公开(公告)号：WO2015042201A3

公开(公告)日：2015-06-04

申请号：PCT/US2014056175

申请日：2014-09-17

申请人： ZYMOCHEM INC ,  CHOKHAWALA HARSHAL

发明人： CHOKHAWALA HARSHAL

IPC分类号： C12P7/40

CPC分类号： C12N15/52 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0016 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1205 ,  C12N9/13 ,  C12N9/16 ,  C12N9/2402 ,  C12N9/88 ,  C12N9/93 ,  C12P7/04 ,  C12P7/18 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P7/6409 ,  C12P13/001 ,  C12P13/005 ,  C12P17/08 ,  C12P17/10 ,  C12Y101/01001 ,  C12Y101/01002 ,  C12Y101/01035 ,  C12Y101/01078 ,  C12Y101/01268 ,  C12Y101/01269 ,  C12Y102/01003 ,  C12Y102/01005 ,  C12Y102/01022 ,  C12Y102/01024 ,  C12Y102/01026 ,  C12Y102/01048 ,  C12Y102/01063 ,  C12Y102/07005 ,  C12Y102/99006 ,  C12Y103/01044 ,  C12Y103/01045 ,  C12Y103/01086 ,  C12Y104/01 ,  C12Y203/01001 ,  C12Y203/01032 ,  C12Y203/01035 ,  C12Y203/01057 ,  C12Y206/01008 ,  C12Y206/01009 ,  C12Y206/01036 ,  C12Y206/01043 ,  C12Y206/01048 ,  C12Y206/01076 ,  C12Y206/01082 ,  C12Y207/01031 ,  C12Y207/01165 ,  C12Y208/03 ,  C12Y301/03 ,  C12Y301/03002 ,  C12Y301/03008 ,  C12Y301/03019 ,  C12Y301/0302 ,  C12Y302/01 ,  C12Y401/01001 ,  C12Y401/02 ,  C12Y401/02014 ,  C12Y401/0202 ,  C12Y401/02021 ,  C12Y401/03016 ,  C12Y401/03017 ,  C12Y402/01002 ,  C12Y402/01003 ,  C12Y402/01028 ,  C12Y402/0103 ,  C12Y402/01079 ,  C12Y402/0112 ,  C12Y602/01 ,  Y02E50/343

摘要： Provided herein are methods, compositions, and non-naturally occurring microbial organism for preparing compounds such as1-butanol, butyric acid, succinic acid, 1,4-butanediol, 1-pentanol, pentanoic acid, glutaric acid, 1,5-pentanediol, 1-hexanol, hexanoic acid, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, ε-Caprolactone, 6-amino-hexanoic acid, ε-Caprolactam, hexamethylenediamine, linear fatty acids and linear fatty alcohols that are between 7-25 carbons long, linear alkanes and linear -alkenes that are between 6-24 carbons long, sebacic acid and dodecanedioic acid comprising: a) converting a CN aldehyde and pyruvate to a CN+3 -hydroxyketone intermediate through an aldol addition; and b) converting the CN+3 -hydroxyketone intermediate to the compounds through enzymatic steps, or a combination of enzymatic and chemical steps.

摘要翻译： 本文提供的方法，组合物和非天然存在的微生物用于制备化合物，例如1-丁醇，丁酸，琥珀酸，1,4-丁二醇，1-戊醇，戊酸，戊二酸，1,5-戊二醇， 1-己醇，己酸，己二酸，1,6-己二醇，6-羟基己酸，ε-己内酯，6-氨基己酸，ε-己内酰胺，六亚甲基二胺，线性脂肪酸和线性脂肪醇， 长度为6-24个碳原子的直链烷烃和直链烷烃，癸二酸和十二烷二酸包括：a）通过醛醇缩合将CN醛和丙酮酸盐转化成CN + 3-羟基酮中间体; 和b）通过酶促步骤或酶和化学步骤的组合将CN + 3-羟基酮中间体转化为化合物。

公开(公告)号：WO2017035141A2

公开(公告)日：2017-03-02

申请号：PCT/US2016/048197

申请日：2016-08-23

申请人： WHITE DOG LABS, INC.

