- 专利标题: Bead emulsion nucleic acid amplification
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申请号: US16249681申请日: 2019-01-16
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公开(公告)号: US10982274B2公开(公告)日: 2021-04-20
- 发明人: Jan Berka , Yi-Ju Chen , John H. Leamon , Steven Lefkowitz , Kenton L. Lohman , Vinod B. Makhijani , Jonathan M. Rothberg , Gary J. Sarkis , Maithreyan Srinivasan , Michael P. Weiner
- 申请人: Roche Molecular Systems, Inc.
- 申请人地址: US CA Pleasanton
- 专利权人: Roche Molecular Systems, Inc.
- 当前专利权人: Roche Molecular Systems, Inc.
- 当前专利权人地址: US CA Pleasanton
- 代理机构: Cooley LLP
- 代理商 Ivor R. Elrifi; Matthew Pavao
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; B01L3/00 ; C07H21/00 ; C12N15/10 ; G01N21/25 ; G01N21/64 ; G01R33/12 ; C12Q1/686 ; C12Q1/6834 ; C12Q1/6844 ; C12Q1/6865 ; C12Q1/6867 ; C12Q1/6869 ; C12Q1/6874 ; C12Q1/6806 ; B01L7/00
摘要:
Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
公开/授权文献
- US20190203263A1 BEAD EMULSION NUCLEIC ACID AMPLIFICATION 公开/授权日:2019-07-04
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