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公开(公告)号：US12050226B2
公开(公告)日：2024-07-30
申请号：US17465303
申请日：2021-09-02
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： James A. Ball , Jonathan Schultz
IPC: B01J19/00 , C12Q1/6869 , G01N33/84
CPC classification number: G01N33/84 , B01J19/0086 , C12Q1/6869 , C12Q1/6869 , C12Q2527/119 , C12Q2527/125 , C12Q2565/607
Abstract: A buffered suspension includes a surfactant and a solid buffer particulate having a point of zero charge at least 1.2 pH units different that the pH of the buffered suspension. The buffered suspension can be prepared by mixing a stock solution with the solid buffer particulate and titrating. A method of preforming a pH sensitive process includes drawing the buffered suspension from a reservoir, filtering the solid buffer particulate from the buffered suspension, and applying the filtered solution to a sensor.
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公开(公告)号：US11987842B2
公开(公告)日：2024-05-21
申请号：US17146376
申请日：2021-01-11
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan Schultz , Todd Roswech , Jon A. Hoshizaki , Albert L. Carrillo , James A Ball
IPC: C12Q1/6869 , B01F1/00 , B01F11/00 , B01F21/20 , B01F25/451 , B01F31/31 , B01F31/65 , B01L3/00 , C12Q1/6806 , G01N27/414 , G01N35/10
CPC classification number: C12Q1/6869 , B01F21/22 , B01F25/451 , B01F31/311 , B01F31/65 , B01L3/502 , B01L3/527 , C12Q1/6806 , G01N27/4145 , G01N35/1002 , G01N35/1095 , B01L3/505 , B01L3/563 , B01L2200/04 , B01L2200/16 , B01L2300/022 , B01L2300/0681 , B01L2300/0861 , B01L2300/123 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0683 , Y10T436/11 , Y10T436/143333
Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.
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公开(公告)号：US20230044160A1
公开(公告)日：2023-02-09
申请号：US17970202
申请日：2022-10-20
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan Schultz , Todd Roswech
Abstract: An apparatus for preparing a reagent solution includes an enclosure and a container disposed within the enclosure. The container defines an internal cavity having a compressible volume and defines a passage providing fluidic communication between the internal cavity and the exterior of the container. Optionally, a compressible member is disposed within the internal cavity. A reagent is disposed within the internal cavity.
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公开(公告)号：US11040344B2
公开(公告)日：2021-06-22
申请号：US16687672
申请日：2019-11-18
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan Schultz , David Marran
IPC: B01L3/00 , C12Q1/6869 , G01N27/447 , G01N35/10
Abstract: The invention provides a passive fluidics circuit for directing different fluids to a common volume, such as a reaction chamber or flow cell, without intermixing or cross contamination. The direction and rate of flow through junctions, nodes and passages of the fluidics circuit are controlled by the states of upstream valves (e.g. opened or closed), differential fluid pressures at circuit inlets or upstream reservoirs, flow path resistances, and the like. Free diffusion or leakage of fluids from unselected inlets into the common outlet or other inlets at junctions or nodes is prevented by the flow of the selected inlet fluid, a portion of which sweeps by the inlets of unselected fluids and exits the fluidics circuit by waste ports, thereby creating a barrier against undesired intermixing with the outlet flow through leakage or diffusion.
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公开(公告)号：US10934155B2
公开(公告)日：2021-03-02
申请号：US16587678
申请日：2019-09-30
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Todd Roswech , Jonathan Schultz , Chun Ho
Abstract: A fluidic interconnect includes a first interface including a liquid port, a gas port, and a cradle; a second interface including a liquid port, a gas port, and a swing bar to engage the cradle, a weight of a container attached to one of the first or second interfaces to drive the liquid port of the first interface into connection with the liquid port of the second interface and the gas port of the first interface into connection with the gas port of the second interface.
