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    (S)-alpha-PHENETHYLAMINE : PYRUVATE TRANSAMINASE
    21.
    发明公开
    (S)-alpha-PHENETHYLAMINE : PYRUVATE TRANSAMINASE 有权
    （S）-α-苯乙胺：吡咯烷磺酰胺酶

    公开(公告)号：EP1045025A1

    公开(公告)日：2000-10-18

    申请号：EP99951134.8

    申请日：1999-10-29

    申请人： KANEKA CORPORATION

    发明人： IWASAKI, Akira ,  YAMADA, Yukio ,  IKENAKA, Yasuhiro ,  YASOHARA, Yoshihiko ,  HASEGAWA, Junzo

    IPC分类号： C12N9/10 ,  C12P41/00

    CPC分类号： C12N9/1096 ,  C12P41/006

    摘要： Relating to an enzyme capable of efficiently converting a ketone compound to an optically active amino compound by transamination, and a process for preparing an optically active amino compound using the enzyme. An (S)-α-phenethylamine : pyruvate transaminase, which acts on (S)-α-phenethylamine and a ketone compound, thereby catalyzing transamination for forming acetophenone and an amino compound corresponding to the ketone compound; a process for preparing an optically active amino compound using the transaminase; and a method for culturing a microorganism for producing the above transaminase, comprising adding to a medium one or more compounds selected from the group consisting of propylamine, 1-butylamine, 2-butylamine, 2-pentylamine, isopropylamine and isobutylamine as an inducer for the enzyme when a microorganism for producing (S)-α-phenethylamine : pyruvate transaminase is cultured.

    摘要翻译： 关于能够通过转氨酶有效地将酮化合物转化为光学活性氨基化合物的酶，以及使用该酶制备光学活性氨基化合物的方法。 （S）-α-苯乙胺：丙酮酸转氨酶，其作用于（S）-α-苯乙胺和酮化合物，从而催化转氨酶形成苯乙酮和对应于酮化合物的氨基化合物; 使用转氨酶制备光学活性氨基化合物的方法; 以及培养用于制备上述转氨酶的微生物的方法，包括向培养基中加入一种或多种选自丙胺，1-丁胺，2-丁胺，2-戊胺，异丙胺和异丁胺的化合物作为诱导剂， 酶用于生产（S）-α-苯乙胺：丙酮酸转氨酶的微生物。

    MODIFIED ENZYME AND USE THEREOF
    23.
    发明公开
    MODIFIED ENZYME AND USE THEREOF 审中-公开

    公开(公告)号：EP3536782A1

    公开(公告)日：2019-09-11

    申请号：EP17866696.2

    申请日：2017-11-01

    申请人： Kaneka Corporation

    发明人： MATSUI, Misato ,  ITO, Noriyuki ,  YASOHARA, Yoshihiko

    IPC分类号： C12N15/09 ,  C12N9/02 ,  C12P21/02

    摘要： The present invention provides, by modifying a wild-type glutathione synthetase, a modified glutathione synthetase which has a more stably retained activity, particularly a higher thermal stability, and/or a transformant capable of producing the modified glutathione synthetase.
    A polypeptide in accordance with the present invention is a polypeptide which (a) is capable of carrying out a reaction of binding glycine to γ-glutamyl dipeptide and (b) has a higher thermal stability and/or a higher storage stability as compared with glutathione synthetase consisting of an amino acid sequence of SEQ ID NO: 1 shown in the Sequence Listing.

    NOVEL AMINO ACID DEHYDROGENASE, AND PROCESS FOR PRODUCING L-AMINO ACID, 2-OXO ACID OR D-AMINO ACID
    27.
    发明公开
    NOVEL AMINO ACID DEHYDROGENASE, AND PROCESS FOR PRODUCING L-AMINO ACID, 2-OXO ACID OR D-AMINO ACID 有权
    新的氨基酸脱氢酶AND METHOD FOR PRODUCING L-氨基酸，2-氧代氨基或D

    公开(公告)号：EP2374882A1

    公开(公告)日：2011-10-12

    申请号：EP09831685.4

    申请日：2009-12-08

    申请人： Kaneka Corporation

    发明人： KANAMARU, Hiroyuki ,  MIKI, Ryuuji ,  UEDA, Makoto ,  NANBA, Hironori ,  YASOHARA, Yoshihiko

    IPC分类号： C12N15/09 ,  C12N1/20 ,  C12N1/21 ,  C12N9/06 ,  C12P13/04

    CPC分类号： C12N9/0016 ,  C12N9/0012 ,  C12N15/70 ,  C12N15/74 ,  C12P7/40 ,  C12P13/04 ,  C12P13/22 ,  C12P41/006

    摘要： The present invention relates to a novel amino acid dehydrogenase, DNA encoding the enzyme, and a transformant having the DNA introduced therein. The present invention also relates to a process for producing an L-amino acid, 2-oxo acid or D-amino acid, which includes allowing the amino acid dehydrogenase or a microorganism or transformant capable of producing the enzyme to act on a substrate compound. The amino acid dehydrogenase has good reactivity even with an amino acid or a 2-oxo acid each having a bulky side chain such as an aromatic-ring-containing group, which acids are poorly reactive with conventional amino acid dehydrogenases. The amino acid dehydrogenase enables the inexpensive and highly efficient production of a useful optically active amino acid.

