发明授权
US07732179B2 Mutants of pyrroloquinoline quinone dependent soluble glucose dehydrogenase
有权
吡咯喹啉醌依赖性可溶性葡萄糖脱氢酶的突变体
- 专利标题: Mutants of pyrroloquinoline quinone dependent soluble glucose dehydrogenase
- 专利标题(中): 吡咯喹啉醌依赖性可溶性葡萄糖脱氢酶的突变体
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申请号: US12250241申请日: 2008-10-13
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公开(公告)号: US07732179B2公开(公告)日: 2010-06-08
- 发明人: Mara Boenitz-Dulat , Daniela Beck , Peter Kratzsch , Rainer Schmuck , Herbert Von Der Eltz
- 申请人: Mara Boenitz-Dulat , Daniela Beck , Peter Kratzsch , Rainer Schmuck , Herbert Von Der Eltz
- 申请人地址: US IN Indianapolis
- 专利权人: Roche Diagnostics Operations, Inc.
- 当前专利权人: Roche Diagnostics Operations, Inc.
- 当前专利权人地址: US IN Indianapolis
- 代理机构: Barnes & Thornburg LLP
- 优先权: EP06007779 20060413; WOPCT/EP2007/003207 20070411
- 主分类号: C12N9/04
- IPC分类号: C12N9/04 ; C12N15/00 ; C12N1/20 ; C12Q1/32 ; C12Q1/00 ; C12P21/04 ; C07H21/04 ; C12Q1/68
摘要:
A mutant of PQQ-dependent soluble glucose dehydrogenase (s-GDH; EC 1.1.5.2) is provided with improved specificity for glucose as compared to maltose, having a substitution of threonine at position 348 by either glycine, alamine or serine, wherein said mutant additionally comprises, at least one mutation for improving the stability of the mutant and one or more mutation(s) for improving the affinity of the mutant to glucose, and/or one or more mutation(s) for further improving the specificity of the mutant for glucose as compared to maltose, and wherein position 348 correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence. Also disclosed are genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.