公开(公告)号：EP1527199B1

公开(公告)日：2010-07-07

申请号：EP03746704.0

申请日：2003-04-11

申请人： Genzyme Corporation

发明人： SHUBER, Anthony, P.

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/686 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/154 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2523/125 ,  C12Q2549/119 ,  C12Q2525/149

公开(公告)号：EP1497447A4

公开(公告)日：2006-09-20

申请号：EP03721677

申请日：2003-04-14

申请人： UNIV MISSOURI

发明人： HUANG TIM HUI-MING ,  SHI HUIDONG

IPC分类号： G01N33/53 ,  C12M1/00 ,  C12M1/34 ,  C12N15/09 ,  C12Q1/02 ,  C12Q1/68 ,  G01N37/00 ,  C12Q1/00 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q2565/501 ,  C12Q2525/143 ,  C12Q2525/149 ,  C12Q2521/331

摘要： Novel methods are herein provided for high-throughput, dual analysis of DNA methylation and gene expression, and triple analysis of DNA methylation, gene expression and gene-associated histone acetylation in cancer cells using arrayed expressed CpG island sequence tags (ECISTs). ECISTs correspond to genomic DNA fragments comprising GC-rich segments along with promoter and/or exon (e.g., first exon) portions of genes. The GC-rich segments are useful for screening hypermethylated CpG sites in cancer cells, while the corresponding promoter and exon-containing portions are useful for determining corresponding transcript levels and assessing histone acetylation. Also provided are high-throughput methods for either confirming methylation-dependent gene silencing, or identifying therapeutically effective demethylating agents, using the ECIST array panels to identify hypermethylated loci, and measure expression levels thereof after cellular exposure to demethylating agents. Further provided are high-throughput methods for distinguishing between direct (primary) demethylation-dependent gene up-regulation, and indirect (secondary) demethylation-dependent up-regulation within apparent epigenetic cascades.

公开(公告)号：EP0931160A4

公开(公告)日：2004-10-06

申请号：EP96931558

申请日：1996-09-13

申请人： GARVIN ALEX M

发明人： GARVIN ALEX M

IPC分类号： C12Q1/68 ,  C12P19/34 ,  A61K38/00

CPC分类号： C12Q1/68 ,  C12Q1/6804 ,  C12Q2531/143 ,  C12Q2525/149

摘要： A process for detecting protein altering mutations in genes. Coding sequence of the gene is PCR amplified with a 5' primer that contains at its 5' end a polymerase binding site, a translation initiation site, and an in frame sequence coding for a peptide tag, followed by the in frame 5' end of the test sequence. After PCR amplification of the test sequence, the PCR product is used as template to make mRNA in an in vitro transcription reaction using an RNA polymerase that recognizes the polymerase binding site incorporated into the 5' PCR primer. The mRNA is then used as template to make protein in an in vitro translation reaction. The protein encoded by the test sequence has at its amino terminus a peptide tag that can be used to either detect the protein, or to purify the protein for further analysis.

公开(公告)号：EP1062367A1

公开(公告)日：2000-12-27

申请号：EP99914694.7

申请日：1999-02-22

申请人： Johnson & Johnson Research Pty Limited

发明人： TODD, Alison ,  FUERY, Caroline, J. ,  CAIRNS, Murray

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/686 ,  C12Q1/6816 ,  C12Q1/6823 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/6867 ,  C12Q2545/114 ,  C12Q2537/143 ,  C12Q2521/337 ,  C12Q2525/149 ,  C12Q2525/161 ,  C12Q2563/125 ,  C12Q2531/125 ,  C12Q2531/113

摘要： This application provides methods of detecting and quantitatively determining a target nucleic acid sequence in a sample, which comprise contacting the sample with a primer and a zymogene which encodes, but which itself is the anti-sense sequence of, a catalytic nucleic acid sequence, so that when the target is present, a single amplified nucleic acid molecule is produced which comprises the sequences of both the target and catalytic molecules. This invention further provides a method of simultaneously detecting the presence of a plurality of target nucleic acid sequences in a sample. Finally, this invention provides molecules and kits for practicing the instant methods.

公开(公告)号：EP2203567B1

公开(公告)日：2011-05-11

申请号：EP08839669.2

申请日：2008-10-16

申请人： Roche Diagnostics GmbH ,  F. Hoffmann-La Roche AG

发明人： HIGUCHI, Russell, Gene ,  ERLICH, Henry A. ,  BENTLEY, Gordon

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C12Q1/6855 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2563/179 ,  C12Q2525/149 ,  C12Q2565/537

摘要： The invention provides methods and reagent for performing full, multi-locus HLA genotyping for multiple individuals in a single sequencing run using clonal sequencing.

