公开(公告)号：EP2966985B1

公开(公告)日：2018-09-19

申请号：EP14770418.3

申请日：2014-03-10

申请人： Mayo Foundation for Medical Education and Research

发明人： BARNIDGE, David R. ,  MURRAY, David L.

IPC分类号： A01H1/06

CPC分类号： G01N33/6848 ,  G01N30/7233 ,  G01N33/57426 ,  G01N33/6851 ,  G01N33/6857 ,  G01N2800/52 ,  H01J49/0027 ,  H01J49/164

摘要： Disclosure herein are methods for determining whether or not an immunoglobulin is present above the polyclonal background level in a biological sample, and methods for determining whether an immunoglobulin contains a kappa or lambda light chain. These methods are useful for screening biological samples for the presence or absence of monoclonal gammopathy, and for diagnosing and monitoring monoclonal gammopathy in a subject.

公开(公告)号：EP3138623B1

公开(公告)日：2018-08-22

申请号：EP16181300.1

申请日：2009-01-14

申请人： Agena Bioscience, Inc.

发明人： BECKER, Thomas

IPC分类号： G01N33/68

CPC分类号： C12Q1/6872 ,  G01N33/6848 ,  G01N33/6851 ,  Y10T436/143333 ,  Y10T436/24 ,  Y10T436/25125

摘要： The invention provides a novel additive for improved analysis by mass spectrometry. More specifically, ascorbic acid has been found to reduce or eliminate the presence of adducts commonly present in mass spectra. The improved processes and compositions of the invention allow for increased accuracy, sensitivity and throughput for samples analyzed by mass spectrometry.

公开(公告)号：EP3214436A4

公开(公告)日：2018-07-04

申请号：EP16841741

申请日：2016-08-26

申请人： HAMAMATSU PHOTONICS KK

发明人： NAITO YASUHIDE ,  KOTANI MASAHIRO ,  OHMURA TAKAYUKI

IPC分类号： H01J49/04 ,  G01N27/62

CPC分类号： H01J49/0418 ,  G01N1/2806 ,  G01N1/2813 ,  G01N27/62 ,  G01N33/6851 ,  H01J49/164 ,  H01J49/26

摘要： A surface-assisted laser desorption/ionization method according to an aspect includes: a first process of preparing a sample support (2) having a substrate (21) in which a plurality of through-holes (S) passing from one surface (21a) thereof to the other surface (21b) thereof are provided and a conductive layer (23) that covers at least the one surface (21a); a second process of placing a sample (10) on a sample stage (1) and arranging the sample support (2) on the sample (10) such that the other surface (21b) faces the sample (10); and a third process of applying a laser beam (L) to the one surface (21a) and ionizing the sample (10) moved from the other surface (21b) side to the one surface (21 a) side via the through-holes (S) due to a capillary phenomenon.

公开(公告)号：EP3266037A1

公开(公告)日：2018-01-10

申请号：EP16709546.2

申请日：2016-03-07

申请人： Micromass UK Limited

发明人： JONES, Emrys ,  KARANCSI, Tamás ,  PRINGLE, Steven Derek ,  BALOG, Júlia ,  SIMON, Dániel ,  GÖDÖRHÁZY, Lajos ,  SZALAY, Dániel ,  TAKÁTS, Zoltán

IPC分类号： H01J49/04 ,  H01J49/06 ,  H01J49/16 ,  H01J49/00 ,  G01N27/62 ,  G01N33/68 ,  G01N33/92 ,  A61B10/02 ,  A61B18/14

