Invention Application
- Patent Title: PHA-PRODUCING MICROORGANISM HAVING SUCROSE ASSIMILABILITY, AND METHOD FOR PRODUCING PHA USING SAID MICROORGANISM
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Application No.: US16061923Application Date: 2016-12-14
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Publication No.: US20180371509A1Publication Date: 2018-12-27
- Inventor: Hisashi ARIKAWA , Tetsuya FUJIKI
- Applicant: KANEKA CORPORATION
- Applicant Address: JP Osaka-shi, Osaka
- Assignee: KANEKA CORPORATION
- Current Assignee: KANEKA CORPORATION
- Current Assignee Address: JP Osaka-shi, Osaka
- Priority: JP2015-245214 20151216
- International Application: PCT/JP2016/087292 WO 20161214
- Main IPC: C12P7/62
- IPC: C12P7/62 ; C08G63/06 ; C12N9/10 ; C12N15/52 ; C12N1/20

Abstract:
An object of the present invention is to provide a PHA-producing microorganism which can assimilate sucrose, and a method for producing a PHA by culturing this microorganism, using sucrose as a carbon source. A PHA-producing microorganism, comprising a PHA synthase gene, and heterogeneous-organism-derived genes in the following items (1) and (2): (1) a sucrose hydrolase gene encoding an amino acid sequence described in SEQ ID NO: 1, or a gene encoding a polypeptide which has a sequence homology of 90% or more to the amino acid sequence and which has sucrose hydrolase activity; and (2) a sucrose permease gene encoding an amino acid sequence described in SEQ ID NO: 2, or a gene encoding a polypeptide which has a sequence homology of 90% or more to the amino acid sequence and which has sucrose permease activity. The invention is also a method for producing a PHA, including the step of culturing this microorganism in a medium including sucrose as a carbon source.
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