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    Mutants of pyrroloquinoline quinone dependent soluble glucose dehydrogenase
    1.
    发明授权
    Mutants of pyrroloquinoline quinone dependent soluble glucose dehydrogenase 有权
    吡咯喹啉醌依赖性可溶性葡萄糖脱氢酶的突变体

    公开(公告)号：US07732179B2

    公开(公告)日：2010-06-08

    申请号：US12250241

    申请日：2008-10-13

    申请人： Mara Boenitz-Dulat ,  Daniela Beck ,  Peter Kratzsch ,  Rainer Schmuck ,  Herbert Von Der Eltz

    发明人： Mara Boenitz-Dulat ,  Daniela Beck ,  Peter Kratzsch ,  Rainer Schmuck ,  Herbert Von Der Eltz

    IPC分类号： C12N9/04 ,  C12N15/00 ,  C12N1/20 ,  C12Q1/32 ,  C12Q1/00 ,  C12P21/04 ,  C07H21/04 ,  C12Q1/68

    CPC分类号： C12N9/0006

    摘要： A mutant of PQQ-dependent soluble glucose dehydrogenase (s-GDH; EC 1.1.5.2) is provided with improved specificity for glucose as compared to maltose, having a substitution of threonine at position 348 by either glycine, alamine or serine, wherein said mutant additionally comprises, at least one mutation for improving the stability of the mutant and one or more mutation(s) for improving the affinity of the mutant to glucose, and/or one or more mutation(s) for further improving the specificity of the mutant for glucose as compared to maltose, and wherein position 348 correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence. Also disclosed are genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.

    摘要翻译： 与麦芽糖相比，PQQ依赖性可溶性葡萄糖脱氢酶（s-GDH; EC 1.1.5.2）的突变体具有改善的葡萄糖特异性，其具有通过甘氨酸，丙氨酸或丝氨酸取代位置348处的苏氨酸，其中所述突变体 还包含至少一个用于提高突变体的稳定性的突变和用于提高突变体对葡萄糖的亲和力的一个或多个突变，和/或用于进一步提高突变体的特异性的一个或多个突变 用于与麦芽糖相比的葡萄糖，并且其中位置348对应于从A.calcoaceticus s-GDH野生型序列已知的氨基酸位置。 还公开了编码这种突变体s-GDH的基因以及这些s-GDH突变体的不同应用，特别是用于测定样品中葡萄糖浓度。

    MUTANTS OF PYRROLOQUINOLINE QUINONE DEPENDENT SOLUBLE GLUCOSE DEHYDROGENASE
    2.
    发明申请
    MUTANTS OF PYRROLOQUINOLINE QUINONE DEPENDENT SOLUBLE GLUCOSE DEHYDROGENASE 有权
    吡咯烷醇喹诺酮的可溶性葡萄糖脱氢酶

    公开(公告)号：US20090148874A1

    公开(公告)日：2009-06-11

    申请号：US12250241

    申请日：2008-10-13

    申请人： Mara Boenitz-Dulat ,  Daniela Beck ,  Peter Kratzsch ,  Rainer Schmuck ,  Herbert Von Der Eltz

    发明人： Mara Boenitz-Dulat ,  Daniela Beck ,  Peter Kratzsch ,  Rainer Schmuck ,  Herbert Von Der Eltz

    IPC分类号： C12Q1/54 ,  C12N9/04 ,  C12N15/11 ,  C12N15/00 ,  C12M1/00 ,  C12N1/21 ,  C12P21/02

    CPC分类号： C12N9/0006

    摘要： A mutant of PQQ-dependent soluble glucose dehydrogenase (s-GDH; EC 1.1.5.2) is provided with improved specificity for glucose as compared to maltose, having a substitution of threonine at position 348 by either glycine, alamine or serine, wherein said mutant additionally comprises, at least one mutation for improving the stability of the mutant and one or more mutation(s) for improving the affinity of the mutant to glucose, and/or one or more mutation(s) for further improving the specificity of the mutant for glucose as compared to maltose, and wherein position 348 correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence. Also disclosed are genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.

