公开(公告)号：WO2017016902A1

公开(公告)日：2017-02-02

申请号：PCT/EP2016/066875

申请日：2016-07-15

Applicant: EVONIK DEGUSSA GMBH

Inventor： HAAS, Thomas ,  THIESSENHUSEN, Anja ,  PÖTTER, Markus ,  BÜLTER, Thomas

IPC: C12P7/40 ,  C12P7/42 ,  C12N15/52 ,  C12N9/10 ,  C12N9/04 ,  C12N9/88

CPC classification number: C12P7/42 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/13 ,  C12N9/88 ,  C12N15/52 ,  C12P7/40 ,  C12Y101/01001 ,  C12Y203/01009 ,  C12Y208/03008 ,  C12Y401/01004

Abstract: There is provided amicrobial cell which is capable of producing acetoacetate, 3-hydroxybutyrate and/or 3-hydroxybutyrate variants, wherein the cell is genetically modified to comprise an increased expression relative to its wild type cell of: - an enzyme E 1 capable of catalysing the conversion of acetyl-CoA to acetoacetyl-CoA; - an enzyme E 2 capable of catalysing the conversion of acetoacetyl-CoA to acetoacetate; and -an enzyme E 3 capable of catalysing the conversion of acetoacetate to 3-hydroxybutyrate and/or variants thereof.

Abstract translation: 提供了能够产生乙酰乙酸酯，3-羟基丁酸酯和/或3-羟基丁酸酯变体的细菌细胞，其中细胞被遗传修饰以包含相对于其野生型细胞增加的表达： - 能够催化 乙酰辅酶A转化为乙酰乙酰辅酶A; - 能够催化乙酰乙酰辅酶A转化为乙酰乙酸的酶E2; 和能够催化乙酰乙酸酯转化为3-羟基丁酸酯和/或其变体的酶E3。

公开(公告)号：WO2016137976A1

公开(公告)日：2016-09-01

申请号：PCT/US2016/019102

申请日：2016-02-23

Applicant: BIOCHEMINSIGHTS, INC.

Inventor： DODDS, David R. ,  ARMIGER, William B. ,  KOFFAS, Mattheos

IPC: C12P1/00 ,  C12P7/16

CPC classification number: C12P7/16 ,  C12M45/07 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12Y101/01001 ,  C12Y101/01027 ,  C12Y101/01157 ,  C12Y102/0101 ,  C12Y102/01046 ,  C12Y102/01057 ,  C12Y102/04001 ,  C12Y103/01006 ,  C12Y103/01038 ,  C12Y203/01008 ,  C12Y203/01009 ,  C12Y203/01054 ,  C12Y401/02043 ,  C12Y503/01027 ,  C25B3/04 ,  C25B9/08 ,  C25B15/02 ,  C25B15/08 ,  Y02E50/10

Abstract: A combination of an electrochemical device for delivering reducing equivalents to a cell, and engineered metabolic pathways within the cell capable of utilizing the electrochemically provided reducing equivalents is disclosed. Such a combination allows the production of commodity chemicals by fermentation to proceed with increased carbon efficiency.

Abstract translation: 公开了一种用于递送还原当量至细胞的电化学装置和能够利用电化学提供的还原当量的细胞内工程化代谢途径的组合。 这种组合允许通过发酵生产商品化学品以提高碳效率。

公开(公告)号：WO2014052054A1

公开(公告)日：2014-04-03

申请号：PCT/US2013/059857

申请日：2013-09-16

Applicant: ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIVERSITY

Inventor： LEYH, Thomas, S.

IPC: C12P5/02 ,  C12P5/00 ,  C12P7/04

CPC classification number: C12P9/00 ,  C12P5/007 ,  C12Y101/01088 ,  C12Y203/01009 ,  C12Y203/0301 ,  C12Y207/01036 ,  C12Y207/04002 ,  C12Y602/01001

Abstract: Provided are method of synthesizing (R)-5-diphosphomevalonate (DPM), isopentenyl 5-pyrophosphase (IPP), dimethylallyl 5 -pyrophosphate (DMAPP) and isoprenoid using a one-pot synthesis.

