公开(公告)号：EP2057263A1

公开(公告)日：2009-05-13

申请号：EP07786912.1

申请日：2007-06-28

Applicant: BioGasol IPR ApS

Inventor： MIKKELSEN, Marie Just ,  AHRING, Birgitte Kiær

IPC: C12M1/00 ,  C12N5/00

CPC classification number: C12P7/10 ,  C12M21/12 ,  C12M25/16 ,  C12M25/20 ,  C12M41/18 ,  C12P7/04 ,  C12P7/16 ,  C12P7/18 ,  C12P7/28 ,  C12P7/40 ,  C12P7/46 ,  C12P7/52 ,  C12P7/54 ,  C12P7/56 ,  Y02E50/10 ,  Y02E50/16 ,  Y02E50/17

Abstract: Production of fermentation products, such as ethanol and lactic acid in biofilm reactors by microorganisms immobilised on sterilised granular sludge.

公开(公告)号：EP1298198B1

公开(公告)日：2006-10-11

申请号：EP02022306.1

申请日：1999-03-17

Applicant: GYROS PATENT AB

Inventor： Thomas, Nicholas

IPC: C12M1/20 ,  C12M1/34 ,  B01L3/00

CPC classification number: B01L3/5027 ,  B01L3/502707 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/10 ,  B01L2300/0806 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0877 ,  B01L2300/10 ,  B01L2400/0409 ,  B01L2400/0688 ,  C12M23/16 ,  C12M23/38 ,  C12M25/16

公开(公告)号：EP1660629A2

公开(公告)日：2006-05-31

申请号：EP04778630.6

申请日：2004-07-19

Applicant: GLOBAL CELL SOLUTIONS, LLC.

Inventor： FELDER, Robin, A. ,  GILDEA, John, J.

IPC: C12N1/00

CPC classification number: C12N5/0075 ,  C12M23/14 ,  C12M23/20 ,  C12M25/14 ,  C12M25/16 ,  C12M41/46 ,  C12N2533/40 ,  C12N2533/54 ,  C12N2533/74 ,  C12Q1/02

Abstract: The present invention relates generally to the field of cell culture, which is a laboratory process used primarily for the growth, propagation, and production of cells for analysis and the production and harvesting of cell products. The present invention comprises functionalized and/or engineered hydrogel microcarriers that exhibit any or all of the following properties: controllable buoyancy, ferro- or paramagnetism, molecular or fabricated reporting elements, and optical clarity. The microcarriers are used in a bioreactor that employs external forces to control said microcarrier kinetic energy and translational or positional orientation in order to facilitate cell growth and/or cellular analysis. The bioreactor can be part of an automated system that employs any or all of the following; a microcarrrier manufacturing method, a monitoring method, a cell culture method, and an analytical method. Either a single bioreactor or a plurality of bioreactors are used in the automated system to enable cell culture and analysis with a minimum of human intervention.

公开(公告)号：EP1383909A4

公开(公告)日：2006-05-24

申请号：EP02713883

申请日：2002-03-21

Applicant: BRIGHAM & WOMENS HOSPITAL

Inventor： PAOLETTI LAWRENCE C ,  MALIN GENNADY

IPC: C12M1/34 ,  C12M3/02 ,  C12Q1/00 ,  C12M1/00 ,  C12M3/00 ,  C12N1/00 ,  C12Q1/02

CPC classification number: C12M35/08 ,  C12M23/08 ,  C12M25/02 ,  C12M25/06 ,  C12M25/16

Abstract: The present invention is directed to an apparatus that can be used for co-culturing bacteria and eukaryotic cells. The apparatus allows the bacteria to be grown under steady state conditions and then perfused over the eukaryotic cells. The invention also includes a variety of methods for studying the attachment and invasion of host eukaryotic cells by bacteria.

公开(公告)号：EP1068348B1

公开(公告)日：2006-05-24

申请号：EP99917898.1

申请日：1999-03-27

Applicant: Roche Diagnostics GmbH ,  Institute of Protein Research

Inventor： BIRYUKOV, Sergey Vladimirovich ,  SIMONENKO, Peter Nikolayevich ,  SHIROKOV, Vladimir Anatolievich ,  MAJOROV, Sergey Gennadievich ,  SPIRIN, Alexander Sergeyevich

IPC: C12P21/02 ,  C12M1/40

CPC classification number: C12M23/34 ,  C12M23/02 ,  C12M25/16 ,  C12M29/04 ,  C12M29/16 ,  C12M29/18 ,  C12P21/02

Abstract: The present invention provides a method for synthesis polypeptides in a cell-free system by which products of synthesis are branched in a low molecular weight fraction and a fraction which contains high molecular weight components with the target polypeptide, the main part of the low molecular weight fraction is removed via at least one part of the second porous barrier, the ratio of the volume of the fractions of feed solution and expendable components to the volume of the fraction containing the target polypeptide is chosen, modes of supply of the feed solution and expendable components of the fraction are realized.