    摘要翻译： 与麦芽糖相比，PQQ依赖性可溶性葡萄糖脱氢酶（s-GDH; EC 1.1.5.2）的突变体具有改善的葡萄糖特异性，其具有通过甘氨酸，丙氨酸或丝氨酸取代位置348处的苏氨酸，其中所述突变体 还包含至少一个用于提高突变体的稳定性的突变和用于提高突变体对葡萄糖的亲和力的一个或多个突变，和/或用于进一步提高突变体的特异性的一个或多个突变 用于与麦芽糖相比的葡萄糖，并且其中位置348对应于从A.calcoaceticus s-GDH野生型序列已知的氨基酸位置。 还公开了编码这种突变体s-GDH的基因以及这些s-GDH突变体的不同应用，特别是用于测定样品中葡萄糖浓度。

    Nucleotides labelled with an infrared dye and their use in nucleic acid detection
    4.
    发明授权
    Nucleotides labelled with an infrared dye and their use in nucleic acid detection 失效
    用红外染料标记的核苷酸及其在核酸检测中的用途

    公开(公告)号：US06573374B1

    公开(公告)日：2003-06-03

    申请号：US08411761

    申请日：1995-03-28

    申请人： Klaus Muehlegger ,  Hans-Joachim Hoeltke ,  Christian Birkner ,  Herbert Von Der Eltz

    发明人： Klaus Muehlegger ,  Hans-Joachim Hoeltke ,  Christian Birkner ,  Herbert Von Der Eltz

    IPC分类号： C07H1904

    CPC分类号： C07H19/10 ,  C07F9/5728 ,  C07H19/20 ,  C07H21/00

    摘要： Nucleoside-5′-triphosphates and phosphoramidites which carry a residue absorbing in the long wavelength region, preferably a carbocyanine group of the general formula (I), on the base portion or on the phosphorus atom in which R1 and R2 each denote hydrogen or together form a phenyl residue; R3 denotes hydrogen if linkage with the nucleotide is via the R4 position or it denotes a —NHCS— group if linkage with the nucleotide is via the R3 position; both R4 and R5, or R5, alone denote an alkylsulfonyl group with n being a number from 3 to 5 or R4 represents a —NHCS— group with n being a number from 3 to 8, as well as the use of the compounds to label, detect and sequence nucleic acids.

    摘要翻译： 核苷-5'-三磷酸和亚磷酰胺，其在碱性部分或磷原子上具有在长波长区域吸收的残基，优选为通式（I）的碳菁基团，其中R1和R2各自表示氢或一起形成 苯基残基; 如果与核苷酸的连接通过R4位置，R3表示氢，如果与核苷酸的连接通过R3位置，则表示-NHCS-基团; R4和R5或R5独立地表示烷基磺酰基，n为3至5的数，或R4表示-NHCS-基，n为3至8的数，以及该化合物用于标记 ，检测和序列核酸。

    Nucleosides, nucleotides and oligonucleotides containing enzymatically cleavable protecting groups
    8.
    发明授权
    Nucleosides, nucleotides and oligonucleotides containing enzymatically cleavable protecting groups 失效
    含有酶切割保护基团的核苷，核苷酸和寡核苷酸

    公开(公告)号：US5677441A

    公开(公告)日：1997-10-14

    申请号：US326490

    申请日：1994-10-20

    申请人： Herbert Waldman ,  Armin Reidel ,  Axel Heuser ,  Klaus Muehlegger ,  Herbert Von Der Eltz ,  Christian Birkner

    发明人： Herbert Waldman ,  Armin Reidel ,  Axel Heuser ,  Klaus Muehlegger ,  Herbert Von Der Eltz ,  Christian Birkner