发明人： TRACY, Bryan P. ,  EYAL, Aharon M. ,  JONES, Shawn William ,  WIEDEL, Carrissa A.

IPC分类号： C12P7/28 ,  C12P7/56

CPC分类号： C12P7/28 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1217 ,  C12P7/04 ,  C12Y101/01001 ,  C12Y102/0101 ,  C12Y102/01057 ,  C12Y102/07005 ,  C12Y203/01019 ,  C12Y207/02007

摘要： The present invention provides microorganisms capable of converting acetyl-coA into crotyl alcohol as well as fermentation methods for producing crotyl alcohol, either alone, or in combination with acetone and/or isopropanol. The microorganisms may be genetically engineered to express and/or disrupt one or more of the following enzymes: acetaldehyde dehydrogenase, alcohol dehydrogenase, bifunctional acetaldehyde/alcohol dehydrogenase, aldehyde oxidoreductase, phosphotransacetylase, acetate kinase, CoA-transferase A, CoA-transferase B, acetoacetate decarboxylase, secondary alcohol dehydrogenase, butyryl-CoA dehydrogenase (BCD), and/or trans-2-enoyl-CoA reductase (TER).

摘要翻译： 本发明提供能够将乙酰-CoA转化为巴豆醇的微生物以及单独或与丙酮和/或异丙醇组合产生巴豆醇的发酵方法。 可以对微生物进行基因工程改造以表达和/或破坏一种或多种以下酶：乙醛脱氢酶，醇脱氢酶，双功能乙醛/醇脱氢酶，醛氧化还原酶，磷酸转乙酰酶，乙酸激酶，辅酶A转移酶A，辅酶A转移酶B， 乙酰乙酸脱羧酶，仲醇脱氢酶，丁酰-CoA脱氢酶（BCD）和/或反式-2-烯酰-CoA还原酶（TER）。

公开(公告)号：WO2016164339A3

公开(公告)日：2016-12-22

申请号：PCT/US2016025991

申请日：2016-04-05

申请人： SYNATA BIO INC

发明人： REEVES ANDREW ,  TOBEY RICHARD ,  ENZIEN MIKE

IPC分类号： C12P7/08 ,  C12P7/06 ,  C12P7/16

CPC分类号： C12P7/16 ,  C12P7/065 ,  C12P7/08 ,  C12P39/00 ,  C12R1/145 ,  C12Y102/07005 ,  Y02E50/10 ,  Y02E50/17

摘要： The invention relates to the fields of bacterial metabolism and the utilization or consumption of short-chain carboxylic acids to reduced products. Specifically, it relates to syngas fermentations using monocultures of syngas-utilizing homoacetogenic bacteria for the production of alcohols using native alcohol dehydrogenase.

摘要翻译： 本发明涉及细菌代谢领域和短链羧酸对还原产物的利用或消耗。 具体地说，本发明涉及使用合成气合成气合并嗜酸性细菌用于使用天然醇脱氢酶生产醇的合成气发酵。

公开(公告)号：WO2017066498A1

公开(公告)日：2017-04-20

申请号：PCT/US2016/056926

申请日：2016-10-13

申请人： LANZATECH NEW ZEALAND LIMITED ,  KOEPKE, Michael ,  OVERGAARD JENSEN, Rasmus ,  BEHRENDORFF, James Bruce Yarnton Haycock ,  HILL, Ryan Edward

发明人： KOEPKE, Michael ,  OVERGAARD JENSEN, Rasmus ,  BEHRENDORFF, James Bruce Yarnton Haycock ,  HILL, Ryan Edward

IPC分类号： C12N15/74 ,  C12N9/10 ,  C12N9/12 ,  C12P5/02 ,  C12P7/04 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44

