公开(公告)号：EP1389997B1

公开(公告)日：2010-12-08

申请号：EP02725654.4

申请日：2002-04-12

申请人： BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH

发明人： KIRK, Dwayne ,  MASON, Hugh ,  WALMSLEY, Amanda ,  ARNTZEN, Charles

IPC分类号： C12N15/82 ,  C12N15/36 ,  A01H5/00

CPC分类号： C12N15/8258 ,  C07K14/245 ,  C07K14/705 ,  C12N15/8257

摘要： The present invention provides for processing methods that preserve pharmaceutical proteins expressed in plants. Herein raw plant tissue is reduced to a stable homogenate without significant loss of protein or pharmaceutical potency. The homogenate can be used directly for pharmaceutical purposes without the need to further extract, purify, or precipitate the pharmaceutical protein. The present invention further provides a method of effective immunocontraception for animal and human application. Methods are disclosed for producing transgenic plant or plant cells which express contraceptive proteins, and which can be delivered whole, in part, or after processing, to an animal to cause a contraceptive effect in the target species.

公开(公告)号：EP0638124B1

公开(公告)日：2003-06-18

申请号：EP93910813.0

申请日：1993-04-26

申请人： BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH, INC.

发明人： WOOD, H., Alan

IPC分类号： C12N15/86 ,  C12N15/34 ,  A01K67/033 ,  A01N63/00 ,  C12N7/02

CPC分类号： C12N7/00 ,  A01K67/033 ,  A01N63/00 ,  A61K38/00 ,  A61K39/00 ,  C07K14/005 ,  C12N15/86 ,  C12N2710/14122 ,  C12N2710/14143 ,  C12N2710/14151

摘要： A method of infecting insects is disclosed; the method utilizes a form of a baculovirus which is highly efficient at establishing infection and is normally destined to become occluded within the polyhedrin or granulin - Pre-occluded Virus (POV). Specifically, the POV as derived from a polyhedrin-minus or granulin-minus (lacking a functional polyhedrin or granulin gene) baculovirus is fed to insect larvae per os resulting in high infection rates. The discovery of the POV form of polyhedrin-minus baculoviruses was essential to our invention of the novel method of infecting insect larvae per os using the POV form of polyhedrin-minus baculovirus, and as such is also disclosed.

公开(公告)号：EP1093821A3

公开(公告)日：2003-01-02

申请号：EP00121917.9

申请日：2000-10-07

申请人： HEALTH RESEARCH, INC. ,  BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH, INC.

发明人： Thanavala, Yasmin ,  Arntzen, Charles J.

IPC分类号： A61K39/29

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K39/292 ,  A61K2039/5156 ,  A61K2039/542 ,  A61K2039/545 ,  A61K2039/555 ,  A61K2039/55505 ,  A61K2039/55544 ,  A61K2039/57 ,  C12N15/8258 ,  C12N2730/10122 ,  C12N2730/10134 ,  Y02A50/386 ,  Y02A50/388 ,  Y02A50/40 ,  Y02A50/403 ,  Y02A50/407 ,  Y02A50/472

摘要： A method for obtaining an immune response to a non-enteric pathogen antigen (NEPA) such as hepatitis B surface antigen (HBsAg) by feeding the antigen in a plant material to an animal that is immunoreceptive to the NEPA. It has now been discovered that the animal may be made immunoreceptive to the NEPA such as HBsAg by administering the plant material containing the NEPA in conjunction with a suitable adjuvant. The plant material is a substance comprising a physiologically acceptable plant material, especially potatoes, containing the NEPA, e.g. hepatitis B surface antigen (HBsAg). The NEPA, e.g. HBsAg in the plant results from expression by the plant of the NEPA due to genetic alteration.

公开(公告)号：EP1175144A2

公开(公告)日：2002-01-30

申请号：EP99967556.4

申请日：1999-12-22

申请人： BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH ,  Mason, Hugh S. ,  Arntzen, Charles J.

