公开(公告)号：EP1550723A1

公开(公告)日：2005-07-06

申请号：EP03798554.6

申请日：2003-09-30

申请人： KANEKA CORPORATION

发明人： OGAWA, Noriko ,  MIYAMOTO, K., 101, Nikkanhaimu Dai 8 ,  OSAKADA, Fumio ,  MATSUMOTO, Keiji

IPC分类号： C12P7/62 ,  C08G63/90

CPC分类号： C08G63/90 ,  C08G63/06

摘要： The object of this invention is to provide a method for purifying PHA separated from PHA-containing microbial cells in high purity without incurring any serious decrease in molecular weight.
The present invention provides a method for purifying a 3-hydroxyalkanoic acid copolymer produced by a microorganism,
   which comprises treating an aqueous suspension containing the 3-hydroxyalkanoic acid copolymer separated from a microorganism with a hydrogen peroxide while controlling the pH of said aqueous suspension by adding an alkali either continuously or intermittently.

摘要翻译： 本发明的目的是提供一种从高纯度纯化含PHA微生物细胞分离PHA的方法，而不会导致分子量的严重下降。 本发明提供了一种纯化由微生物制备的3-羟基链烷酸共聚物的方法，该方法包括用含有过氧化氢的微生物分离的含有3-羟基链烷酸共聚物的含水悬浮液进行处理，同时控制所述 水悬浮液，通过连续或间歇地加入碱。

公开(公告)号：EP1352958A1

公开(公告)日：2003-10-15

申请号：EP02716318.7

申请日：2002-01-18

申请人： KANEKA CORPORATION

发明人： FUKUCHI, Ken ,  YOKOMIZO, Satoru ,  OSAKADA, Fumio ,  MATSUMOTO, Keiji ,  TAKAGI, Masamichi ,  OTA, Akinori

IPC分类号： C12N15/09 ,  C12N1/19 ,  C12P7/62

CPC分类号： C12N9/93 ,  C12P7/625 ,  C12Y603/02006

摘要： The invention provides a yeast in which a specific gene alone has been disrupted and to which auxotrophy is impared to construct a system for producing an industrially useful substance through gene recombination by utilizing characteristics of a yeast.
In the practice of the invention, a yeast strain in which ADE1 gene has been disrupted is constructed by homologous recombination with a chromosomal DNA by using an ADE1 gene fragment encoding phosphoribosylaminoimidazole succinocarboxamide synthase (EC6.3.2.6) of Candida maltosa. In the practice of the invention, then, this disrupted strain is transformed by a gene expression cassette containing a biodegradable polyester synthase gene and the obtained transformant is cultured to thereby produce biodegradable polyester.

摘要翻译： 本发明提供了一种酵母，其中单独的特定基因已经被破坏并且通过利用酵母的特性通过基因重组构建了用于产生工业上有用物质的制剂的营养缺陷型。 在本发明的实践中，通过使用编码拟南芥假丝酵母的磷酸核糖氨基咪唑琥珀酰胺合成酶（EC6.3.2.6）的ADE1基因片段，通过与染色体DNA的同源重组构建其中ADE1基因被破坏的酵母菌株 。 在本发明的实践中，通过含有可生物降解的聚酯合酶基因的基因表达盒转化该破坏的菌株，培养得到的转化体，从而生成可生物降解的聚酯。

公开(公告)号：EP0987332A1

公开(公告)日：2000-03-22

申请号：EP98914111.4

申请日：1998-04-20

申请人： Kaneka Corporation

发明人： YAMADA, Yukio ,  IWASAKI, Akira ,  KIZAKI, Noriyuki ,  MATSUMOTO, Keiji ,  IKENAKA, Yasuhiro ,  OGURA, Masahiro ,  HASEGAWA, Junzo

IPC分类号： C12P13/04 ,  C12N15/54

CPC分类号： C12P13/001 ,  C12N9/1096

摘要： A process for efficiently producing optically active amino compounds, mainly (R)-amino compounds, at a low cost by means of a microbial enzyme; a polypeptide suitable for use in the production process and having a stereoselective transaminase activity; and a DNA coding for the polypeptide. The process is characterized by comprising causing a transaminase to act on a ketone compound as an amino acceptor in the presence of a primary amine as an amino donor to stereoselectively transfer the amino group to thus obtain an optically active amino compound. The DNA contains a base sequence coding for a polypeptide having a stereoselective transaminase activity. The polypeptide can be obtained by incubating a microorganism belonging to the genus Arthrobacter .

