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    COUNTING PARTICLES USING AN ELECTRICAL DIFFERENTIAL COUNTER
    24.
    发明公开
    COUNTING PARTICLES USING AN ELECTRICAL DIFFERENTIAL COUNTER 审中-公开
    ZÄHLUNGVON TEILCHEN MITHILEL EINES ELEKTRISCHEN差异化

    公开(公告)号：EP2638393A4

    公开(公告)日：2016-06-01

    申请号：EP11839117

    申请日：2011-11-09

    申请人： GEN HOSPITAL CORP ,  MASSACHUSETTS INST TECHNOLOGY ,  UNIV ILLINOIS ,  DAKTARI DIAGNOSTICS INC

    发明人： WATKINS NICHOLAS ,  BASHIR RASHID ,  RODRIGUEZ WILLIAM ,  CHENG XUANHONG ,  TONER MEHMET ,  CHEN GRACE ,  OPPENHEIMER AARON

    IPC分类号： G01N33/53 ,  C12M1/34 ,  G01N15/10 ,  G01N15/12 ,  G01N15/14 ,  G01N21/17 ,  G01N33/52 ,  G01N33/543 ,  G01N33/569

    CPC分类号： G01N27/02 ,  B01L2200/0652 ,  B01L2300/0877 ,  C12M41/36 ,  G01N15/12 ,  G01N15/1245 ,  G01N33/56972 ,  G01N2015/1006 ,  G01N2015/1486

    摘要： This disclosure relates to methods and devices to count particles of interest, such as cells. The methods include obtaining a fluid sample that may contain particles of interest; counting all types of particles in a portion of the sample using a first electrical differential counter to generate a first total; removing any particles of interest from the portion of the fluid sample; counting any particles remaining in the portion of the fluid sample using a second electrical differential counter after the particles of interest are removed to generate a second total; and calculating a number of particles of interest originally in the fluid sample by subtracting the second total from the first total, wherein the difference is the number of particles of interest in the sample. These methods and related devices can be used, for example, to produce a robust, inexpensive diagnostic kit for CD4+ T cell counting in whole blood samples.

    摘要翻译： 本公开涉及计算感兴趣的颗粒，例如细胞的方法和装置。 所述方法包括获得可含有感兴趣的颗粒的流体样品; 使用第一电差分计数器对样品的一部分中的所有类型的颗粒计数以产生第一总数; 从所述流体样品的所述部分去除任何感兴趣的颗粒; 在除去感兴趣的颗粒之后，使用第二电差速计数器对流体样品部分中残留的任何颗粒进行计数以产生第二总数; 以及通过从所述第一总数中减去所述第二总计来计算最初在所述流体样本中的感兴趣的粒子数，其中所述差是所述样本中所关注的粒子的数量。 这些方法和相关装置可用于例如在全血样品中产生用于CD4 + T细胞计数的稳健且廉价的诊断试剂盒。

    Method and apparatus for the discrimation of the cell load in milk
    28.
    发明公开
    Method and apparatus for the discrimation of the cell load in milk 有权
    Verfahren und Vorrichtung zur Unterscheidung der Zelllast in Milch

    公开(公告)号：EP2916131A1

    公开(公告)日：2015-09-09

    申请号：EP14157928.4

    申请日：2014-03-05

    申请人： Amphasys AG

    发明人： Di Berardino, Marco ,  Schade-Kampmann, Grit

    IPC分类号： G01N33/04 ,  A01J5/013 ,  G01N15/12

    CPC分类号： G01N33/48735 ,  A01J5/0132 ,  A01J5/0133 ,  G01N15/1031 ,  G01N15/1056 ,  G01N33/04 ,  G01N33/48785

    摘要： The present invention refers to a method for the discrimination of cells from other particles, as well as of different types of cells in raw milk samples by impedance microflow cytometry. More precisely, it describes a method of analysing the quality of raw milk in terms of its bacterial and somatic cell content without the need to pre-treat the milk sample, such that the analysis can take place directly at the production site. One advantage of the invention is discriminating and counting somatic cells from milk particles (consisting mainly of lipid vesicles) by highfrequency impedance analysis directly performed on untreated raw milk. Another advantage of this invention is that the method allows diagnosing the status of a mastitis infection directly after milking according to the analysis of the somatic cell count. Another advantage of this invention is to allow a fast method for determining the bacterial cell count in raw milk directly after milking. In addition, viability of both somatic and bacterial cells can be determined without the need of any cell label. A further advantage is, that the analysis can be obtained in realtime, directly after the raw milk has passed the micro channel of the microfluidic device.

    摘要翻译： 本发明涉及通过阻抗微流式细胞术鉴别生奶样品中其它颗粒以及不同类型细胞的细胞的方法。 更准确地说，它描述了一种以细菌和体细胞含量为基础来分析生奶的质量的方法，而不需要预处理牛奶样品，使得分析可以直接在生产现场进行。 本发明的一个优点是通过直接在未经处理的生牛奶上进行的高频阻抗分析来区分并计数来自乳颗粒（主要由脂质囊泡组成）的体细胞。 本发明的另一个优点是该方法允许根据体细胞计数的分析直接在挤奶之后诊断乳腺炎感染的状态。 本发明的另一个优点是允许在挤奶后直接测定生奶中的细菌细胞计数的快速方法。 此外，可以确定体细胞和细菌细胞的活力，而不需要任何细胞标记。 另一个优点是，可以在生奶通过微流体装置的微通道之后直接获得实时分析。