公开(公告)号：US08513214B2

公开(公告)日：2013-08-20

申请号：US12741873

申请日：2008-11-10

申请人： Bao-Han C. Vu ,  Maqbool A. Siddiqui ,  Victor E. Marquez ,  Stephen H. Hughes ,  Paul L. Boyer

发明人： Bao-Han C. Vu ,  Maqbool A. Siddiqui ,  Victor E. Marquez ,  Stephen H. Hughes ,  Paul L. Boyer

IPC分类号： A01N43/04 ,  A61K31/70 ,  C07H19/16 ,  C07H19/167 ,  C07H19/173 ,  C12Q1/70

CPC分类号： C07D473/34 ,  C07H19/16

摘要： The invention provides for novel 2-Deoxyadenosine compounds, which can treat HIV infection at low cytotoxicity values. Substitution at the 4′-position provided compounds which demonstrated low cytotoxicity values in an ATP-based cytotoxicity assay.

摘要翻译： 本发明提供新的2-脱氧腺苷化合物，其可以以低细胞毒性值治疗HIV感染。 在4'-位上的取代提供了在基于ATP的细胞毒性测定中证实低细胞毒性值的化合物。

公开(公告)号：US09125923B2

公开(公告)日：2015-09-08

申请号：US12997419

申请日：2009-06-11

申请人： Xin W. Wang ,  Junfang Ji ,  Carlo M. Croce ,  Hui-chuan Sun ,  Zhao-you Tang

发明人： Xin W. Wang ,  Junfang Ji ,  Carlo M. Croce ,  Hui-chuan Sun ,  Zhao-you Tang

IPC分类号： C40B30/04 ,  A61K31/70 ,  C12Q1/68

CPC分类号： A61K31/70 ,  A61K38/212 ,  C12Q1/6809 ,  C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/136 ,  C12Q2600/178 ,  C12Q2525/207

摘要： It is disclosed herein that expression of microRNA-26 is decreased in hepatocellular (HCC) tumor tissue relative to non-cancerous tissue, and that a low level of microRNA-26 is associated with a poor clinical outcome. It is also disclosed herein that a low expression level of microRNA-26 is correlated with a favorable response to interferon (IFN)-α therapy in HCC patients. Thus, provided herein is a method of predicting the clinical outcome of a patient diagnosed with HCC comprising detecting the level of microRNA-26 expression in a sample obtained from the patient. Also provided is a method of selecting a patient diagnosed with HCC as a candidate for IFN-α therapy, comprising detecting the level of microRNA-26 expression in a sample obtained from the patient. A method of identifying therapeutic agents for the treatment of HCC, comprising screening candidate agents in vitro to select an agent that increases expression of microRNA-26 in HCC cells are also provided. Further provided are methods of treating a patient diagnosed with HCC and expressing a low level of miR-26, wherein treatment comprises IFN-α therapy.

摘要翻译： 本文公开了相对于非癌组织，肝细胞（HCC）肿瘤组织中microRNA-26的表达降低，并且低水平的microRNA-26与不良临床结果相关。 本文还公开了低表达水平的微小RNA-26与HCC患者中对干扰素（IFN）-α治疗的有利反应相关。 因此，本文提供了一种预测诊断为HCC的患者的临床结果的方法，包括检测从患者获得的样品中的microRNA-26表达水平。 还提供了选择被诊断为HCC的患者作为IFN-α治疗的候选者的方法，包括检测从患者获得的样品中的microRNA-26表达水平。 还提供了鉴定用于治疗HCC的治疗剂的方法，其包括在体外筛选候选试剂以选择增加HCC细胞中微小RNA-26表达的试剂。 还提供了治疗诊断为HCC并表达低水平的miR-26的患者的方法，其中治疗包括IFN-α治疗。

公开(公告)号：US5096733A

公开(公告)日：1992-03-17

申请号：US429033

申请日：1989-10-30

申请人： Val Vallyathan ,  Vincent Castranova ,  Nar S. Dalal ,  Knox Van Dyke

发明人： Val Vallyathan ,  Vincent Castranova ,  Nar S. Dalal ,  Knox Van Dyke

IPC分类号： B24C11/00 ,  C04B20/10

CPC分类号： B82Y30/00 ,  B24C11/00 ,  C04B20/1051 ,  Y10T428/2995

摘要： Cytotoxic effects associated with ground fractured silica-containing minerals and silicates, including asbestos, are prevented by coating the silica-containing minerals and silicates with an aqueous solution containing an aqueously compatible silane coupling agent so that a monomolecular film is formed on the surface of the silica-containing minerals and silicates.

