公开(公告)号：EP0564958A1

公开(公告)日：1993-10-13

申请号：EP93105226.0

申请日：1993-03-30

申请人： BOEHRINGER MANNHEIM GMBH

发明人： Vonwirth, Heiner, Dr. ,  Marschall, Michael, Dr. ,  Bayer, Hubert, Dr.

IPC分类号： C12N7/06 ,  G01N33/53 ,  G01N33/569

CPC分类号： C12N7/00 ,  C12N2770/32451 ,  G01N33/56983 ,  Y02A50/54 ,  Y10S435/962 ,  Y10S436/82 ,  Y10S436/826

摘要： Die Erfindung betrifft eine Viruslösung, die ein Detergens der allgemeinen Formel I enthält und der Stabilisierung der Viruslösung dient sowie deren Herstellung und Verwendung in heterogenen Immunoassays zum Nachweis von Antikörpern gegen Viren.

摘要翻译： 本发明涉及一种病毒溶液，其含有用于稳定病毒溶液的通式I的洗涤剂，以及用于检测针对病毒的抗体的非均相免疫测定中的制备及其用途。

公开(公告)号：EP0468702A3

公开(公告)日：1992-02-05

申请号：EP91306541.3

申请日：1991-07-18

申请人： MERCK & CO. INC.

发明人： Lewis, John A. ,  Armstrong, Marcy Ellen

IPC分类号： C12N7/02 ,  A61K39/29

CPC分类号： A61K39/125 ,  A61K39/12 ,  C12N2770/32434 ,  C12N2770/32451

摘要： New methods for purifying Hepatitis-A virus (HAV) capsids are amenable to commercial scale-up and manufacture of specific HAV vaccines, including formalin-inactivated HAV and attenuated HAV.

公开(公告)号：EP0413637A1

公开(公告)日：1991-02-20

申请号：EP90402299.3

申请日：1990-08-16

申请人： ZHEJIANG ACADEMY OF MEDICAL SCIENCES

发明人： Mao,Jiang-sen ,  Dong,De-xiang ,  Chen,Nian-liang ,  Zhang,Hua-yuan ,  Huang,Hai-ying ,  Zhang,Shu-ya ,  Cao,Yi-yun ,  Cai,Shao-ai ,  Xie,Ru-ying ,  Lian,You-hui ,  D.Q. Chen ,  Zhang,Hang-chun ,  Li,Han-min ,  Zhu,Ji-ming

IPC分类号： A61K39/29 ,  C12N7/08

CPC分类号： A61K39/29 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/54 ,  A61K2039/542 ,  A61K2039/57 ,  C12N2770/32434 ,  C12N2770/32451 ,  C12N2770/32461 ,  Y02A50/464

摘要： The strain is obtained by means of isolation of virus from cells of tissue culture. It is characterized by serial passages through tissue cells, culture at low temperature and is adapted to grow in human lung diploid fibroblasts, so as to develop the vaccine (H₂strain) for prophylaxis of human hepatitis A.
The monkey model is also established for experimental trials.

摘要翻译： 通过从组织培养的细胞中分离病毒获得菌株。 其特征在于通过组织细胞的连续传代，低温培养并适于在人肺二倍体成纤维细胞中生长，以开发用于预防人类甲型肝炎的疫苗（H2strain）。猴模型也被建立用于实验试验 。

公开(公告)号：EP1599580A1

公开(公告)日：2005-11-30

申请号：EP04715891.0

申请日：2004-03-01

申请人： Glaxosmithkline Biologicals S.A.

发明人： AERTS, Brigitte, Ghislaine, Louise ,  GHISLAIN, Yves, Jules, Maurice ,  GONZE, Marie-Monique, Jane ,  KNOTT, Isabelle, Solange, Lucie ,  MAGGETTO, CarineGlaxoSmithKline Biologicals S.A.

IPC分类号： C12N5/06 ,  C12N7/02

CPC分类号： C12N7/00 ,  C12N5/0018 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/39 ,  C12N2770/32451

摘要： The present invention relates to a process for animal, preferably human, diploid anchorage-dependent cell culture, in the absence of exogenous components of primary animal origin, and to a cell culture medium substantially free of exogenous components of primary animal origin suitable for carrying out said process. In particular the invention concerns a cell culture medium which comprises at least one, more preferably several, exogenous animal-free growth factors. The present invention also relates to a process for cultivating animal, preferably human diploid anchorage-dependent cells in a medium according to the invention, involving the use of a trypsin substitute of non-animal origin for passaging cells. The invention further relates to a process for producing viruses, viral vaccines and the like.

公开(公告)号：EP1000174A4

公开(公告)日：2004-08-04

申请号：EP98930378

申请日：1998-06-18

申请人： NEW YORK BLOOD CT INC

发明人： LEE DONG-HUN ,  PRINCE ALFRED M

IPC分类号： C12P19/34 ,  C12Q20060101 ,  C12Q1/68 ,  C12Q1/70

CPC分类号： C12Q1/6806 ,  B01D61/14 ,  B01D71/32 ,  C12N2730/10151 ,  C12N2740/16051 ,  C12N2770/24251 ,  C12N2770/32451 ,  C12Q1/701 ,  C12Q2527/156 ,  C12Q2563/155

