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    Plasmid pHM 1519
    21.
    发明公开
    Plasmid pHM 1519 失效
    质粒pHM 1519

    公开(公告)号：EP0078537A3

    公开(公告)日：1984-04-18

    申请号：EP82110095

    申请日：1982-11-02

    申请人： AJINOMOTO CO., INC.

    发明人： Matsui, Hiroshi ,  Miwa, Kiyoshi ,  Terabe, Mahito ,  Nakamori, Shigeru ,  Sano, Konosuke

    IPC分类号： C12N15/00 ,  C12N01/20 ,  C12R01/15

    CPC分类号： C12N15/77

    摘要： An essentially pure plasmid is provided, which is characterized by a molecular weight of 1.8 megadalton and the restriction endonuclease-cleavage chart shown in the Figure, and which is capable of propagating in a Coryneform glutamic acid producing bacterium as a host strain. This plasmid may be inserted by a gene which is responsible for the production of a product and the hybrid plasmid may be incorporated in Coryneform glutamic acid producing bacteria as recipient organisms.

    摘要翻译： 提供基本上纯的质粒，其特征在于分子量为1.8兆道尔顿以及图中所示的限制性内切核酸酶切割图，并且其能够在棒杆菌谷氨酸生产菌中作为宿主菌株繁殖。 该质粒可以由负责生产产物的基因插入，并且可以将杂合质粒掺入作为受体生物体的棒状杆菌型谷氨酸生产菌中。

    Method for producing L-threonine by fermentation
    22.
    发明公开
    Method for producing L-threonine by fermentation 失效
    通过发酵生产L-噻吩的方法

    公开(公告)号：EP0066129A3

    公开(公告)日：1984-02-22

    申请号：EP82104088

    申请日：1982-05-11

    申请人： AJINOMOTO CO., INC.

    发明人： Tsuchida, Takayasu ,  Miwa, Kiyoshi ,  Nakamori, Shigeru

    IPC分类号： C12P13/08 ,  C12N15/00 ,  C12R01/13 ,  C12R01/15

    CPC分类号： C12N15/77 ,  C12N15/52 ,  C12P13/08

    摘要： New L-threonine-producing strains of the genus Brevibacterium and Corynebacterium are obtained by separating a plasmid DNA from a microorganism of the genus Brevibacterium and Corynebacterium, inserting into the plasmid DNA a fragment of chromosomal DNA derived from a DNA-donor strain of the genus Brevibacterium or Corynebacterium resistant to a-amino-p-hydroxy valeric acid to obtain a recombinant plasmid DNA, incorporating the recombinant plasmid DNA into a recipient strain of the genus Brevibacterium or Corynebacterium which is sensitive to α-amino-β-hydroxy valeric acid and isolating a strain transformed to become resistant to α-amino-β-hydroxy-valeric acid. By aerobically cultivating the new strains in an aqueous culture medium L-threonine may be obtained in high yields.

    Method for producing L-threonine by fermentation
    24.
    发明公开
    Method for producing L-threonine by fermentation 失效
    Verfahren zur Herstellung von L-Threonin durchGärung。

    公开(公告)号：EP0066129A2

    公开(公告)日：1982-12-08

    申请号：EP82104088.8

    申请日：1982-05-11

    申请人： AJINOMOTO CO., INC.

    发明人： Tsuchida, Takayasu ,  Miwa, Kiyoshi ,  Nakamori, Shigeru

    IPC分类号： C12P13/08 ,  C12N15/00

    CPC分类号： C12N15/77 ,  C12N15/52 ,  C12P13/08

    摘要： New L-threonine-producing strains of the genus Brevibacterium and Corynebacterium are obtained by separating a plasmid DNA from a microorganism of the genus Brevibacterium and Corynebacterium, inserting into the plasmid DNA a fragment of chromosomal DNA derived from a DNA-donor strain of the genus Brevibacterium or Corynebacterium resistant to a-amino-p-hydroxy valeric acid to obtain a recombinant plasmid DNA, incorporating the recombinant plasmid DNA into a recipient strain of the genus Brevibacterium or Corynebacterium which is sensitive to α-amino-β-hydroxy valeric acid and isolating a strain transformed to become resistant to α-amino-β-hydroxy-valeric acid.
    By aerobically cultivating the new strains in an aqueous culture medium L-threonine may be obtained in high yields.