    摘要翻译： 本发明涉及一种新的氨基酸脱氢酶，编码DNA酶，并且具有引入其中的DNA的转化体。 因此，本发明涉及一种用于在L-氨基酸，2-氧代酸或D-氨基酸，其中包括使氨基酸脱氢酶或微生物或转化体能够产生作用于底物化合物的酶的产生。 的氨基酸脱氢酶具有良好的反应性，甚至在氨基酸或2-含氧酸各自具有大体积侧链：如上含芳香环的基团，其酸是与常规氨基酸脱氢酶不良反应性的。 氨基酸脱氢酶启用廉价且高效率的生产一种有用的光学活性的氨基酸的。

    METHOD FOR PRODUCTION OF OPTICALLY ACTIVE AMINE COMPOUND, RECOMBINANT VECTOR, AND TRANSFORMANT CARRYING THE VECTOR
    28.
    发明公开
    METHOD FOR PRODUCTION OF OPTICALLY ACTIVE AMINE COMPOUND, RECOMBINANT VECTOR, AND TRANSFORMANT CARRYING THE VECTOR 有权
    生产光学活性胺化合物的方法，重组载体以及携带载体的转化子

    公开(公告)号：EP2022852A1

    公开(公告)日：2009-02-11

    申请号：EP07744240.8

    申请日：2007-05-28

    申请人： Kaneka Corporation

    发明人： KAWANO, Shigeru ,  ITO, Noriyuki ,  YASOHARA, Yoshihiko

    IPC分类号： C12N15/00 ,  C12N1/21 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N15/09 ,  C12P13/00

    CPC分类号： C12P13/008 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/1096 ,  C12P13/001 ,  C12P13/002 ,  C12P17/10

    摘要： The present invention relates to a method for producing an optically-active amine compound. The method is characterized by using a transaminase (A), an α-keto acid reductase (B), and an enzyme (C), each having specific properties, in an identical reaction system to convert a ketone compound into a corresponding optically-active amine compound in which a carbon atom with an amino group bonded thereto serves as an asymmetric point. The present invention also relates to a recombinant vector for use in the method. The present invention makes it possible to efficiently produce an optically-active amine compound.

    摘要翻译： 本发明涉及光学活性胺化合物的制造方法。 该方法的特征在于在相同的反应体系中使用转氨酶（A），α-酮酸还原酶（B）和酶（C）各自具有特定性质以将酮化合物转化为相应的光学活性 其中具有与其键合的氨基的碳原子用作不对称点的胺化合物。 本发明还涉及用于该方法中的重组载体。 本发明使得可以有效地生产光学活性胺化合物。

    METHOD OF MODIFYING ENZYME AND OXIDOREDUCTASE VARIANT
    30.
    发明公开
    METHOD OF MODIFYING ENZYME AND OXIDOREDUCTASE VARIANT 审中-公开
    改变酶和氧化还原酶变异体的方法

    公开(公告)号：EP1416050A1

    公开(公告)日：2004-05-06

    申请号：EP02738904.8

    申请日：2002-07-02

    申请人： KANEKA CORPORATION

    发明人： NAKAI, Takahisa ,  MORIKAWA, Souichi ,  KIZAKI, Noriyuki ,  YASOHARA, Yoshihiko

    IPC分类号： C12N15/53 ,  C12N9/06 ,  C12N1/21 ,  C12P7/62

    CPC分类号： C12N9/0006 ,  C12P7/62

    摘要： An enzyme modifying method for converting the coenzyme-dependency of an oxidoreductase is developed. Using this method, a novel carbonyl reductase mutant capable of utilizing NADH as a coenzyme is provided. It is also intended to provide a process for enzymatically producing an optically active (S)-4-halo-3-hydroxybutyric ester by utilizing the carbonyl reductase mutant.
    A method for modifying an enzyme itself so as to convert the coenzyme-dependency of a carbonyl reductase which asymmetrically reduces a carbonyl compound to produce an optically active alcohol, a carbonyl reductase having such coenzyme dependency as has been converted from NADPH to NADH which is obtained by the above method, a DNA encoding this enzyme mutant, a plasmid carrying this DNA, a transformant obtained by the transformation with this plasmid, and a process for producing an optically active alcohol by using this enzyme mutant and/or this transformant.

    摘要翻译： 开发了用于转化氧化还原酶的辅酶依赖性的酶修饰方法。 使用该方法，提供了能够利用NADH作为辅酶的新型羰基还原酶突变体。 还旨在提供通过利用羰基还原酶突变体酶促生产光学活性（S）-4-卤代-3-羟基丁酸酯的方法。 一种修饰酶本身以转化羰基还原酶的辅酶依赖性的方法，该羰基还原酶不对称地还原羰基化合物以产生光学活性醇，具有从NADPH转化为NADH的这种辅酶依赖性的羰基还原酶，其获得 通过上述方法，通过使用该酶突变体和/或该转化体，编码该酶突变体的DNA，携带该DNA的质粒，通过该质粒转化而获得的转化体以及用于生产光学活性醇的方法。