公开(公告)号：WO2008143722A2

公开(公告)日：2008-11-27

申请号：PCT/US2008/001833

申请日：2008-02-12

Applicant: CHARM SCIENCES, INC. ,  SALTER, Robert, S. ,  DURBIN, Gregory, W.

Inventor： SALTER, Robert, S. ,  DURBIN, Gregory, W.

IPC: C12Q1/70

CPC classification number: C12Q1/04 ,  C12Q2334/22 ,  C12Q2334/52

Abstract: Phage can be detected as rapid indicators of the hygienic quality of a sample. Both continuous flow methods and devices and single sample methods and devices, of various volumes, can be used. Test kits can be provided in easy-to-use formats. Certain phage, such as coliphage, are useful as indicators of fecal contamination.

Abstract translation: 可以将噬菌体作为样品卫生质量的快速指标。 可以使用不同体积的连续流动方法和装置以及单个样品方法和装置。 测试套件可以以易于使用的格式提供。 某些噬菌体，如大肠杆菌噬菌体，可用作粪便污染的指标。

公开(公告)号：WO2002071071A2

公开(公告)日：2002-09-12

申请号：PCT/GB2002/000901

申请日：2002-03-05

Applicant: UNIVERSITY OF SUNDERLAND ,  ROWELL, Frederick, John

Inventor： ROWELL, Frederick, John

IPC: G01N33/58

CPC classification number: G01N33/581 ,  C12Q2326/92 ,  C12Q2334/52

Abstract: There is described an assay system wich comprises; (i) a first dip strip comprising a first material at least partially coated with a repoter converter in an inert carrier; and (ii) a second dip strip comprising a second strip material at least partially coated with a reporter precursor. There is also described an assay system comprising a single foldable or frangible strip and a method of quantitative determination of an assay.

Abstract translation: 描述了一种测定系统，其包括： （i）第一浸渍条，其包含在惰性载体中至少部分地涂覆有回转体转化器的第一材料; 和（ii）第二浸渍条，其包含至少部分涂覆有报告基因前体的第二条带材料。 还描述了包含单个可折叠或易碎条的测定系统和定量测定方法。

公开(公告)号：WO02053705A1

公开(公告)日：2002-07-11

申请号：PCT/FR2002/000024

申请日：2002-01-04

IPC: G01N21/77 ,  C12N1/20 ,  C12Q1/04 ,  G01N21/78

CPC classification number: C12Q1/04 ,  C12N1/20 ,  C12Q2334/22 ,  C12Q2334/50 ,  C12Q2334/52

Abstract: The invention concerns a culture medium for isolating bacteria of Listeria genus, in particular L. monocytogenes , characterised in that it comprises in a Listeria culture medium, at least a specific agent for identifying gamma -mannosidase for use in solid medium.

Abstract translation: 本发明涉及用于分离利斯特氏菌属细菌的培养基，特别是单核细胞增生李斯特氏菌，其特征在于其在利斯特氏菌培养基中包含用于鉴定用于固体培养基的γ-甘露糖苷酶的至少一种特异性试剂。

公开(公告)号：WO1997042342A1

公开(公告)日：1997-11-13

申请号：PCT/US1997007658

申请日：1997-05-06

Applicant: KIRKEGAARD & PERRY LABORATORIES, INC.

Inventor： KIRKEGAARD & PERRY LABORATORIES, INC. ,  COBBS, Carrington, S. ,  WOERNER, Thomas, M.

IPC: C12Q01/44

CPC classification number: G01N33/581 ,  C12Q1/42 ,  C12Q1/6897 ,  C12Q2326/92 ,  C12Q2334/52 ,  Y10S435/962 ,  Y10S435/963 ,  Y10S435/975 ,  Y10S436/808

Abstract: This invention relates to an improved method for detecting and quantifying the presence of a target molecule, such as an antigen, an antibody or a polynucleotide, in a sample which method uses alkaline phosphatase as the reporter enzyme and the reduction of a tetrazolium salt to a formazan as part of the detection/signaling system.