摘要翻译： 通过用含有高度相容的硅烷偶联剂的水溶液涂覆含二氧化硅的矿物质和硅酸盐，可以防止与地层破碎的含二氧化硅的矿物质和硅酸盐（包括石棉）相关的细胞毒性作用，使得单分子膜形成在 含二氧化硅的矿物和硅酸盐。

公开(公告)号：US10898565B2

公开(公告)日：2021-01-26

申请号：US15996275

申请日：2018-06-01

申请人： Takeda Vaccines, Inc ,  THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE DEPT OF HEALTH AND HUMAN SERVICES

发明人： Jill A. Livengood ,  Claire Kinney ,  Timothy D. Powell ,  Dan T. Stinchcomb ,  Jorge Osorio

IPC分类号： A61K39/12 ,  C12N7/00 ,  A61K39/00

摘要： Embodiments herein report compositions, methods and uses for dengue-4 (DENV-4) virus constructs. Some embodiments concern a composition that includes, but is not limited to, DENV-4 virus constructs alone or in combination with other constructs, can be used in a vaccine composition to induce an immune response in a subject. In certain embodiments, compositions can include constructs of more than one serotypes of dengue virus, such as dengue-1 virus, dengue-2 virus, or dengue-3 virus in combination with DENV-4 virus constructs disclosed herein. In other embodiments, DENV-4 constructs disclosed herein can be combined in a composition with other flavivirus constructs to generate a vaccine against more than one flavivirus. Other embodiments provide methods and uses for DENV-4 virus constructs in vaccine compositions that when administered to a subject induce an immune response in the subject against DENV-4 that is improved by modified constructs compared to other vaccine compositions.

公开(公告)号：US5378723A

公开(公告)日：1995-01-03

申请号：US182301

申请日：1994-01-18

申请人： Arnold Brossi ,  Xiao-shu He ,  Nigel H. Greig

发明人： Arnold Brossi ,  Xiao-shu He ,  Nigel H. Greig

IPC分类号： A61K31/40 ,  A61K31/407 ,  A61P21/00 ,  A61P21/04 ,  A61P25/02 ,  A61P25/28 ,  A61P27/02 ,  A61P27/06 ,  A61P43/00 ,  C07D495/04 ,  C07D487/00

CPC分类号： C07D495/04

摘要： Substituted carbamates of tricyclic compounds which have a cyclic sulfer atom, provide highly potent and selective cholinergic agonist and blocking activity are useful as pharmaceutical agents. Cholinergic disease are treated with these compounds such as glaucoma, Myasthenia Gravis, Alzheimer's disease. Methods for inhibiting esterases, acetylcholinesterase and butyrylcholinesterase are also provided.

摘要翻译： 具有环状硫原子的三环化合物的取代的氨基甲酸酯可提供高效和选择性的胆碱能激动剂和阻断活性，可用作药剂。 胆碱能疾病用这些化合物治疗，如青光眼，重症肌无力，阿尔茨海默病。 还提供了抑制酯酶，乙酰胆碱酯酶和丁酰胆碱酯酶的方法。

公开(公告)号：US5342777A

公开(公告)日：1994-08-30

申请号：US844873

申请日：1992-03-03

申请人： Katharine H. Cole ,  John F. Lechner ,  Curtis C. Harris

发明人： Katharine H. Cole ,  John F. Lechner ,  Curtis C. Harris

IPC分类号： C12N5/071 ,  C12Q1/04 ,  G01N33/50 ,  C12N1/00 ,  C12N5/00 ,  C12N15/00 ,  C12Q1/00

CPC分类号： G01N33/5017 ,  C12N5/067 ,  C12Q1/045 ,  C12N2500/14 ,  C12N2500/20 ,  C12N2500/22 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/395 ,  C12N2510/04 ,  G01N2333/914 ,  Y10S435/948

摘要： The present invention relates to cell medium developed to support long term multiplication and permanent establishment of a cell line of human liver epithelial cells. The medium may contain an effective cell growth promoting amount of calcium ions; an effective cell growth promoting amount of glucose; an effective amount of insulin to aid cells in glucose uptake; an effective cell growth promoting amount of hydrocortisone; an effective amount of epidermal growth factor to bind epidermal growth factor receptors on cells; an effective amount of transferrin to increase DNA synthesis in cells; an effective amount of cholera toxin to increase DNA synthesis in cells; an effective amount of triiodothyronine to increase DNA synthesis in cells; and an effective growth promoting amount of mammalian hormones and mitogenic factors, including lipoprotein, cholesterol, phospholipids and fatty acids.

摘要翻译： 本发明涉及开发用于支持长期繁殖和永久建立人肝上皮细胞细胞系的细胞培养基。 介质可能含有有效的细胞生长促进量的钙离子; 有效的细胞生长促进量的葡萄糖; 有效量的胰岛素以帮助细胞摄取葡萄糖; 有效的细胞生长促进量的氢化可的松; 有效量的表皮生长因子结合细胞上的表皮生长因子受体; 有效量的转铁蛋白增加细胞中的DNA合成; 有效量的霍乱毒素以增加细胞中的DNA合成; 有效量的三碘甲状腺原氨酸增加细胞中的DNA合成; 以及哺乳动物激素和促有丝分裂因子的有效生长促进量，包括脂蛋白，胆固醇，磷脂和脂肪酸。