公开(公告)号：WO2016085929A1

公开(公告)日：2016-06-02

申请号：PCT/US2015/062324

申请日：2015-11-24

Applicant: CODEXIS, INC. ,  THE COCA-COLA COMPANY

Inventor： VROOM, Jonathan ,  ALVIZO, Oscar ,  SONI, Pankaj ,  BROWN, Michael, D. ,  ENTWISTLE, David

IPC: A23F5/00

CPC classification number: C12P17/182 ,  C07D473/12 ,  C12N9/1007 ,  C12N9/78 ,  C12Y305/04003

Abstract: The present invention provides an enzymatic means for the biosynthetic production of caffeine. The present invention provides biosynthetic methods for production of caffeine comprising: providing guanine, a guanine deaminase, at least one methyl transferase, and a methyl donor; contacting the guanine with the gtheuanine deaminase to produce xanthine; contacting the xanthine with the methyl transferase and a methyl donor, under conditions wherein the xanthine is methylated, to produce a monomethylxanthine; contacting the monomethylxanthine with the methyl transferase and a methyl donor, under conditions wherein the monomethylxanthine is methylated, to produce a dimethylxanthine; and contacting the dimethylxanthine with the methyl transferase and a methyl donor, under conditions wherein the dimethylxanthine is methylated, to produce caffeine (i.e., 1,3,7- trimethylxanthine).

Abstract translation: 本发明提供了用于生物合成咖啡因的酶法。 本发明提供生产咖啡因的生物合成方法，其包括：提供鸟嘌呤，鸟嘌呤脱氨酶，至少一种甲基转移酶和甲基供体; 使鸟嘌呤与肉毒梭菌脱氨酶接触以产生黄嘌呤; 在黄嘌呤被甲基化的条件下，将黄嘌呤与甲基转移酶和甲基供体接触以产生单甲基黄嘌呤; 在单甲基黄嘌呤被甲基化的条件下，将甲基黄嘌呤与甲基转移酶和甲基供体接触以产生二甲基黄嘌呤; 并在二甲基黄嘌呤被甲基化的条件下使二甲基黄嘌呤与甲基转移酶和甲基供体接触，生成咖啡因（即1,3,7-三甲基黄嘌呤）。

公开(公告)号：WO2015157431A2

公开(公告)日：2015-10-15

申请号：PCT/US2015/024943

申请日：2015-04-08

Applicant: NOVOGY, INC.

Inventor： GREENHAGEN, Emily H. ,  HAMILTON, Maureen ,  LaTOUF, William G. ,  CONSIGLIO, Andrew L. ,  MacEWEN, Kyle M. ,  SHAW, Arthur J., IV

IPC: C12P7/64 ,  C12N1/21

CPC classification number: C12P13/001 ,  C12N9/78 ,  C12N9/80 ,  C12N9/86 ,  C12N15/52 ,  C12N15/70 ,  C12N15/81 ,  C12P7/6463 ,  C12Y305/01054 ,  C12Y305/01084 ,  C12Y305/02015 ,  C12Y305/04 ,  C12Y305/04003 ,  C12Y305/99004

Abstract: Disclosed are transformed cells and related nucleotide and protein sequences, and fermentation compositions and methods, all of which are related to providing selective advantage in fermentation. For example, a selective advantage results from transformation of a cell with a nucleic acid that allows a transformed cell to metabolize one or more nitrogen-, phosphorous-, and/or sulfur-containing compounds that a native cell of the same species as the transformed cell cannot metabolize, and from fermentation of the transformed cell using one or more feedstocks, such as fractioned grain, which are depleted in or free of conventional nitrogen-, phosphorous-, and/or sulfur-containing compounds that a native cell of the same species as the transformed cell can metabolize. Also disclosed are methods for improved oxygen transfer in an aerobic or microaerobic fermentation.