Abstract translation: 提供了使用一锅合成合成（R）-5-二磷酸戊糖醛酸（DPM），异戊烯基-5-焦磷酸（IPP），5-丙基二甲基烯丙基酯（DMAPP）和类异戊二烯的方法。

公开(公告)号：WO2013019932A1

公开(公告)日：2013-02-07

申请号：PCT/US2012/049290

申请日：2012-08-02

Applicant: TRUSTEES OF DARTMOUTH COLLEGE ,  CHANG, Ta-yuan ,  CHANG, Catherine, C.Y. ,  HUANG, Paul, Li-Hau

Inventor： CHANG, Ta-yuan ,  CHANG, Catherine, C.Y. ,  HUANG, Paul, Li-Hau

IPC: C12N15/113 ,  A61K31/7088

CPC classification number: A61K31/713 ,  A61K31/7105 ,  A61K45/06 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2330/51 ,  C12Y203/01009

Abstract: The present invention includes methods for increasing white blood cell counts through administration of inhibitors of acyl -coenzyme A : cholesterol acyltransferase 1 (ACAT1). ACAT inhibitors are used to treat symptoms of immunosuppression, either alone or in combination with other treatments.

Abstract translation: 本发明包括通过施用酰基辅酶A：胆固醇酰基转移酶1（ACAT1）的抑制剂来增加白细胞计数的方法。 ACAT抑制剂用于单独或与其他治疗组合治疗免疫抑制症状。

公开(公告)号：WO2009145945A9

公开(公告)日：2010-02-04

申请号：PCT/US2009035331

申请日：2009-02-26

Applicant: BATTELLE ENERGY ALLIANCE LLC

Inventor： THOMPSON VICKI S ,  APEL WILLIAM A ,  REED DAVID W ,  LEE BRADY D ,  THOMPSON DAVID N ,  ROBERTO FRANCISCO ,  LACEY JEFFREY A

IPC: C12N9/02 ,  C12N9/00 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90 ,  C12N15/00

CPC classification number: C12N9/88 ,  C12N9/00 ,  C12N9/1029 ,  C12N15/74 ,  C12N15/75 ,  C12Y203/01009 ,  C12Y402/01055

Abstract: Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for modulating or altering metabolism in a cell using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

Abstract translation: 提供了分离和/或纯化的多肽和编码来自酸热环酸杆菌的多肽的核酸序列。 还提供了使用分离和/或纯化的多肽和酸性脂多酸杆菌的核酸序列调节或改变细胞代谢的方法。

公开(公告)号：WO2004052311A3

公开(公告)日：2005-04-14

申请号：PCT/US0339978

申请日：2003-12-09

Applicant: ISIS PHARMACEUTICALS INC ,  DOBIE KENNETH W

Inventor： DOBIE KENNETH W

IPC: A61K38/00 ,  C12N15/113 ,  C07H21/04 ,  A01N43/04 ,  A61K31/70

CPC classification number: C12N15/1137 ,  A61K38/00 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/3341 ,  C12N2310/341 ,  C12N2310/346 ,  C12Y203/01009 ,  C12N2310/3525

Abstract: Compounds, compositions and methods are provided for modulating the expression of acetyl-CoA acetyltransferase 2. The compositions comprise oligonucleotides, targeted to nucleic acid encoding acetyl-CoA acetyltransferase 2. Methods of using these compounds for modulation of acetyl-CoA acetyltransferase 2 expression and for diagnosis and treatment of disease associated with expression of acetyl-CoA acetyltransferase 2 are provided.

Abstract translation: 提供化合物，组合物和方法用于调节乙酰辅酶A乙酰转移酶2的表达。该组合物包含靶向编码乙酰辅酶A乙酰转移酶的核酸的寡核苷酸2.使用这些化合物调节乙酰辅酶A乙酰转移酶2表达和 提供了与乙酰辅酶A乙酰转移酶2表达相关的疾病的诊断和治疗。

公开(公告)号：WO2004052311A2

公开(公告)日：2004-06-24

申请号：PCT/US2003/039978

申请日：2003-12-09

Applicant: ISIS PHARMACEUTICALS, INC. ,  DOBIE, Kenneth, W.

Inventor： DOBIE, Kenneth, W.

IPC: A61K

CPC classification number: C12N15/1137 ,  A61K38/00 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/3341 ,  C12N2310/341 ,  C12N2310/346 ,  C12Y203/01009 ,  C12N2310/3525

Abstract: Compounds, compositions and methods are provided for modulating the expression of acetyl-CoA acetyltransferase 2. The compositions comprise oligonucleotides, targeted to nucleic acid encoding acetyl-CoA acetyltransferase 2. Methods of using these compounds for modulation of acetyl-CoA acetyltransferase 2 expression and for diagnosis and treatment of disease associated with expression of acetyl-CoA acetyltransferase 2 are provided.