摘要翻译： 一种用于有效地制造光学活性氨基化合物，主要（R）的氨基化合物，在通过微生物酶的手段低成本过程; 适合于在生产过程中使用，并且具有立体选择性转氨酶活性的多肽; 和DNA编码的多肽。 该过程由包括使转氨酶在伯胺的存在下在酮化合物作为氨基受体的作为氨基供体的立体选择性地转移的氨基，得到因而光学活性氨基化合物为特征的。 的DNA含有的碱基序列编码具有立体选择性转氨酶活性的多肽。 所述多肽可通过温育属于节杆菌属的微生物来获得。

公开(公告)号：EP1609868B1

公开(公告)日：2015-11-11

申请号：EP04703505.0

申请日：2004-01-20

申请人： Kaneka Corporation

发明人： YANAGITA, Yoshifumi 1-701, Saususukuea ,  OGAWA, Noriko ,  UEDA, Yasuyoshi ,  OSAKADA, Fumio ,  MATSUMOTO, Keiji

IPC分类号： C12P7/62 ,  C08G63/89

CPC分类号： C12P7/625

公开(公告)号：EP2856862A1

公开(公告)日：2015-04-08

申请号：EP13796736.0

申请日：2013-05-31

申请人： Kaneka Corporation

发明人： NAGIRA, Yozo ,  IMAI, Ryozo ,  SASAKI, Kentaro ,  TAOKA, Naoaki ,  MATSUMOTO, Keiji ,  NOGAWA, Masahiro ,  KOJIMA, Mineo

IPC分类号： A01H1/00 ,  C12N15/09

CPC分类号： C12N15/8205

摘要： This invention provides a method of plant transformation via Agrobacterium comprising inoculating and infecting a plant with an Agrobacterium carrying a foreign-gene-containing vector under application of a plant growth-inhibiting hormone. This invention also provides a method of plant transformation via Agrobacterium comprising transforming a plant with an Agrobacterium carrying a vector comprising a T-DNA region in which a foreign gene is contained but no selectable marker gene is contained.

摘要翻译： 本发明提供了通过农杆菌转化植物的方法，包括在施用植物生长抑制激素的情况下用携带含外源基因的载体的农杆菌接种和感染植物。 本发明还提供了通过农杆菌转化植物的方法，包括用携带载体的农杆菌转化植物，所述载体包含含有外源基因但不含选择标记基因的T-DNA区域。

公开(公告)号：EP1550723B1

公开(公告)日：2009-06-17

申请号：EP03798554.6

申请日：2003-09-30

申请人： KANEKA CORPORATION

发明人： OGAWA, Noriko ,  MIYAMOTO, Kenji 101, Nikkanhaimu Dai 8 ,  OSAKADA, Fumio ,  MATSUMOTO, Keiji

IPC分类号： C12P7/62 ,  C08G63/90 ,  C12R1/01

CPC分类号： C08G63/90 ,  C08G63/06

摘要： It is intended to provide a method of purifying PHA, which is separated from PHA-containing microbial cells, at a high purity without causing a considerable decrease in its molecular weight. Namely, a method of purifying a 3-hydroxyalkanoic acid copolymer produced by a microorganism wherein a treatment with hydrogen peroxide is carried out while continuously or intermittently adding an alkali to an aqueous suspension containing the 3-hydroxyalkanoic acid copolymer separated from the microorganism to thereby control the pH value of the aqueous suspension.

公开(公告)号：EP1609868A1

公开(公告)日：2005-12-28

申请号：EP04703505.0

申请日：2004-01-20

申请人： KANEKA CORPORATION

发明人： YANAGITA, Yoshifumi 1-701, Saususukuea ,  OGAWA, Noriko ,  UEDA, Yasuyoshi ,  OSAKADA, Fumio ,  MATSUMOTO, Keiji

IPC分类号： C12P7/62

CPC分类号： C12P7/625

摘要： The present invention has an object to provide a method for separating and purifying a PHA without causing a serious decrease of the molecular weight to obtain a highly pure PHA in a high yield, which comprises efficiently removing cell components other than PHA particles from a cultured PHA-containing microbial cell. Another object of the present invention is to provide a method for obtaining an agglomerate of PHA particles.
The method for recovering a PHA according to the present invention is a method which comprises efficiently disrupting a cell to recover the PHA by carrying out a physical disruption treatment and an alkali addition at low temperature for an aqueous suspension of the PHA-containing microbial cell, and then treating the PHA with an enzyme and/or a surfactant. Moreover, the particle diameter of the PHA may be enlarged by suspending the PHA in a hydrophilic solvent and/or water, and stirring at a temperature equal to or below the boiling point of said suspension, to agglomerate said PHA.

