公开(公告)号：EP3242934A1

公开(公告)日：2017-11-15

申请号：EP16735360.6

申请日：2016-01-06

申请人： The Board of Trustees of The Leland Stanford Junior University

发明人： CHAN, Charles K. F. ,  WEISSMAN, Irving L. ,  LONGAKER, Michael T.

IPC分类号： C12N5/0775 ,  A61K35/28

CPC分类号： A61K35/28 ,  A61K35/32 ,  A61K38/1709 ,  A61K38/1875 ,  A61L27/3834 ,  A61L27/54 ,  A61L2300/252 ,  A61L2430/02 ,  A61L2430/06 ,  A61P19/02 ,  A61P19/08 ,  C12N5/0668 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/415

摘要： Methods, compositions and kits for producing functional chondrocytes, skeletal cells, bone marrow stromal cells, and progenitor cells thereof are provided. These methods, compositions and kits find use in producing chondrocytes, osteoblasts, stromal cells, and progenitor cells thereof in vivo, or in vitro for transplantation, for experimental evaluation, as a source of lineage- and cell-specific products, and the like, for example for use in treating human disorders of the cartilage, bone and hematopoietic system. In some embodiments, specific combinations of protein factors are identified for reprogramming non-skeletal cells into bones, hematopoietic stroma, and chondrocytes, which may be provided in vitro or in vivo.

公开(公告)号：EP3234109A1

公开(公告)日：2017-10-25

申请号：EP15870718.2

申请日：2015-12-09

申请人： Janssen Biotech, Inc.

发明人： FRYER, Benjamin ,  LANIAUSKAS, Daina

IPC分类号： C12N5/071 ,  C12N5/02

CPC分类号： C12N5/0676 ,  C12N2500/02 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/60 ,  C12N2501/117 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/19 ,  C12N2501/385 ,  C12N2501/395 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/91 ,  C12N2501/999 ,  C12N2506/02

摘要： The present invention provides methods of differentiating pluripotent cells into beta cell using suspension clustering. The methods of the invention use control of one or more of pH, cell concentration, and retinoid concentration to generate a nearly homogenous population of PDX1/NKX6.1 co-expressing cells by suppressing precocious NGN3 expression and promoting NKX6.1 expression. Also, the nearly homogenous population of PDX1/NKX6.1 co-expressing cells may be further differentiated in vitro to form a population of pancreatic endocrine cells that co-express PDX1, NKX6.1, insulin and MAFA.

摘要翻译： 本发明提供了使用悬浮聚类将多能细胞分化成β细胞的方法。 本发明的方法使用pH，细胞浓度和类视黄醇浓度中的一种或多种的控制，通过抑制早熟NGN3表达和促进NKX6.1表达来产生PDX1 / NKX6.1共表达细胞的几乎均匀的群体。 而且，PDX1 / NKX6.1共表达细胞的几乎均匀的群体可以在体外进一步分化以形成共表达PDX1，NKX6.1，胰岛素和MAFA的胰腺内分泌细胞群。

公开(公告)号：EP3224350A1

公开(公告)日：2017-10-04

申请号：EP15863890.8

申请日：2015-11-24

申请人： Children's Medical Center Corporation

发明人： SANKARAN, Vijay G.

IPC分类号： C12N5/0789

CPC分类号： C12N5/0641 ,  A61K35/18 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/14 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/2303 ,  C12N2501/2306 ,  C12N2501/26 ,  C12N2501/415 ,  C12N2501/998 ,  C12N2506/02 ,  C12N2506/03

摘要： Disclosed herein are methods for producing red blood cells (RBCs) from a population of stem cells and/or progenitor cells. In at least one of the stem cells or progenitor cells, SH2B3 protein activity is decreased, SH2B3 mRNA level is decreased, and/or SH2B3 protein level is decreased. The methods provided herein permit the production of RBCs with increased quantity and/or quality as compared to a method using the same population of stem cells and/or progenitor cells without SH2B3 inhibition or disruption. Also provided herein are methods of use of the RBCs produced using the methods described herein.

