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    METHOD FOR PROFILING PROTEIN METHYLATION
    3.
    发明申请
    METHOD FOR PROFILING PROTEIN METHYLATION 审中-公开
    配方蛋白甲基化方法

    公开(公告)号：WO2016205943A1

    公开(公告)日：2016-12-29

    申请号：PCT/CA2016/050736

    申请日：2016-06-23

    Applicant: UNIVERSITY OF OTTAWA

    Inventor： FIGEYS, Daniel ,  NING, Zhibin ,  MIERZWA, Anna Sylvia

    IPC: G01N33/483 ,  C12Q1/37 ,  C40B20/08 ,  G01N30/96

    CPC classification number: G01N33/6812 ,  C12N9/1007 ,  C40B20/08 ,  G01N30/14 ,  G01N30/96 ,  G01N2030/8831 ,  G01N2440/12

    Abstract: There is provided a method for identifying protein methylation on arginine and lysine residues. The method comprises obtaining a set of peptides; blocking un- methylated arginine and lysine residues and the free N-terminal amine of peptides in the set of peptides, so that un-methylated peptides are neutralized and only methylated peptides are positively charged at neutral or basic pH; isolating the methylated peptides based on charge; and performing mass spectrometry (MS) analysis on the isolated methylated peptides to detect methylated lysine and arginine residues. Methods provided herein can be used for large scale, high throughput profiling of protein methylation in a cell or tissue.

    Abstract translation: 提供了一种在精氨酸和赖氨酸残基上鉴定蛋白质甲基化的方法。 该方法包括获得一组肽; 阻断未甲基化的精氨酸和赖氨酸残基以及所述肽组中肽的游离N-末端胺，使得未甲基化的肽被中和，并且只有甲基化肽在中性或碱性pH下带正电荷; 基于电荷分离甲基化肽; 并对分离的甲基化肽进行质谱（MS）分析，以检测甲基化赖氨酸和精氨酸残基。 本文提供的方法可用于细胞或组织中蛋白质甲基化的大规模，高通量分析。

    METHOD AND APPARATUS FOR PRODUCING SEQUENCE VERIFIED DNA
    4.
    发明申请
    METHOD AND APPARATUS FOR PRODUCING SEQUENCE VERIFIED DNA 审中-公开
    用于产生序列验证的DNA的方法和装置

    公开(公告)号：WO2014179735A1

    公开(公告)日：2014-11-06

    申请号：PCT/US2014/036645

    申请日：2014-05-02

    Applicant: CAMBRIAN GENOMICS, INC.

    Inventor： HEINZ, Austen ,  LEVSKAYA, Anselm ,  MULLIGAN, John

    IPC: C40B20/08 ,  C40B40/06 ,  C12M1/34 ,  C12Q1/68

    CPC classification number: C12N15/1065 ,  B01J2219/005 ,  B01J2219/00572 ,  B01J2219/00596 ,  C40B20/08 ,  C40B50/14

    Abstract: A method of retrieving a subset of polynuceotide molecules from a mixture of polynucleotide molecules includes receiving a mixture of nucleotide sequences comprising one or more polynucleotide molecules, synthesizing one or more identifier (ID) regions onto the one or more polynucleotide molecules. The method also includes generating a bead-bound library of one or more beads comprising subsets of identical polynucleotide molecules. Each bead is identified by the ID Sequence of the associated Polynucleotide Sequence. The method further includes sequencing the one or more ID regions of each bead to generate ID Sequence information for each bead, combining the Polynucleotide Sequence information, the one or more ID Sequences, and coordinates of each bead to identify the Polynucleotide Sequence on the bead, and retrieving the bead with its associated Polynucleotide Sequence from the flow cell based on the absolute coordinate position of the bead.

