公开(公告)号：EP0752008A1

公开(公告)日：1997-01-08

申请号：EP95911826.0

申请日：1995-02-17

申请人： AFFYMAX TECHNOLOGIES N.V.

发明人： STEMMER, Willem P.C. ,  CRAMERI, Andreas

IPC分类号： C12N15 ,  A61K38 ,  A61K39 ,  C07K14 ,  C07K16 ,  C12N9 ,  C12P21 ,  C12Q1 ,  C40B40 ,  G01N33

CPC分类号： C40B40/02 ,  C07K14/43595 ,  C07K14/545 ,  C07K16/00 ,  C07K2317/565 ,  C07K2317/622 ,  C12N9/2471 ,  C12N9/86 ,  C12N15/1027 ,  C12N15/1034 ,  C12N15/1037 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Y302/01023 ,  C12Y305/02006 ,  C40B40/08 ,  C40B50/06

摘要： A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.

公开(公告)号：EP0505564B1

公开(公告)日：1997-01-02

申请号：EP92900681.5

申请日：1991-10-23

申请人： THE PERKIN-ELMER CORPORATION

发明人： DOLLINGER, Gavin, D. ,  CUNICO, Robert, L. ,  KUNITANI, Michael, G.

IPC分类号： G01N15/02 ,  G01N30/74

CPC分类号： G01N15/0205 ,  G01N15/04 ,  G01N21/532 ,  G01N30/74 ,  G01N33/68 ,  G01N2021/4726 ,  Y10T436/25375

摘要： A high angle light scattering detector using classical Rayleigh scattering. A high intensity arc light source, filtered to leave only one wavelength illuminates a flow cell. Through the flow cell, very small particles such as biological proteins flow in solution after separation by HPLC or some other means. A UV detector generates data regarding the weight concentration of the eluting particles and a scattered light detector collecting scattered light at angles of approximately 90° generates a scattered light signal. The incident light intensity is also measured. The average molecular weight is then computed using the scattered and incident light data, the weight concentration data and a simplified mathematical relationship from which the size factor P and the viral coefficients have been eliminated.

公开(公告)号：EP0749477A1

公开(公告)日：1996-12-27

申请号：EP95912654.0

申请日：1995-03-01

申请人： THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS

发明人： GUDKOV, Andrei ,  KAZAROV, Alexander ,  MAZO, Ilya ,  RONINSON, Igor B.

IPC分类号： C12N15 ,  A61K31 ,  A61K48 ,  A61P35 ,  C07H21 ,  C07K14 ,  C12N5 ,  C12N9 ,  C12P21 ,  G01N33 ,  A61K38 ,  C12R1

CPC分类号： C12N9/90 ,  A61K38/00 ,  C07K14/4703 ,  C12N15/1034 ,  C12N15/113 ,  C12N15/1137 ,  C12N15/1138 ,  C12N15/67 ,  C12N2310/18 ,  C12N2310/321 ,  C12Y599/01003 ,  G01N33/574 ,  G01N2800/44

摘要： The invention provides genetic suppressor elements that confer the transformed phenotype of malignant mammalian cells upon untransformed cells, methods for identifying and obtaining such elements, methods for isolating and identifying genes corresponding to such elements, and methods of using such elements. The invention also provides genes corresponding to the GSEs of the invention.

公开(公告)号：EP0745132A1

公开(公告)日：1996-12-04

申请号：EP95909541.0

申请日：1995-02-08

申请人： ZymoGenetics, Inc.

发明人： HOLLY, Richard, D. ,  BURKHEAD, Steven, K.

IPC分类号： C07K14 ,  C12N5 ,  G01N33 ,  A61K35

CPC分类号： G01N33/502 ,  A61K2035/124 ,  C07K14/47 ,  C07K14/705 ,  C07K14/715 ,  C12N5/0642 ,  C12N2501/145 ,  C12N2501/22 ,  G01N33/5005 ,  G01N33/5008

摘要： Methods for obtaining cells that produce a ligand for an orphan receptor and methods for preparing polynucleotide molecules that encode ligands for orphan receptors are disclosed. The methods utilize growth factor-dependent parent cells that are transfected with a DNA construct encoding an orphan receptor. The transfected cells are exposed to mutagenizing conditions, and the mutagenized cells are cultured under conditions in which cell survival is dependent upon autocrine growth factor production. Progeny cells are recovered and screened to identify those that produce a ligand for the orphan receptor. Polynucleotide molecules encoding the ligand can be prepared from the identified cells.

公开(公告)号：EP0740549A1

公开(公告)日：1996-11-06

申请号：EP95908606.0

申请日：1995-01-23

申请人： JAVITT, Jonathan C. ,  McDONNELL, Peter ,  JAVITT, Norman B.

发明人： JAVITT, Jonathan C. ,  McDONNELL, Peter ,  JAVITT, Norman B.

IPC分类号： A61K45 ,  A61K31 ,  A61P27

CPC分类号： A61K31/40 ,  A61K31/00

摘要： A method of inhibiting corneal scarring or haze, especially produced as a result of laser surgery of a patient, comprises administering to the patient an amount of a compound, e.g., cis-hydroxyproline, which modifies the structure of collagen.