摘要翻译： 本文公开了用于从干细胞和/或祖细胞群体产生红血细胞（RBC）的方法。 在至少一种干细胞或祖细胞中，SH2B3蛋白质活性降低，SH2B3 mRNA水平降低，和/或SH2B3蛋白质水平降低。 与使用不含SH2B3抑制或破坏的相同干细胞和/或祖细胞群的方法相比，本文提供的方法允许产生具有增加量和/或质量的RBC。 本文还提供使用本文所述方法产生的RBC的使用方法。

公开(公告)号：EP3204490A1

公开(公告)日：2017-08-16

申请号：EP15775703.0

申请日：2015-10-07

申请人： Max-Delbrück-Centrum für Molekulare Medizin in der Helmholtz-Gemeinschaft ,  University Of Bath

发明人： WANG, Jichang ,  IZSVÁK, Zsuzsanna ,  HURST, Laurence D.

IPC分类号： C12N5/0735 ,  C12N5/074

CPC分类号： C12N5/0606 ,  C12N5/0696 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/065 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/2306 ,  C12N2501/2311 ,  C12N2501/235 ,  C12N2501/33 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2501/42 ,  C12N2501/727 ,  C12N2510/00

摘要： One or more type 7 long terminal repeat (LTR7) nucleic acid sequences of type H human endogenous retroviruses (HERVH) (“LTR7/HERVH nucleic acid sequences”) can be used for identifying primate naïve pluripotent stem cells. LTR7/HERVH-associated transcription can be used as a marker for primate naive pluripotent stem cells. A reporter construct that includes LTR7/HERVH nucleic acid sequences can be used for optimizing culture conditions for naïve primate pluripotent stem cells. A cell growth medium can be used for cultivation of primate naive pluripotent stem cells, which can exhibit elevated levels of LTR7/HERVH-associated transcription in comparison to control cells.

摘要翻译： 本发明涉及H型人内源性逆转录病毒（HERVH）的一种或多种7型长末端重复序列（LTR7）核酸序列（“LTR7 / HERVH核酸序列”）用于鉴定灵长类幼稚多能干细胞的用途。 本发明涉及LTR7 / HERVH核酸序列作为标志物的用途，其中LTR7 / HERVH相关转录用作灵长类动物幼稚多能干细胞的标志物。 本发明还涉及除了使用所述报道分子之外还包含LTR7 / HERVH核酸序列的报道构建体，特别是用于优化幼稚灵长类多能干细胞的培养条件。 本发明还涉及用于培养灵长类幼稚多能干细胞的细胞生长培养基，与对照细胞相比，其优选表现出升高水平的LTR7 / HERVH-相关转录。

公开(公告)号：EP3192533A1

公开(公告)日：2017-07-19

申请号：EP15839965.9

申请日：2015-09-07

申请人： ORGAN TECHNOLOGIES, INC.

发明人： TSUJI, Takashi ,  TOYOSHIMA, Koh-ei

IPC分类号： A61L27/00 ,  C12N5/071

CPC分类号： A61L27/00 ,  A01K67/0271 ,  A01K2207/10 ,  A01K2207/12 ,  A01K2227/105 ,  A01K2267/025 ,  A01K2267/0331 ,  A61L27/36 ,  A61L27/3604 ,  A61L27/38 ,  A61L27/40 ,  A61L27/60 ,  C12N5/0696 ,  C12N2501/415 ,  C12N2502/1323

摘要： The present invention provides a method for efficiently manufacturing full-thickness skin with skin appendage. The present invention provides a method for manufacturing full-thickness skin with skin appendage, characterized in that said method comprises the following steps (a) - (d): (a) a step of stimulating an embryoid body with a bioactive substance that may activate the Wnt pathway, (b) a step of preparing a conjugate comprising all or a part of said embryoid body stimulated in step (a) and a scaffolding material, (c) a step of transplanting said conjugate prepared in said step (b) to an animal, and (d) a step of manufacturing full-thickness skin derived from said conjugate in said animal.

