公开(公告)号：EP1453956B8

公开(公告)日：2010-02-10

申请号：EP02792957.9

申请日：2002-12-10

申请人： Baxter Healthcare S.A.

发明人： REITER, Manfred ,  MUNDT, Wolfgang

IPC分类号： C12N7/02 ,  C12N5/00

CPC分类号： C12N7/00 ,  A61K2039/525 ,  C12N2760/16151 ,  Y02A50/388 ,  Y02A50/39 ,  Y02A50/394

摘要： The present invention is directed to a cell culture biomass of adherent cells having increased cell density and microcarrier concentration compared to the respective confluent cell culture.

公开(公告)号：EP1453956B1

公开(公告)日：2009-10-28

申请号：EP02792957.9

申请日：2002-12-10

申请人： Baxter Healthcare S.A.

发明人： REITER, Manfred ,  MUNDT, Wolfgang

IPC分类号： C12N7/02 ,  C12N5/00

CPC分类号： C12N7/00 ,  A61K2039/525 ,  C12N2760/16151 ,  Y02A50/388 ,  Y02A50/39 ,  Y02A50/394

摘要： The present invention is directed to a cell culture biomass of adherent cells having increased cell density and microcarrier concentration compared to the respective confluent cell culture.

公开(公告)号：EP1453535A2

公开(公告)日：2004-09-08

申请号：EP02796598.7

申请日：2002-12-10

申请人： Baxter Healthcare S.A.

发明人： KISTNER, Otfried ,  REITER, Manfred ,  BRUEHMANN, Axel ,  BARRETT, Noel ,  MUNDT, Wolfgang ,  DORNER, Friedrich

IPC分类号： A61K39/12 ,  C12N7/00

CPC分类号： A61K39/12 ,  A61K39/42 ,  A61K2039/5252 ,  A61K2039/55505 ,  C07K16/1081 ,  C12N7/00 ,  C12N2770/36051 ,  C12N2770/36134 ,  C12N2770/36151

摘要： The present invention provides methods of production of a purified enveloped virus antigen. In particular, it provides purified Ross River Virus (RRV) antigens, and vaccines comprising purified, inactived Ross River Virus (RRV) antigen.

摘要翻译： 本发明提供了生产纯化的包膜病毒抗原的方法。 特别是，它提供了纯化的罗斯河病毒（RRV）抗原和疫苗包含纯化的，灭活的罗斯河病毒（RRV）抗原。

公开(公告)号：EP2155864B1

公开(公告)日：2013-07-17

申请号：EP08749278.1

申请日：2008-04-30

申请人： Baxter International Inc. ,  Baxter Healthcare S.A.

发明人： REITER, Manfred ,  GRILLBERGER, Leopold ,  MUNDT, Wolfgang ,  MITTERER, Artur ,  SCHAFHAUSER, Horst

IPC分类号： C12N7/02 ,  C12Q1/70

CPC分类号： C12N7/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/145 ,  C12N2750/14351 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16152 ,  C12N2760/16234 ,  C12N2760/16251 ,  C12N2760/16252

摘要： The present invention provides a method for purification of a virus or virus antigen comprising providing a virus preparation and centrifugation of said virus preparation in a gradient of a sugar established by the addition of two or more buffered sugar layers of different concentration. The method leads to higher yields and reduces unwanted aggregation of the virus or virus antigen by increasing the volume of the peak pool.

公开(公告)号：EP1520008B1

公开(公告)日：2012-09-05

申请号：EP03762657.9

申请日：2003-07-08

申请人： Baxter International Inc. ,  Baxter Healthcare S.A.

发明人： REITER, Manfred ,  MUNDT, Wolfgang ,  GRILLBERGER, Leopold, Dr. ,  KRAUS, Barbara

IPC分类号： C12N5/00 ,  C12N5/07 ,  C12N5/10 ,  C12N7/00 ,  C12P21/00

CPC分类号： C12N5/005 ,  A61K39/12 ,  A61K39/145 ,  A61K39/275 ,  A61K39/285 ,  C12N5/0043 ,  C12N7/00 ,  C12N2500/74 ,  C12N2500/76 ,  C12N2710/24134 ,  C12N2760/16134 ,  C12N2770/36134 ,  Y02A50/388 ,  Y02A50/39 ,  Y02A50/394 ,  Y02A50/396 ,  Y02A50/466

摘要： The present invention relates to a methods for producing a virus or an immunogenic composition comprising a virus or virus antigen using a culture of cells that have been grown in an animal protein free medium comprising soy hydrolysate and yeast hydrolysate.

公开(公告)号：EP2459702A1

公开(公告)日：2012-06-06

申请号：EP10740810.6

申请日：2010-07-30

申请人： Baxter International Inc ,  Baxter Healthcare S.A.

