公开(公告)号：EP0973038B1

公开(公告)日：2006-08-23

申请号：EP99112316.7

申请日：1999-06-26

申请人： Wako Pure Chemical Industries, Ltd.

发明人： Kato, Ryoji ,  Maruyama, Masayuki ,  Nakamura, Kenji ,  Shimizu, Kayoko ,  Satomura, Shinji

IPC分类号： G01N33/78 ,  G01N33/566

CPC分类号： G01N33/78 ,  G01N33/574 ,  G01N2333/71

公开(公告)号：EP1614477A1

公开(公告)日：2006-01-11

申请号：EP05017769.0

申请日：2000-09-28

申请人： WAKO PURE CHEMICAL INDUSTRIES, LTD

发明人： Washizu, Masao ,  Kawabata, Tomohisa

IPC分类号： B03C5/00 ,  B03C5/02

CPC分类号： B03C5/005

摘要： The present invention has an object of providing a method by which two kinds or more of molecules can be separated each other by using dielectrophoretic forces. The present invention comprises two methods. The first method is a method comprising forming a complex substance of a "specific molecule" containing in a sample, and a "substance capable of changing dielectrophoretic properties of the specific molecule", which binds to the "specific molecule" contained therein, and thereby separating the complex substance and the molecules other than the specific molecule in the sample from each other. The second method is a method comprising placing a solution in which two kinds or more of molecules are dissolved under a strong electric field strength, that is, under a nonuniform electric field having an electric field strength of 500 KV/m or higher, by using dielectrophoretic forces.

摘要翻译： 本发明的目的是提供一种通过使用介电电泳力将两种以上的分子彼此分离的方法。 本发明包括两种方法。 第一种方法是包含形成包含在样品中的“特定分子”的复合物质和与其中所含的“特定分子”结合的“能够改变特定分子的介电电泳性质的物质”的方法，从而 将样品中的特定分子之外的复合物质和分子彼此分离。 第二种方法是一种方法，其包括在强电场强度（即，在500KV / m以上的电场强度不均匀的电场下）放置其中两种或更多种分子溶解的溶液，通过使用 介电电泳力。

公开(公告)号：EP1588751A1

公开(公告)日：2005-10-26

申请号：EP03812342.8

申请日：2003-11-28

申请人： Wako Pure Chemical Industries, Ltd.

发明人： DAVID, Godwin Cork ,  INOUE, Kunihisa

IPC分类号： B01D17/00 ,  B01D61/00 ,  B01D71/36 ,  C07C231/24 ,  C07C231/02 ,  C07C231/08 ,  C07B63/00

CPC分类号： B01D17/085 ,  B01D61/18 ,  B01D63/00 ,  C40B40/04 ,  C40B50/08

摘要： To provide a water separation device and a water separation method in which an object in a water dispersion organic solvent is not adsorbed and neither contaminated, and water can be separated and removed in a short period of time irrespective of specific gravity of an organic layer in the water separation organic solvent, characterized in that a water separation membrane is provided on a rising surface between a lower end and an upper end of a tubular member, and a water dispersion organic solvent is filtrated by the water separation membrane to thereby separate water to cause the organic solvent to pass through.

摘要翻译： 提供一种水分离装置，并且其中水的分离方法中的水分散体到对象未吸附的有机溶剂和既不污染，并且水可以在有机层的比重来分离和去除在很短的时间周期而不管 水分离的有机溶剂，在其特点做设置在下端之间在管状部件的上端的上升表面的水的分离膜，以及水分散体的有机溶剂是由水分离膜，从而分离水至过滤 使有机溶剂通过。

公开(公告)号：EP1577280A1

公开(公告)日：2005-09-21

申请号：EP03814536.3

申请日：2003-11-07

申请人： Wako Pure Chemical Industries, Ltd.

发明人： ITO, Nobuhiro ,  MAESAWA, Tsuneaki ,  MUTO, Kazushige ,  HIROTA, Kosaku ,  SAJIKI, Hironao

IPC分类号： C07B59/00 ,  C07C29/00 ,  C07C31/02 ,  C07C35/08 ,  C07C35/29 ,  C07C35/37 ,  C07C45/00 ,  C07C49/04 ,  C07C49/08 ,  C07C49/433 ,  C07C49/453 ,  C07C51/00 ,  C07C53/10 ,  C07C53/124 ,  C07C57/04

CPC分类号： C07B59/00 ,  C07B2200/05 ,  C07C29/00 ,  C07C45/00 ,  C07C2601/14 ,  C07C2602/42 ,  C07C2603/68 ,  C07C31/125 ,  C07C35/08 ,  C07C35/29 ,  C07C35/37

摘要： The present invention relates to a method for deuteration of a compound represented by the general formula [1]:

        R 1 ―X―R 2      [1]

   wherein, R 1 represents an alkyl group or an aralkyl group, which may have a carbon-carbon double bond and/or triple bond; R 2 represents an alkyl group which may have a carbon-carbon double bond and/or triple bond, an aryl group, an aralkyl group, an alkoxy group, an aryloxy group or a hydroxyl group; X represents a carbonyl group or a hydroxylmethylene group; R 1 and R 2 may form an alicyclic ring together with a carbon atom contained in X; provided that R 2 represents an alkyl group which may have a carbon-carbon double bond and/or triple bond, an aryl group or an aralkyl group when X is a hydroxylmethylene group,
comprising reacting the compound represented by the general formula [1] with a heavy hydrogen source in the co-presence of an activated catalyst selected from a palladium catalyst, a platinum catalyst, a rhodium catalyst, a ruthenium catalyst, a nickel catalyst and a cobalt catalyst. The method of the present invention can significantly improve working environment because the deuteration, which has been conventionally carried out under severe conditions such as basic condition, can be carried out under neutral condition. Further, even when the compound represented by the general formula [1] is one having a carbon-carbon double bond or triple bond, the method for deuteration of the present invention enables to efficiently carry out objective deuteration without reduction of said double bond or triple bond.

摘要翻译： 本发明涉及通式[1]所示化合物的重氢化方法：R 1 -X-R 2 [1]其中，R 1表示可具有碳 - 碳双键的烷基或芳烷基 和/或三键; R2表示可具有碳 - 碳双键和/或三键的烷基，芳基，芳烷基，烷氧基，芳氧基或羟基; X代表羰基或羟亚甲基; R1和R2可以与X中含有的碳原子一起形成脂环; 条件是R2表示可具有碳 - 碳双键和/或三键的烷基，当X为羟基亚甲基时为芳基或芳烷基，所述方法包括使通式[1]代表的化合物与 在选自钯催化剂，铂催化剂，铑催化剂，钌催化剂，镍催化剂和钴催化剂的活化催化剂的共存下重氢源。 本发明的方法可以显着改善工作环境，因为在中性条件下可以在苛刻的条件如碱性条件下执行常规进行的氘化。 此外，即使通式[1]所示的化合物为具有碳 - 碳双键或三键的化合物，本发明的重氢化方法也能够在不使所述双键或三重还原的情况下有效地进行目标重氢化 键。

公开(公告)号：EP1561741A1

公开(公告)日：2005-08-10

申请号：EP03811499.7

申请日：2003-11-07

申请人： Wako Pure Chemical Industries, Ltd.

发明人： ITO, Nobuhiro ,  MAESAWA, Tsuneaki ,  MUTO, Kazushige ,  HIROTA, Kosaku ,  SAJIKI, Hironao

IPC分类号： C07B59/00 ,  C07D209/08 ,  C07D209/20 ,  C07D213/06 ,  C07D231/12 ,  C07D233/58 ,  C07D235/08 ,  C07D239/47 ,  C07D239/54 ,  C07D471/04 ,  C07D473/30 ,  C07D473/34 ,  C07H19/067 ,  C07H19/167

CPC分类号： C07D213/16 ,  C07B59/002 ,  C07D209/20 ,  C07D231/12 ,  C07D233/54 ,  C07D233/56 ,  C07D235/06 ,  C07D239/54 ,  C07D471/04 ,  C07D473/30 ,  C07H19/067 ,  C07H19/167 ,  Y02P20/544

摘要： The present invention relates to a method for deuteration of a heterocyclic ring, which comprises subjecting a compound having a heterocyclic ring to sealed refluxing state in a deuterated solvent in the presence of an activated catalyst selected form a palladium catalyst, a platinum catalyst, a rhodium catalyst, a ruthenium catalyst, a nickel catalyst and a cobalt catalyst. In accordance with a method of the present invention, a hydrogen atom belonging to a heterocyclic ring of a compound having a heterocyclic ring can be very efficiently deuterated because temperature of deuteration reaction can be maintained at higher than boiling point of the solvent.
Further, a method for deuteration of the present invention can be applied widely to deuteration of various compounds having a heterocyclic ring which are liable to decomposition under supercritical conditions or acidic conditions, leading to industrial and efficient deuteration of a compound having a heterocyclic ring.