CPC分类号： H01J49/049 ,  A61B1/00013 ,  A61B1/041 ,  A61B1/2736 ,  A61B1/31 ,  A61B5/0066 ,  A61B5/0075 ,  A61B5/015 ,  A61B5/0507 ,  A61B5/055 ,  A61B5/14542 ,  A61B6/032 ,  A61B6/037 ,  A61B8/13 ,  A61B10/00 ,  A61B10/0041 ,  A61B10/0233 ,  A61B10/0283 ,  A61B17/00 ,  A61B17/320068 ,  A61B18/00 ,  A61B18/04 ,  A61B18/042 ,  A61B18/14 ,  A61B18/1445 ,  A61B18/1815 ,  A61B18/20 ,  A61B90/13 ,  A61B2010/0083 ,  A61B2017/320069 ,  A61B2018/00577 ,  A61B2018/00589 ,  A61B2018/00994 ,  A61B2218/002 ,  A61B2218/008 ,  A61F13/38 ,  C12Q1/025 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/24 ,  G01N1/2202 ,  G01N3/00 ,  G01N9/00 ,  G01N27/622 ,  G01N27/624 ,  G01N30/724 ,  G01N33/487 ,  G01N33/48735 ,  G01N33/6848 ,  G01N33/6851 ,  G01N33/92 ,  G01N2001/2223 ,  G01N2333/195 ,  G01N2405/00 ,  G01N2405/04 ,  G01N2405/08 ,  G01N2570/00 ,  G01N2800/26 ,  G06F19/18 ,  G06F19/324 ,  G06F19/3481 ,  G16H10/40 ,  G16H15/00 ,  G16H50/20 ,  H01J49/0004 ,  H01J49/0027 ,  H01J49/0031 ,  H01J49/0036 ,  H01J49/025 ,  H01J49/0404 ,  H01J49/0409 ,  H01J49/0422 ,  H01J49/044 ,  H01J49/0445 ,  H01J49/0459 ,  H01J49/0463 ,  H01J49/0468 ,  H01J49/061 ,  H01J49/068 ,  H01J49/10 ,  H01J49/14 ,  H01J49/16 ,  H01J49/164 ,  H01J49/24 ,  H01J49/26

摘要： A method of mass spectrometry or ion mobility spectrometry is disclosed comprising: providing an analyte; supplying a matrix compound to said analyte such that said analyte dissolves in said matrix; forming first droplets of the dissolved analyte; and colliding said first droplets with a collision surface. The use of matrix improves the analyte ion signal.

公开(公告)号：EP3266035A1

公开(公告)日：2018-01-10

申请号：EP16710788.7

申请日：2016-03-07

申请人： Micromass UK Limited

发明人： KARANCSI, Tamás ,  SIMON, Dániel ,  GÖDÖRHÁZY, Lajos ,  SZALAY, Dániel ,  PRINGLE, Steven Derek ,  JONES, Emrys ,  TRIVETT, Ian ,  O'BRIEN, Stephen ,  HESSE, Anthony ,  HENDERSON, Matt ,  CHUA, Alvin ,  TAKÁTS, Zoltán

IPC分类号： H01J49/00 ,  H01J49/04 ,  H01J49/06 ,  H01J49/16 ,  G01N33/68 ,  G01N33/92 ,  G01N27/62 ,  A61B10/02 ,  A61B18/14

CPC分类号： H01J49/049 ,  A61B1/00013 ,  A61B1/041 ,  A61B1/2736 ,  A61B1/31 ,  A61B5/0066 ,  A61B5/0075 ,  A61B5/015 ,  A61B5/0507 ,  A61B5/055 ,  A61B5/14542 ,  A61B6/032 ,  A61B6/037 ,  A61B8/13 ,  A61B10/00 ,  A61B10/0041 ,  A61B10/0233 ,  A61B10/0283 ,  A61B17/00 ,  A61B17/320068 ,  A61B18/00 ,  A61B18/04 ,  A61B18/042 ,  A61B18/14 ,  A61B18/1445 ,  A61B18/1815 ,  A61B18/20 ,  A61B90/13 ,  A61B2010/0083 ,  A61B2017/320069 ,  A61B2018/00577 ,  A61B2018/00589 ,  A61B2018/00994 ,  A61B2218/002 ,  A61B2218/008 ,  A61F13/38 ,  C12Q1/025 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/24 ,  G01N1/2202 ,  G01N3/00 ,  G01N9/00 ,  G01N27/622 ,  G01N27/624 ,  G01N30/724 ,  G01N33/487 ,  G01N33/48735 ,  G01N33/6848 ,  G01N33/6851 ,  G01N33/92 ,  G01N2001/2223 ,  G01N2333/195 ,  G01N2405/00 ,  G01N2405/04 ,  G01N2405/08 ,  G01N2570/00 ,  G01N2800/26 ,  G06F19/18 ,  G06F19/324 ,  G06F19/3481 ,  G16H10/40 ,  G16H15/00 ,  G16H50/20 ,  H01J49/0004 ,  H01J49/0027 ,  H01J49/0031 ,  H01J49/0036 ,  H01J49/025 ,  H01J49/0404 ,  H01J49/0409 ,  H01J49/0422 ,  H01J49/044 ,  H01J49/0445 ,  H01J49/0459 ,  H01J49/0463 ,  H01J49/0468 ,  H01J49/061 ,  H01J49/068 ,  H01J49/10 ,  H01J49/14 ,  H01J49/16 ,  H01J49/164 ,  H01J49/24 ,  H01J49/26

