osteoclasts. Also disclosed are methods of up-regulating or down-regulating bone resorption by manipulating the osteoclastogenesis of CDl Ic + dendritic cells either in vivo or in vitro. Methods of treating an inflammatory bone disease or a metabolic bone disorder in a subject, and screening assays to identify compounds or genes that affect myeloid osteoclastogenesis are also described.

    摘要翻译： 描述了一种离体生产破骨细胞的方法，其包括提供分离的CD 11 C +树突状细胞，并在有效产生的条件下在培养基中培养CD11C +树突状细胞 破骨细胞。 还公开了通过在体内或体外操纵CD11cP +树突状细胞的破骨细胞发生来上调或下调骨吸收的方法。 还描述了治疗受试者的炎症性骨病或代谢性骨病的方法以及用于鉴定影响骨髓破骨细胞发生的化合物或基因的筛选试验。

    METHODS FOR INHIBITING OSTEOCLAST FORMATION OR METHODS FOR INHIBITING BONE RESORPTION
    5.
    发明申请
    METHODS FOR INHIBITING OSTEOCLAST FORMATION OR METHODS FOR INHIBITING BONE RESORPTION 审中-公开
    用于抑制骨质疏松形成的方法或抑制骨骼再分配的方法

    公开(公告)号：WO01025404A1

    公开(公告)日：2001-04-12

    申请号：PCT/JP2000/004146

    申请日：2000-06-23

    IPC分类号： A61K31/59 ,  A61K38/00 ,  A61K38/22 ,  A61K41/00 ,  A61N7/00 ,  A61P19/08 ,  C12N5/078 ,  C12N5/06

    CPC分类号： A61K41/0019 ,  A61N7/00 ,  C12N5/0643

    摘要： A method for inhibiting the formation of osteoclasts or a method for inhibiting bone resorption characterized by effecting UV irradiation in an incubation system containing osteoclast precursor cells and inducers in a liquid medium; or a method for inhibiting the formation of osteoclasts or a method for inhibiting bone resorption characterized by effecting UV irradiation in a co-incubation system containing osteoclast precursor cells and inducers in a liquid medium.

    摘要翻译： 抑制破骨细胞形成的方法或抑制骨吸收的方法，其特征在于在含有破骨细胞前体细胞和诱导剂的液体培养基中的培养体系中进行紫外线照射; 或抑制破骨细胞形成的方法或抑制骨吸收的方法，其特征在于在含有破骨细胞前体细胞和诱导剂的液体培养基中的共孵育体系中进行紫外线照射。

    PREPARATION OF IMMORTALIZED CELLS
    6.
    发明申请
    PREPARATION OF IMMORTALIZED CELLS 审中-公开
    免疫细胞的制备

    公开(公告)号：WO1996007733A1

    公开(公告)日：1996-03-14

    申请号：PCT/US1995011484

    申请日：1995-09-11

    申请人： ZYMOGENETICS, INC. ,  MOORE, Emma, E. ,  MACKAY, Vivian, L.

    发明人： ZYMOGENETICS, INC.

    IPC分类号： C12N05/10

    CPC分类号： C12N5/0654 ,  C12N5/0639 ,  C12N5/0643 ,  C12N2501/392 ,  C12N2503/02 ,  C12N2510/04 ,  C12N2517/02

    摘要： Cell lines have been prepared from growth suppressor gene deficient animals. The cells include immortalized precursor cells and differentiated cells such as osteoclast precursors, osteoblast precursors, megakaryocytes, osteoclasts, osteoblasts, pancreatic alpha -cells, pancreatic beta -cells, pancreatic delta -cells, adipocytes, macrophages, chondrocytes, dendritic cells and hepatocytes. The cells are useful for constructing cDNA and protein libraries, screening agonists and antagonists of compounds and factors that affect metabolic pathways of specific cells and generating cell-specific antibodies.

    摘要翻译： 从生长抑制基因缺陷动物制备细胞系。 细胞包括永生化前体细胞和分化细胞如破骨细胞前体，成骨细胞前体，巨核细胞，破骨细胞，成骨细胞，胰腺α细胞，胰腺β-细胞，胰腺β-细胞，脂肪细胞，巨噬细胞，软骨细胞，树突状细胞和肝细胞。 细胞可用于构建cDNA和蛋白质文库，筛选化合物的激动剂和拮抗剂以及影响特定细胞的代谢途径并产生细胞特异性抗体的因子。

    CELL-BASED METHOD FOR REBUILDING BONE
    7.
    发明申请
    CELL-BASED METHOD FOR REBUILDING BONE 审中-公开
    基于细胞的骨重建方法

    公开(公告)号：WO2010015619A3

    公开(公告)日：2010-04-01

    申请号：PCT/EP2009060078

    申请日：2009-08-04

    申请人： UNIV DRESDEN TECH ,  HOFLACK BERNARD ,  SANCHEZ FERNANDEZ MARIA ARANTZ

    发明人： HOFLACK BERNARD ,  SANCHEZ FERNANDEZ MARIA ARANTZAZU

    IPC分类号： C12N5/071 ,  C12N5/22

    CPC分类号： A61K35/32 ,  A61K9/0019 ,  A61K9/0024 ,  A61K9/06 ,  A61K9/10 ,  A61L27/3821 ,  A61L27/3847 ,  C12N5/0643 ,  C12N2501/185 ,  C12N2501/22

    摘要： The invention relates to the use of in vitro isolated or in vitro differentiated osteoclasts for bone rebuilding, in particular by in vivo application, to trigger bone rebuilding by attracting osteoblasts and preferably also vascular cells to sites of bone defect. It has been found by the inventors that osteoclasts can control bone rebuilding by secreting chemotactic factors and attracting osteoblasts and vascular cells to a site of bone defect. The invention further relates to the use of osteoclasts for the manufacture of a pharmaceutical preparation or medical product containing osteoclasts for the use for bone rebuilding.

