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107 条记录 (耗时 0.019 秒)

    EFFICIENT PURIFICATION OF ADENOVIRUS
    11.
    发明授权
    EFFICIENT PURIFICATION OF ADENOVIRUS 有权
    高效清洗腺病毒

    公开(公告)号：EP1073721B1

    公开(公告)日：2005-08-31

    申请号：EP99918788.3

    申请日：1999-04-22

    申请人： GenVec, Inc.

    发明人： CARRION, Miguel, E. ,  MENGER, Marilyn ,  KOVESDI, Imre

    IPC分类号： C12N7/02 ,  G01N33/569

    CPC分类号： C07K14/005 ,  B01D15/345 ,  B01D15/363 ,  B01J41/20 ,  C12N7/00 ,  C12N2710/10322 ,  C12N2710/10351 ,  C12Q1/06 ,  G01N30/06 ,  G01N30/96 ,  G01N2030/625 ,  G01N2030/8813 ,  G01N2333/075

    摘要： A method of enriching a solution of an adenovirus comprising applying a mixed solution comprising an adenovirus and at least one undesired type of biomolecule to an anion exchange chromatography resin containing a binding moiety selected from the group consisting of dimethylaminopropyl, dimethylaminobutyl, dimethylaminoisobutyl, and dimethylaminopentyl and eluting the adenovirus from the chromatography resin. Also provided is a method of purifying an adenovirus from adenovirus-infected cells comprising lysing such cells, applying the lysate to a single chromatography resin, eluting the adenovirus from the chromatography resin, and collecting a fraction containing adenovirus that is substantially as pure as triple CsC1 density gradient-purified adenovirus. The present method further provides a method of accurately quantifying the number of adenoviral particles in a solution of adenovirus comprising applying to and eluting from an anion exchange chromatography resin a sample solution of adenovirus, comparing the absorbance of the sample solution of adenovirus and the absorbance of a standard solution of adenovirus, and quantifying the number of adenoviral particles in the sample solution.

    CORONARY ARTERY DISEASE TREATMENT
    12.
    发明公开
    CORONARY ARTERY DISEASE TREATMENT 审中-公开
    冠状动脉疾病治疗

    公开(公告)号：EP1560490A2

    公开(公告)日：2005-08-10

    申请号：EP03768930.4

    申请日：2003-11-14

    申请人： GENVEC, INC. ,  WARNER-LAMBERT COMPANY

    发明人： SAMYN, Margaret ,  YONEHIRO, Grant ,  RASMUSSEN, Henrik, S.

    IPC分类号： A01N63/00

    CPC分类号： C12N15/86 ,  A61K48/00 ,  A61K48/0075 ,  A61K48/0083 ,  C12N2710/10343

    摘要： The invention provides a method of treating coronary artery disease in a human patient comprising directly injecting into an ischemic cardiac muscle, via multiple injections to different points of the cardiac muscle, a dose of a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a replication-deficient adenoviral vector comprising a nucleic acid sequence encoding an angiogenic peptide operably linked to a promoter, whereby the coronary artery disease is treated. Figure 1 is a bar graph that depicts the change in time to at least 1 mm ST-segment depression on exercise electrocardiogram (ECG) from baseline at 12 weeks and 26 weeks post-treatment in accordance with the invention.

    摘要翻译： 本发明提供了一种治疗人类患者的冠状动脉疾病的方法，其包括经由多次注射至心肌的不同点直接注射至缺血性心肌中的一剂药物组合物，所述药物组合物包含药学上可接受的载体和复制缺陷型 腺病毒载体，其包含编码可操作地连接至启动子的血管生成肽的核酸序列，由此治疗冠状动脉疾病。 图1是描绘根据本发明在治疗后12周和26周从运动心电图（ECG）至少1mm ST段压低相对于基线的时间变化的条形图。

    GENE THERAPY FOR TREATING OCULAR-RELATED DISORDERS
    14.
    发明公开
    GENE THERAPY FOR TREATING OCULAR-RELATED DISORDERS 审中-公开
    基因治疗眼睛投诉处理

    公开(公告)号：EP1253948A2

    公开(公告)日：2002-11-06

    申请号：EP01909022.4

    申请日：2001-02-09

    申请人： GENVEC, INC.

    发明人： KOVESDI, Imre ,  BROUGH, Douglas, E. ,  MCVEY, Duncan, L. ,  WEI, Lisa

    IPC分类号： A61K48/00

    CPC分类号： A61K48/0075 ,  A61K48/005 ,  C07K14/71 ,  C07K14/811 ,  C12N15/86 ,  C12N2710/10343

    摘要： The present invention is directed to a method of prophylactically or therapeutically treating an animal for at least one ocular-related disorder, e.g., ocular neovascularization or age-related macular degeneration. The method comprises contacting an ocular cell with an expression vector comprising a nucleic acid sequence encoding an inhibitor of angiogenesis and the same or different nucleic acid sequence encoding a neurotrophic agent. The method also can comprise contacting an ocular cell with different expression vectors, each comprising a nucleic acid sequence encoding an inhibitor of angiogenesis and/or a nucleic acid sequence encoding a neurotrophic agent. In addition, the present invention provides a viral vector comprising a nucleic acid sequence encoding pigment epithelium-derived factor (PEDF) or a therapeutic fragment thereof.

    METHOD FOR ASSESSING THE RELATIVE PURITY OF VIRAL GENE TRANSFER VECTOR STOCKS
    15.
    发明公开
    METHOD FOR ASSESSING THE RELATIVE PURITY OF VIRAL GENE TRANSFER VECTOR STOCKS 审中-公开
    方法，评估投资组合病毒基因转移载体的相对纯度

    公开(公告)号：EP1108069A1

    公开(公告)日：2001-06-20

    申请号：EP99939128.7

    申请日：1999-08-10

    申请人： GENVEC, INC.

    发明人： CARRION, Miguel ,  KOVESDI, Imre

    IPC分类号： C12Q1/70

    CPC分类号： C12Q1/70 ,  C12Q1/6816 ,  C12Q2565/627

    摘要： The present method involves evaluating the purity of a viral gene transfer vector stock by providing an aliquot of the viral gene transfer vector stock and producing charged fragments from the aliquot. The fragments are detected by a detector that produces a sample signal corresponding to the mass-to-charge ratio of the detected fragments. This signal then is compared to a standard signal, whereby the purity of the viral gene transfer vector stock is evaluated.