摘要： A method is disclosed comprising providing a biological sample on a swab, directing a spray of charged droplets onto a surface of the swab in order to generate a plurality of analyte ions, and analysing the analyte ions.

公开(公告)号：EP3265820A1

公开(公告)日：2018-01-10

申请号：EP16710787.9

申请日：2016-03-07

申请人： Micromass UK Limited

发明人： TAKÁTS, Zoltán ,  BOLT, Frances ,  KARANCSI, Tamás ,  JONES, Emrys ,  RICHARDSON, Keith ,  GÖDÖRHÁZY, Lajos ,  SZALAY, Dániel ,  BALOG, Júlia ,  PRINGLE, Steven Derek ,  SIMON, Dániel

IPC分类号： G01N33/68 ,  G01N33/02 ,  G01N33/48 ,  G01N27/62 ,  A61B17/00 ,  A61B18/00 ,  G01N3/00 ,  G01N9/00 ,  H01J49/00

CPC分类号： H01J49/049 ,  A61B1/00013 ,  A61B1/041 ,  A61B1/2736 ,  A61B1/31 ,  A61B5/0066 ,  A61B5/0075 ,  A61B5/015 ,  A61B5/0507 ,  A61B5/055 ,  A61B5/14542 ,  A61B6/032 ,  A61B6/037 ,  A61B8/13 ,  A61B10/00 ,  A61B10/0041 ,  A61B10/0233 ,  A61B10/0283 ,  A61B17/00 ,  A61B17/320068 ,  A61B18/00 ,  A61B18/04 ,  A61B18/042 ,  A61B18/14 ,  A61B18/1445 ,  A61B18/1815 ,  A61B18/20 ,  A61B90/13 ,  A61B2010/0083 ,  A61B2017/320069 ,  A61B2018/00577 ,  A61B2018/00589 ,  A61B2018/00994 ,  A61B2218/002 ,  A61B2218/008 ,  A61F13/38 ,  C12Q1/025 ,  C12Q1/04 ,  C12Q1/18 ,  C12Q1/24 ,  G01N1/2202 ,  G01N3/00 ,  G01N9/00 ,  G01N27/622 ,  G01N27/624 ,  G01N30/724 ,  G01N33/487 ,  G01N33/48735 ,  G01N33/6848 ,  G01N33/6851 ,  G01N33/92 ,  G01N2001/2223 ,  G01N2333/195 ,  G01N2405/00 ,  G01N2405/04 ,  G01N2405/08 ,  G01N2570/00 ,  G01N2800/26 ,  G06F19/18 ,  G06F19/324 ,  G06F19/3481 ,  G16H10/40 ,  G16H15/00 ,  G16H50/20 ,  H01J49/0004 ,  H01J49/0027 ,  H01J49/0031 ,  H01J49/0036 ,  H01J49/025 ,  H01J49/0404 ,  H01J49/0409 ,  H01J49/0422 ,  H01J49/044 ,  H01J49/0445 ,  H01J49/0459 ,  H01J49/0463 ,  H01J49/0468 ,  H01J49/061 ,  H01J49/068 ,  H01J49/10 ,  H01J49/14 ,  H01J49/16 ,  H01J49/164 ,  H01J49/24 ,  H01J49/26

摘要： A method of analysis using mass spectrometry and/or ion mobility spectrometry is disclosed. The method comprises: using a first device to generate smoke, aerosol or vapour from a target comprising or consisting of a microbial population; mass analysing and/or ion mobility analysing said smoke, aerosol or vapour, or ions derived therefrom, in order to obtain spectrometric data; and analysing said spectrometric data in order to analyse said microbial population.

