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公开(公告)号：EP0414151B1
公开(公告)日：1995-03-15
申请号：EP90115815.4
申请日：1990-08-17
发明人： Kakinuma, Atsushi , Nakahama, Kazuo , Yoshimura, Koji , Sasada, Reiko , Kaisho, Yoshihiko , Iwane, Makoto
CPC分类号： C07K14/48
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公开(公告)号：EP0394951A1
公开(公告)日：1990-10-31
申请号：EP90107737.0
申请日：1990-04-24
发明人： Senoo, Masaharu , Sasada, Reiko , Igarashi, Koichi
CPC分类号： C07K14/503 , C07K2319/036 , C07K2319/75 , C07K2319/91 , C12N15/625
摘要： Disclosed are (1) a mutein of a fibroblast growth factor (FGF), the DNA having introduced therein at least one nucleotide sequence coding for a glycosylation site, (2) a DNA coding for the mutein of (1), (3) a vector containing the DNA of (2), (4) a transformant transformed with the vector of (3), and (5) a process for producing the mutein which comprises cultivating in a culture medium the transformant of a yeast or animal cell transformed with a vector of (3), and producing and accumulating the mutein of (1) in the culture medium, whereby the FGF mutein into which at least one glycosylation site has been introduced is improved in productivity, stability and activities, and advantageously used as medicine.
摘要翻译： 公开了（1）成纤维细胞生长因子（FGF）的突变蛋白，其中引入了至少一个编码糖基化位点的核苷酸序列的DNA，（2）编码（1），（3）的突变蛋白的DNA 含有（2）的DNA的载体，（4）用（3）的载体转化的转化体，和（5）生产突变蛋白的方法，其包括在培养基中培养转化了酵母或动物细胞的转化体 （3）的载体，并且在培养基中产生和积累（1）的突变蛋白，从而提高了其中引入了至少一个糖基化位点的FGF突变蛋白的生产力，稳定性和活性，并且有利地用作药物 。
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3.发明公开Production of human nerve growth factor proteins 失效Menschliche Nervenwachstumsfaktoren und deren Produktion。
公开(公告)号：EP0414151A1
公开(公告)日：1991-02-27
申请号：EP90115815.4
申请日：1990-08-17
发明人： Kakinuma, Atsushi , Nakahama, Kazuo , Yoshimura, Koji , Sasada, Reiko , Kaisho, Yoshihiko , Iwane, Makoto
CPC分类号： C07K14/48
摘要： Disclosed are (1) an active human NGF protein having 6 cysteine residues in its molecule, in which the first cysteine residue from the N-terminus is bound to the fourth cysteine residue, the second cysteine residue to the fifth cysteine residue, and the third cysteine residue to the sixth cysteine residue to form disulfide bonds, (2) an animal cell which is transformed with a vector containing a gene coding for a human NGF protein and is cloned, (3) the animal cell described in (2), in which a host is a Chinese hamster CHO cell, and (4) a method for producing a human NGF protein which comprises cultivating the animal cell described in (2) or (3) in a medium. The human NGF proteins having specific disulfide bonds can be advantageously used as reagents or therapeutic drugs, and the cloned animal cells can commercially produce the high pure human NGF proteins in large amounts.
摘要翻译： 公开了（1）在其分子中具有6个半胱氨酸残基的活性人NGF蛋白，其中来自N末端的第一半胱氨酸残基与第四半胱氨酸残基，第二半胱氨酸残基连接至第五半胱氨酸残基，第三半胱氨酸残基与第五半胱氨酸残基相连， 半胱氨酸残基至第六半胱氨酸残基以形成二硫键，（2）用含有编码人NGF蛋白的基因的载体转化的动物细胞，并克隆，（3）（2）中描述的动物细胞， 宿主是中国仓鼠CHO细胞，以及（4）生产人NGF蛋白的方法，其包括在培养基中培养（2）或（3）中描述的动物细胞。 具有特异性二硫键的人NGF蛋白质可以有利地用作试剂或治疗药物，并且克隆的动物细胞可以大量商业生产高纯度人NGF蛋白质。
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公开(公告)号：EP0237966A2
公开(公告)日：1987-09-23
申请号：EP87103601.8
申请日：1987-03-12
CPC分类号： C07K14/503
摘要： A human basic fibroblast growth factor (hbFGF), which is usable for a cure promoter for burns etc., therapeutic agent for thrombosis etc. or a reagent for promoting cell cultivation, can be produced in a large quantity by cultivating a transformant as transformed with a vector including a DNA coding the hbFGF.
摘要翻译： 可以通过培养转化的转化体，大量生产可用于烧伤治愈促进剂等的人类碱性成纤维细胞生长因子（hbFGF），血栓形成治疗剂等或促进细胞培养的试剂。 包括编码hbFGF的DNA的载体。
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公开(公告)号：EP0172619A1
公开(公告)日：1986-02-26
申请号：EP85304383.4
申请日：1985-06-19
发明人： Sasada, Reiko , Onda, Haruo , Igarashi, Koichi
CPC分类号： C07K14/55
摘要： This invention relates to animal cells transformed with a DNA having a region coding for a human IL-2 protein and, upstream therefrom, at least one promoter and to a method of producing such human IL-2 protein which comprises cultivating said transformants to thereby cause production and accumulation of said protein in the culture and recovering the same.