公开(公告)号：EP1211309A4

公开(公告)日：2003-02-12

申请号：EP00953532

申请日：2000-08-21

IPC: C12M1/40 ,  C12M3/00 ,  C12M3/02 ,  C12N7/00

CPC classification number: C12N7/00 ,  C12M25/16 ,  C12M41/00 ,  C12N2770/24251

Abstract: A method for proliferating a hepatitis virus such as HCV and an apparatus therefor. A method of proliferating cells (for example, hepatocytes), which are less adhesive to a carrier, in a large amount over a long time. More particularly, the above method comprises infecting hepatocytes, which are maintained in a radial flow type hepatocyte bioreactor consisting of a main bioreactor unit containing the hepatocytes carried on a particulate porous carrier and a liquid culture medium flown from the periphery of the main bioreactor unit toward the center thereof, with a hepatitis virus; continuously flowing the liquid culture medium from the periphery of the main bioreactor unit toward the center thereof; and thus proliferating the hepatitis virus in the hepatocytes thus infected.

公开(公告)号：EP1174497A1

公开(公告)日：2002-01-23

申请号：EP00115568.8

申请日：2000-07-19

Applicant: Technodop Ltd. (Société de Droit Irlandais)

Inventor： Vetillard, Jérôme ,  Herodin, Francis ,  Caterini, Richard

IPC: C12M3/06

CPC classification number: C12M35/08 ,  C12M25/16 ,  C12M29/04

Abstract: L'invention concerne une chambre de culture cellulaire à au moins deux membranes planes filtrantes à seuil de coupure différent, délimitée par une enveloppe à axe de symétrie qui est formée d'une paroi latérale externe, de deux parois d'extrémités et d'entrées et de sorties de milieux liquides dynamiques, ainsi qu'un bioréacteur contenant ladite chambre de culture pour la culture extracorporelle de cellules animales.

Abstract translation: 本发明涉及具有至少两个具有不同切断面的平面过滤膜的细胞培养室，其由具有由外侧壁，两个端壁和用于动态液体介质的入口端口和出口形成的对称轴线的包络限定，以及 含有用于动物细胞体外培养的培养室的生物反应器。

公开(公告)号：EP0817965A1

公开(公告)日：1998-01-14

申请号：EP96908028.0

申请日：1996-03-29

Applicant: NOVO NORDISK A/S

Inventor： THASTRUP, Ole ,  SCUDDER, Kurt ,  RUZICKA, Jaromir

IPC: G01N33 ,  C12M1 ,  C12Q1

CPC classification number: G01N33/502 ,  C12M25/16 ,  C12M41/46 ,  G01N33/5008

Abstract: A rapid automated method for identifying biologically active substances by their effect on living cells comprises the steps: a) culturing cells on/within a matrix that fluidically can be controlled; b) loading the cells with luminescent chemical molecules or having them previously transfected with recombinant molecules having luminescent properties; c) injecting a well defined volume of the cell carrying matrix suspension into a flow fluid which transports the suspension into a flow chamber having a wall through which the content of said flow chamber may be monitored; d) perifusing the cells with a sample containing a potentially biologically active substance; e) measuring the luminescence response of the content in the flow chamber; f) flushing the flow chamber to make it ready for a new testing cycle.

公开(公告)号：EP0670890B1

公开(公告)日：1997-05-28

申请号：EP94901854.3

申请日：1993-11-23

Applicant: Cultor Ltd.

Inventor： LOMMI, Heikki ,  SWINKELS, Wilhelmus, Jacobus, Petrus, Maria ,  VILJAVA, Timo, Tapio ,  HAMMOND, Roger, Charles

IPC: C12M1/40

CPC classification number: C12N5/0068 ,  C12M25/16 ,  C12N2533/78

Abstract: The invention relates to a bioreactor with immobilized lactic acid bacteria which is characterized in that the bacteria are fixed on the surface of a substantially non-compressible carrier which is composed of a continuous, porous matrix or of dimpled or reticular porous particles, the matrix or the particles having a structure of a loosely associated plurality of microparticles or microfibers which are bound together chemically, adhesively or mechanically at least at some contact points between the individual microparticles or microfibers. Preferably the microparticles or microfibers comprise or consist of a material, preferably a resin, having anion exchanger capability. A preferred embodiment of the carrier includes DEAE-cellulose, the microparticles and microfibers being agglomerated with polystyrene. The above bioreactor can be applied in the large-scale production of products resulting from the metabolism of lactic acid bacteria and in the modification of the composition of the feedstream.

公开(公告)号：EP0580761A4

公开(公告)日：1995-11-22

申请号：EP92911278

申请日：1992-04-09

Applicant: PERSEPTIVE BIOSYSTEMS INC

Inventor： AFEYAN NOUBAR B

IPC: C12M1/40 ,  C12N11/00 ,  C12N11/08 ,  C12P1/00

CPC classification number: C12N11/00 ,  C12M21/18 ,  C12M25/16 ,  C12N11/08

Abstract: Perfusive matrices (10) useful in immobilized enzyme reactors (IMERs) are disclosed. The matrices define first and second interconnected sets of pores and an intraparticle high surface area (18) capable of immobilizing enzymes, in fluid communication with the second set of pores. The first and second sets of pores are embodied, for example, as the interstices (12) among particles and throughpores (14) within the particles. The particles also contain diffusive transport pores (16). The dimensions of the first and second sets of pores are such that, at fluid flow rates and pressure drops practical for immobilized enzyme reactions, convective flow occurs in both pore sets, and the convective flow rate exceeds the rate of diffusion of the substrate and product to and from immobilized enzymes within the particles. This approach allows rapid enzyme loading and substrate conversion reactions at optimal catalytic flow rates.