公开(公告)号：US20180371509A1

公开(公告)日：2018-12-27

申请号：US16061923

申请日：2016-12-14

Applicant: KANEKA CORPORATION

Inventor： Hisashi ARIKAWA ,  Tetsuya FUJIKI

IPC: C12P7/62 ,  C08G63/06 ,  C12N9/10 ,  C12N15/52 ,  C12N1/20

Abstract: An object of the present invention is to provide a PHA-producing microorganism which can assimilate sucrose, and a method for producing a PHA by culturing this microorganism, using sucrose as a carbon source. A PHA-producing microorganism, comprising a PHA synthase gene, and heterogeneous-organism-derived genes in the following items (1) and (2): (1) a sucrose hydrolase gene encoding an amino acid sequence described in SEQ ID NO: 1, or a gene encoding a polypeptide which has a sequence homology of 90% or more to the amino acid sequence and which has sucrose hydrolase activity; and (2) a sucrose permease gene encoding an amino acid sequence described in SEQ ID NO: 2, or a gene encoding a polypeptide which has a sequence homology of 90% or more to the amino acid sequence and which has sucrose permease activity. The invention is also a method for producing a PHA, including the step of culturing this microorganism in a medium including sucrose as a carbon source.

公开(公告)号：US20180346943A1

公开(公告)日：2018-12-06

申请号：US16100688

申请日：2018-08-10

Applicant: KANEKA CORPORATION

Inventor： Shingo KOBAYASHI ,  Tetsuya FUJIKI

IPC: C12P7/62 ,  C08G63/06 ,  C08G63/91

Abstract: The present invention provides a novel polyhydroxyalkanoic acid having a functional group at a terminal carboxy group, which being capable of being chemically modified and easily controlled in reaction, and a method for production the same. The present polyhydroxyalkanoic acid includes a specific alkyne group, alkene group, thiol group, azide group or allyl group introduced at a terminal carboxy group. Further, the present production method comprises culturing a microorganism capable of producing a polyhydroxyalkanoic acid with use of an alcohol having an alkyne group, an alkene group, a thiol group, an azide group or an allyl group.

公开(公告)号：US20180305722A1

公开(公告)日：2018-10-25

申请号：US15763323

申请日：2016-09-15

Applicant: KANEKA CORPORATION

Inventor： Shingo KOBAYASHI ,  Hitoshi SASHIWA ,  Tetsuya FUJIKI

IPC: C12P7/62 ,  C08G63/06 ,  C12N15/74 ,  C12N1/20

CPC classification number: C12P7/625 ,  C08G63/06 ,  C12N1/20 ,  C12N15/09 ,  C12N15/74 ,  C12P7/62

Abstract: An object of the invention is to improve the crystallization speed of a PHA copolymer which is to be slowly crystallized, and improve the melt workability and productivity. A microorganism is used which has genes encoding two or more different PHA synthases derived from the genus Aeromonas. The genes encoding the PHA synthases derived from the genus Aeromonas preferably include genes encoding at least two PHA synthases which are capable of synthesizing a copolymer PHA including, as monomer unit species, 3-hydroxybutyric acid and 3-hydroxyhexanoic acid, and which are different in substrate specificity toward 3-hydroxyhexanoic acid from each other. When this microorganism is cultured, a PHA mixture can be produced which includes three or more PHA species different in melting point from each other.