Abstract translation: 本发明涉及一种用于检测和定量目标分子如抗原，抗体或多核苷酸的存在的改进方法，该方法使用碱性磷酸酶作为报告酶，并将四唑鎓盐还原为 甲状腺作为检测/信号系统的一部分。

公开(公告)号：WO1996038584A1

公开(公告)日：1996-12-05

申请号：PCT/GB1996001296

申请日：1996-05-31

Applicant: CELSIS INTERNATIONAL PLC ,  GRANT, Peter, Leonard ,  FORSTER, Simon, James ,  THOMAS, Brian

Inventor： CELSIS INTERNATIONAL PLC

IPC: C12Q01/10

CPC classification number: C12Q1/045 ,  C12Q1/10 ,  C12Q2334/50 ,  C12Q2334/52

Abstract: In an assay for coliform or other microorganisms in a sample, which comprises contacting a mixture of the sample and a liquid growth medium with a material that undergoes a detectable change in the presence of the microorganisms to be assayed, the sample is contained in a well whose base is defined by a filter material, and the mixture is formed by placing the well on the growth medium. A preferred embodiment additionally comprises removing the sample and conducting a further assay adapted to distinguish between the microorganisms to be assayed and any other that may have given a false positive.

Abstract translation: 在样品中的大肠杆菌或其他微生物的测定中，其包括将样品和液体生长培养基的混合物与待测定的微生物存在下经历可检测变化的物质接触，将样品包含在孔中 其基底由过滤材料限定，并且通过将孔置于生长培养基上形成混合物。 优选的实施方案另外包括去除样品并进行适于区分要测定的微生物和可能具有假阳性的任何其它的另外的测定。

公开(公告)号：WO2008143722A3

公开(公告)日：2009-01-22

申请号：PCT/US2008001833

申请日：2008-02-12

Applicant: CHARM SCIENCES INC ,  SALTER ROBERT S ,  DURBIN GREGORY W

Inventor： SALTER ROBERT S ,  DURBIN GREGORY W

IPC: G01N33/569

CPC classification number: C12Q1/04 ,  C12Q2334/22 ,  C12Q2334/52

Abstract: Phage can be detected as rapid indicators of the hygienic quality of a sample. Both continuous flow methods and devices and single sample methods and devices, of various volumes, can be used. Test kits can be provided in easy-to-use formats. Certain phage, such as coliphage, are useful as indicators of fecal contamination.

Abstract translation: 可以将噬菌体作为样品卫生质量的快速指标。 可以使用不同体积的连续流动方法和装置以及单个样品方法和装置。 测试套件可以以易于使用的格式提供。 某些噬菌体，如大肠杆菌噬菌体，可用作粪便污染的指标。

公开(公告)号：WO00065033A1

公开(公告)日：2000-11-02

申请号：PCT/IL2000/000240

申请日：2000-04-24

IPC: G01N33/497 ,  C12N9/38 ,  C12N15/56 ,  C12Q1/34 ,  G01N33/50 ,  C12Q1/54 ,  G01N33/535

CPC classification number: C12Q1/34 ,  C12N9/2471 ,  C12Q2334/52 ,  C12Y302/01023

Abstract: The invention provides a method for the rapid assessment of the degree of halitosis comprising the steps of: a) obtaining a sample of fluid and/or tissue from the oral cavity of a subject, b) assessing the amount of beta -galactosidase present in said sample, c) determining the degree of halitosis in said subject, by comparing the result obtained in step b) with appropriate reference values.

Abstract translation: 本发明提供了一种用于快速评估口臭度的方法，包括以下步骤：a）从受试者的口腔获得流体和/或组织的样品，b）评估存在于所述的口腔中的β-半乳糖苷酶的量 样品，c）通过将步骤b）中获得的结果与适当的参考值进行比较来确定所述受试者的口臭程度。

公开(公告)号：WO1994017211A1

公开(公告)日：1994-08-04

申请号：PCT/US1994001224

申请日：1994-01-28

Applicant: WRIGHT, Dennis

IPC: C12Q01/68

CPC classification number: G01N33/535 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q2326/92 ,  C12Q2334/52 ,  G01N33/581 ,  G01N2333/924 ,  G01N2333/938 ,  Y02A50/57

Abstract: A non-radioactive method of detecting a ligand and antiligand complex labelled with alkaline phosphatase or a tracer having alkaline phosphatase conjugated thereto comprises reacting the complex with bromo-chloro-indolyl phosphate (BCIP), phenazine methosulfate (PMS) and dimethylthiazol diphenyl tetrazolium (MTT) and allowing the reaction to proceed to produce a colored formazan or a color change indicative of the presence of the labelled complex. A solution or composition of bromo-chloro-indolyl phosphate (BCIP), phenazine methosulfate (PMS) and dimethylthiazol diphenyl tetrazolium (MTT), as well as a test kit including the same, is also provided for carrying out the chromogenic method of detection.

