发明授权
- 专利标题: Three-dimensional stromal tissue cultures
- 专利标题(中): 三维基质组织培养
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申请号: US157306申请日: 1998-09-18
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公开(公告)号: US5962325A公开(公告)日: 1999-10-05
- 发明人: Gail K. Naughton , Brian A. Naughton
- 申请人: Gail K. Naughton , Brian A. Naughton
- 申请人地址: CA La Jolla
- 专利权人: Advanced Tissue Sciences, Inc.
- 当前专利权人: Advanced Tissue Sciences, Inc.
- 当前专利权人地址: CA La Jolla
- 主分类号: C12Q1/02
- IPC分类号: C12Q1/02 ; A61B10/00 ; A61B10/02 ; A61B19/00 ; A61F2/00 ; A61F2/04 ; A61F2/06 ; A61F2/08 ; A61K35/12 ; A61K35/28 ; A61L27/00 ; A61L27/38 ; A61L27/60 ; C12N5/00 ; C12N5/071 ; C12N5/077 ; C12N5/0789 ; G01N33/50 ; A01N1/02 ; A61F2/10
摘要:
The present invention relates to a method of stimulating the proliferation and appropriate cell maturation of a variety of different cells and tissues in three-dimensional cultures in vitro using TGF-.beta. in the culture medium. In accordance with the invention, stromal cells, including, but not limited to, chondrocytes, chondrocyte-progenitors, fibroblasts, fibroblast-like cells, umbilical cord cells or bone marrow cells from umbilical cord blood are inoculated and grown on a three-dimensional framework in the presence of TGF-.beta.. Stromal cells may also include other cells found in loose connective tissue such as endothelial cells, macrophages/monocytes, adipocytes, pericytes, reticular cells found in bone marrow stroma, etc. The stromal cells and connective tissue proteins naturally secreted by the stromal cells attach to and substantially envelope the framework composed of a biocompatible non-living material formed into a three-dimensional structure having interstitial spaces bridged by the stromal cells. The living stromal tissue so formed provides the support, growth factors, and regulatory factors necessary to sustain long-term active proliferation of cells in culture and/or cultures implanted in vivo. When grown in this three-dimensional system, the proliferating cells mature and segregate properly to form components of adult tissues analogous to counterparts in vivo.
公开/授权文献
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