发明人： MASON, Hugh, S. ,  ARNTZEN, Charles, J.

IPC分类号： A01H1/00 ,  A01H1/06 ,  A61K39/106 ,  A61K39/108 ,  C07H21/04 ,  C07K14/28 ,  C07K14/415 ,  C12N5/14 ,  C12N15/00

CPC分类号： C07K14/245 ,  A61K39/00 ,  A61K2039/515 ,  A61K2039/542 ,  C07K14/28 ,  C12N15/8242 ,  C12N15/8257 ,  Y02A50/472

摘要： The invention provides mutant Escherichia coli heat labile (LT) and Vibrio cholerae toxin (CT) polypeptides and the polynucleotides that encode them. The mutant LT and CT polypeptides can be readily produced in plants and can be used to treat or prevent diseases caused by E. coli and V. cholera. The polypeptides are also useful as adjuvants.

公开(公告)号：EP0676918B1

公开(公告)日：1997-07-30

申请号：EP94903541.4

申请日：1993-12-09

申请人： BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH, INC.

发明人： HUGHES, Patrick, R.

IPC分类号： A01K67/033

CPC分类号： A01K67/033

摘要： Method and apparatus for rearing insects is disclosed. Information concerning the physical and dietary needs of the insect as well as behavioral characteristics are utilized to maximize the number of larvae reared per unit surface area of diet and per unit of rearing area while minimizing the amount of labor and materials required. An enclosed rearing unit (1) is provided which can be located within an appropriate environment for rearing the insects. There are three sections within the rearing unit: 1) a diet space (2), 2) a larval space (3), and 3) a frass space (4). The diet space (2) includes an appropriate diet medium for the insects. The larval space (3) is located below the diet space (2) and includes a series of vertical partitions (12) perpendicular to and in contact with or nearly in contact with the diet medium such that the insect larvae are able to disperse themselves over the partitions (12). The frass space (4) is located below the larval space (3) such that any frass collects within the frass space (4) as it is produced and does not interfere with the larval space (3) or the diet space (2). The rearing system can further include an emergence pan (30) including an outlet for allowing emerging adults to enter an oviposition cage. The emergence pan (30) replaces the frass collection pan (24) when all of the larvae have pupated. The rearing unit is turned upside down such that the emergence pan (30) is located above the larval space (3) and the diet space (2).

公开(公告)号：EP0676918A1

公开(公告)日：1995-10-18

申请号：EP94903541.0

申请日：1993-12-09

申请人： BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH, INC.

发明人： HUGHES, Patrick, R.

IPC分类号： A01K67

CPC分类号： A01K67/033

摘要： Method and apparatus for rearing insects is disclosed. Information concerning the physical and dietary needs of the insect as well as behavioral characteristics are utilized to maximize the number of larvae reared per unit surface area of diet and per unit of rearing area while minimizing the amount of labor and materials required. An enclosed rearing unit (1) is provided which can be located within an appropriate environment for rearing the insects. There are three sections within the rearing unit: 1) a diet space (2), 2) a larval space (3), and 3) a frass space (4). The diet space (2) includes an appropriate diet medium for the insects. The larval space (3) is located below the diet space (2) and includes a series of vertical partitions (12) perpendicular to and in contact with or nearly in contact with the diet medium such that the insect larvae are able to disperse themselves over the partitions (12). The frass space (4) is located below the larval space (3) such that any frass collects within the frass space (4) as it is produced and does not interfere with the larval space (3) or the diet space (2). The rearing system can further include an emergence pan (30) including an outlet for allowing emerging adults to enter an oviposition cage. The emergence pan (30) replaces the frass collection pan (24) when all of the larvae have pupated. The rearing unit is turned upside down such that the emergence pan (30) is located above the larval space (3) and the diet space (2).

