公开(公告)号：EP3070176A4

公开(公告)日：2017-05-03

申请号：EP14863005

申请日：2014-11-13

申请人： TOKYO METROPOLITAN INST MEDICAL SCIENCE

发明人： YOSHIDA YUKIKO ,  SAEKI YASUSHI ,  TSUCHIYA HIKARU ,  MURAKAMI ARISA ,  TANAKA KEIJI

IPC分类号： C12Q1/527 ,  C12N15/09 ,  C12Q1/37

CPC分类号： G01N33/6842 ,  C12Q1/25 ,  C12Q1/527 ,  G01N2440/36

摘要： An object of the present invention is to provide a method for efficiently identifying a polyubiquitinated substrate which is generally not easily identified. The method for identifying a polyubiquitinated substrate includes (1) a step of expressing a trypsin-resistant polyubiquitin chain-binding protein and a ubiquitin ligase in a cell, (2) a step of isolating a complex that contains the trypsin-resistant polyubiquitin chain-binding protein from the cell having undergone the step (1), (3) a step of subjecting the complex isolated by the step (2) to trypsin digestion, and (4) a step of identifying a peptide that has a ubiquitination site from a digested material obtained by the step (3).

摘要翻译： 本发明的一个目的是提供一种用于有效鉴定通常不易鉴定的多聚遍在蛋白化底物的方法。 鉴定多聚泛素化底物的方法包括：（1）在细胞中表达胰蛋白酶抗性多聚遍在蛋白链结合蛋白和泛蛋白连接酶的步骤;（2）分离含有胰蛋白酶抗性多聚遍在蛋白链结合蛋白的复合物的步骤; （3）使步骤（2）分离的复合物进行胰蛋白酶消化的步骤，和（4）从步骤（1）获得的具有泛素化位点的肽的步骤。 （3）获得的消化材料。

公开(公告)号：EP2558496A1

公开(公告)日：2013-02-20

申请号：EP11769579.1

申请日：2011-04-14

申请人： Genentech, Inc.

发明人： DIXIT, Vishva ,  KELLEY, Robert F. ,  MATSUMOTO, Marissa L.

IPC分类号： C07K16/18 ,  A61K39/395

CPC分类号： C07K16/18 ,  C07K14/4702 ,  C07K2317/24 ,  C07K2317/30 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/92 ,  C12N15/1037 ,  G01N33/68 ,  G01N2333/4703 ,  G01N2440/36

摘要： The invention provides anti-polyubiquitin antibodies and methods of using the same.

公开(公告)号：EP2363496A1

公开(公告)日：2011-09-07

申请号：EP10015840.1

申请日：2005-08-18

申请人： Odyssey Thera, Inc.

发明人： Westwick, John ,  Yu, Helen ,  MacDonald, Marnie L.

IPC分类号： C12Q1/02 ,  G01N33/50

CPC分类号： C12Q1/025 ,  G01N33/5023 ,  G01N33/5035 ,  G01N33/5041 ,  G01N33/5097 ,  G01N2440/00 ,  G01N2440/10 ,  G01N2440/12 ,  G01N2440/36 ,  G01N2440/38 ,  G01N2500/10

摘要： The instant invention provides a method for establishing safety profiles for chemical compounds, as well as pharmacological profiling said method comprising (A) testing the effects of said chemical compounds on the amount and/or post-translational modifications of two or more macromolecules in intact cells; (B) constructing a pharmacological profile based on the results of said tests; and (C) comparing said profile to the profile(s) of drugs with established safety characteristics. Additionally, the invention is also directed to a composition comprising an assay panel, said panel comprising at least one high-content assay for the amount and/or post-translational modification of a protein and at least one high-content assay for the amount and/or subcellular location of a protein-protein interaction.

摘要翻译： 本发明提供了建立化学化合物安全性概况的方法以及药理概况分析，所述方法包括（A）测试所述化学化合物对完整细胞中两种或更多种大分子的量和/或翻译后修饰的影响 ; （B）基于所述测试的结果构建药理学特征; 和（C）将所述特征与具有确定安全特征的药物特征进行比较。 此外，本发明还涉及包含测定组的组合物，所述组包含至少一种蛋白质的量和/或翻译后修饰的高含量测定和至少一种高含量测定的量以及 /或蛋白质 - 蛋白质相互作用的亚细胞定位。

公开(公告)号：EP2247617A2

公开(公告)日：2010-11-10

申请号：EP09731152.6

申请日：2009-01-16

申请人： Genentech, Inc.