摘要翻译： 本发明提供一种有效制造具有皮肤附属物的全层皮肤的方法。 本发明提供了一种制造具有皮肤附属物的全层皮肤的方法，其特征在于，所述方法包括以下步骤（a） - （d）：（a）用可能活化的生物活性物质刺激胚状体的步骤 （b）制备包含在步骤（a）中刺激的全部或部分所述胚状体和支架材料的缀合物的步骤，（c）将所述步骤（b）中制备的所述缀合物移植到 （d）在所述动物中制造源自所述缀合物的全层皮肤的步骤。

公开(公告)号：EP3190176A1

公开(公告)日：2017-07-12

申请号：EP16150783.5

申请日：2016-01-11

申请人： IMBA-Institut für Molekulare Biotechnologie GmbH

发明人： The designation of the inventor has not yet been filed

IPC分类号： C12N5/071 ,  C12N5/0735 ,  C12N5/074 ,  C12N5/0793

CPC分类号： C12N5/0696 ,  C12N5/0606 ,  C12N5/0619 ,  C12N5/0697 ,  C12N2501/115 ,  C12N2501/33 ,  C12N2501/385 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2513/00 ,  C12N2527/00 ,  C12N2531/00 ,  C12N2533/40 ,  C12N2533/52 ,  C12N2533/90 ,  C12N2535/10

摘要： The present invention provides an elongated or fiber-supported multicellular aggregation of multipotent cells, wherein multipotent cells are arranged in an oblong or longish arrangement with an aspect ratio of a prolate dimension to a perpendicular dimension of at least 2:1, or supported by a fibrous structure, and wherein said aggregate contains cells at different stages of differentiation, and said aggregate contains polar cells; methods of generating such aggregates; methods of developing the aggregates further into tissue organoids and kits for such methods.

摘要翻译： 本发明提供细长或纤维支撑的多能细胞的多细胞聚集体，其中多能细胞排列成椭圆形或长方形排列，其长宽比与长径比至少为2:1或由 纤维结构，并且其中所述聚集体含有处于不同分化阶段的细胞，并且所述聚集体含有极性细胞; 产生这种聚集体的方法; 用于将这些聚集体进一步发展成组织器官和用于这些方法的试剂盒的方法。

公开(公告)号：EP3183336A2

公开(公告)日：2017-06-28

申请号：EP15756227.3

申请日：2015-08-21

申请人： Cambridge Enterprise Limited

发明人： SMITH, Austin ,  GUO, Ge ,  TAKASHIMA, Yasuhiro

IPC分类号： C12N5/0735 ,  C12N5/074

CPC分类号： C12N5/0696 ,  C12N5/0606 ,  C12N2500/38 ,  C12N2500/99 ,  C12N2501/065 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2501/235 ,  C12N2501/415 ,  C12N2501/60 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/724 ,  C12N2501/727 ,  C12N2501/73 ,  C12N2501/90 ,  C12N2501/999 ,  C12N2510/00 ,  C12N2533/52

摘要： The invention provides methods and materials for resetting or sustaining a human stem cell in a “naïve” or “ground” state, based on the use of media including combinations of inhibitors. An example naïve culture medium comprises a PKC inhibitor, a MEK inhibitor. Also provided are methods of obtaining or propagating such cells, cells obtained using these methods, and novel culture media, which can be used in these methods.