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Patent Agency Ranking
公开(公告)号：US20200024659A1
公开(公告)日：2020-01-23
申请号：US16535565
申请日：2019-08-08
Applicant: Life Technologies Corporation
Inventor： Jonathan Schultz , John Davidson
IPC: C12Q1/6869 , C12Q1/6874
Abstract: A method of sequencing a polynucleotide strand can include providing the polynucleotide strand with a primer annealed thereto and a polymerase operably bound to the polynucleotide strand; successively exposing the polynucleotide strand to the flow of four different dNTPs according to a first predetermined ordering; and successively exposing the polynucleotide strand to the flow of four different dNTPs according to a second predetermined ordering, wherein the second predetermined ordering is different from the first predetermined ordering.
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公开(公告)号：US10378663B2
公开(公告)日：2019-08-13
申请号：US16148992
申请日：2018-10-01
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan Schultz
Abstract: A valve for regulating fluid flow includes a housing base defining a lower cavity and comprising a pinch structure within the lower cavity, a gas inlet providing external access to the lower cavity, a base fluid inlet, and a base fluid outlet. A housing cover defines an upper cavity and comprises a cover fluid inlet and a cover fluid outlet. The cover fluid inlet is in fluidic communication with the base fluid outlet between the upper cavity and the lower cavity, and the cover fluid outlet provides external access from the upper cavity. A diaphragm is disposed between the housing base and the housing cover. A pinch plate is disposed in the lower cavity and comprises a pinch point disposed opposite the pinch structure. A pinch tube is in fluidic communication between the base fluid inlet and the base fluid outlet in the lower cavity.
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公开(公告)号：US10100357B2
公开(公告)日：2018-10-16
申请号：US15043296
申请日：2016-02-12
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Todd Rearick , Mark James Milgrew , Jonathan Schultz , Chris Papalias , Kim L. Johnson
IPC: C12Q1/68 , G06F19/22 , C12Q1/6874
Abstract: In one implementation, a method is described. The method includes determining an operational characteristic of sensors of a sensor array. The method further includes selecting a group of sensors in the array based on the operational characteristic of sensors in the group. The method further includes enabling readout of the sensors in the selected group. The method further includes receiving output signals from the enabled sensors, the output signals indicating chemical reactions occurring proximate to the sensors of the sensor array.
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公开(公告)号：US09964515B2
公开(公告)日：2018-05-08
申请号：US14862930
申请日：2015-09-23
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan M. Rothberg , James Bustillo , Mark James Milgrew , Jonathan Schultz , David Marran , Todd Rearick , Kim L. Johnson
IPC: G01N27/403 , G01N27/414 , C12Q1/68 , H01L21/82
CPC classification number: G01N27/4145 , C12Q1/6869 , C12Q1/6874 , G01N27/4148 , H01L21/82 , C12Q2565/607
Abstract: An apparatus comprising a chemical field effect transistor array in a circuit-supporting substrate is disclosed. The transistor array has disposed on its surface an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid. The transistor array has a pitch of 10 μm or less and a sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region.
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公开(公告)号：US11951474B2
公开(公告)日：2024-04-09
申请号：US17304452
申请日：2021-06-21
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan Schultz , David Marran
IPC: C12Q1/6869 , B01L3/00 , G01N27/447 , G01N35/10
CPC classification number: B01L3/502715 , B01L3/502 , B01L3/50273 , B01L3/502738 , B01L3/502746 , B01L3/502776 , B01L3/52 , C12Q1/6869 , G01N27/447 , B01J2219/00389 , B01J2219/00394 , B01L3/502769 , B01L2200/16 , B01L2300/0636 , B01L2300/0645 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/0877 , B01L2400/0487 , G01N35/1002 , G01N35/1097 , Y10T137/0318 , Y10T137/0424 , Y10T137/85938
Abstract: The invention provides a passive fluidics circuit for directing different fluids to a common volume, such as a reaction chamber or flow cell, without intermixing or cross contamination. The direction and rate of flow through junctions, nodes and passages of the fluidics circuit are controlled by the states of upstream valves (e.g. opened or closed), differential fluid pressures at circuit inlets or upstream reservoirs, flow path resistances, and the like. Free diffusion or leakage of fluids from unselected inlets into the common outlet or other inlets at junctions or nodes is prevented by the flow of the selected inlet fluid, a portion of which sweeps by the inlets of unselected fluids and exits the fluidics circuit by waste ports, thereby creating a barrier against undesired intermixing with the outlet flow through leakage or diffusion.
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