发明人： KELLEY, Robert, F. ,  MATSUMOTO, Marissa, L.

IPC分类号： C07K16/18 ,  G01N33/68

CPC分类号： C07K16/18 ,  C07K14/47 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/35 ,  C07K2317/51 ,  C07K2317/515 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/92 ,  G01N33/68 ,  G01N2333/47 ,  G01N2440/36

摘要： Anti-K63-linked polyubiquitin monoclonal antibodies, and methods for using the antibodies, are provided.

公开(公告)号：EP2954905A4

公开(公告)日：2016-11-09

申请号：EP13868860

申请日：2013-12-26

申请人： NAKAJIMA TOSHIHIRO

发明人： NAKAJIMA TOSHIHIRO ,  FUJITA HIDETOSHI ,  ARATANI SATOKO ,  YAGISHITA NAOKO

IPC分类号： A61K45/00 ,  A61P3/04 ,  A61P43/00 ,  C12Q1/68 ,  G01N33/15 ,  G01N33/50

CPC分类号： G01N33/573 ,  C12Q1/6883 ,  C12Q2600/136 ,  C12Q2600/158 ,  G01N33/5044 ,  G01N33/6875 ,  G01N2333/70567 ,  G01N2333/9015 ,  G01N2440/36 ,  G01N2500/04 ,  G01N2500/10

摘要： [Problem] To provide a mitochondria-function regulator effective for treatment or prevention of obesity, and a screening method therefore. [Solution] The mitochondria-function regulator of the present invention contains, as an active ingredient, a PGC-1² - protein function regulator synoviolin. The screening method of the present invention includes a step for causing a test substance to act on an adipose tissue cell or an individual animal, and measuring or detecting one or more of the following in the adipose tissue cell: (1) expression level of synoviolin; (2) a bond between synoviolin and PGC-1² protein; and (3) the ubiquitination of the PGC-1² protein by synoviolin.

摘要翻译： [问题]为了提供一种线粒体功能调节剂有效地用于治疗或预防肥胖症，因此筛选方法。 [解决方案本发明的线粒体功能调节剂含有作为活性成分的，一个PGC-1² - 蛋白功能调节synoviolin。 本发明的筛选方法包括用于使被检物质在脂肪组织细胞或个体动物作用于，并测量或检测在脂肪组织细胞以下的一个或多个步骤：（1）synoviolin的表达水平 ; （2）synoviolin和PGC-1²蛋白之间的键; 和（3）蛋白PGC-1²通过synoviolin的泛素化。

公开(公告)号：EP2772548A1

公开(公告)日：2014-09-03

申请号：EP13156942.8

申请日：2013-02-27

申请人： Universität Ulm

发明人： Johnsson, Nils ,  Moreno Andrés, Daniel ,  Dünkler, Alexander

IPC分类号： C12Q1/02 ,  C12Q1/37 ,  G01N33/50 ,  G01N33/542 ,  G01N33/68

CPC分类号： C12Q1/025 ,  C12Q1/37 ,  G01N33/5008 ,  G01N33/542 ,  G01N33/6845 ,  G01N2440/36

摘要： The invention relates to a fluorescent reporter for determining molecular interactions, comprising an ubiquitin sub-domain attached to two flanking fluorescent marker molecules, and a system and method for determining molecular interactions, preferably between a protein and another molecule.

摘要翻译： 本发明涉及一种用于确定分子相互作用的荧光报告物，其包含连接到两个侧翼荧光标记分子的泛素亚结构域，以及用于确定分子相互作用，优选在蛋白质和另一分子之间的系统和方法。

公开(公告)号：EP2638394A4

公开(公告)日：2014-06-18

申请号：EP11839316

申请日：2011-11-14

申请人： NORTHERN SYDNEY LOCAL HEALTH DISTR

发明人： MARSH DEBORAH JOY ,  HAHN MICHAEL ANTHONY

IPC分类号： G01N33/53 ,  C07K16/18 ,  C07K16/30 ,  G01N33/574

CPC分类号： G01N33/6893 ,  G01N33/57484 ,  G01N2440/36 ,  G01N2500/02

摘要： The present disclosure relates generally to the field of cancer diagnosis. More specifically, the present disclosure relates to the identification and use of monoubiquitination of histone 2B as a biomarker for the diagnosis and prognosis of cancer including, but not limited to, parathyroid cancer. The present disclosure also relates to the identification of binding between CDC73 and RNF20, and the use of CDC73 and RNF20 in an assay for screening for an agent that modulates monoubiquitination of a histone protein.