公开(公告)号：EP0790836A4

公开(公告)日：2003-05-28

申请号：EP95941434

申请日：1995-11-30

申请人： DYNCORP ,  NAT INST HEALTH

发明人： CHANOCK ROBERT M ,  PURCELL ROBERT H ,  KAPIKIAN ALBERT Z ,  POTASH LOUIS

IPC分类号： C12N5/00 ,  A61K39/12 ,  A61K39/125 ,  A61K39/13 ,  A61K39/15 ,  A61K39/29 ,  A61P31/12 ,  C12N7/00 ,  C12N7/04 ,  A61K39/145 ,  C12N5/06 ,  C12N7/02

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K2039/5252 ,  A61K2039/5254 ,  C12N2720/12334 ,  C12N2720/12351 ,  C12N2740/10051 ,  C12N2740/14051 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16234 ,  C12N2760/16251 ,  C12N2760/18534 ,  C12N2760/18551 ,  C12N2760/18634 ,  C12N2760/18651 ,  C12N2760/18734 ,  C12N2760/18751 ,  C12N2770/12034 ,  C12N2770/12051 ,  C12N2770/32334 ,  C12N2770/32351 ,  C12N2770/32434 ,  C12N2770/32451 ,  C12N2770/32634 ,  C12N2770/32651

摘要： A novel African Green Monkey Kidney (AGMK) cell line is taught as well as a method for the preparation thereof. The cell line which is free of viable adventitious microbial agents is useful as a substrate for viruses and for the preparation of viral vaccines.

公开(公告)号：EP0790836A1

公开(公告)日：1997-08-27

申请号：EP95941434.0

申请日：1995-11-30

申请人： DYNCORP ,  NATIONAL INSTITUTES OF HEALTH

发明人： CHANOCK, Robert M. ,  PURCELL, Robert H. ,  KAPIKIAN, Albert Z. ,  POTASH, Louis

IPC分类号： C12N5 ,  A61K39 ,  A61P31 ,  C12N7

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K2039/5252 ,  A61K2039/5254 ,  C12N2720/12334 ,  C12N2720/12351 ,  C12N2740/10051 ,  C12N2740/14051 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16234 ,  C12N2760/16251 ,  C12N2760/18534 ,  C12N2760/18551 ,  C12N2760/18634 ,  C12N2760/18651 ,  C12N2760/18734 ,  C12N2760/18751 ,  C12N2770/12034 ,  C12N2770/12051 ,  C12N2770/32334 ,  C12N2770/32351 ,  C12N2770/32434 ,  C12N2770/32451 ,  C12N2770/32634 ,  C12N2770/32651 ,  Y02A50/466

摘要： A novel African Green Monkey Kidney (AGMK) cell line is taught as well as a method for the preparation thereof. The cell line which is free of viable adventitious microbial agents is useful as a substrate for viruses and for the preparation of viral vaccines.

公开(公告)号：EP0468702A2

公开(公告)日：1992-01-29

申请号：EP91306541.3

申请日：1991-07-18

申请人： MERCK & CO. INC.

发明人： Lewis, John A. ,  Armstrong, Marcy Ellen

IPC分类号： C12N7/02 ,  A61K39/29

CPC分类号： A61K39/125 ,  A61K39/12 ,  C12N2770/32434 ,  C12N2770/32451

摘要： New methods for purifying Hepatitis-A virus (HAV) capsids are amenable to commercial scale-up and manufacture of specific HAV vaccines, including formalin-inactivated HAV and attenuated HAV.

摘要翻译： 用于纯化甲型肝炎病毒（HAV）衣壳的新方法适用于商业化扩增和制造特定HAV疫苗，包括福尔马林灭活的HAV和减毒的HAV。

公开(公告)号：EP0302692A2

公开(公告)日：1989-02-08

申请号：EP88307100.3

申请日：1988-08-01

申请人： Merck & Co., Inc.

发明人： Lewis, John A. ,  Emini, Emilio A. ,  Armstrong, Marcy E.

IPC分类号： C12N7/02

CPC分类号： A61K39/29 ,  A61K39/12 ,  A61K2039/5254 ,  C12N2770/32434 ,  C12N2770/32451 ,  Y02A50/464

摘要： New methods for purifying Hepatitis A virus (HAV) are amendable to commercial scale-up and manufacture of specific HAV vaccines, including formalin-inactivated HAV and attenuated HAV.

摘要翻译： 用于纯化甲型肝炎病毒（HAV）的新方法可以修改为商业扩大和制造特定HAV疫苗，包括福尔马林灭活的HAV和减毒的HAV。

公开(公告)号：EP1453538A2

公开(公告)日：2004-09-08

申请号：EP02792955.3

申请日：2002-12-10

申请人： Baxter Healthcare S.A.

发明人： TAUER, Christa ,  MEYER, Heidi ,  MITTERER, Artur ,  BARRETT, Noel

IPC分类号： A61K39/29 ,  C12N7/02

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/29 ,  A61K2039/5252 ,  A61K2039/55505 ,  A61K2039/57 ,  C12N2770/32432 ,  C12N2770/32451 ,  Y02A50/464 ,  Y10S435/80 ,  Y10S435/803 ,  Y10S435/814 ,  Y10S435/816 ,  Y10S435/962

摘要： The present invention provides methods of purification of Hepatitis A Virus from the supernatant of an infected cell culture and production of a preparation of purified HAV antigen. The present invention is also directed to an HAV vaccine composition comprising a preparation consisting of purified mature HAV particles in an amount sufficient to induce a protective immune response in a mammal.