    摘要翻译： 通过从短杆菌属和棒状杆菌属的微生物中分离出质粒DNA，从而获得了短杆菌属和棒状杆菌属的新的L-苏氨酸生产菌株，将来自该属的DNA供体菌株的染色体DNA片段插入质粒DNA 对α-氨基-β-羟基戊酸有抗性的短杆菌属或棒状杆菌以获得重组质粒DNA，将重组质粒DNA掺入对α-氨基-β-羟基戊酸敏感的短杆菌属或棒状杆菌属的受体菌株中， 分离转化成对α-氨基-β-羟基 - 戊酸具有抗性的菌株。 通过在水性培养基中有氧地培养新菌株，可以高产率获得L-苏氨酸。

    L-cysteine producing microorganism and a method for producing L-cysteine
    26.
    发明公开
    L-cysteine producing microorganism and a method for producing L-cysteine 有权
    L-Zystein-produzierender Mikroorganismus und Verfahren zur Produktion von L-Zystein

    公开(公告)号：EP1849874A1

    公开(公告)日：2007-10-31

    申请号：EP07003442.6

    申请日：2005-03-04

    申请人： Ajinomoto Co., Inc.

    发明人： Takagi, Hiroshi ,  Nakamori, Shigeru ,  Wada, Masaru ,  Mori, Hirotada Nara Institute of Science and Technology

    IPC分类号： C12P13/12 ,  C12N9/10 ,  C12N9/88 ,  C12N15/54 ,  C12N15/60

    CPC分类号： C12P13/12

    摘要： L-cysteine is produced by culturing an Escherichia bacterium having L-cysteine producing ability and containing a gene encoding an O-acetylserine sulphydrylase B or MalY regulatory protein that is modified so that cysteine desulfhydrase activity is reduced or eliminated. The bacterium is cultured in a medium to produce and cause accumulation of L-cysteine in the medium, and collecting L-cysteine from the medium.

    摘要翻译： 通过培养具有L-半胱氨酸生产能力的埃希氏菌属细菌并含有编码O-乙酰丝氨酸巯基酶B或MalY调节蛋白的基因来产生L-半胱氨酸，其被修饰以使得半胱氨酸脱硫酸酶活性降低或消除。 该细菌在培养基中培养以产生并导致培养基中L-半胱氨酸的积累，并从培养基中收集L-半胱氨酸。

    L-cysteine-producing microorganism and a method for producing L-cysteine
    27.
    发明公开
    L-cysteine-producing microorganism and a method for producing L-cysteine 有权
    L-半胱氨酸生产微生物和生产L-半胱氨酸的方法

    公开(公告)号：EP1571223A3

    公开(公告)日：2005-11-30

    申请号：EP05004829.7

    申请日：2005-03-04

    申请人： Ajinomoto Co., Inc.

    发明人： Takagi, Hiroshi ,  Nakamori, Shigeru ,  Wada, Masaru ,  Mori, Hirotada, c/o Nara Institute of

    IPC分类号： C12P13/12 ,  C12N9/10 ,  C12N9/88 ,  C12N15/54 ,  C12N15/60

    CPC分类号： C12P13/12

    摘要： L-cysteine is produced by culturing an Escherichia bacterium having L-cysteine producing ability and containing a gene encoding an O-acetylserine sulphydrylase B or MalY regulatory protein that is modified so that cysteine desulfhydrase activity is reduced or eliminated. The bacterium is cultured in a medium to produce and cause accumulation of L-cysteine in the medium, and collecting L-cysteine from the medium.

    摘要翻译： 通过培养具有L-半胱氨酸生产能力并含有编码O-乙酰丝氨酸硫化酶B或MalY调节蛋白的基因的埃希氏菌属细菌产生L-半胱氨酸，所述基因被修饰以使半胱氨酸脱硫酶活性降低或消除。 将细菌在培养基中培养以在培养基中产生并引起L-半胱氨酸蓄积，并从培养基中收集L-半胱氨酸。