摘要翻译： 基于使用包括抑制剂组合的介质，本发明提供了用于重置或维持处于“幼稚”或“磨碎”状态的人类干细胞的方法和材料。 未经培养的培养基的例子包括PKC抑制剂，MEK抑制剂。 还提供了获得或繁殖这些细胞的方法，使用这些方法获得的细胞以及可用于这些方法的新型培养基。

公开(公告)号：EP3071040A4

公开(公告)日：2017-06-21

申请号：EP14858383

申请日：2014-10-30

申请人： THE CURATORS OF THE UNIV OF MISSOURI ,  MEMPHIS MEATS INC

发明人： GENOVESE NICHOLAS J ,  ROBERTS R MICHAEL ,  TELUGU BHANU PRAKASH V L

IPC分类号： A01N63/00 ,  A01N65/00 ,  A23L13/00 ,  A61K35/28 ,  A61K35/35 ,  A61K35/545 ,  C12N5/077 ,  C12N5/10

CPC分类号： C12N5/0658 ,  A23L13/00 ,  A23V2002/00 ,  A61K35/28 ,  A61K35/545 ,  C12N5/10 ,  C12N2501/392 ,  C12N2501/40 ,  C12N2501/415 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/72 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45

摘要： The present disclosure relates to methods for enhancing cultured meat production, such as livestock-autonomous meat production. In certain aspects, the meat is any metazoan tissue or cell-derived comestible product intended for use as a comestible food or nutritional component by humans, companion animals, domesticated or captive animals whose carcasses are intended for comestible use, service animals, conserved animal species, animals used for experimental purposes, or cell cultures.

摘要翻译： 本公开涉及用于增强培养肉类生产的方法，例如家畜自主肉类生产。 在某些方面，肉是任何后生动物组织或细胞衍生的可食用产品，其打算用作人类，伴侣动物，其胴体旨在用于食用的驯化或捕获动物，服务动物，保存动物物种 ，用于实验目的的动物或细胞培养物。

公开(公告)号：EP2794860B1

公开(公告)日：2017-05-03

申请号：EP12860444

申请日：2012-12-19

申请人： UNIV KYOTO

发明人： OSAFUNE KENJI ,  ARAOKA TOSHIKAZU

IPC分类号： C12N5/10 ,  C12N5/073 ,  C12N5/0735 ,  C12N5/0775 ,  C12N15/09

CPC分类号： C12N5/0605 ,  C12N5/0686 ,  C12N2501/155 ,  C12N2501/385 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/45

摘要： The present invention provides a method for inducing differentiation of a human pluripotent stem cell into an intermediate mesoderm cell without the use of a growth factor. Such method comprises performing culture in a medium containing a specific compound, so as to induce differentiation of a human pluripotent stem cell into an intermediate mesoderm cell.

摘要翻译： 本发明提供了一种用于在不使用生长因子的情况下诱导人多能干细胞分化成中间胚层细胞的方法。 这种方法包括在含有特定化合物的培养基中进行培养，以诱导人类多能干细胞分化成中间胚层细胞。

公开(公告)号：EP3149156A2

公开(公告)日：2017-04-05

申请号：EP15728704.6

申请日：2015-05-27

申请人： Children's Hospital Medical Center

发明人： WELLS, James, Macormack ,  MCCRACKEN, Kyle, William

IPC分类号： C12N5/071

CPC分类号： C12N5/0697 ,  C12N5/0679 ,  C12N2501/11 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2506/45

摘要： Disclosed are methods of inducing formation of a gastric cells and/or a gastric tissue, such as in the form of a gastric organoid. The formation of gastric cells and/or tissue may be carried out by the activating and/or inhibiting of one or more signaling pathways within a precursor cell. Also disclosed are methods for using the disclosed gastric cells, gastric tissues, and/or gastric organoids derived from precursor cells.

摘要翻译： 公开了诱导形成胃细胞和/或胃组织，：例如在胃组织体的形式的方法。 胃细胞和/或组织的形成可以由小区内的前体的激活和一种或多种信号传导途径/或抑制来进行。 所以圆盘游离缺失是使用盘游离缺失胃细胞，胃组织，和/或胃类器官由前体细胞的方法。