公开(公告)号：WO2018013807A1

公开(公告)日：2018-01-18

申请号：PCT/US2017/041926

申请日：2017-07-13

Applicant: PALIN, William, J. ,  PALIN, Karen, A.

Inventor： PALIN, William, J. ,  PALIN, Karen, A.

IPC: G01N1/40

CPC classification number: G01N33/72 ,  G01N1/40 ,  G01N1/405 ,  G01N2001/4038 ,  G01N2333/445 ,  G01N2800/26

Abstract: An apparatus configured to enable the concentration of paramagnetic and/or superparamagnetic materials in a fluid for the purpose of examining such materials. The apparatus includes a magnet container arranged to be located proximate to a material container containing the materials to be examined. The magnet container includes therein a fluid and one or more magnets. The magnet container further includes means for controlling the movement of the one or more magnets in the fluid to allow the attraction of the paramagnetic and/or superparamagnetic materials in the material container to the one or more magnets as they travel in the magnet container so as to concentrate those materials. Several options for controlling the movement of the one or more magnets are described, including fluid viscosity selection, magnet shape and size selection, the use of an inclined pathway and the use of an inclined plane.

Abstract translation: 被配置为使流体中顺磁和/或超顺磁材料的浓度能够用于检查这种材料的装置。 该设备包括磁体容器，该磁体容器布置成靠近包含待检查材料的材料容器定位。 磁体容器在其中包括流体和一个或多个磁体。 磁体容器还包括用于控制流体中的一个或多个磁体的运动的装置，以允许材料容器中的顺磁和/或超顺磁材料在磁体容器中行进时吸引到一个或多个磁体，以便 集中这些材料。 描述了用于控制一个或多个磁体的移动的若干选项，包括流体粘度选择，磁体形状和尺寸选择，使用倾斜路径和使用倾斜平面。

公开(公告)号：WO2017193126A1

公开(公告)日：2017-11-09

申请号：PCT/US2017/031557

申请日：2017-05-08

Applicant: THE GENERAL HOSPITAL CORPORATION

Inventor： IRIMIA, Daniel ,  ELLETT, Felix ,  JORGENSEN, Julianne

IPC: B01L3/00 ,  G01N33/48 ,  G01N33/49

CPC classification number: B01L3/502753 ,  B01L2200/0694 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/086 ,  G01N15/0205 ,  G01N15/1031 ,  G01N15/1056 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/1484 ,  G01N33/487 ,  G01N33/5091 ,  G01N33/54366 ,  G01N2015/1006 ,  G01N2015/1075 ,  G01N2015/1488 ,  G01N2015/1493 ,  G01N2800/26

Abstract: The disclosure relates to microfluidic devices and methods of use thereof for monitoring the directionality, velocity, and migration persistence of neutrophils or other cells in the absence of chemical gradients for the purposes of detecting and quantifying abnormal neutrophil motility phenotypes, using low sample volumes and with minimal activation of the neutrophils. The devices and methods can be used to diagnose sepsis in subjects suspected of having sepsis or at risk of developing sepsis. The devices and methods can also be used to monitor the responses of patients to sepsis therapies.

Abstract translation: 本公开涉及微流体装置及其使用方法，用于在缺乏化学梯度的情况下监测嗜中性粒细胞或其他细胞的方向性，速度和迁移持久性，用于检测和量化异常嗜中性粒细胞运动性 表型，使用低样本量和嗜中性粒细胞的最小活化。 这些装置和方法可用于诊断怀疑患有脓毒症或处于发展败血症风险的受试者的脓毒症。 该设备和方法也可用于监测患者对败血症治疗的反应。

公开(公告)号：WO2017156436A1

公开(公告)日：2017-09-14

申请号：PCT/US2017/021873

申请日：2017-03-10

Applicant: S&R PHARMACEUTICALS, LLC

Inventor： HSU, Tsungda

IPC: G01N33/558 ,  C12Q1/68 ,  G01N21/00 ,  G01N33/483 ,  G01N33/569

CPC classification number: G01N33/5695 ,  C12Q1/689 ,  G01N33/558 ,  G01N2469/10 ,  G01N2800/26

Abstract: Provided herein is an assay for detecting a rough-type mycobacterium and a smooth-type mycobacterium in a sample, wherein the mycobacterium is Mycobacterium tuberculosis or Mycobacterium bovis . The assay comprises a first molecule that selectively binds to the rough- type mycobacterium or binds to a molecule preferentially secreted by the rough-type mycobacterium and a second molecule that selectively binds to the smooth-type mycobacterium or binds to a molecule preferentially secreted by the smooth-type mycobacterium. The first molecule and the second molecule are independently detectable. Also provided herein are corresponding methods for detecting a rough-type mycobacterium and a smooth-type mycobacterium in a sample and assays and methods for detecting tuberculosis infection in a sample.