公开(公告)号：EP1732021A4

公开(公告)日：2009-07-29

申请号：EP05721340

申请日：2005-03-23

申请人： BIO THINK TANK CO LTD

发明人： MORISHITA SHINICHI ,  YAMADA TOMOYUKI ,  NAITO YUKI

IPC分类号： C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G06F17/30 ,  G06F19/20 ,  G06F19/22

CPC分类号： G06F19/20 ,  C12Q1/6869 ,  G06F19/22 ,  C12Q2525/149

摘要： It is intended to efficiently determine a base sequence specifically appearing in an expression gene. For this, providing that the expression gene consists of exons (301)...(306) and especially that exon (301) is united with exon (302) and exon (302) with exon (303), an aggregate of base sequences (401) (403) being a union of exon base sequences (301)...(305) and a boundary base sequence obtained by uniting together base sequences(404) and (405) and base sequences (406) and (407) respectively existing over boundaries between exon (301) and exon (302) and between exon (302) and exon (303) is formed, and the aggregate is searched. If a base sequence is one specifically appearing in the expression gene, the number of search results is 1 and otherwise, the number is plural.

公开(公告)号：EP0931160B1

公开(公告)日：2008-12-17

申请号：EP96931558.9

申请日：1996-09-13

申请人： Garvin, Alex M.

发明人： Garvin, Alex M.

IPC分类号： C12P19/34 ,  G01N33/543 ,  C12Q1/68

CPC分类号： C12Q1/68 ,  C12Q1/6804 ,  C12Q2531/143 ,  C12Q2525/149

摘要： A process for detecting protein altering mutations in genes. Coding sequence of the gene is PCR amplified with a 5' primer that contains at its 5' end a polymerase binding site, a translation initiation site, and an in frame sequence coding for a peptide tag, followed by the in frame 5' end of the test sequence. After PCR amplification of the test sequence, the PCR product is used as template to make mRNA in an in vitro transcription reaction using an RNA polymerase that recognizes the polymerase binding site incorporated into the 5' PCR primer. The mRNA is then used as template to make protein in an in vitro translation reaction. The protein encoded by the test sequence has at its amino terminus a peptide tag that can be used to either detect the protein, or to purify the protein for further analysis.

公开(公告)号：EP1490511A4

公开(公告)日：2007-06-06

申请号：EP02786493

申请日：2002-10-24

申请人： SENTION INC

发明人： SLEPNEV VLADMIR I

IPC分类号： G01N27/62 ,  C07H21/02 ,  C12N20060101 ,  C12N15/09 ,  C12P19/34 ,  C12Q1/68 ,  G01N21/78 ,  G01N27/447 ,  G01N30/88 ,  G01N33/48 ,  G01N33/50 ,  G01N33/58

CPC分类号： C12Q1/6809 ,  C12Q2525/161 ,  C12Q2525/149 ,  C12Q2525/173

摘要： The invention provides compositions, methods and systems for dynamic transcription profiling of two or more samples. The method of the invention comprises the uses of sample-specific primers for cDNA synthesis and for subsequent amplification of the synthesized cDNAs. The levels of abundance of genes are compared between samples for the identification of differentially expressed genes.

公开(公告)号：EP1527199A2

公开(公告)日：2005-05-04

申请号：EP03746704.0

申请日：2003-04-11

申请人： Exact Sciences Corporation

发明人： SHUBER, Anthony, P.

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/686 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/154 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2523/125 ,  C12Q2549/119 ,  C12Q2525/149

摘要： The invention provides amplification-based methods for detecting hypermethylated or hypomethylated nucleic acid in heterogeneous biological samples, e.g., stool. A screening procedure based on the detection of hypermethylation, preferably at multiples genes, provides a means for detecting various diseases, e.g., colorectal cancer. By using chimeric primers that contain a 5' non-specific portion, the specificity and efficiency of the nucleic acid amplification is improved. Methods of the invention are especially useful in detection of rare events in a heterogeneous sample.

公开(公告)号：EP1497447A2

公开(公告)日：2005-01-19

申请号：EP03721677.7

申请日：2003-04-14

申请人： THE CURATORS OF THE UNIVERSITY OF MISSOURI

发明人： HUANG, Tim, Hui-Ming ,  SHI, Huidong

IPC分类号： C12Q1/00 ,  C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q2565/501 ,  C12Q2525/143 ,  C12Q2525/149 ,  C12Q2521/331

摘要： Novel methods are herein provided for high-throughput, dual analysis of DNA methylation and gene expression, and triple analysis of DNA methylation, gene expression and gene-associated histone acetylation in cancer cells using arrayed expressed CpG island sequence tags (ECISTs). ECISTs correspond to genomic DNA fragments comprising GC-rich segments along with promoter and/or exon (e.g., first exon) portions of genes. The GC-rich segments are useful for screening hypermethylated CpG sites in cancer cells, while the corresponding promoter and exon-containing portions are useful for determining corresponding transcript levels and assessing histone acetylation. Also provided are high-throughput methods for either confirming methylation-dependent gene silencing, or identifying therapeutically effective demethylating agents, using the ECIST array panels to identify hypermethylated loci, and measure expression levels thereof after cellular exposure to demethylating agents. Further provided are high-throughput methods for distinguishing between direct (primary) demethylation-dependent gene up-regulation, and indirect (secondary) demethylation-dependent up-regulation within apparent epigenetic cascades.