摘要翻译： 特别是由于患者的激光手术而产生的抑制角膜瘢痕形成或浑浊的方法包括给患者施用一定量的改变胶原结构的化合物，例如顺式羟脯氨酸。

公开(公告)号：EP0738156A1

公开(公告)日：1996-10-23

申请号：EP95904993.0

申请日：1994-12-22

申请人： Hamilton Civic Hospitals Research Development, Inc.

发明人： WEITZ, Jeffrey I. ,  HIRSH, Jack

IPC分类号： C12N9 ,  A61K38 ,  A61K39 ,  A61P7 ,  A61P9 ,  A61P43 ,  C07K14 ,  C07K16

CPC分类号： C12N9/6429 ,  A61K38/363 ,  A61K38/4833 ,  C07K14/75 ,  C07K14/811 ,  C07K16/36 ,  C07K16/40 ,  C12Y304/21005

摘要： Thrombus or clot formation and accretion are inhibited by administering thrombin-displacing substances to a patient. The thrombin-displacing substances comprise either thrombin analogs which bind to the thrombin-binding site on fibrin or fibrin analogs which bind to a fibrin-binding site on thrombin. By displacing the thrombin from clot or thrombus, the thrombin is released into circulation where it is inactivated by endogenous anti-proteinases. The fibrin analogs which bind to the fibrin-binding site on thrombin may be linked to a second binding moiety which binds to and/or inactivates the catalytic site and/or exosite I on thrombin to further inhibit thrombosis. The ability of test substances to displace thrombin is measured by exposure of the substance to immobilized thrombin-fibrin complex.

公开(公告)号：EP0737746A2

公开(公告)日：1996-10-16

申请号：EP95203326.4

申请日：1990-11-30

申请人： PHARMING B.V.

发明人： Heyneker, Herbert L. ,  Deboer, Herman A. ,  Strijker, Rein ,  Platenburg, Gerard ,  Lee, Sang He

IPC分类号： C12N15/00 ,  A01K67/027 ,  A23C9/00

CPC分类号： A01K67/0278 ,  A01K67/0275 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/101 ,  A01K2227/105 ,  A01K2267/01 ,  A23C9/20 ,  C07K14/4732 ,  C07K14/765 ,  C07K14/79 ,  C12N9/6464 ,  C12N15/8509 ,  C12Y304/21069

摘要： The invention provides a transgenic bovine species capable of producing a recombinant polypeptide in at least one cell type of said animal. Other aspects include transgenes for the production of such animals, milk resulting therefrom and the use of such milk and heterologous polypeptides contained within such milk.

摘要翻译： 本发明提供能够在所述动物的至少一种细胞类型中产生重组多肽的转基因牛物种。 其他方面包括用于生产这样的动物的转基因，由此产生的乳和使用这样的牛奶中所含的这种乳和异源多肽。

公开(公告)号：EP0737068A1

公开(公告)日：1996-10-16

申请号：EP95905361.0

申请日：1994-12-14

申请人： ZymoGenetics, Inc.

发明人： LABROO, Virender, M. ,  PIGGOTT, James, R.

IPC分类号： C07D311 ,  A61K31 ,  A61P29 ,  A61P37

CPC分类号： A61K31/40 ,  A61K31/35 ,  A61K31/445 ,  Y10S514/861 ,  Y10S514/863

摘要： Methods and pharmaceutical compositions for the treatment of dermatitis are disclosed. 2,3-diaryl-1-benzopyrans and their pharmaceutically acceptable salts are formulated into medicaments, including oral and topical medicaments, which are administered to a patient suffering from dermatitis. The methods and compositions are particularly useful in the treatment of conditions characterized by hyperproliferation of keratinocytes, such as psoriasis.

公开(公告)号：EP0553248B1

公开(公告)日：1996-09-18

申请号：EP91919667.5

申请日：1991-10-16

申请人： OCTAMER, INC.

发明人： KUN, Ernest ,  MENDELEYEV, Jerome

IPC分类号： A61K31/365 ,  C07D311/14 ,  C07D311/16 ,  C07D311/18 ,  C07D311/54

CPC分类号： A61K31/37 ,  C07D311/14 ,  Y10S514/934

摘要： Unsubstituted or substituted 5-iodo-6-amino-1,2-benzopyrones and their analogs are potent, selective and non-toxic inhibitors and suppressants of cancer growth and viral infections in a mammalian host. The compounds are particularly useful for treatment and suppression of tumors and viruses associated with AIDS, herpetic episodes and cytomegaloviral infections.

公开(公告)号：EP0730568A1

公开(公告)日：1996-09-11

申请号：EP95904094.0

申请日：1994-11-17

申请人： MEGABIOS CORPORATION

发明人： HEATH, Timothy, D. ,  SOLODIN, Igor

IPC分类号： C12N15 ,  A61K9 ,  A61K47 ,  A61K48 ,  C07D295 ,  C07H21

CPC分类号： C07D295/088 ,  A61K9/1272 ,  C12N15/88

摘要： Novel, heterocyclic cationic amphiphile and compounds thereof are prepared that are degraded in vivo. Liposomes are produced from the cations that are used as carriers for delivering macromolecules intracellularly and may be targeted to a specific cell type.