CPC分类号： C12P7/42 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1217 ,  C12N9/16 ,  C12P5/007 ,  C12P5/026 ,  C12P7/04 ,  C12P7/18 ,  C12P7/24 ,  C12P7/28 ,  C12P7/625 ,  C12Y101/01001 ,  C12Y101/01002 ,  C12Y102/07005 ,  C12Y203/01008 ,  C12Y203/01009 ,  C12Y203/01019 ,  C12Y207/02001 ,  C12Y207/02007 ,  C12Y301/0202

摘要： The invention relates to a genetically engineered bacterium comprising an energy-generating fermentation pathway and methods related thereto. In particular, the invention provides a bacterium comprising a phosphate butyryltransferase (Ptb) and a butyrate kinase (Buk) (Ptb-Buk) that act on non-native substrates to produce a wide variety of products and intermediates. In certain embodiments, the invention relates to the introduction of Ptb-Buk into a C1-fixing microoorgansim capable of producing products from a gaseous substrate.

摘要翻译： 本发明涉及包含能量产生发酵途径的基因工程细菌及其相关方法。 具体而言，本发明提供了包含作用于非天然底物以产生多种产物和中间体的磷酸丁酰转移酶（Ptb）和丁酸激酶（Buk）（Ptb-Buk）的细菌。 在某些实施方案中，本发明涉及将Ptb-Buk引入到能够从气态底物生产产物的C1固定微生物群中。

公开(公告)号：WO2016164339A2

公开(公告)日：2016-10-13

申请号：PCT/US2016/025991

申请日：2016-04-05

申请人： SYNATA BIO, INC.

发明人： REEVES, Andrew ,  TOBEY, Richard ,  ENZIEN, Mike

CPC分类号： C12P7/16 ,  C12P7/065 ,  C12P7/08 ,  C12P39/00 ,  C12R1/145 ,  C12Y102/07005 ,  Y02E50/10 ,  Y02E50/17

摘要： The invention relates to the fields of bacterial metabolism and the utilization or consumption of short-chain carboxylic acids to reduced products. Specifically, it relates to syngas fermentations using monocultures of syngas-utilizing homoacetogenic bacteria for the production of alcohols using native alcohol dehydrogenase.

摘要翻译： 本发明涉及细菌代谢和利用或消耗短链羧酸以还原产物的领域。 具体而言，它涉及合成气发酵，其使用利用天然醇脱氢酶生产醇的利用合成气的单一合成气利用同源乙醇生成细菌。

公开(公告)号：WO2015042201A2

公开(公告)日：2015-03-26

申请号：PCT/US2014/056175

申请日：2014-09-17

申请人： ZYMOCHEM INC. ,  CHOKHAWALA, Harshal

发明人： CHOKHAWALA, Harshal

IPC分类号： C12P13/04 ,  C12N1/21

CPC分类号： C12N15/52 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0016 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1205 ,  C12N9/13 ,  C12N9/16 ,  C12N9/2402 ,  C12N9/88 ,  C12N9/93 ,  C12P7/04 ,  C12P7/18 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P7/6409 ,  C12P13/001 ,  C12P13/005 ,  C12P17/08 ,  C12P17/10 ,  C12Y101/01001 ,  C12Y101/01002 ,  C12Y101/01035 ,  C12Y101/01078 ,  C12Y101/01268 ,  C12Y101/01269 ,  C12Y102/01003 ,  C12Y102/01005 ,  C12Y102/01022 ,  C12Y102/01024 ,  C12Y102/01026 ,  C12Y102/01048 ,  C12Y102/01063 ,  C12Y102/07005 ,  C12Y102/99006 ,  C12Y103/01044 ,  C12Y103/01045 ,  C12Y103/01086 ,  C12Y104/01 ,  C12Y203/01001 ,  C12Y203/01032 ,  C12Y203/01035 ,  C12Y203/01057 ,  C12Y206/01008 ,  C12Y206/01009 ,  C12Y206/01036 ,  C12Y206/01043 ,  C12Y206/01048 ,  C12Y206/01076 ,  C12Y206/01082 ,  C12Y207/01031 ,  C12Y207/01165 ,  C12Y208/03 ,  C12Y301/03 ,  C12Y301/03002 ,  C12Y301/03008 ,  C12Y301/03019 ,  C12Y301/0302 ,  C12Y302/01 ,  C12Y401/01001 ,  C12Y401/02 ,  C12Y401/02014 ,  C12Y401/0202 ,  C12Y401/02021 ,  C12Y401/03016 ,  C12Y401/03017 ,  C12Y402/01002 ,  C12Y402/01003 ,  C12Y402/01028 ,  C12Y402/0103 ,  C12Y402/01079 ,  C12Y402/0112 ,  C12Y602/01 ,  Y02E50/343

