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149 条记录 (耗时 0.024 秒)

    IMPROVED METHOD FOR MORE EFFICIENT ELECTROPORATION
    22.
    发明公开
    IMPROVED METHOD FOR MORE EFFICIENT ELECTROPORATION 审中-公开
    改进方法高效电穿孔

    公开(公告)号：EP1121450A1

    公开(公告)日：2001-08-08

    申请号：EP99951796.4

    申请日：1999-10-06

    申请人： STRATAGENE

    发明人： GREENER, Alan, L. ,  JERPSETH, Bruce, D.

    IPC分类号： C12N15/64

    CPC分类号： C12N15/87 ,  C12N13/00 ,  C12N15/64

    摘要： This invention provides improved electroporation methods for transferring nucleic acids of interest into host cells, wherein the host cells are (1) suspended in a substantially non-ionic solution comprising at least one sugar or sugar derivative, (2) mixed with the nucleic acids of interest, and (3) electrically treated. Also, this invention provides for kits used in the method for transferring nucleic acids into host cells.

    COLLECTIONS OF UNIQUELY TAGGED MOLECULES
    23.
    发明公开
    COLLECTIONS OF UNIQUELY TAGGED MOLECULES 审中-公开
    具体标明分子集合

    公开(公告)号：EP1021563A2

    公开(公告)日：2000-07-26

    申请号：EP98953247.8

    申请日：1998-10-05

    申请人： STRATAGENE

    发明人： SORGE, Joseph, A.

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6816 ,  C07K1/047

    摘要： A method for functionally labeling large numbers of molecular species in a mixture of different species is provided. Each molecular species is labeled with a species-unique tag which allows for the rapid identification of each labeled species. The species-unique tag is identifiable by a uniquely identifiable property or characteristic.

    IMPROVED CIRCULAR SITE-DIRECTED MUTAGENESIS
    24.
    发明公开
    IMPROVED CIRCULAR SITE-DIRECTED MUTAGENESIS 失效
    改进的位点特异性诱变INCIRCULÄRERDNA

    公开(公告)号：EP0871778A1

    公开(公告)日：1998-10-21

    申请号：EP96942905.0

    申请日：1996-12-05

    申请人： STRATAGENE ,  CHILDREN'S MEDICAL CENTER CORPORATION

    发明人： BAUER, John, C. ,  WRIGHT, Dowain, A. ,  BRAMAN, Jeffrey, C. ,  GECHA, Raif, S.

    IPC分类号： C12N15 ,  C12Q1

    CPC分类号： C12N15/102 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6853 ,  C12Q2600/156 ,  Y10S435/81 ,  C12Q2525/307 ,  C12Q2521/319 ,  C12Q2525/185 ,  C12Q2521/331 ,  C12Q2521/101 ,  C12Q2531/125

    摘要： The invention provides improved methods of introducing site-directed mutations into circular DNA molecules of interest by means of mutagenic primer pairs. The mutagenic primer pairs are also selected so as to be either completely complementary or partially complementary to each other, wherein the mutation site (or sites) is located within the region of complementarity. A mutagenic primer pair is annealed to opposite strands of a circular DNA molecule containing the DNA sequence to be mutagenized. After annealing, first and second mutagenized DNA strands, each incorporating a member of the mutagenic oligonucleotide primer pair is synthesized by a linear cyclic amplification reaction. After the linear cyclic amplification mediated synthesis step is completed, the reaction mixture is treated with a selection enzyme that digests the parental template strands. After the digesting step, a double-stranded circular DNA intermediate is formed. The double-stranded circular DNA intermediates are transformed in suitable competent host cells and closed circular double-stranded DNA corresponding to the parental template molecules, but containing the desired mutation or mutations of interest, may be conveniently recovered from the transformed cells. The invention also provides kits for site-directed mutagenesis in accordance with methods of the present invention.

    High fidelity DNA polymerase compositions and uses therefor
    30.
    发明公开
    High fidelity DNA polymerase compositions and uses therefor 有权
    高保真DNA聚合酶-Zusammensetzungen和Verwendungendafür

    公开(公告)号：EP2110432A2

    公开(公告)日：2009-10-21

    申请号：EP09100097.6

    申请日：2002-12-17

    申请人： Stratagene California

    发明人： Hogrefe, Hooly ,  Borns, Michael ,  Sorge, Joseph A.

    IPC分类号： C12N9/12 ,  C12P19/34

    CPC分类号： C12N9/1252

    摘要： The subject invention relates to compositions comprising an enzyme mixture which comprises a first enzyme and a second enzyme; where the first enzyme comprises a DNA polymerization activity and the second enzyme comprises an 3'-5' exonuclease activity and a reduced DNA polymerization activity. The invention also relates to the above compositions in kit format and methods for high fidelity DNA synthesis using the subject compositions of the invention.

    摘要翻译： 本发明涉及包含含有第一酶和第二酶的酶混合物的组合物; 其中第一种酶包含DNA聚合活性，第二种酶包含3'-5'核酸外切酶活性和降低的DNA聚合活性。 本发明还涉及使用本发明的主题组合物的用于高保真DNA合成的试剂盒形式的上述组合物和方法。