    Abstract translation: 从多核苷酸分子的混合物中检索多肽核苷酸分子子集的方法包括接收包含一个或多个多核苷酸分子的核苷酸序列的混合物，在一个或多个多核苷酸分子上合成一个或多个标识符（ID）区域。 该方法还包括产生包含相同多核苷酸分子亚组的一个或多个珠的珠结合文库。 每个珠粒由相关多核苷酸序列的ID序列鉴定。 该方法还包括对每个珠粒的一个或多个ID区域进行排序以产生每个珠粒的ID序列信息，组合多核苷酸序列信息，一个或多个ID序列和每个珠粒的坐标以鉴定珠粒上的多核苷酸序列， 并且基于珠的绝对坐标位置从流动池中取出具有其相关多核苷酸序列的珠粒。

    BIOMARKER DISCOVERY IN COMPLEX BIOLOGICAL FLUID USING BEAD OR PARTICLE BASED LIBRARIES AND DIAGNOSTIC KITS AND THERAPEUTICS
    6.
    发明申请
    BIOMARKER DISCOVERY IN COMPLEX BIOLOGICAL FLUID USING BEAD OR PARTICLE BASED LIBRARIES AND DIAGNOSTIC KITS AND THERAPEUTICS 审中-公开
    生物识别在复杂生物流体中的应用基于珠粒或基于颗粒的图书馆和诊断套件及治疗方法

    公开(公告)号：WO2012129423A2

    公开(公告)日：2012-09-27

    申请号：PCT/US2012/030161

    申请日：2012-03-22

    Applicant: OPKO PHARMACEUTICALS, LLC ,  MOOLA, Muralidhar, Reddy ,  SCHILKE, Jessica

    Inventor： MOOLA, Muralidhar, Reddy ,  SCHILKE, Jessica

    IPC: C40B20/08 ,  C40B40/10 ,  G01N33/543 ,  C40B30/04 ,  G01N33/574 ,  C07K7/06

    CPC classification number: G01N33/6845 ,  C07K1/047 ,  C07K7/06 ,  C40B40/04 ,  C40B40/10 ,  C40B60/12 ,  G01N33/566 ,  G01N33/57438 ,  G01N2800/104 ,  G01N2800/2821

    Abstract: The present invention is useful in screening for biomarkers associated with any other disease or condition. Such diseases and conditions range from the neurological diseases, autoimmune diseases and cancers identified above as well as any other disease or condition that has a biomarker such as an antibody or other characterizing protein or biomolecule associated with the disease or progression of the disease. The large ligand libraries of the invention can be used directly in biological fluid, under the appropriate experimental conditions and according to the processes recited herein, to screen for such markers and without the need to use fewer support members (e.g. about 100,000 or less) or without the need to transfer such peptoids or ligands to a microarray before screening the biological fluid. In addition, the ligand libraries may also be used to screen for cell based receptors that specifically relate to a particular cell surface marker.

    Abstract translation: 本发明可用于筛选与任何其它疾病或病症相关的生物标志物。 这些疾病和病症范围从神经系统疾病，自身免疫性疾病和上述癌症以及具有生物标志物如抗体或其它特征性蛋白质或与该疾病或疾病进展相关的生物分子的任何其它疾病或病症。 本发明的大配体文库可以在适当的实验条件下，并根据本文所述的方法直接用于生物流体，以筛选这些标记物，而不需要使用较少的支持成员（例如约100,000或更少）或 而不需要在筛选生物流体之前将这些类似物或配体转移到微阵列。 此外，配体文库也可用于筛选特异性涉及特定细胞表面标志物的基于细胞的受体。

    SAMPLE ANALYSIS BY MASS CYTOMETRY
    8.
    发明申请
    SAMPLE ANALYSIS BY MASS CYTOMETRY 审中-公开
    样品分析通过大量细胞计数

    公开(公告)号：WO2014063246A1

    公开(公告)日：2014-05-01

    申请号：PCT/CA2013/050797

    申请日：2013-10-22

    Applicant: FLUIDIGM CANADA INC.

    Inventor： TANNER, Scott

    IPC: G01N27/00 ,  C12M1/34 ,  G01N33/483 ,  G01N33/58 ,  C40B20/08 ,  C40B70/00 ,  H01J49/40

    CPC classification number: G01N27/626 ,  G01N1/04 ,  G01N2001/045 ,  H01J49/0031 ,  H01J49/0418

    Abstract: In a mass cytometer system, a tissue sample labeled with multiple metal tags is supported on an encoded substrate for distribution profile mapping by laser ablation. Groups of elemental ions from each plume generated by each laser pulse are detected by the mass cytometer and the data is mapped according to the encoded substrate. This configuration allows for the production of a 3-dimentional distribution profile of the multiple metal tags in the tissue sample.