摘要： Provided herein are methods, compositions, and non-naturally occurring microbial organism for preparing compounds such as1-butanol, butyric acid, succinic acid, 1,4-butanediol, 1-pentanol, pentanoic acid, glutaric acid, 1,5-pentanediol, 1-hexanol, hexanoic acid, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, ε-Caprolactone, 6-amino-hexanoic acid, ε-Caprolactam, hexamethylenediamine, linear fatty acids and linear fatty alcohols that are between 7-25 carbons long, linear alkanes and linear -alkenes that are between 6-24 carbons long, sebacic acid and dodecanedioic acid comprising: a) converting a C N aldehyde and pyruvate to a C N+3 -hydroxyketone intermediate through an aldol addition; and b) converting the C N+3 -hydroxyketone intermediate to the compounds through enzymatic steps, or a combination of enzymatic and chemical steps.

摘要翻译： 本文提供的方法，组合物和非天然存在的微生物用于制备化合物，例如1-丁醇，丁酸，琥珀酸，1,4-丁二醇，1-戊醇，戊酸，戊二酸，1,5-戊二醇， 1-己醇，己酸，己二酸，1,6-己二醇，6-羟基己酸，ε-己内酯，6-氨基己酸，ε-己内酰胺，六亚甲基二胺，直链脂肪酸和直链脂肪醇， 长度在7-25个碳原子之间，直链烷烃和6-24个碳原子的直链烷烃，癸二酸和十二烷二酸包括：a）通过醛醇缩合将CN醛和丙酮酸酯转化为CN + 3-羟基酮中间体; 和b）通过酶促步骤或酶和化学步骤的组合将CN + 3-羟基酮中间体转化为化合物。

公开(公告)号：WO2018079705A1

公开(公告)日：2018-05-03

申请号：PCT/JP2017/038875

申请日：2017-10-27

申请人： AJINOMOTO CO., INC.

发明人： TOYAZAKI, Miku ,  FUKUI, Keita

IPC分类号： C12N9/04

CPC分类号： C12P7/24 ,  C12N9/0008 ,  C12N9/1205 ,  C12Y102/07005

摘要： A method is described for producing an objective substance, for example, an aldehyde such as vanillin. The objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an ability to produce the objective substance, wherein the microorganism has been modified to have a specific carboxylic acid reductase (CAR) gene, such as a Gordonia CAR gene, Novosphingobium CAR gene, or Coccomyxa CAR gene.

摘要翻译： 描述了一种生产目标物质的方法，例如醛如香草醛。 通过使用具有产生目标物质的能力的微生物从目标物质的碳源或前体产生目标物质，其中微生物已经被修饰以具有特定的羧酸还原酶（CAR）基因，例如 Gordonia CAR基因，Novosphingobium CAR基因或Coccomyxa CAR基因。

公开(公告)号：WO2017035141A3

公开(公告)日：2017-04-06

申请号：PCT/US2016048197

申请日：2016-08-23

申请人： WHITE DOG LABS INC

发明人： TRACY BRYAN P ,  EYAL AHARON M ,  JONES SHAWN WILLIAM ,  WIEDEL CARRISSA A

IPC分类号： C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N15/52

CPC分类号： C12P7/28 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1217 ,  C12P7/04 ,  C12Y101/01001 ,  C12Y102/0101 ,  C12Y102/01057 ,  C12Y102/07005 ,  C12Y203/01019 ,  C12Y207/02007

摘要： The present invention provides microorganisms capable of converting acetyl-coA into crotyl alcohol as well as fermentation methods for producing crotyl alcohol, either alone, or in combination with acetone and/or isopropanol. The microorganisms may be genetically engineered to express and/or disrupt one or more of the following enzymes: acetaldehyde dehydrogenase, alcohol dehydrogenase, bifunctional acetaldehyde/alcohol dehydrogenase, aldehyde oxidoreductase, phosphotransacetylase, acetate kinase, CoA-transferase A, CoA-transferase B, acetoacetate decarboxylase, secondary alcohol dehydrogenase, butyryl-CoA dehydrogenase (BCD), and/or trans-2-enoyl-CoA reductase (TER).

