公开(公告)号：EP4407038A1

公开(公告)日：2024-07-31

申请号：EP22872052.0

申请日：2022-09-22

申请人： Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences

发明人： MA, Yanhe ,  CAI, Tao ,  SUN, Hongbing ,  WANG, Qinhong ,  WANG, Guokun ,  XU, Zhaoyu ,  ZHU, Zhiguang ,  MA, Chunling ,  ZHENG, Ping ,  WANG, Yu ,  QIAO, Jing ,  DONG, Hongjun ,  GUO, Wei ,  ZHOU, Hongyi

IPC分类号： C12P19/04 ,  C12P19/18 ,  C12N1/21 ,  C12N1/32

CPC分类号： Y02P20/133 ,  C12R2001/0120210501 ,  C12R2001/1520210501 ,  C12N1/38 ,  C12N15/80 ,  C12R2001/1920210501 ,  C12N9/10 ,  C12N15/81 ,  C12N15/70 ,  C12R2001/64520210501 ,  C12P19/04 ,  C12N1/32 ,  C12N15/74 ,  C12N15/77 ,  C12N15/113 ,  C12P19/18 ,  C12R2001/8420210501

摘要： Provided are a method for preparing starch using carbon dioxide, a recombinant microorganism, a method for constructing the recombinant microorganism, and a reagent. The method for preparing starch using carbon dioxide comprises: (1) providing energy and carbon sources for microbial cells on the basis of carbon dioxide and extracellular non-optical energy; and (2) generating starch within the microbial cells on the basis of at least one of up-regulated glucose-1-phosphate adenylyltransferase and starch synthase in the microbial cells. In this way, by utilizing non-optical energy, such as electric energy or hydrogen energy, starch can be effectively prepared inside the microbial cells by fixing carbon dioxide.

公开(公告)号：EP4202052A1

公开(公告)日：2023-06-28

申请号：EP21860265.4

申请日：2021-08-19

申请人： Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences

发明人： MA, Yanhe ,  CAI, Tao ,  SUN, Hongbing ,  QIAO, Jing ,  ZHANG, Fan ,  ZHANG, Jie ,  WANG, Qinhong

IPC分类号： C12P19/04 ,  C12P19/14 ,  C12N15/54

摘要： Provided is a starch biosynthesis method. The method may implement total artificial biosynthesis from simple compounds such as dihydroxyacetone, formaldehyde, formic acid and methanol to starch, and by coupling with methods such as chemical reduction of carbon dioxide, even total artificial biosynthesis of starch taking carbon dioxide as a starting raw material can be implemented. Natural starch synthesis is required to be subjected to the Calvin cycle, needing 21-22 reaction steps in total, and the present method merely needs 9-12 reaction steps. Nearly a half of the reaction steps is reduced, and a production cycle is greatly shortened. In addition, the present method can utilize carbon dioxide of high concentration and high density and electric energy and hydrogen energy of high energy density, is more suitable for an industrial production mode, and the production cycle is shortened from several months in farming to several days.