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    NOVEL VECTOR
    21.
    发明公开
    NOVEL VECTOR 有权
    NEUARTIGER VEKTOR

    公开(公告)号：EP1630233A1

    公开(公告)日：2006-03-01

    申请号：EP04745724.7

    申请日：2004-06-03

    申请人： NIPPON PAPER INDUSTRIES CO., LTD.

    发明人： NANTO, Kazuya c/o Nippon Paper Industries Co., Ltd ,  WATANABE, Keiko c/o Nippon Paper Industries Co., L ,  EBINUMA, Hiroyasu c/o Nippon Paper Industries Co.,

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8213 ,  C12N15/8209 ,  C12N15/821

    摘要： In order to provide a vector and a method for introducing one copy of a desired gene to a predetermined position of a host DNA at a high frequency without any bad influences upon the host cell and a vector and a method for deleting or inverting a host DNA at a predetermined position without a crossing step and without any bad influences upon the host cell. A vector which has an introduction cassette inserted between two site-specific recombinase recognition sequences and a site-specific recombinase gene which recognizes these site-specific recombinase recognition sequences, wherein the recombinase gene is positioned outside the introduction cassette, is used, and this is introduced into a host cell having a DNA in which one or two site-specific recombinase recognition sequences are present. A recombinant of interest can be efficiently obtained by positioning a lethal induction gene or a morphological abnormality induction gene outside the introduction cassette, together with the site-specific recombinase gene.

    摘要翻译： 为了提供一种将所需基因的一个拷贝以高频率引入宿主DNA的预定位置而不对宿主细胞没有任何不良影响的载体和方法，以及用于删除或翻译宿主DNA的方法 在没有交叉步骤的预定位置，对宿主细胞没有任何不良影响。 使用具有插入在两个位点特异性重组酶识别序列之间的引入盒和识别这些位点特异性重组酶识别序列的位点特异性重组酶基因的载体，其中重组酶基因位于引入盒外侧，这是 引入具有其中存在一个或两个位点特异性重组酶识别序列的DNA的宿主细胞。 通过将导入盒外的致死诱导基因或形态异常诱导基因与位点特异性重组酶基因一起定位，可以有效地获得感兴趣的重组体。

    METHOD OF ENCODING INFORMATION IN NUCLEIC ACIDS OF A GENETICALLY ENGINEERED ORGANISM
    22.
    发明公开
    METHOD OF ENCODING INFORMATION IN NUCLEIC ACIDS OF A GENETICALLY ENGINEERED ORGANISM 有权
    用于信息的加密程序在DNA在基因改造生物

    公开(公告)号：EP1373531A1

    公开(公告)日：2004-01-02

    申请号：EP01986901.5

    申请日：2001-12-19

    申请人： Icon Genetics AG

    发明人： GLEBA, Yuri ,  KLIMYUK, Victor

    IPC分类号： C12N15/82 ,  C12Q1/68

    CPC分类号： C12N15/8265 ,  C12N15/8209 ,  C12N15/821 ,  C12Q1/6813 ,  C12Q2563/185

    摘要： A method of producing a genetically engineered organism, by (a) incorporating into said organism a functional DNA sequence and (b) incorporating into said organism a non-functional DNA sequence, wherein said non-functional DNA sequence corresponds to the results of the application of a predefined coding scheme to an information message, said information message is related to said functional DNA sequence, said predefined coding scheme provides a mapping from a plurality of possible information messages into a plurality of DNA sequences. Said functional and said non-functional DNA sequence remain linked during reproduction of the organism for a practically relevant period of time.

    USE OF A SELECTABLE MARKER GENE IN SUGAR BEET PROTOPLASTS TRANSFORMATION METHOD AND SYSTEM
    28.
    发明公开
    USE OF A SELECTABLE MARKER GENE IN SUGAR BEET PROTOPLASTS TRANSFORMATION METHOD AND SYSTEM 审中-公开
    VERWENDUNG EINES SELEKTIERBAREN MARKERGENS IN TRANSFORMATIONSVERFAHRENFÜRZUCKERRÜBENPROTOPLASTENUND SYSTEM

    公开(公告)号：EP3155000A1

    公开(公告)日：2017-04-19

    申请号：EP15730106.0

    申请日：2015-06-12

    申请人： SESVanderHave N.V.

    发明人： WEYENS, Guy ,  LEFÈBVRE, Marc ,  THAMMANNAGOWDA, Shivegowda ,  LOMMEL, Murielle

    IPC分类号： C07K14/415 ,  C12N15/82

    CPC分类号： C12N15/821 ,  A01H1/02 ,  A01H1/04 ,  C12N9/88 ,  C12N15/52 ,  C12N15/8209 ,  C12N15/8241 ,  C12N15/8279 ,  C12N15/8281 ,  C12N15/8282 ,  C12N15/8285 ,  C12N15/8286

    摘要： A method for the transformation of sugar beet protoplasts includes obtaining protoplasts from stomatal guard cells isolated from a sugar beet plant. The protoplasts are transformed with a nucleic acid construct including a nucleotide sequence of interest and a selection marker sequence. One or more ALS inhibitors at a concentration that is lethal to the in vitro culture of the protoplasts are applied to an in vitro culture of the protoplasts. Sugar beet plants are regenerated from the surviving protoplasts having integrated the nucleic acid construct including the sequence of interest and the selection marker sequence. The selection marker sequence is the mutated BvALS113 sequence carrying in its sequence a mutation at amino acid 113 position from Alanine to Tyrosine.

    摘要翻译： 本发明涉及一种用于转化甜菜原生质体的方法，包括以下步骤： - 从分离自甜菜植物的气孔保护细胞获得原生质体， - 用包含感兴趣的核苷酸序列的核酸构建体转化原生质体，以及 选择标记序列， - 应用于所述原生质体的体外培养，一种或多种浓度对原生质体的体外培养致死的ALS抑制剂，以及从具有整合核酸的存活的原生质体再生甜菜植物 构建体，其包含感兴趣的序列和选择标记序列，其中选择标记序列是突变的BvALS113序列，其在氨基酸113位置从丙氨酸到酪氨酸的突变。

    METHODS AND VECTORS FOR PRODUCING TRANSGENIC PLANTS
    30.
    发明公开
    METHODS AND VECTORS FOR PRODUCING TRANSGENIC PLANTS 审中-公开
    生产转基因植物的方法和载体

    公开(公告)号：EP3023499A1

    公开(公告)日：2016-05-25

    申请号：EP15197617.2

    申请日：2009-07-16

    申请人： Monsanto Technology LLC

    发明人： GILBERTSON, Larry ,  JOHNSON, Susan Jane

    IPC分类号： C12N15/82 ,  A01H5/00

    CPC分类号： C12N15/8216 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/821 ,  C12N15/8218 ,  C12N15/8241

    摘要： Methods of, and compositions for, assembling one or more transcription units in a genome without a linked selectable marker or other unwanted transcription unit are provided. Also provided methods of, and compositions for, assembling one or more transcription units in a genome with a reduced frequency of vector backbone.

    摘要翻译： 提供了用于在基因组中组装一个或多个转录单元而没有连接的选择标记或其他不想要的转录单元的方法和组合物。 还提供了组装基因组中一个或多个转录单元的方法和组合物，其载体骨架频率降低。