Invention Grant
- Patent Title: Sequencing device
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Application No.: US16289508Application Date: 2019-02-28
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Publication No.: US10894982B2Publication Date: 2021-01-19
- Inventor: Jonathan Schultz , Todd Roswech , Jon A. Hoshizaki , Albert L. Carrillo , James A. Ball
- Applicant: LIFE TECHNOLOGIES CORPORATION
- Applicant Address: US CA Carlsbad
- Assignee: LIFE TECHNOLOGIES CORPORATION
- Current Assignee: LIFE TECHNOLOGIES CORPORATION
- Current Assignee Address: US CA Carlsbad
- Main IPC: C12Q1/6869
- IPC: C12Q1/6869 ; C12Q1/6806 ; B01L3/00 ; G01N27/414 ; G01N35/10 ; B01F5/06 ; B01F11/00 ; B01F1/00

Abstract:
A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.
Public/Granted literature
- US20190264274A1 SEQUENCING DEVICE Public/Granted day:2019-08-29
Information query