摘要翻译： 本发明的目的是提供一种分离纯化PHA的方法，而不会导致分子量的严重降低以获得高产率的高纯度PHA，其包括从培养的PHA有效地除去PHA颗粒以外的细胞成分 含微生物细胞。 本发明的另一个目的是提供一种获得PHA颗粒附聚物的方法。 根据本发明的用于回收PHA的方法是一种方法，其包括通过在低温下进行物理破坏处理和碱添加来有效地破坏细胞以恢复PHA，以获得PHA的水性悬浮液， 含有微生物细胞，然后用酶和/或表面活性剂处理PHA。 此外，通过将PHA悬浮在亲水溶剂和/或水中并在等于或低于所述悬浮液沸点的温度下搅拌以使所述PHA聚集，可以扩大PHA的粒径。

公开(公告)号：EP1500705A1

公开(公告)日：2005-01-26

申请号：EP03727987.4

申请日：2003-04-25

申请人： KANEKA CORPORATION

发明人： MIYAMOTO, Kenji, 101, Nikkanhaimu Dai8 ,  OGAWA, Noriko ,  OSAKADA, Fumio ,  MATSUMOTO, Keiji

IPC分类号： C12P7/62 ,  C12N15/09

CPC分类号： C12N1/066 ,  C12P7/625

摘要： The invention aims at providing a method of producing a poly-3-hydroxyalkanoic acid,
   which comprises carrying out a physical disruption treatment of a suspension of poly-3-hydroxyalkanoic acid-containing microbial cells with adding an alkali thereto either continuously or intermittently and, thereafter, separating the poly-3-hydroxyalkanoic acid.

摘要翻译： 本发明的目的在于提供一种生产聚-3-羟基链烷酸的方法，其包括对连续或间歇加入碱的含有聚-3-羟基链烷酸的微生物细胞的悬浮液进行物理破坏处理， 然后分离出聚-3-羟基链烷酸。

公开(公告)号：EP1493811A1

公开(公告)日：2005-01-05

申请号：EP03715776.5

申请日：2003-04-08

申请人： KANEKA CORPORATION

发明人： NAGAOKA, Tetsuya ,  YOKOMIZO, Satoru ,  MIYAMOTO, Kenji, 101, Nikkanhaimu Dai8 ,  OSAKADA, Fumio ,  MATSUMOTO, Keiji ,  TAKAGI, Masamichi ,  OTA, Akinori

IPC分类号： C12N15/11 ,  C12N1/19 ,  C12P7/62

CPC分类号： C07K14/4716 ,  C12N9/1025 ,  C12N15/815

摘要： The present invention provides a production method of a copolymeric polyester which comprises culturing an ACT1 gene promoter shown under SEQ ID NO:9, a GAP3 gene promoter shown under SEQ ID NO:10; a PMA1 gene promoter shown under SEQ ID NO:11, and, a TEF1 gene promoter shown under SEQ ID NO:12; a plasmid which contains the gene expression unit comprising said promoter; a transformed cell as resulting from transformation of the said plasmid; and said transformed cell.

公开(公告)号：EP1283266A1

公开(公告)日：2003-02-12

申请号：EP01930202.5

申请日：2001-05-18

申请人： KANEKA CORPORATION

发明人： YOKOMIZO, Satoru ,  FUKUCHI, Takeshi ,  OSAKADA, Fumio ,  MATSUMOTO, Keiji ,  TAKAGI, Masamichi ,  OHTA, Akinori

IPC分类号： C12N15/52

CPC分类号： C12N9/93 ,  C12N9/88 ,  C12N15/52 ,  C12P7/625

摘要： The present invention relates to a gene coding for a copolyester-synthesizing enzyme, a microorganism which utilizes said gene for the fermentative synthesis of a polyester, and a method of producing a polyester with the aid of said microorganism. More particularly, the present invention relates to a gene which functions in a host organism and is related to synthesize, by an enzyme, a plastic-like polymer degradable under the action of microorganisms in the natural environment (the soil, river or sea), a transformant derived from said host organism by transformation with said gene and having an improved ability to fermentatively synthesize a plastic-like polymer, and a method of producing a copolyester with the aid of said transformant.

摘要翻译： 本发明涉及编码共聚酯合成酶的基因，利用所述基因发酵合成聚酯的微生物，以及借助所述微生物生产聚酯的方法。 更具体地说，本发明涉及在宿主生物中起作用并与酶合成在天然环境（土壤，河流或海洋）中微生物作用下可降解的塑料状聚合物的基因， 通过与所述基因转化并具有改进的发酵合成塑料状聚合物的能力的所述宿主生物体的转化体，以及借助所述转化体生产共聚酯的方法。