摘要:
The object of the invention is to provide an infrared circular dichroism measuring apparatus that improves the measuring time and the measuring accuracy. An infrared circular dichroism measuring apparatus 101 comprising: AC signal extractors 110-112 where an interference light beam from an IR light source 102 which has passed an interferometer 103 is converted into a clockwise and a counterclockwise circularly polarized light beams and is irradiated on a sample to extract from a detected signal of detector 107 an interferogram by each of the circularly polarized light beams; DC signal extractors 113, 112 for extracting an interferogram by the IR absorption of the sample; a calculator 114 for figuring out the circular dichroism; and a selective transmitter 120 for narrowing down the wavelength region to be measured based on an IR absorption wavelength region corresponding to a vibration mode of the structure to be measured in the sample molecule.
摘要:
A probe microscope (120) for causing a sample (112) and a tip portion (118a) of a probe (118) on the sample side to approach each other, detecting an interaction between the sample (112) and the sample-side probe tip portion (118a), and obtaining surface information of the sample (112) from the interaction, the probe (118) being a flexible needle-like probe; the probe microscope (120) comprises vibrating means (122) for rotating the probe (118) while flexing the tip portion (118a) thereof so as to draw a circle having a size corresponding to an increase and decrease in the interaction between the sample surface (112) and the tip portion (118a), and detecting means (124) for detecting the increase and decrease in the size of the circle drawn by the sample-side probe tip portion (118a) due to the interaction and obtaining information about the distance between the sample (112) and the tip portion (118a).
摘要:
A pressure control apparatus is used in an apparatus for effecting extraction, chromatographic separation, and fractionation. The pressure control apparatus is intended to reduce the amount of a fluid stagnating in a valve, and is arranged to cyclically open and close the valve, thereby controlling the level of pressure. An apparatus for effecting extraction, chromatographic separation, and fractionation is designed to perform in an on-line manner the extraction of soluble components contained in a sample as well as the separation and fractionation of the components. A chromatography is connected to a downstream side of a supercritical fluid extraction device, and the aforesaid pressure control apparatus is used as back-pressure regulators (9, 18) for the chromatography and the extraction device. An extraction fluid and an elution fluid which are used in a mobile phase may be a common fluid (Fig. 1) or separate fluids (Fig. 10). At the time of extraction, the soluble components of the sample within an extraction vessel (6) is extracted by a supercritical fluid, and is delivered to an adsorption column (14). The supercritical fluid is converted into a gas by reducing the pressure within the adsorption column and thus the solubility of the fluid is lowered so that the resultant extract is adsorbed by an adsorbent packed in this column. At the time of chromatographic separation, the pressure within the adsorption column (14) is maintained at a critical pressure or higher and thus an extract is eluted by the supercritical fluid and is delivered to a separation column (16), in which the extract is separated into individual components. Then, when the pressure within the adsorption column is reduced, the supercritical fluid is gasified and the result components are collected as fractions.
摘要:
The present invention relates to a split stream flow cell which is used for a UV detector of liquid chromatography and in which a UV light for detecting absorption of the light is transmitted through a sample solution flowing in the cell. It is an object of the present invention to reduce the influences of both the flow effect and the liquid lens effect without cell volume being made too large. To this end, a split stream flow cell of the present invention comprises a split flow channel (12) which passes through a cell block (10) and which has a central portion communicating with an inlet channel (18), both ends communicating with an outlet channel (38), and both sides symmetrical with respect to the central portion, the diameter thereof being increased from a given position in the central portion toward both ends.
摘要:
This invention provides a circular dichroism measurement device that is capable of measuring infrared circular dichroism with a suitable signal-to-noise ratio even if a sample has two or more absorbances. This circular dichroism measurement device comprises: a laser light source (QCL) capable of sweeping the wavenumber of laser light within an infrared wavenumber range including at least one absorption peak of a sample; a sample chamber (15) within which the sample is disposed; a polarization modulation element (14) that modulates the polarization state of swept laser light having a specific wavenumber before or after the laser light passes through the sample; a detector (17) that detects intensity variation in the laser light that has passed through the sample and had the polarization state thereof modulated; and a signal processing unit (40) that extracts a direct current component (DC) and an alternating current component (AC) synchronized with the modulation frequency from a detection signal from the detector (17) and calculates an infrared circular dichroism value for the sample on the basis of the ratio (AC/DC) of the components.
摘要:
A chromatography system has a multi-channel detection device 10 including a flow cell 12 to which a specimen is supplied, optics for directing light from light sources 11 to the flow cell 12 and outputting light that has passed through the flow cell, and a multi-channel detector 13 disposed on an output of the optics. The optics has a function of dispersing light in wavelength in an optical path. The detector 13 receives the light dispersed in wavelength. The multi-channel detection device 10 also has a signal processing part 14 connected to the detector. The chromatography system has a data processing apparatus 20. The signal processing circuit has a function of calculating an absorbance by absorbance = -log 10 ( I / I 0 ) using the intensity I of light having a wavelength to be measured that is outputted from the detector and a reference intensity I 0 of light that is an average of intensities of light having different wavelengths that is produced at the same point of time as the light having a wavelength to be measured.
摘要:
A confocal microscopy apparatus (10) for irradiating a measuring region of a specimen with light and for obtaining spectral information of light from the measuring region, comprising: a specimen irradiation means (20); an objective lens (32); an imaging lens (34); an aperture stop (36) having a plurality of openings disposed on an imaging plane of the imaging lens; and spectrum obtaining means (50, 60) for detecting and for obtaining the spectral information of light passing through each of the openings. Therefore, the spectral information according to light passing through each opening maintains the same spatial resolution as that obtained with the aperture stop having a circular opening, and the spectral information of light from the plurality of points of the specimen can be obtained altogether. Consequently, distribution measurement of a given measuring region of the specimen can be executed with high resolution and at high speed.