发明人： GRILLBERGER, Leopold ,  SPENGER, Alexandra ,  HASSLACHER, Meinhard ,  GRILLBERGER, Rana ,  REITER, Manfred

IPC分类号： C12N5/00 ,  C12N9/64

CPC分类号： C12N9/6489 ,  C12N5/0682 ,  C12N5/0686 ,  C12N2500/14 ,  C12N2500/22 ,  C12N2500/38 ,  C12N2500/95 ,  C12N2500/99 ,  C12N2510/02 ,  C12Y304/24087

摘要： The present invention provides culture mediums that are useful for the expression of ADAMTS proteins, such as ADAMTS13. Methods for the expression and purification of ADAMTS proteins are also provided. In some embodiments, the mediums and methods of the invention are useful for the expression of ADAMTS proteins having high specific activities. Also provided are ADAMTS, e.g., ADAMTS13, protein compositions with high specific activities, which are expressed and purified according to the methods provided herein.

公开(公告)号：EP1453535B1

公开(公告)日：2007-09-05

申请号：EP02796598.7

申请日：2002-12-10

申请人： Baxter Healthcare S.A.

发明人： KISTNER, Otfried ,  REITER, Manfred ,  BRUEHMANN, Axel ,  BARRETT, Noel ,  MUNDT, Wolfgang ,  DORNER, Friedrich

IPC分类号： A61K39/12 ,  C12N7/00

CPC分类号： A61K39/12 ,  A61K39/42 ,  A61K2039/5252 ,  A61K2039/55505 ,  C07K16/1081 ,  C12N7/00 ,  C12N2770/36051 ,  C12N2770/36134 ,  C12N2770/36151

摘要： The present invention provides methods of production of a purified enveloped virus antigen. In particular, it provides purified Ross River Virus (RRV) antigens, and vaccines comprising purified, inactived Ross River Virus (RRV) antigen.

公开(公告)号：EP2591094A1

公开(公告)日：2013-05-15

申请号：EP11738351.3

申请日：2011-07-08

申请人： Baxter International Inc. ,  Baxter Healthcare S.A.

发明人： GRILLBERGER, Leopold ,  REITER, Manfred ,  FLEISCHANDERL, Daniel ,  BRAMBERGER, Gregor

IPC分类号： C12N5/10 ,  C12N9/48 ,  C07K14/745 ,  A61K38/36

CPC分类号： C07K14/755 ,  A61K9/0019 ,  A61K38/00 ,  A61K38/36 ,  A61K38/37 ,  C12N9/6489 ,  C12N2500/24 ,  C12N2511/00 ,  C12P21/00 ,  C12Y304/24087

摘要： Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS 13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH 4 + ) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.

摘要翻译： 在其它方面，本发明涉及用于产生高分子量vWF，特别是具有高比活性的高度多聚体WF和具有高比活性的ADAMTS 13的细胞培养条件。 本发明的细胞培养条件可以包括例如具有增加的铜浓度的细胞培养基和/或具有低铵（NH 4 +）浓度的细胞培养上清液。 本发明还提供了在细胞培养条件下培养细胞以表达具有高比活性的高分子量vWF和rA13的方法。

公开(公告)号：EP2590998A1

公开(公告)日：2013-05-15

申请号：EP11736502.3

申请日：2011-07-08

申请人： Baxter International Inc. ,  Baxter Healthcare S.A.

发明人： GRILLBERGER, Leopold ,  REITER, Manfred ,  MUNDT, Wolfgang

IPC分类号： C07K14/755 ,  C12N5/00 ,  A61K38/36 ,  C12N5/10

CPC分类号： C07K14/755 ,  A61K9/0019 ,  A61K38/00 ,  A61K38/36 ,  A61K38/37 ,  C12N9/6489 ,  C12N2500/24 ,  C12N2511/00 ,  C12P21/00 ,  C12Y304/24087

摘要： Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH
4
+ ) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.

摘要翻译： 在其它方面，本发明涉及用于产生高分子量vWF，特别是具有高比活性的高度多聚体WF和具有高比活性的ADAMTS 13的细胞培养条件。 本发明的细胞培养条件可以包括例如具有增加的铜浓度的细胞培养基和/或具有低铵（NH 4 +）浓度的细胞培养上清液。 本发明还提供了在细胞培养条件下培养细胞以表达具有高比活性的高分子量vWF和rA13的方法。

公开(公告)号：EP1456228A2

公开(公告)日：2004-09-15

申请号：EP02798324.6

申请日：2002-12-10

申请人： Baxter Healthcare S.A.

发明人： MITTERER, Artur ,  TAUER, Christa ,  REITER, Manfred ,  MUNDT, Wolfgang

IPC分类号： C07K1/22

CPC分类号： C12N9/6427 ,  C07K14/36 ,  C12N7/00 ,  C12N2760/16051 ,  C12N2760/16151 ,  C12N2770/32451

摘要： The present invention provides methods of isolation and purification of Streptomyces griseus trypsin (SGT) from pronase in a single affinity chromatography step and uses of the purified SGT.