Abstract translation: 本文提供了用于检测样品中的粗糙型分枝杆菌和平滑型分枝杆菌的测定法，其中所述分枝杆菌是结核分枝杆菌或牛分枝杆菌（M. Mycobacterium bovis） / i>的。 该测定包括选择性结合至粗糙型分枝杆菌或与粗糙型分枝杆菌优先分泌的分子结合的第一分子和选择性结合至平滑型分枝杆菌或结合至由所述粗糙型分枝杆菌优先分泌的分子的第二分子 平滑型分枝杆菌。 第一分子和第二分子是可独立检测的。 本文还提供了用于检测样品中的粗糙型分枝杆菌和平滑型分枝杆菌以及用于检测样品中的结核感染的分析方法和方法的相应方法。

公开(公告)号：WO2017151794A1

公开(公告)日：2017-09-08

申请号：PCT/US2017/020240

申请日：2017-03-01

Applicant: ROWAN UNIVERSITY

Inventor： SHAHI, Alisina ,  PARVIZI, Javad

IPC: C12Q1/68 ,  G01N33/53 ,  G01N33/68

CPC classification number: C12Q1/68 ,  G01N33/6893 ,  G01N2800/26 ,  G01N2800/52

Abstract: This invention relates to the detection and quantification of D-dimer levels in a sample derived from a patient having or suspected of having periprosthetic joint infection (PJI) and subsequent diagnosis of PJI.

Abstract translation: 本发明涉及来源于患有或怀疑患有假体周围关节感染（PJI）并随后诊断PJI的患者的样品中D-二聚体水平的检测和定量。

公开(公告)号：WO2017103001A3

公开(公告)日：2017-08-10

申请号：PCT/EP2016081283

申请日：2016-12-15

Applicant: INSERM (INSTITUT NAT DE LA SANTÉ ET DE LA RECH MÉDICALE) ,  ASSIST PUBLIQUE-HÔPITAUX DE PARIS (APHP) ,  UNIVERSITÉ PARIS-EST CRÉTEIL VAL DE MARNE

Inventor： HUE SOPHIE ,  LEVY YVES

IPC: G01N33/68

CPC classification number: G01N33/6893 ,  G01N2333/4737 ,  G01N2333/5412 ,  G01N2333/70596 ,  G01N2800/24 ,  G01N2800/26 ,  G01N2800/50 ,  G01N2800/52

Abstract: The invention provides diagnostic markers of immunosenescence and methods of identifying individuals with impaired immune function based on the expression level of a combination of such markers in a biological sample obtained from said individual. Such combination of markers is useful for determining the susceptibility to nosocomial infections of an individual. Such combination of markers is also useful for predicting whether an individual will respond to active vaccination and become protected against recurring diseases.

Abstract translation: 本发明提供了免疫衰老的诊断标志物和基于从所述个体获得的生物样品中此类标志物组合的表达水平鉴定免疫功能受损个体的方法。 标记物的这种组合对于确定个体的医院感染的易感性是有用的。 标记物的这种组合也可用于预测个体是否会对主动疫苗接种作出反应，并预防其对复发性疾病的保护。

公开(公告)号：WO2017106918A1

公开(公告)日：2017-06-29

申请号：PCT/AU2016/051269

申请日：2016-12-22

Applicant: IMMUNEXPRESS PTY LTD

Inventor： BRANDON, Richard Bruce ,  FOX, Brian Andrew ,  MCHUGH, Leo Charles ,  SAMPSON, Dayle Lorand

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q2600/158 ,  G01N33/6893 ,  G01N2800/26

Abstract: Disclosed are methods, apparatus, kits and compositions for determining the absence of a systemic bacterial infection (sepsis) in patients, particularly ones presenting to hospital emergency departments (ED) as outpatients, by measurement of the host immune response using peripheral blood. The are methods, apparatus, kits and compositions can be used in mammals for diagnosing, making treatment decisions, determining the next procedure or diagnostic test, or management of patients suspected of having an infection, including those presenting with fever or other signs of systemic inflammation. More particularly, peripheral blood RNA and protein biomarkers are disclosed that are useful for distinguishing between the host immune response to bacteria compared to the host immune response to other causes of systemic inflammation including trauma, burns, autoimmune disease, asthma, anaphylaxis, arthritis, obesity and viral infections. As such, the biomarkers are useful for distinguishing bacterial-associated systemic inflammatory response syndrome from non-bacterial systemic inflammation to provide clinicians with strong negative predictive value (>95%) so that sepsis can be excluded as a diagnosis in patients presenting to ED with clinical signs of systemic inflammation.