Abstract translation: 公开了转化细胞和相关的核苷酸和蛋白质序列以及发酵组合物和方法，所有这些都与提供发酵选择性优势有关。 例如，选择性优势是由具有允许转化细胞代谢一种或多种含氮，磷和/或含硫化合物的核酸转化细胞而产生的，所述化合物是与转化的相同种类的天然细胞 细胞不能代谢，并且使用一种或多种原料（例如分馏的谷物）从转化细胞的发酵中分离出来，这些原料贫化或不含常规的含氮，磷和/或含硫化合物，这些化合物是相同的天然细胞 物种作为转化细胞可以代谢。 还公开了在需氧或微氧发酵中改善氧转移的方法。

公开(公告)号：WO2015075048A1

公开(公告)日：2015-05-28

申请号：PCT/EP2014/074967

申请日：2014-11-19

Applicant: DSM IP ASSETS B.V.

Inventor： RAEMAKERS-FRANKEN, Petronella Catharina ,  KIERKELS, Renier Henricus Maria

IPC: C12P17/12 ,  C12N9/78 ,  C07D251/46 ,  C07D251/52

CPC classification number: C12P17/12 ,  C12Y305/04003 ,  C12Y305/99003 ,  C12Y308/01008

Abstract: The present invention relates to a new process for the preparation of ammeline and/or ammelide from melamine by a solid-to-solid reaction in an aqueous reaction mixture comprising a biocatalyst, wherein the biocatalyst comprises at least one enzyme belonging to the amidohydrolase superfamily and having aminohydrolase activity towards 1,3,5-triazine compounds. The invention further relates a product obtainable by the process according to the invention, wherein the product comprises ammeline and/or ammelide.

Abstract translation: 本发明涉及一种通过在包含生物催化剂的水性反应混合物中通过固体 - 固体反应由三聚氰胺制备氨米林和/或丙烯酸酯的新方法，其中生物催化剂包含至少一种属于酰胺水解酶超家族的酶， 对1,3,5-三嗪化合物具有氨基水解酶活性。 本发明进一步涉及可通过本发明方法获得的产品，其中该产品包括氨米林和/或氨基甲酸酯。

公开(公告)号：WO2015157431A3

公开(公告)日：2015-12-03

申请号：PCT/US2015024943

申请日：2015-04-08

Applicant: NOVOGY INC

Inventor： GREENHAGEN EMILY H ,  HAMILTON MAUREEN ,  LATOUF WILLIAM G ,  CONSIGLIO ANDREW L ,  MACEWEN KYLE M ,  SHAW ARTHUR J IV

IPC: C12N15/00 ,  C12N1/00

CPC classification number: C12P13/001 ,  C12N9/78 ,  C12N9/80 ,  C12N9/86 ,  C12N15/52 ,  C12N15/70 ,  C12N15/81 ,  C12P7/6463 ,  C12Y305/01054 ,  C12Y305/01084 ,  C12Y305/02015 ,  C12Y305/04 ,  C12Y305/04003 ,  C12Y305/99004

Abstract: Disclosed are transformed cells and related nucleotide and protein sequences, and fermentation compositions and methods, all of which are related to providing selective advantage in fermentation. For example, a selective advantage results from transformation of a cell with a nucleic acid that allows a transformed cell to metabolize one or more nitrogen-, phosphorous-, and/or sulfur-containing compounds that a native cell of the same species as the transformed cell cannot metabolize, and from fermentation of the transformed cell using one or more feedstocks, such as fractioned grain, which are depleted in or free of conventional nitrogen-, phosphorous-, and/or sulfur-containing compounds that a native cell of the same species as the transformed cell can metabolize. Also disclosed are methods for improved oxygen transfer in an aerobic or microaerobic fermentation.

Abstract translation: 公开了转化细胞和相关的核苷酸和蛋白质序列，以及发酵组合物和方法，所有这些都涉及在发酵中提供选择性优势。 例如，选择性优势是由核酸转化细胞产生的，该核酸允许转化的细胞代谢一种或多种与转化物相同物种的天然细胞的含氮，含磷和/或含硫化合物 细胞不能代谢，并且使用一种或多种原料例如分馏的谷物发酵转化细胞，所述原料贫化或不含常规的含氮，含磷和/或含硫化合物，其天然细胞 作为转化细胞的物种可以代谢。 还公开了在好氧或微好氧发酵中改进氧气转移的方法。