摘要翻译： 本发明涉及用编码人IL-2蛋白的区域及其上游的至少一种启动子的DNA转化的动物细胞，以及生产这种人IL-2蛋白质的方法，包括培养所述转化体从而引起 所述蛋白质在培养物中的产生和积累并恢复。
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公开(公告)号：EP0582796A1
公开(公告)日：1994-02-16
申请号：EP93108651.6
申请日：1993-05-28
发明人： Shintani, Asae , Sasada, Reiko , Igarashi, Koichi
CPC分类号： C12N15/85 , A61K38/00 , C07K14/475 , C12N2830/008 , C12N2830/85
摘要： A novel DNA isolate encoding for a promoter region of human nerve growth factor-2, a recombinant vector containing said DNA, and a transformant carrying said recombinant vector are disclosed. The promoter of the present invention is useful as a means for analyzing an expression control mechanism of a nerve specific protein and can be used as a promoter which is incorporated into vectors used in the preparation of disease model animals, the treatment of diseases of organisms and the establishment of drug screening systems.
摘要翻译： 公开了编码人神经生长因子-2的启动子区域的新型DNA分离物，含有所述DNA的重组载体和携带所述重组载体的转化体。 本发明的启动子可用作分析神经特异性蛋白质的表达控制机制的手段，并且可用作引入到用于制备疾病模型动物的载体中的启动子，生物体疾病的治疗和 建立药物筛查系统。
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7.发明公开
公开(公告)号：EP0281822A2
公开(公告)日：1988-09-14
申请号：EP88102491.3
申请日：1988-02-20
CPC分类号： G01N33/6887 , A61K38/00 , C07K14/503 , C07K14/55 , C07K16/22 , C07K2319/00 , C07K2319/02 , C07K2319/75
摘要： A mutein of basic fibroblast growth factors (bFGF) possesses fibroblast growth promoting activity, growth stimulating activity of capillary endothelial cells and angiogenic activity, has high stability and is low toxicity. And therefore, the present mutein is advantageously used as a healing accelerator for burns etc., a therapeutic drug for thrombosis etc., and a cell cultivation promoter.
摘要翻译： 碱性成纤维细胞生长因子（bFGF）的突变蛋白具有成纤维细胞生长促进活性，毛细血管内皮细胞生长刺激活性和血管生成活性，具有高稳定性和低毒性。 因此，本发明的突变蛋白有利地用作燃烧等的愈合促进剂，血栓形成治疗药等，以及细胞培养促进剂。
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公开(公告)号：EP0237966A3
公开(公告)日：1988-03-16
申请号：EP87103601
申请日：1987-03-12
CPC分类号： C07K14/503
摘要： A human basic fibroblast growth factor (hbFGF), which is usable for a cure promoter for burns etc., therapeutic agent for thrombosis etc. or a reagent for promoting cell cultivation, can be produced in a large quantity by cultivating a transformant as transformed with a vector including a DNA coding the hbFGF.
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公开(公告)号：EP0386752B1
公开(公告)日：1994-09-14
申请号：EP90104419.8
申请日：1990-03-08
CPC分类号： C07K16/22 , A61K38/00 , C07K14/475 , C07K14/48
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公开(公告)号：EP0386752A1
公开(公告)日：1990-09-12
申请号：EP90104419.8
申请日：1990-03-08
CPC分类号： C07K16/22 , A61K38/00 , C07K14/475 , C07K14/48
摘要： Disclosed are (1) a polypeptide (I) including the following amino acid sequence (II) in a molecule thereof:
TyrAlaGluHisLysSerHisArgGlyGluTyrSerValCys
AspSerGluSerLeuTrpValThrAspLysSerSerAlaIle
AspIleArgGlyHisGlnValThrValLeuGlyGluIleLys
ThrGlyAsnSerProValLysGlnTyrPheTyrGluThrArg
CysLysGluAlaArgProValLysAsnGlyCysArgGlyIle
AspAspLysHisTrpAsnSerGlnCysLysThrSerGlnThr
TyrValArgAlaLeuThrSerGluAsnAsnLysLeuValGly
TrpArgTrpIleArgIleAspThrSerCysValCysAlaLeu
SerArgLysIleGlyArg (II)
(2) a DNA sequence coding for the polypeptide described in (1), (3) a vector including the DNA described in (2), (4) a transformant transformed by the vector described in (3), and (5) a process for producing the polypeptide (I) which comprises cultivating the transformant described in (4) in a culture medium to produce and accumulate the polypeptide described in (1) in a culture. The polypeptide is useful as a reagent for studies relating to the differentiation, growth and survival of animal cells, and may also be useful as a drug.摘要翻译： 本发明公开了（1）的多肽（I），包括在其分子中的下列氨基酸序列（II）：TyrAlaGluHisLysSerHisArgGlyGluTyrSerValCys AspSerGluSerLeuTrpValThrAspLysSerSerAlaIle AspIleArgGlyHisGlnValThrValLeuGlyGluIleLys ThrGlyAsnSerProValLysGlnTyrPheTyrGluThrArg CysLysGluAlaArgProValLysAsnGlyCysArgGlyIle AspAspLysHisTrpAsnSerGlnCysLysThrSerGlnThr TyrValArgAlaLeuThrSerGluAsnAsnLysLeuValGly TrpArgTrpIleArgIleAspThrSerCysValCysAlaLeu SerArgLysIleGlyArg（II）（2）的DNA序列编码中所述的多肽（1 ），（3）包含（2），（4）中描述的DNA的载体，由（3）中描述的载体转化的转化体，和（5）生产多肽（I）的方法，其包括培养所述转化体 在（4）中，在培养基中产生并积累（1）中描述的多肽。 该多肽可用作关于动物细胞的分化，生长和存活的研究的试剂，并且也可用作药物。
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