摘要翻译： 本发明提供了能够将乙酰辅酶A转化为巴豆醇的微生物以及单独或与丙酮和/或异丙醇组合生产巴布醇的发酵方法。 微生物可以被遗传工程化以表达和/或破坏一种或多种以下酶：乙醛脱氢酶，醇脱氢酶，双官能乙醛/醇脱氢酶，醛氧化还原酶，磷酸转乙酰酶，乙酸激酶，辅酶A转移酶A，辅酶A转移酶B， 乙酰乙酸脱羧酶，仲醇脱氢酶，丁酰辅酶A脱氢酶（BCD）和/或反式-2-烯酰辅酶A还原酶（TER）。

公开(公告)号：WO2014197746A1

公开(公告)日：2014-12-11

申请号：PCT/US2014/041188

申请日：2014-06-05

申请人： LANZATECH NEW ZEALAND LIMITED ,  KOPKE, Michael ,  MUELLER, Alexander Paul

发明人： KOPKE, Michael ,  MUELLER, Alexander Paul

IPC分类号： C12P7/04 ,  C12P7/06 ,  C12P7/16

CPC分类号： C12P7/06 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1007 ,  C12N15/52 ,  C12N15/74 ,  C12P7/065 ,  C12P7/18 ,  C12Y101/01001 ,  C12Y102/0101 ,  C12Y102/07001 ,  C12Y102/07005 ,  C12Y201/01196 ,  Y02E50/17

摘要： The invention provides methods of increasing the production of fermentation products by increasing flux through a fermentation pathway by optimising enzymatic reactions. In particular, the invention relates to identifying enzymes and/or co-factors involved in metabolic bottlenecks in fermentation pathways, and fermenting a CO-comprising substrate with a recombinant carboxydotrophic Clostridia microorganism adapted to exhibit increased activity of the one or more of said enzymes, or increased availability of the one or more of said co-factors, when compared to a parental microorganism.

摘要翻译： 本发明提供了通过优化酶反应来增加通过发酵途径的通量来增加发酵产物的生产的方法。 特别地，本发明涉及鉴定涉及发酵途径中的代谢瓶颈的酶和/或辅因子，并且用重组羧基营养梭菌微生物发酵含CO底物，其适于显示一种或多种所述酶的活性增加， 或增加一种或多种所述辅因子的可用性，当与亲本微生物相比时。

公开(公告)号：WO2014018902A2

公开(公告)日：2014-01-30

申请号：PCT/US2013052346

申请日：2013-07-26

申请人： JOULE UNLTD TECHNOLOGIES INC

发明人： SKRALY FRANK A

IPC分类号： C12P7/08 ,  C12N1/21

CPC分类号： C12N9/0006 ,  C12N9/0008 ,  C12N9/88 ,  C12P7/065 ,  C12P7/40 ,  C12P19/40 ,  C12Y101/01037 ,  C12Y101/0104 ,  C12Y101/01082 ,  C12Y102/04001 ,  C12Y102/07005 ,  C12Y401/01003 ,  C12Y401/01031 ,  Y02E50/17

摘要： The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into pyruvate or acetyl-CoA, and in particular the use of such organisms for the commercial production of molecules derived from these precursors, e.g., ethanol.

摘要翻译： 本公开鉴定用于修饰光自养生物作为宿主的方法和组合物，使得所述生物将二氧化碳和光有效地转化为丙酮酸或乙酰-CoA，并且特别是使用这些生物用于商业生产源自这些前体的分子， 如乙醇。