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11 条记录 (耗时 0.016 秒)

    Monoclonal antibody against an acidic FGF protein, its production and use
    1.
    发明公开
    Monoclonal antibody against an acidic FGF protein, its production and use 失效
    MonokeparerAntikörpergegen sauren FGF，seine Herstellung und Verwendung。

    公开(公告)号：EP0420081A1

    公开(公告)日：1991-04-03

    申请号：EP90118234.5

    申请日：1990-09-22

    申请人： Takeda Chemical Industries, Ltd.

    发明人： Ichimori, Yuzo ,  Kondo, Koichi ,  Igarashi, Koichi ,  Senoo, Masaharu

    IPC分类号： C12P21/08 ,  C12N5/18 ,  G01N33/577 ,  G01N33/68 ,  C07K3/20

    CPC分类号： C07K14/501 ,  C07K16/22

    摘要： The present invention provides a hybrid cell line producing monoclonal antibody to an acidic fibroblast growth factor (aFGF) protein. The hybridoma is established by fusing spleen cells from immunized mice with myeloma cells. The hybridomas are cultured as clones, and antibodies obtained from the individual clones are tested for their specificity for aFGF protein.
    Antibodies can be obtained from the culture growth medium or from ascitic fluid of mice bearing the hybridoma tumor. Diagnostic and therapeutic uses of the monoclonal antibody are also disclosed.

    摘要翻译： 本发明提供了产生对酸性成纤维细胞生长因子（aFGF）蛋白质的单克隆抗体的杂交细胞系。 通过将来自免疫小鼠的脾细胞与骨髓瘤细胞融合来建立杂交瘤。 将杂交瘤作为克隆进行培养，并测试从各克隆获得的抗体对aFGF蛋白的特异性。 抗体可以从培养生长培养基或携带杂交瘤肿瘤的小鼠的腹水获得。 还公开了单克隆抗体的诊断和治疗用途。

    Polypeptide, DNA and its use
    3.
    发明公开
    Polypeptide, DNA and its use 失效
    多肽，DNA和我的Verwendung。

    公开(公告)号：EP0326907A1

    公开(公告)日：1989-08-09

    申请号：EP89101162.9

    申请日：1989-01-24

    申请人： Takeda Chemical Industries, Ltd.

    发明人： Senoo, Masaharu ,  Sasada, Reiko ,  Kurokawa, Tsutomu ,  Igarashi, Koichi

    IPC分类号： C12N15/12 ,  C12N15/18

    CPC分类号： C07K14/503 ,  A61K38/00 ,  C07K2319/02 ,  C07K2319/75

    摘要： A mutein of basic fibroblast growth factor (bFGF) which lacks 7 to 46 amino acids of the constituent amino acids of bFGF from carboxyl terminal possesses excellent fibroblast growth promoting activities, vasoendothelial cell growth promoting activities and angiogenic activities. It is very stable in living cells. The mutein of the present invention therefore function well as a healing promoter for burns etc., therapeutic drug for thrombosis, arteriosclerosis etc., and cell cultivation promoter.

    摘要翻译： 碱性成纤维细胞生长因子（bFGF）从羧基末端缺乏bFGF组成氨基酸7〜46个氨基酸的突变蛋白具有优异的成纤维细胞生长促进活性，血管内皮细胞生长促进活性和血管生成活性。 它在活细胞中非常稳定。 因此，本发明的突变蛋白功能良好，作为灼伤的治疗促进剂等，用于血栓形成，动脉硬化等的治疗药物和细胞培养促进剂。

    DNA and use thereof
    4.
    发明公开
    DNA and use thereof 失效
    DNA及其用途

    公开(公告)号：EP0158198A1

    公开(公告)日：1985-10-16

    申请号：EP85103450.4

    申请日：1985-03-23

    申请人： Takeda Chemical Industries, Ltd.

    发明人： Senoo, Masaharu ,  Onda, Haruo ,  Igarashi, Koichi

    IPC分类号： C12N15/00 ,  C12P19/34 ,  C12P21/02 ,  C07K7/10 ,  C07K15/26

    CPC分类号： C07K14/55 ,  C07K14/57 ,  C07K2319/00 ,  C07K2319/75

    摘要： A polypeptide comprising amino acid sequence of human interferon and that of human interleukine-2-may be produced by making new DNA by the ligation of DNA containing the structural gene coding for the peptide of human interferon with DNA containing the structural gene coding for human interleukine-2, transforming the host cells with the DNA, and cultivating the transformant.

    摘要翻译： 包含人干扰素的氨基酸序列和人白细胞介素-2的多肽可以通过将含有编码人干扰素肽的结构基因的DNA与含有编码人白细胞介素的结构基因的DNA -2，用DNA转化宿主细胞，并培养转化体。

    Glycosylated bFGF and production thereof
    7.
    发明公开
    Glycosylated bFGF and production thereof 失效
    Glycosyliertes FGF和dessen Herstellung。

    公开(公告)号：EP0394951A1

    公开(公告)日：1990-10-31

    申请号：EP90107737.0

    申请日：1990-04-24

    申请人： Takeda Chemical Industries, Ltd.

    发明人： Senoo, Masaharu ,  Sasada, Reiko ,  Igarashi, Koichi

    IPC分类号： C12N15/16 ,  C12P21/02

    CPC分类号： C07K14/503 ,  C07K2319/036 ,  C07K2319/75 ,  C07K2319/91 ,  C12N15/625

    摘要： Disclosed are (1) a mutein of a fibroblast growth factor (FGF), the DNA having introduced therein at least one nucleotide sequence coding for a glycosylation site, (2) a DNA coding for the mutein of (1), (3) a vector containing the DNA of (2), (4) a transformant transformed with the vector of (3), and (5) a process for producing the mutein which comprises cultivating in a culture medium the transformant of a yeast or animal cell transformed with a vector of (3), and producing and accumulating the mutein of (1) in the culture medium, whereby the FGF mutein into which at least one glycosylation site has been introduced is improved in productivity, stability and activities, and advantageously used as medicine.

    摘要翻译： 公开了（1）成纤维细胞生长因子（FGF）的突变蛋白，其中引入了至少一个编码糖基化位点的核苷酸序列的DNA，（2）编码（1），（3）的突变蛋白的DNA 含有（2）的DNA的载体，（4）用（3）的载体转化的转化体，和（5）生产突变蛋白的方法，其包括在培养基中培养转化了酵母或动物细胞的转化体 （3）的载体，并且在培养基中产生和积累（1）的突变蛋白，从而提高了其中引入了至少一个糖基化位点的FGF突变蛋白的生产力，稳定性和活性，并且有利地用作药物 。