发明授权
- 专利标题： Cell culture medium for human liver epithelial cell line
- 专利标题（中）： 人肝上皮细胞系细胞培养液
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申请号： US844873申请日： 1992-03-03
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公开(公告)号： US5342777A公开(公告)日： 1994-08-30
- 发明人: Katharine H. Cole , John F. Lechner , Curtis C. Harris
- 申请人： Katharine H. Cole , John F. Lechner , Curtis C. Harris
- 申请人地址： DC Washington
- 专利权人： The United States of America as represented by the Secretary of the Dept. of Health and Human Services
- 当前专利权人： The United States of America as represented by the Secretary of the Dept. of Health and Human Services
- 当前专利权人地址： DC Washington
- 主分类号： C12N5/071
- IPC分类号： C12N5/071 ; C12Q1/04 ; G01N33/50 ; C12N1/00 ; C12N5/00 ; C12N15/00 ; C12Q1/00
摘要：
The present invention relates to cell medium developed to support long term multiplication and permanent establishment of a cell line of human liver epithelial cells. The medium may contain an effective cell growth promoting amount of calcium ions; an effective cell growth promoting amount of glucose; an effective amount of insulin to aid cells in glucose uptake; an effective cell growth promoting amount of hydrocortisone; an effective amount of epidermal growth factor to bind epidermal growth factor receptors on cells; an effective amount of transferrin to increase DNA synthesis in cells; an effective amount of cholera toxin to increase DNA synthesis in cells; an effective amount of triiodothyronine to increase DNA synthesis in cells; and an effective growth promoting amount of mammalian hormones and mitogenic factors, including lipoprotein, cholesterol, phospholipids and fatty acids.
公开/授权文献
- US5991063A Optical scanning device 公开/授权日：1999-11-23
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