公开(公告)号：EP3261113A1

公开(公告)日：2017-12-27

申请号：EP17176718.9

申请日：2017-06-19

申请人： Thermo Finnigan LLC

发明人： ZABROUSKOV, Vladimir ,  MULLEN, Christopher

IPC分类号： H01J49/42 ,  H01J49/00

CPC分类号： G01N33/6851 ,  H01J49/0031 ,  H01J49/0072 ,  H01J49/422

摘要： A method for analyzing a sample includes trapping a first ion packet in a first segment of a multi-segment reaction cell; trapping a second ion packet in a second segment of the multi-segment reaction cell; and trapping a third ion packet in a third segment of the multi-segment reaction cell. At least one of the first, second, and third ion packets includes precursor ions, and at least another one of the first, second, and third ion packets includes reagent ions. The method further includes mixing the first, second, and third ion packets within the reaction cell to cause a reaction between the precursor ions and the reagent ions to form product ions.

摘要翻译： 用于分析样品的方法包括将多个第一离子包捕获在多片段反应池的第一区段中; 捕获多段反应单元的第二段中的第二离子包; 以及捕获多段反应单元的第三段中的第三离子包。 第一，第二和第三离子包中的至少一个包括前体离子，并且第一，第二和第三离子包中的至少另一个包括试剂离子。 该方法还包括在反应池内混合第一，第二和第三离子包以引起前体离子和试剂离子之间的反应以形成产物离子。

公开(公告)号：EP2850435B1

公开(公告)日：2017-08-23

申请号：EP13725191.4

申请日：2013-05-16

申请人： Micromass UK Limited

发明人： EASTWOOD, Martin

IPC分类号： G01N33/68 ,  G01N33/74

CPC分类号： H01J49/0036 ,  G01N33/6851 ,  G01N33/743 ,  H01J49/04

公开(公告)号：EP3186640A1

公开(公告)日：2017-07-05

申请号：EP15760235.0

申请日：2015-08-27

申请人： MAP IP HOLDING LIMITED

发明人： ILES, Raymond Kruse ,  ILES, Jason Kruse ,  ABBAN, Thomas ,  DOCHERTY, Suzanne Margaret Elizabeth ,  NAASE, Mahmoud

IPC分类号： G01N33/68 ,  G01N33/72

CPC分类号： G01N33/6851 ,  G01N33/721

摘要： The method describes rapid screening of whole blood samples, pin prick and blood spot cards, subjected to MALDI—ToF Mass spectrometry. The spectra is generated and compared to those from normal healthy controls. Characteristic spectra are indicative of the presence of a hemoglobinopathy and the method can be used to screen/diagnose all sickle cell diseases, alpha and beta Thalassemias.

摘要翻译： 该方法描述了全血样品，针刺和血斑卡的快速筛选，进行MALDI-ToF质谱分析。 生成光谱并与来自正常健康对照的光谱进行比较。 特征光谱指示存在血红蛋白病并且该方法可用于筛选/诊断所有镰状细胞疾病，α和β地中海贫血。

公开(公告)号：EP3055420A1

公开(公告)日：2016-08-17

申请号：EP14784361.9

申请日：2014-10-09

申请人： Kratos Analytical Limited

发明人： STÜBIGER, Gerald ,  BELGACEM, Omar

IPC分类号： C12Q1/04 ,  G01N33/68 ,  G01N33/92

CPC分类号： C12Q1/04 ,  G01N33/6851 ,  G01N33/92 ,  H01J49/0418

摘要： The present invention is concerned with a method of identifying microbial strains (e.g. from a cell culture), the method comprising; i) a lipid extraction step, comprising extraction of phospholipids from the microbe, suitably with an extraction composition comprising more than 50 vol % MeOH; ii) a sample preparation step, comprising preparation of a MALDI sample incorporating the extracted lipids; iii) a data gathering step, comprising performing MALDI-based mass spectrometry on the MALDI sample, and iv) a microbe identification step, comprising analysis of the mass spectrometry data to characterise or identify the microbial strain. Suitably the method also includes extracting proteins from the microbes and analysing the extracted proteins using MALDI-based mass spectrometry so as to obtain not only lipid m/z data but also protein m/z data.