- 专利标题: Massive parallel method for decoding DNA and RNA
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申请号: US15915983申请日: 2018-03-08
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公开(公告)号: US10669582B2公开(公告)日: 2020-06-02
- 发明人: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
- 申请人: The Trustees of Columbia University in the City of New York
- 申请人地址: US NY New York
- 专利权人: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
- 当前专利权人: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
- 当前专利权人地址: US NY New York
- 代理机构: Cooper & Dunham LLP
- 代理商 John P. White
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C07H19/14 ; C12Q1/6869 ; C07H21/00 ; C12Q1/686 ; C12Q1/6874 ; C12Q1/6872 ; C07H19/10 ; C12Q1/6876 ; C40B40/00
摘要:
This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
公开/授权文献
- US20180201642A1 MASSIVE PARALLEL METHOD FOR DECODING DNA AND RNA 公开/授权日:2018-07-19
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