Abstract translation: 提供化合物，组合物和方法用于调节乙酰辅酶A乙酰转移酶2的表达。该组合物包含靶向编码乙酰辅酶A乙酰转移酶的核酸的寡核苷酸2.使用这些化合物调节乙酰辅酶A乙酰转移酶2表达和 提供了与乙酰辅酶A乙酰转移酶2表达相关的疾病的诊断和治疗。

公开(公告)号：WO2017068385A1

公开(公告)日：2017-04-27

申请号：PCT/IB2015/002119

申请日：2015-10-23

Applicant: METABOLIC EXPLORER

Inventor： BESTEL-CORRE, Gwenaëlle ,  DUMON-SEIGNOVERT, Laurence ,  SOUCAILLE, Philippe

IPC: C12N1/21 ,  C12N9/10 ,  C12N9/00 ,  C12P7/52

CPC classification number: C12N9/1029 ,  C12N9/13 ,  C12N9/93 ,  C12P7/065 ,  C12P7/16 ,  C12P7/18 ,  C12P7/42 ,  C12P7/46 ,  C12P13/08 ,  C12P13/12 ,  C12Y203/01009 ,  C12Y203/01016 ,  C12Y208/03005 ,  C12Y208/03008 ,  C12Y602/01

Abstract: The present invention relates to a genetically modified microorganism for the fermentative conversion of levulinic acid into propionyl-CoA and acetyl-CoA, and to a fermentation process for performing said conversion.

Abstract translation: 本发明涉及用于将乙酰丙酸发酵转化成丙酰-CoA和乙酰-CoA的遗传修饰的微生物，并涉及用于进行所述转化的发酵过程。

公开(公告)号：WO2016090622A1

公开(公告)日：2016-06-16

申请号：PCT/CN2014/093667

申请日：2014-12-12

Applicant: 华东理工大学

Inventor： 王风清 ,  魏东芝 ,  徐丽琴 ,  刘浩浩 ,  刘心斌 ,  陈能辉

IPC: C12N15/53 ,  C12N15/54 ,  C12N1/21 ,  C12N9/02 ,  C12N9/10 ,  C12P33/00 ,  C12P33/02 ,  C12R1/32

CPC classification number: C12P33/02 ,  C12N9/0006 ,  C12N9/10 ,  C12N9/1029 ,  C12P33/00 ,  C12R1/32 ,  C12Y101/01035 ,  C12Y203/01009

Abstract: 提供一种羟酰辅酶A脱氢酶基因和一种酰基辅酶A硫解酶基因，及其基因工程菌和应用。所述羟酰辅酶A脱氢酶基因编码如下蛋白质(ⅰ)或(ⅱ)：(ⅰ)具有SEQ ID NO:2所示的氨基酸序列；(ⅱ)在(ⅰ)限定的氨基酸序列中经过取代，缺失或者叠加一个或几个氨基酸衍生的蛋白质且与(ⅰ)的蛋白质具有相同的功能。构建缺失羟酰辅酶A脱氢酶基因或酰基辅酶A硫解酶基因的分枝杆菌基因工程菌，并将其用于制备1，4-BNA、4-BNA、9-OH-BNA等甾体化合物。

公开(公告)号：WO2013178699A1

公开(公告)日：2013-12-05

申请号：PCT/EP2013/061103

申请日：2013-05-29

Applicant: NOVOZYMES A/S

Inventor： BAK, Torsten ,  JOERGENSEN, Steen ,  KOBMANN, Brian ,  CHRISTENSEN, Bjarke

IPC: C12N9/00 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N1/21 ,  C12P7/04 ,  C12N9/04 ,  C12R1/225

CPC classification number: C12Y203/01009 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1217 ,  C12N9/13 ,  C12N9/88 ,  C12P7/04 ,  C12Y101/0108 ,  C12Y207/02001 ,  C12Y208/03005 ,  C12Y401/01004

Abstract: Provided herein are Lactobacillusmutants having a disruption to an endogenous gene encoding an acetate kinase, wherein the mutants produce increased amounts of recombinant isopropanol. Also described aremethods forproducing the mutants and methods of using the mutants to produce isopropanol.

Abstract translation: 本文提供具有破坏编码醋酸激酶的内源基因的乳杆菌属，其中突变体产生增加量的重组异丙醇。 还描述了用于产生突变体的方法和使用突变体产生异丙醇的方法。