摘要翻译： 本发明涉及杂环的氘化方法，该方法包括在选自钯催化剂，铂催化剂，铑的活化催化剂存在下，使具有杂环的化合物在氘代溶剂中密封回流 催化剂，钌催化剂，镍催化剂和钴催化剂。 根据本发明的方法，具有杂环的化合物的杂环的氢原子可以非常有效地氘化，因为氘代反应的温度可以保持在溶剂的沸点以上。 此外，本发明的氘化方法可以广泛地应用于具有杂环的各种化合物的猝灭，所述化合物在超临界条件或酸性条件下易于分解，导致工业和高效氘化具有 杂环。

公开(公告)号：EP1548002A1

公开(公告)日：2005-06-29

申请号：EP03808867.0

申请日：2003-08-27

申请人： Wako Pure Chemical Industries, Ltd.

发明人： KIMURA, Takuhiro ,  TANI, Tsutomu ,  MIYAHARA, Reiji

IPC分类号： C07C309/14

CPC分类号： C07C303/22 ,  C07C303/32 ,  C07C309/14

摘要： The present invention relates to a method for efficiently producing an aminoalkylsulfonic acid in an industrial scale, and provides
   "a process for producing an aminoalkylsulfonic acid represented by the general formula [2]:
   wherein R 1 and R 2 are each independently a hydrogen atom, an alkyl group, an aryl group or an aralkyl group; and R 3 and R 4 are each independently a hydrogen atom or an alkyl group,
comprising reacting an aminoalkylsulfonate salt represented by the general formula [1]:
   wherein M is an alkali metal atom, an organic ammonium ion or an ammonium ion; and R 1 to R 4 are the same as described above,
   an aqueous solution thereof, or a solution dissolving any one of them in a water-soluble organic solvent, selected from alcohols having 1 to 3 carbon atoms, carboxylic acids having 2 to 12 carbon atoms and dimethylformamide, with an organic acid; and
   a method of salt exchange for an aminoalkylsulfonate salt represented by the general formula [1']:
   wherein M' is an alkali metal atom, an organic ammonium ion or an ammonium ion; and R 1 and R 4 are the same as described above, comprising reacting an aminoalkylsulfonate salt represented by the above general formula [2] with a hydroxide represented by the general formula [6]:

        M'OH     [6]

   wherein M' is the same as described above,
in an alcohol or water".

摘要翻译： 本发明涉及一种方法，用于有效地在氨基烷基磺酸在工业规模生产，并提供了“一种用于在氨基烷基磺酸由通式A2U代表产生： worin - [R <1>和R <2>各自 unabhängig氢原子的烷基基团的芳基或芳烷基在烷基;和R <3>和R <4>各自unabhängig为氢原子或烷基，该方法包括使向aminoalkylsulfonates盐由通式A1U表示： worin M是碱金属原子，以有机铵离子或铵离子的;且R <1>至R <4>中的相同如上所述，为了wässrige其溶液，或溶解它们中的任何一种的溶液 在水溶性有机溶剂中，选自具有1至3个碳原子，具有2至12个碳原子和二甲基甲酰胺，与有机酸的羧酸醇中;以及用于由所述通式表示的aminoalkylsulfonates盐盐交换的方法1 “T： worin M“是一种碱金属原子的有机铵离子或铵离子; 和R <1>和R <4>是相同的，如上所述，该方法包括使向aminoalkylsulfonates盐与由上述通式表示的A6U氢氧化物上述通式A2U表示：M'OH worin M“是相同的描述 如上所述，在以醇或水”。

公开(公告)号：EP1535889A1

公开(公告)日：2005-06-01

申请号：EP03771263.5

申请日：2003-07-10

申请人： Wako Pure Chemical Industries, Ltd.

发明人： ITO, Nobuhiro ,  MAESAWA, Tsuneaki ,  MUTO, Kazushige ,  HIROTA, Kosaku ,  SAJIKI, Hironao

IPC分类号： C07B59/00 ,  C07C5/00 ,  C07C15/16 ,  C07C37/00 ,  C07C39/04 ,  C07C39/28 ,  C07C51/347 ,  C07C63/06 ,  C07C63/08 ,  C07C209/68 ,  C07C211/46 ,  C07C217/84 ,  C07C315/04 ,  C07C317/14

CPC分类号： C07C5/00 ,  C07B59/00 ,  C07B59/001 ,  C07B2200/05 ,  C07C37/00 ,  C07C51/00 ,  C07C51/347 ,  C07C209/68 ,  C07C315/04 ,  C07C63/08 ,  C07C63/06 ,  C07C317/14 ,  C07C211/46 ,  C07C211/51 ,  C07C39/04 ,  C07C39/28

摘要： The present invention relates to a method for deuteration of a compound having an aromatic ring, using an activated catalyst, and the method comprises reacting a compound having an aromatic ring with heavy hydrogen source in the presence of an activated catalyst selected from a platinum catalyst, a rhodium catalyst, a ruthenium catalyst, a nickel catalyst and a cobalt catalyst.