Abstract translation: 公开了用于确定患者中不存在全身细菌感染（败血症）的方法，设备，试剂盒和组合物，所述患者特别是作为门诊患者提供给医院急诊科（ED）的患者，通过测量宿主 使用外周血的免疫应答。 这些方法，装置，试剂盒和组合物可用于哺乳动物中，用于诊断，作出治疗决定，确定下一步骤或诊断测试，或疑似患有感染的患者的处理，包括出现发热或其他全身炎症迹象的患者 。 更具体地，公开了外周血RNA和蛋白质生物标志物，其可用于区分与宿主对全身性炎症的其他原因（包括创伤，烧伤，自身免疫性疾病，哮喘，过敏性反应，关节炎，肥胖症）的免疫反应相比对宿主的免疫反应 和病毒感染。 因此，生物标记可用于将细菌相关的全身炎症反应综合征与非细菌性全身性炎症区分开来，从而为临床医生提供强烈的阴性预测值（> 95％），从而可以排除脓毒症作为ED患者中的诊断 伴有全身炎症的临床体征。

公开(公告)号：WO2017099445A1

公开(公告)日：2017-06-15

申请号：PCT/KR2016/014215

申请日：2016-12-06

Applicant: 대한민국(관리부서:국립수산과학원)

Inventor： 김명석 ,  정승희 ,  최혜승 ,  한현자 ,  도정완 ,  김진도

IPC: C12Q1/68

CPC classification number: C12Q1/04 ,  C12Q1/6809 ,  C12Q1/6888 ,  C12Q1/689 ,  C12Q2525/107 ,  C12Q2527/107 ,  G01N33/56911 ,  G01N2800/26

Abstract: 본 발명은 어류 에드와드 감염증과 연쇄구균 감염증 원인세균의 판별 및 검출용 유전자 마커, 및 이를 이용한 원인세균의 판별 및 검출 방법에 관한 것으로, 더욱 자세하게는 어류 에드와드 감염증과 연쇄구균 감염증 원인세균인 에드와드시엘라 타르다(Edwardsiella tarda), 연쇄구균인 스트렙토코카스 이니애( Streptococcus iniae ), 스트렙토코카스 파라우베리스( Streptococcus parauberis ) 및 락토코카스 가비에( Lactococcus garvieae )의 16S rDNA 유전자를 코딩하는 DNA 염기서열 중 세균 특이적 단일염기다형성(Single nucleotide polymorphism, SNP)을 포함하는 유전자 마커를 선정하여 증폭시킨 다음, 증폭산물을 특이적으로 인식하는 펩티드핵산(Peptide Nucleic Acid, PNA)을 혼성화시키고, 혼성화된 산물의 온도 조절을 통해 온도별 융해곡선을 얻고, 상기 얻은 융해곡선의 분석을 통해 융해온도로부터 세균 종을 판별하거나 어류의 상기 세균 종의 감염 여부를 검출하는 방법에 관한 것이다. 본 발명은 어류 질병 원인세균인 에드와드시엘라 타르다, 스트렙토코카스 이니애, 스트렙토코카스 파라우베리스 및 락토코카스 가비에의 판별 및/또는 검출용 유전자 마커를 선정하고, 상기 유전자 마커에 특이적인 펩티드핵산 및 프라이머 쌍을 이용하여 세균 종마다 서로 다른 형광의 증폭 및 융해곡선을 나타내게 함으로써 어류 질병 원인세균을 간단ㆍ신속ㆍ정확하게 판별하고, 어류의 상기 세균의 감염 여부를 검출할 수 있는 효과가 있다.

Abstract translation:

本发明是鱼埃德病房感染和链球菌感染引起的细菌的确定和检测用于遗传标记，并涉及确定和使用相同的，更具体地鱼导致细菌的检测方法，爱德病房感染 和链球菌感染引起的细菌，ED和吃艾拉焦油（爱德华氏菌），链霉菌属的上午链球菌kokaseu柯（海豚链球菌的），链霉素kokaseu对宇部更小（链球菌parauberis ）和乳杆菌kokaseu垃圾（格氏乳球菌的）编码敌人多态性的16S rDNA基因（遗传标志物，包括单核苷酸多态性，SNP的DNA序列的特定细菌）在 然后将扩增杂交肽核酸（肽核酸，PNA），其识别出的扩增产物以特定和，以获得与杂交产物的温度控制在特定的温度熔融曲线，将得到的融合的歌曲 确定从熔融温度的细菌物种通过线分析，或用于检测感染鱼的细菌物种的方法。 具体在鱼病致病菌的肽本发明中，爱德和一定艾拉焦油，链霉菌属kokaseu上午悬崖，链霉菌属kokaseu对宇部以下，乳杆菌确定和/或检测和选择的基因标记物的，该kokaseu加维的基因标记 有，使用核酸和引物对细菌物种用于通过呈现不同荧光的扩增曲线和熔融曲线简化鱼病致病菌和快速和准确地确定每个彼此，并且能够检测感染在鱼的细菌的效果。