DEMUCILAGED FLAX SPROUTS AND THEIR BY-PRODUCT AS WELL AS PRODUCTION AND APPLICATION THEREOF
    2.
    发明申请
    DEMUCILAGED FLAX SPROUTS AND THEIR BY-PRODUCT AS WELL AS PRODUCTION AND APPLICATION THEREOF 审中-公开
    其生产和应用的分解性FLAX SPROUTS及其副产品

    公开(公告)号:WO2007029045A1

    公开(公告)日:2007-03-15

    申请号:PCT/HU2005/000095

    申请日:2005-09-06

    Inventor: FÖGLEIN, Ferenc

    Abstract: The present invention relates to demucilaged flax sprouts derived from flaxseeds freed of their mucilage. The flax sprouts according to the invention are easily digestible and can be utilized in different fields, e.g. in food industry, therapy and husbandry. The invention also relates to the production process and applications of the demucilaged flax sprouts. The present invention further relates to a process for recovering mucilaginous substance generated as a by-product in the production process as well as to various applications thereof.

    Abstract translation: 本发明涉及从其粘液中除去的亚麻籽衍生的破碎的亚麻芽。 根据本发明的亚麻芽很容易消化,并且可以用于不同的领域,例如, 在食品工业,治疗和畜牧业。 本发明还涉及破碎的亚麻芽的生产方法和应用。 本发明还涉及一种回收在生产过程中作为副产物产生的粘液物质及其各种应用的方法。

    METHODS FOR PURIFICATION OF ARYLSULFATASE A
    4.
    发明申请
    METHODS FOR PURIFICATION OF ARYLSULFATASE A 审中-公开
    芳烃酶A的纯化方法

    公开(公告)号:WO2014110246A1

    公开(公告)日:2014-07-17

    申请号:PCT/US2014/010856

    申请日:2014-01-09

    Abstract: The present invention provides, among other things, improved methods for purifying arylsulfatase A (ASA) protein produced recombinantly for enzyme replacement therapy. The present invention is, in part, based on the surprising discovery that recombinant ASA protein can be purified from unprocessed biological materials, such as, ASA-containing cell culture medium, using a process involving as few as four chromatography columns and only one step of post-chromatographic ultrafiltration/diafiltration.

    Abstract translation: 本发明尤其提供了用于纯化用于酶替代疗法重组产生的芳基硫酸酯酶A(ASA)蛋白质的改进方法。 本发明部分地基于令人惊奇的发现,即重组ASA蛋白可以从未加工的生物材料例如含ASA的细胞培养基中纯化,使用涉及至少四个色谱柱的方法,并且只有一个步骤 色谱后超滤/渗滤。

    アリールスルファターゼの活性測定方法
    6.
    发明申请
    アリールスルファターゼの活性測定方法 审中-公开
    测定蛋白酶活性的方法

    公开(公告)号:WO2012124668A1

    公开(公告)日:2012-09-20

    申请号:PCT/JP2012/056340

    申请日:2012-03-13

    Abstract:  アリールスルファターゼを、その基質であって当該基質がアリールスルファターゼの作用を受けると蛍光団又は発色団を遊離するものとを、イオン強度の高い水系反応系中で反応させる工程を含む、水系でのアリールスルファターゼの活性測定方法を提供する。また、FCC IV法によるラクターゼ活性が4,000NLU/g以上であり、ラクターゼ活性を基準として、本発明の蛍光法による水系でのアリールスルファターゼの活性測定方法で測定したアリールスルファターゼ活性が0.1%以下であるラクターゼ製剤と、この製剤の製造方法と、この製剤を用いてなる乳製品を提供する。

    Abstract translation: 提供了一种用于测定水性体系中芳基硫酸酯酶活性的方法,其包括在具有高离子强度的水性反应体系中使芳基硫酸酯酶与其底物反应的步骤,所述底物在受到荧光团或发色团的作用时释放 芳基硫酸酯酶。 还提供:具有4,000 NLU / g以上的乳糖酶活性的乳糖酶制剂,所述乳糖酶活性通过FCC IV法测定,芳基硫酸酯酶活性不大于0.1%,所述芳基硫酸酯酶活性通过荧光法测定,根据 基于乳糖酶活性测定水性体系中芳基硫酸酯酶活性的本发明; 一种制备所述制剂的方法; 以及使用所述制剂的乳制品。

    INACTIVATION OF OFF-TASTE INDUCING ENZYMES
    9.
    发明申请
    INACTIVATION OF OFF-TASTE INDUCING ENZYMES 审中-公开
    离子诱导酶的灭活

    公开(公告)号:WO2011070119A1

    公开(公告)日:2011-06-16

    申请号:PCT/EP2010/069295

    申请日:2010-12-09

    Inventor: BILLARD, Lionel

    Abstract: The present inventions relates to a method for the production of gellan gum, under mixing conditions, the method comprising a) providing a fermentation broth or other liquid medium containing gellan gum, b) if necessary adjusting the temperature and the pH of the fermentation broth/liquid medium to allow or facilitate enzymatic treatment in step c, c) adding one or more enzymes capable of reducing or abolishing the enzymatic activity of S. elodea derived arylsulfatase and/or β-glucuronidase, said one or more enzymes being added in an amount sufficient to reduce or abolish the enzymatic activity of S. elodea derived arylsulfatase and/or β-glucuronidase in the broth/liquid medium, and/or treating the broth/liquid medium at a temperature between 90°C and 125°C for a period of time sufficient to reduce or abolish the enzymatic activity of S. elodea derived arylsulfatase and/or β-glucuronidase in the broth/liquid medium, and d) optionally recovering the gellan gum from the gellan gum containing broth/liquid medium.

    Abstract translation: 本发明涉及在混合条件下生产结冷胶的方法,该方法包括:a)提供含有结冷胶的发酵液或其它液体培养基,b)如果需要,调节发酵液/ 液体培养基以允许或促进在步骤c中的酶处理,c)加入一种或多种能够降低或消除欧洲葡萄球菌衍生的芳基硫酸酯酶和/或β-葡糖醛酸糖苷酶的酶活性的酶,所述一种或多种酶加入量 足以减少或消除肉汤/液体培养基中的β-葡聚糖衍生的芳基硫酸酯酶和/或β-葡糖醛酸糖苷酶的酶活性,和/或在90℃至125℃的温度下处理培养液/液体培养基一段时间 的时间足以减少或消除肉汤/液体培养基中的β-葡聚糖衍生的芳基硫酸酯酶和/或β-葡糖醛酸糖苷酶的酶活性,和d)任选地从吉兰糖胶C中回收结冷胶 保持肉汤/液体培养基。

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