Abstract translation: 检测用碱性磷酸酶标记的配体和反配体配合物的非放射性方法或与其结合的碱性磷酸酶的示踪剂包括使该配合物与溴 - 氯 - 吲哚基磷酸酯（BCIP），吩嗪甲硫酸盐（PMS）和二甲基噻唑二苯基四唑（MTT） ），并允许反应进行以产生着色甲an或指示存在标记复合物的颜色变化。 还提供溴 - 氯 - 吲哚磷酸盐（BCIP），吩嗪甲硫酸盐（PMS）和二甲基噻唑二苯基四唑（MTT）的溶液或组合物，以及包含该溶液或组合物的测试试剂盒，用于进行显色检测方法。

公开(公告)号：WO1992012259A1

公开(公告)日：1992-07-23

申请号：PCT/FR1991001075

申请日：1991-12-26

Applicant: BIO MERIEUX ,  MONGET, Daniel ,  VILLEVAL, François

Inventor： BIO MERIEUX

IPC: C12Q01/10

CPC classification number: C12Q1/10 ,  C12Q2334/52 ,  G01N2333/255 ,  Y02A50/59 ,  Y10S435/81 ,  Y10S435/975

Abstract: The invention relates to a method of bacteriological analysis for the selective detection of Salmonella and a medium for its implementation. The method is based on the specific capacity of Salmonella to ferment glucuronic acid or one of its salts, and not to produce beta -galactosidase. The medium comprises a nutrient which is metabolized by the bacteria, a chromogenic or fluorigenic compound capable of being hydrolyzed by the enzyme beta -galactosidase, glucuronic acid or one of its salts and a pH indicator.

公开(公告)号：WO1990012888A1

公开(公告)日：1990-11-01

申请号：PCT/US1990002346

申请日：1990-04-27

Applicant: BIOCONTROL SYSTEMS, INCORPORATED

Inventor： BIOCONTROL SYSTEMS, INCORPORATED ,  WARD, Robert, N., Jr. ,  DESROSIER, John, P. ,  MARSHALL, Elliott, D., III ,  FORD, Judith ,  MALLINAK, Nancy, J., S.

IPC: C12Q01/04

CPC classification number: C12Q1/04 ,  C12Q1/34 ,  C12Q2334/52 ,  G01N2333/924 ,  Y10S435/805 ,  Y10S435/97

Abstract: An improved method for testing for the presence of a particular microorganism and/or group of microorganisms characterized by a particular enzyme is provided. The method uses a dye-forming and/or fluorogenic-generating substrate throughout a polymer matrix or on the surface of a solid support member that forms a colored precipitate or fluorescent by-product when cleaved by the enzyme. The precipitate is concentrated on the polymer matrix and/or solid support member to create a visible reaction product, whereas the fluorescent by-product will normally be dispersed into the surrounding medium to some extent. The amount of dye-forming substrate needed is independent of the sample size. The present invention further comprises an enzyme indicator device comprising a dye-forming and/or fluorogenic-generating substrate throughout a polymer system or on the surface of a solid support member.

Abstract translation: 提供了一种用于测试特定微生物和/或由特定酶特征的微生物组的存在的改进方法。 该方法在整个聚合物基质中或在固体支持体的表面上使用形成染料形成和/或产生荧光的底物，其在被酶切割时形成着色沉淀物或荧光副产物。 将沉淀物浓缩在聚合物基质和/或固体支持构件上以产生可见的反应产物，而荧光副产物通常将在一定程度上分散到周围的介质中。 所需的染料形成衬底的量与样品尺寸无关。 本发明还包括一种酶指示剂装置，其在整个聚合物体系中或固体支持体的表面上包含染料形成和/或产生荧光的底物。