摘要翻译： 本公开一般涉及癌症诊断领域。 更具体地，本公开涉及组蛋白2B的单泛素化作为用于诊断和预后的癌症的生物标志物的鉴定和用途，包括但不限于甲状旁腺癌。 本公开还涉及鉴定CDC73和RNF20之间的结合，以及CDC73和RNF20在用于筛选调节组蛋白的单泛素化的试剂的测定中的用途。

公开(公告)号：EP2446883A1

公开(公告)日：2012-05-02

申请号：EP10189534.0

申请日：2010-10-30

申请人： University College Cork-National University of Ireland, Cork

发明人： Carmody, Ruadhri, Dr.

IPC分类号： A61K31/00 ,  A61K31/4015 ,  G01N33/50 ,  A61P29/00 ,  A61P9/00 ,  A61P25/28 ,  A61P35/00 ,  A61P37/06 ,  A61P9/10

CPC分类号： A61K31/00 ,  A61K31/4015 ,  C12N15/1137 ,  C12N2310/14 ,  C12Y301/02015 ,  G01N2333/916 ,  G01N2440/36 ,  G01N2500/02 ,  G01N2800/7095 ,  Y02A50/414

摘要： A method for the treatment of inflammation in an individual is described, for example a causal therapy of a disease or condition for which an inflammatory state is an underlying cause of the disease, or a treatment of inflammation as a symptom of a disease state, for example inflammation caused by cancer, cardiovascular disease, an infection or trauma, by treating the individual with an agent capable of attenuating the activity of Ubiquitin Serine Protease 7 (USP7) protein, in particular a USP7 inhibitor chemical.

摘要翻译： 描述了治疗个体炎症的方法，例如，疾病或病症的因果治疗，其中炎症状态是疾病的根本原因，或作为疾病状态的症状的炎症治疗，用于 例如通过用能够减弱泛素丝氨酸蛋白酶7（USP7）蛋白质，特别是USP7抑制剂化学品的活性的试剂治疗个体，由癌症，心血管疾病，感染或创伤引起的炎症。

公开(公告)号：EP1784642A2

公开(公告)日：2007-05-16

申请号：EP05788134.4

申请日：2005-08-18

申请人： Odyssey Thera, Inc.

发明人： WESTWICK, John, K. ,  YU, Helen ,  MACDONALD, Marnie, L.

IPC分类号： G01N33/53

CPC分类号： C12Q1/025 ,  G01N33/5023 ,  G01N33/5035 ,  G01N33/5041 ,  G01N33/5097 ,  G01N2440/00 ,  G01N2440/10 ,  G01N2440/12 ,  G01N2440/36 ,  G01N2440/38 ,  G01N2500/10

摘要： The instant invention provides a method for establishing safety profiles for chemical compounds, as well as pharmacological profiling said method comprising (A) testing the effects of said chemical compounds on the amount and/or post-translational modifications of two or more macromolecules in intact cells; (B) constructing a pharmacological profile based on the results of said tests; and (C) comparing said profile to the profile(s) of drugs with established safety characteristics. Additionally, the invention is also directed to a composition comprising an assay panel, said panel comprising at least one high-content assay for the amount and/or post-­translational modification of a protein and at least one high-content assay for the amount and/or subcellular location of a protein-protein interaction.

公开(公告)号：EP3362086A1

公开(公告)日：2018-08-22

申请号：EP16856197.5

申请日：2016-10-13

申请人： The Scripps Research Institute

发明人： YANG, Xiang-Lei ,  MO, Zhongying ,  SCHIMMEL, Paul

IPC分类号： A61K38/53 ,  A61P35/00 ,  C07H21/02 ,  C07K16/40 ,  C12N5/04 ,  C12N5/07 ,  C12Q1/48

CPC分类号： C12Q1/25 ,  G01N2440/36

摘要： Disclosed herein are methods and compositions for inhibiting neddylation using Glycyl-tRNA synthase (GlyRS) inhibitors. Also disclosed are related compositions and methods for treating diseases such as cancer.