    摘要翻译： 本发明涉及体外分离的或体外分化的破骨细胞用于骨重建，特别是通过体内应用，通过吸引成骨细胞并优选将血管细胞诱导到骨缺损部位来引发骨重建。 本发明人已经发现，破骨细胞可通过分泌趋化因子并将成骨细胞和血管细胞吸引到骨缺损部位来控制骨重建。 本发明还涉及破骨细胞用于制造含有用于骨重建的破骨细胞的药物制剂或医药产品的用途。

    CELL-BASED METHOD AND MEANS FOR REBUILDING BONE
    8.
    发明申请
    CELL-BASED METHOD AND MEANS FOR REBUILDING BONE 审中-公开
    基于细胞的方法和手段重建骨

    公开(公告)号：WO2010015619A2

    公开(公告)日：2010-02-11

    申请号：PCT/EP2009/060078

    申请日：2009-08-04

    申请人： TECHNISCHE UNIVERSITÄT DRESDEN ,  HOFLACK, Bernard ,  SANCHEZ FERNANDEZ, Maria, Arantzazu

    发明人： HOFLACK, Bernard ,  SANCHEZ FERNANDEZ, Maria, Arantzazu

    IPC分类号： C12N5/06

    CPC分类号： A61K35/32 ,  A61K9/0019 ,  A61K9/0024 ,  A61K9/06 ,  A61K9/10 ,  A61L27/3821 ,  A61L27/3847 ,  C12N5/0643 ,  C12N2501/185 ,  C12N2501/22

    摘要： The invention relates to the use of in vitro isolated or in vitro differentiated osteoclasts for bone rebuilding, in particular by in vivo application, to trigger bone rebuilding by attracting osteoblasts and preferably also vascular cells to sites of bone defect. It has been found by the inventors that osteoclasts can control bone rebuilding by secreting chemotactic factors and attracting osteoblasts and vascular cells to a site of bone defect. The invention further relates to the use of osteoclasts for the manufacture of a pharmaceutical preparation or medical product containing osteoclasts for the use for bone rebuilding.

    摘要翻译： 本发明涉及体外分离或体外分化的破骨细胞用于骨重建的用途，特别是通过体内应用，通过吸引成骨细胞并优选将血管细胞诱导到骨缺损部位来引发骨重建。 本发明人已经发现，破骨细胞可通过分泌趋化因子并将成骨细胞和血管细胞吸引到骨缺损部位来控制骨重建。 本发明还涉及破骨细胞用于制造含有用于骨重建的破骨细胞的药物制剂或医药产品的用途。

    METHOD OF RESISTING OSTEOCLAST FORMATION
    10.
    发明申请
    METHOD OF RESISTING OSTEOCLAST FORMATION 审中-公开
    电磁波形成的方法

    公开(公告)号：WO2005035576A3

    公开(公告)日：2005-12-15

    申请号：PCT/US2004027796

    申请日：2004-08-27

    申请人： MEDIMMUNE INC ,  UNIV PITTSBURGH ,  WHITE WENDY ,  ROODMAN G DAVID ,  CHOI SUN JIN ,  OBA YASUO

    发明人： WHITE WENDY ,  ROODMAN G DAVID ,  CHOI SUN JIN ,  OBA YASUO

    IPC分类号： C07K14/52 ,  C07K16/24 ,  C12N5/078

    CPC分类号： C07K14/52 ,  C07K16/24 ,  C12N5/0643 ,  C12N2500/38 ,  C12N2501/185 ,  C12N2501/21

    摘要： A method of inhibiting osteoclast formation including inhibiting eosinophil chemotactic factor-L expression or activity. This may be accomplished by a number of means, including the use of anti-ECF-L antibody, antisense S-oligonucleotide to ECF-L, mECF-L polyclonal antisera, rabbit preimmune antisera, OPG RANK-Fc and combinations thereof, as well as other inhibiting materials. In another embodiment, osteoclast formation is inhibited by inhibiting RANKL formation. In a further embodiment, a method of inhibiting osteoclast formation is accomplished by means of mECF-L in the presence of RANKL.

    摘要翻译： 一种抑制破骨细胞形成的方法，包括抑制嗜酸性粒细胞趋化因子-L表达或活性。 这可以通过许多方法来实现，包括使用抗ECF-L抗体，ECF-L的反义S-寡核苷酸，mECF-L多克隆抗血清，兔免疫前抗血清，OPG RANK-Fc及其组合 作为其他抑制材料。 在另一个实施方案中，通过抑制RANKL形成来抑制破骨细胞的形成。 在另一个实施方案中，通过在RANKL存在下的mECF-L来实现抑制破骨细胞形成的方法。