    Abstract translation: 在质谱仪系统中，通过激光烧蚀在经编码的衬底上支持用多个金属标签标记的组织样品用于分布轮廓图。 通过质谱仪检测由每个激光脉冲产生的每个羽流的元素离子组，并且根据编码的底物映射数据。 这种构造允许生成组织样品中多个金属标签的3维分布轮廓。

    PEPTIDE LIBRARIES FOR SCREENING AND OTHER APPLICATIONS
    10.
    发明申请
    PEPTIDE LIBRARIES FOR SCREENING AND OTHER APPLICATIONS 审中-公开
    用于筛选和其他应用的肽图书馆

    公开(公告)号：WO2012026887A1

    公开(公告)日：2012-03-01

    申请号：PCT/SG2011/000298

    申请日：2011-08-29

    Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH ,  LEE, Su Seong ,  LIM, Jaehong ,  CHA, Junhoe ,  ANG, Yi Li ,  ZHENG, Yiran ,  TAN, Sylvia

    Inventor： LEE, Su Seong ,  LIM, Jaehong ,  CHA, Junhoe ,  ANG, Yi Li ,  ZHENG, Yiran ,  TAN, Sylvia

    IPC: C40B20/04 ,  C40B20/08 ,  C40B50/16 ,  C07K1/04 ,  C40B30/10 ,  C07K17/00 ,  C40B40/10

    CPC classification number: G01N33/6818 ,  C07K1/047 ,  C07K1/13 ,  C07K7/06 ,  C40B20/08 ,  C40B40/04 ,  C40B50/18 ,  C40B70/00

    Abstract: The present invention generally relates to various peptides and particles, for example, for use in screening of particle- or bead-based peptide libraries. In one aspect, the present invention is generally directed to articles including peptides attached to one or more particles, which may have structures such as (particle)-M-Q-Z n -X-J, (particle)-M-Q-Z n -X 1 -X 2 -X 3 -X 4 -X 5 -J, (particle)-M-R-Z n -X 1 -X 2 -X 3 -X 4 -X 5 -J, (particle)-M-R- - X 1 -X 2 -X 3 -X 4 -X 5 -Z n -Q-J, (particle)-M-X 1 -X 2 -X- 3 X 4 -X 5 -Z n -R-J, etc., where M is a methionine residue, M1 is a cleavable linker residue, Q is a group able to enhance intensity and/or sensitivity of mass spectrometry, X comprises one or more amino acid residues, n is a positive integer, Z is a covalent bond or a spacer, and J is an endgroup. In some embodiments, the spacer may comprise a structure such as:(I). Other aspects of the present invention generally relate to methods of using such articles, e.g., by exposing the article to a target molecule such as a protein, for example, for use in screening of particle-based peptide libraries. Still other aspects of the present invention generally relate to methods of making such articles, methods of promoting such articles, kits involving such articles, or the like.

    Abstract translation: 本发明通常涉及各种肽和颗粒，例如用于筛选基于颗粒或珠的肽文库。 一方面，本发明一般涉及包含连接到一个或多个颗粒上的肽的制品，其可以具有诸如（颗粒）-MQ-Zn-XJ，（颗粒）-MQ-Zn-X1-X2-X3 -X4-X5-J，（颗粒）-MR-Zn-X1-X2-X3-X4-X5-J，（颗粒）-MR-X1-X2-X3-X4-X5-Zn-QJ ）-M-X1-X2-X-3X4-X5-Zn-RJ等，其中M是甲硫氨酸残基，M1是可切割的接头残基，Q是能够增强质谱的强度和/或灵敏度的基团 X包含一个或多个氨基酸残基，n为正整数，Z为共价键或间隔基，J为端基。 在一些实施方案中，间隔物可以包含如下结构：（I）。 本发明的其它方面通常涉及使用这种制品的方法，例如通过将制品暴露于靶分子例如蛋白质，例如用于筛选基于颗粒的肽文库。 本发明的其它方面通常涉及制造这种制品的方法，促进这种制品的方法，涉及这种制品的试剂盒等。

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