摘要翻译： 本发明涉及一种使用活化催化剂氘代具有芳环的化合物的方法，该方法包括在活性催化剂存在下使具有芳环的化合物与重氢源反应，所述催化剂选自铂催化剂， 铑催化剂，钌催化剂，镍催化剂和钴催化剂。

公开(公告)号：EP1500934A1

公开(公告)日：2005-01-26

申请号：EP02722738.8

申请日：2002-04-24

申请人： WAKO PURE CHEMICAL INDUSTRIES, LTD

发明人： TAKAHASHI, Hakuo ,  MASUDA, Midori

IPC分类号： G01N33/577 ,  G01N33/569

CPC分类号： C07K16/283 ,  C12Q1/6883 ,  G01N33/6893 ,  G01N2333/70535

摘要： The present invention discloses a method for determining a risk of atherosclerosis or future development of atherosclerotic complications by measuring an amount of a soluble Fcγ receptor IIIa Mφ derived from macrophage (hereafter abbreviated as sFcγRIIIa Mφ ) in a blood sample from patients by using an antibody specifically recognizing FcγRIIIa Mφ , and determining the risk on the basis of the result of the measurement, a kit for measuring an amount of sFcγRIIIa Mφ and a kit for determining a risk of development of atherosclerosis or future development of atherosclerotic complications.
A determination method of the present invention is a non-invasive method with greatly mitigated patient burden compared with a conventional cardiac catheterization method, because it is a method by measuring an amount of sFcγRIIIa Mφ in a blood sample, which is a novel predictive marker of atherosclerosis. Furthermore, the present method can also determine a risk of coming down with atherosclerotic complications in the future for a case of superficially healthy but with progressing atherosclerosis internally.

摘要翻译： 本发明盘松动确定性采矿的方法动脉粥样硬化或动脉粥样硬化并发症的未来发展的风险通过可溶性Fcγ受体IIIa的的量从巨噬细胞衍生的测量在（以下简称为SFC伽马RIIIA ） 从患者的血液样品，通过使用抗体特异性识别的FcγRIIIA ，和确定性采矿的测量中，对于SFC伽马RIIIA 的量的测定试剂盒的结果的基础上的风险和对试剂盒 确定性采矿动脉粥样硬化或动脉粥样硬化并发症的未来发展的发展的风险。 本发明的确定方法是一种非侵入性方法与大大减轻患者的负担与常规的心脏导管插入术的方法相比，由于它是通过在血液伽马SFC RIIIA 的量的测量方法 样品，所有这些是动脉粥样硬化的一种新颖的预测性标记物。 进一步，本发明的方法因此可以确定性矿下来动脉粥样硬化的并发症在未来健康表面上的情况下，但随着内部动脉粥样硬化进展的风险。

公开(公告)号：EP0789279B2

公开(公告)日：2004-12-08

申请号：EP96309141.8

申请日：1996-12-13

申请人： WAKO PURE CHEMICAL INDUSTRIES, LTD

发明人： Urano, Fumiyoshi ,  Fujie, Hirotoshi ,  Oono, Keiji

IPC分类号： G03F7/039 ,  G03F7/004 ,  C08F212/14 ,  C08F12/14 ,  C08F112/14

CPC分类号： G03F7/039 ,  C08F212/14 ,  G03F7/0045 ,  Y10S430/106

公开(公告)号：EP1376126A1

公开(公告)日：2004-01-02

申请号：EP02714427.8

申请日：2002-04-03

申请人： Wako Pure Chemical Industries, Ltd.

发明人： KAWABATA, Tomohisa ,  NAKAMURA, Kenji ,  SATOMURA, Shinji

IPC分类号： G01N33/537 ,  G01N33/532 ,  G01N27/447

CPC分类号： G01N33/532 ,  C12Q1/68 ,  G01N27/44726 ,  C12Q2565/125 ,  C12Q2563/179

摘要： The invention relates to a method for separating a target for measurement utilizing electrophoresis, particularly capillary electrophoresis efficiently in high sensitivity and in a short period of time. It also relates to a method for measuring said target separated by said method for separation. The invention provides a method for separating a target for measurement and a method for measuring said target separated bysaidmethodforseparation, characterizedbyusingasubstance to which is bound a nucleic acid chain labeled by a marker and which has an affinity for said target for measurement.

摘要翻译： 本发明涉及一种利用电泳，特别是高灵敏度和短时间内高效毛细管电泳分离测定靶的方法。 本发明还涉及一种用于分离由所述分离方法分离的所述靶的测量方法。 本发明提供了一种用于分离测量目标的方法以及用于测量所述目标分离的副产物分离方法的方法，其特征在于，其中结合由标记物标记的核酸链的物质，并且其具有对所述靶标进行测量的亲和力。