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    METHODS FOR ENHANCING INFECTIVITY OF RETROVIRUSES
    2.
    发明公开
    METHODS FOR ENHANCING INFECTIVITY OF RETROVIRUSES 有权
    法提高逆转录病毒的感染

    公开(公告)号：EP2432885A2

    公开(公告)日：2012-03-28

    申请号：EP10778039.7

    申请日：2010-05-18

    申请人： The Scripps Research Institute

    发明人： SAPHIRE, Andrew

    IPC分类号： C12N15/867 ,  C12N15/61 ,  C12N7/01

    CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N9/90 ,  C12N2740/10052 ,  C12N2740/13051 ,  C12N2740/13052 ,  C12N2740/15051 ,  C12N2740/15052 ,  C12N2740/16051 ,  C12N2740/16052

    摘要： The present invention provides methods for producing retroviruses or viral vectors with enhanced infectivity. The methods entail transfecting a retroviral vector into a packaging cell that has suppressed expression or inhibited enzymatic activity of a parvulin prolyl peptidyl isomerase (parvulin PPIase), and culturing the transfected packaging cell to allow production of viral particles. The invention also provides methods for enhancing efficiency of gene transfer with a recombinant retrovirus. These methods involve constructing a recombinant retroviral vector expressing a target gene, transfecting into a packaging cell that has suppressed expression or inhibited enzymatic activity of a parvulin prolyl peptidyl isomerase (parvulin PPIase), culturing the transfected packaging cell to allow production of recombinant retroviral particles, harvesting recombinant retroviral particles from supernatant of the cultured cell, and transducing the recombinant retroviral particles into a target cell. Kits for carrying out these methods are also provided in the invention.

    METHODS OF IMPROVING TITER IN TRANSFECTION-BASED PRODUCTION SYSTEMS USING EUKARYOTIC CELLS
    7.
    发明公开
    METHODS OF IMPROVING TITER IN TRANSFECTION-BASED PRODUCTION SYSTEMS USING EUKARYOTIC CELLS 审中-公开
    VERFAHREN ZUR TITER-VERBESSERUNG在TRANSFEKTIONSBASIERTEN PRODUKTIONSSYSTEMEN MITTELS EUKARYOTISCHER ZELLEN

    公开(公告)号：EP3022306A4

    公开(公告)日：2017-01-11

    申请号：EP14826342

    申请日：2014-07-18

    申请人： CINCINNATI CHILDREN'S HOSPITAL MEDICAL CENTER

    发明人： VAN DER LOO JOHANNES ,  MALIK PUNAM

    IPC分类号： C12N15/86

    CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N2740/10043 ,  C12N2740/10051 ,  C12N2740/13051 ,  C12N2740/16051

    摘要： The invention relates to methods of improving titer in transfection-based bioreactor culture production or transfection-based production systems using eukaryotic cells. A method of improving viral titer in a transfectionbased production system using a eukaryotic cell is provided. The method can include at least one of: harvesting a confluent population of eukaryotic cells that have progressed beyond log phase of cell growth for at least 24 hours prior to transfection; transfecting the cells by mixing the population with transfection reagents and plasmid DNA at the time of re-seeding the cells into a culture vessel, where the harvesting and transfecting steps, alone or in combination, results in an improved viral titer, by at least 2-fold, in a transfectionbased production using a eukaryotic cell.

    摘要翻译： 本发明涉及在基于转染的生物反应器培养生产或使用真核细胞的基于转染的生产系统中提高滴度的方法。 提供了一种在使用真核细胞的基于转染的生产系统中改善病毒滴度的方法。 该方法可以包括以下至少之一：在转染前至少24小时收获已经进展超过细胞生长对数期的真核细胞汇合群体; 在将细胞再次接种到培养容器中时，将群体与转染试剂和质粒DNA混合，其中单独或组合的收获和转染步骤导致改善的病毒滴度至少2 在使用真核细胞的基于转染的生产中。