    IPC分类号： C12Q1/68 ,  C07H19/04 ,  C07H19/073 ,  C07H19/173 ,  C07H21/00 ,  C07H21/04 ,  C12Q1/70 ,  C07H19/06 ,  C07H19/16

    CPC分类号： C07H21/00 ,  C07H19/04 ,  Y02P20/55

    摘要： Process for the production of oligonucleotides of formula II, in which the exocyclic amino groups of the bases adenine, guanine, cytosine, 7-deazaadenine and 7-deazaguanine carry N-phenylacetyl groups, are used for oligonucleotide synthesis, wherein in a first step a starting nucleotide is bound to a solid carrier, subsequently the desired oligonucleotide is synthesized by stepwise coupling with appropriately activated further monomeric nucleotide building blocks of the general formula I with the above-mentioned meanings, if desired, trivalent phosphorus is oxidized to pentavalent phosphorus during and after the synthesis, the oligonucleotide is cleaved from the carrier and the 5' protecting groups are cleaved off. The phenylacetyl functional groups that protect exocyclic NH.sub.2 groups of the bases can be cleaved off in a mild way with penicillin amidohydrolase (EC 3.5.1.11).

    摘要翻译： 用于生产式II寡核苷酸的方法，其中碱基腺嘌呤，鸟嘌呤，胞嘧啶，7-脱氮鸟嘌呤和7-脱氮鸟嘌呤的环外氨基携带N-苯乙酰基用于寡核苷酸合成，其中在第一步中 起始核苷酸与固体载体结合，随后所需的寡核苷酸通过与适当活化的具有上述含义的通式I的其它单体核苷酸结构单元逐步偶联来合成，如果需要，三价磷被氧化成五价磷， 合成后，将寡核苷酸从载体上切下，5'保护基被切掉。 保护碱基的环外NH2基团的苯乙酰基官能团可以用青霉素酰胺水解酶（EC 3.5.1.11）以温和的方式裂解。

    PROTEASE INHIBITOR CONJUGATES AND ANTIBODIES USEFUL IN IMMUNOASSAY
    10.
    发明申请
    PROTEASE INHIBITOR CONJUGATES AND ANTIBODIES USEFUL IN IMMUNOASSAY 失效
    蛋白酶抑制剂结合物和抗体在免疫调节中有用

    公开(公告)号：US20060211107A1

    公开(公告)日：2006-09-21

    申请号：US11420502

    申请日：2006-05-26

    申请人： Gerald Sigler ,  Raymond Hui ,  Ina Deras ,  Richard Root ,  Erasmus Huber ,  Herbert Von Der Eltz

    发明人： Gerald Sigler ,  Raymond Hui ,  Ina Deras ,  Richard Root ,  Erasmus Huber ,  Herbert Von Der Eltz

    IPC分类号： C12N5/06 ,  C07K16/18 ,  C07D417/02

    CPC分类号： C07K16/38 ,  C07K16/44

    摘要： Activated haptens useful for generating immunogens to HIV protease inhibitors, immunogens useful for producing antibodies to HIV protease inhibitors, and antibodies and labeled conjugates useful in immunoassays for the HIV protease inhibitor saquinavir. The novel haptens feature an activated functionality at the central, non-terminal hydroxyl group. Also described are monoclonal antibodies specific for saquinavir having less than 10% cross-reactivity with lopinavir, nelfinavir, amprenavir, ritonavir, and indinavir, and a murine hybridoma producing said antibodies.

    摘要翻译： 可用于产生HIV蛋白酶抑制剂免疫原的活化的半抗原，可用于产生HIV蛋白酶抑制剂的抗体的免疫原，以及可用于HIV蛋白酶抑制剂沙奎那韦免疫测定的抗体和标记的缀合物。 新型半抗原在中心非末端羟基处具有活化的功能。 还描述了与洛匹那韦，奈非那韦，安仁那韦，利托那韦和茚地那韦具有小于10％交叉反应性的沙奎那韦特异性单克隆抗体，以及产生所述抗体的鼠杂交瘤。