公开(公告)号：EP0384294A3

公开(公告)日：1991-06-05

申请号：EP90102939.7

申请日：1990-02-15

申请人： BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH, INC.

发明人： Granados, Robert R. ,  Hashimoto, Yoshifumi, Dept. of Applied Biology

IPC分类号： C12N15/34 ,  A01N63/00 ,  C07K15/04

CPC分类号： C12N15/8286 ,  A01N63/02 ,  C07K14/005 ,  C12N2710/14122 ,  Y02A40/162

摘要： A gene coded for a polypeptide isolated from an occlusion body of a baculovirus such as Trichoplusia ni granulosis virus possessing a biological activity of enhancing virus infection of host insects by causing rapid degradation of the peritrophic membrane lining the midgut lumen of insects. Novel pesticides incorporating said gene or fragments thereof are also claimed.

摘要翻译： 编码用于分离自杆状病毒（例如粉纹夜蛾）病毒的杆状病毒的闭塞体的多肽的基因，具有通过引起昆虫中肠内脏的内皮细胞膜的快速降解来增强宿主昆虫的病毒感染的生物学活性。 还要求引用所述基因或其片段的新型农药。

公开(公告)号：EP0341611A2

公开(公告)日：1989-11-15

申请号：EP89108173.9

申请日：1989-05-05

申请人： BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH, INC. ,  CORNELL RESEARCH FOUNDATION, INC.

发明人： Wood, Harry A. ,  Parrish, Colin R.

IPC分类号： A61K39/23 ,  C12N15/00

CPC分类号： C12N15/86 ,  A61K39/00 ,  C07K14/005 ,  C12N2710/14143 ,  C12N2750/14322 ,  Y10S424/818

摘要： A recombinant subunit vaccine for protection dogs against infection caused by canine parvovirus comprising VP-2 protein produced during replication of a recombinant baculovirus in insect tissue culture cells or insects which are a permissive hosts for the replication of selected baculoviruses.

摘要翻译： 一种用于保护狗免受犬细小病毒引起的感染的重组亚单位疫苗，包括在昆虫组织培养细胞中复制重组杆状病毒期间产生的VP-2蛋白或作为复制选择的杆状病毒的允许宿主的昆虫。

公开(公告)号：EP0274527A1

公开(公告)日：1988-07-20

申请号：EP87905086.0

申请日：1987-07-21

申请人： BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH, INC. ,  TEXAS A & M UNIVERSITY SYSTEM

发明人： LEGOCKI, Roman, P. ,  LEGOCKI, Misuk ,  SZALAY, Aladar, A. ,  BALDWIN, Thomas, O.

IPC分类号： C12N9 ,  C12N15 ,  C12Q1

CPC分类号： C12N9/0071 ,  C12N15/90 ,  C12Q1/06 ,  C12Q1/6897 ,  C12Y114/14003

摘要： Un micro-organisme hôte est modifié génétiquement de façon stable par insertion, dans l'un ou l'autre de ses emplacements chromosomiques non essentiels, d'un fragment d'ADN étranger comprenant un fragment non homologue contenant un gène lux AB d'une bactérie bioluminescente sélectionnée, telle que V. harveyi, de telle sorte que le fragment non homologue est reproduit de façon stable en tant qu'une partie du chromosome des cellules du micro-organisme hôte au fur et à mesure qu'elles se divisent dans un millieu de croissance, l'expression génétique desdits micro-organismes déclenchant la production de luciférase, qui à son tour émet de la lumière en présence d'aldéhyde servant ainsi de mesure de l'expression génétique. La bactérie Gram-positive et la bactérie Gram-négative sont toutes les deux des micro-organismes qui peuvent être modifiés de la façon décrite ci-dessus. Un radiofilm peut être employé pour mesurer la quantité de lumière émise depuis un micro-organisme grâce à l'utilisation de plusieurs gouttelettes contenant ledit micro-organisme et présentant un nombre de cellules (ou de plasmides) connu et approprié.