公开(公告)号：EP3147369A4

公开(公告)日：2018-02-07

申请号：EP15796845

申请日：2015-05-21

申请人： AJINOMOTO KK ,  SPIBER INC

发明人： NAKASE KENTARO

IPC分类号： C12P21/02 ,  C12N1/00 ,  C12N1/21 ,  C12N15/09

CPC分类号： C07K14/43518 ,  C12N1/00 ,  C12N15/09 ,  C12N15/70 ,  C12P21/02

摘要： A method for producing a fibroin-like protein is provided. A fibroin-like protein is produced by a method for producing a fibroin-like protein comprising culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein cell proliferation after inducing the expression is reduced.

公开(公告)号：EP3147369A1

公开(公告)日：2017-03-29

申请号：EP15796845.4

申请日：2015-05-21

申请人： Ajinomoto Co., Inc. ,  Spiber Inc.

发明人： NAKASE, Kentaro

IPC分类号： C12P21/02 ,  C12N1/00 ,  C12N1/21 ,  C12N15/09

CPC分类号： C07K14/43518 ,  C12N1/00 ,  C12N15/09 ,  C12N15/70 ,  C12P21/02

摘要： A method for producing a fibroin-like protein is provided. A fibroin-like protein is produced by a method for producing a fibroin-like protein comprising culturing Escherichia coli having a gene encoding the fibroin-like protein in a medium, inducing expression of the gene encoding the fibroin-like protein, and collecting the fibroin-like protein, wherein cell proliferation after inducing the expression is reduced.

摘要翻译： 提供丝心蛋白样蛋白质的制造方法。 通过生产丝心蛋白样蛋白质的方法制备丝心蛋白样蛋白质，包括在培养基中培养具有编码丝心蛋白样蛋白质的基因的大肠杆菌，诱导编码丝心蛋白样蛋白质的基因的表达，并收集丝心蛋白 样蛋白，其中诱导表达后的细胞增殖减少。

公开(公告)号：EP2990414A1

公开(公告)日：2016-03-02

申请号：EP14787823.5

申请日：2014-04-18

申请人： Spiber Inc.

发明人： OSAWA, Toshiaki ,  MORITA, Keisuke

IPC分类号： C07K14/435 ,  A61K8/64

CPC分类号： C07K14/43518 ,  A61K8/0279 ,  A61K8/42 ,  A61K8/46 ,  A61K8/64 ,  A61K9/5169 ,  A61K47/42 ,  A61K2800/412 ,  A61K2800/56 ,  A61K2800/84 ,  A61Q19/00 ,  C08J3/05 ,  C08J3/07 ,  C08J3/12 ,  C08J2389/00 ,  C08L89/00 ,  C09D7/65 ,  C09D7/68

摘要： Polypeptide particles of the present invention are particles of a polypeptide derived from spider silk proteins, and have an average particle size of 1000 nm or less. A method for producing polypeptide particles of the present invention includes: a solution production step in which the polypeptide is dissolved in at least one solvent selected from the group consisting of DMSO, DMF, and these with an inorganic salt, so as to obtain a solution of the polypeptide; a step in which the solution produced in the solution production step is substituted with a water-soluble solvent so as to obtain an aqueous solution of the polypeptide; and a step in which the aqueous solution of the polypeptide is dried. Thereby, the present invention provides polypeptide particles suitable for application to a living body and capable of being applied to cosmetics, etc., while identifying the properties of the polypeptide particles, and a method for producing the same.

摘要翻译： 本发明的多肽颗粒是来源于蜘蛛丝蛋白质的多肽颗粒，其平均粒径为1000nm以下。 本发明的多肽粒子的制造方法包括：溶液制备工序，将多肽溶解在选自DMSO，DMF中的至少一种溶剂中，用无机盐溶解，得到溶液 的多肽; 将溶液制备步骤中产生的溶液用水溶性溶剂置换以获得多肽水溶液的步骤; 以及干燥多肽水溶液的步骤。 由此，本发明提供了适用于生物体的多肽颗粒及其制造方法，同时鉴定多肽颗粒的性质，并且能够应用于化妆品等。

公开(公告)号：EP2990413A1

公开(公告)日：2016-03-02

申请号：EP14787816.9

申请日：2014-04-18

申请人： Spiber Inc.

发明人： OSAWA, Toshiaki ,  MORITA, Keisuke

IPC分类号： C07K14/435 ,  A61K47/42 ,  C07K1/02 ,  C08J9/28 ,  A61K8/64 ,  C08L89/00

CPC分类号： C07K14/43518 ,  A61K8/0279 ,  A61K8/42 ,  A61K8/46 ,  A61K8/64 ,  A61K8/987 ,  A61K9/19 ,  A61K47/42 ,  A61K2800/10 ,  A61K2800/56 ,  A61K2800/84 ,  A61Q19/00 ,  B01J20/24 ,  C08J9/28 ,  C08J2201/0484 ,  C08J2201/0544 ,  C08J2205/022 ,  C08J2205/028 ,  C08J2389/00 ,  C08L89/00 ,  C11B9/00

摘要： A polypeptide porous body of the present invention is a porous body of a polypeptide derived from spider silk proteins. The polypeptide includes a water-insoluble polypeptide. The polypeptide porous body has an apparent density of 0.1 g/cm 3 or less. A method for producing the polypeptide porous body includes: a solution production step in which the polypeptide is dissolved in at least one solvent selected from DMSO, DMF, and these with an inorganic salt, so as to obtain a solution of the polypeptide; a step in which the solution produced in the solution production step is substituted with a water-soluble solvent so as to obtain a polypeptide gel; and a step in which the polypeptide gel is dried. Thereby, the present invention provides a polypeptide porous body having excellent water absorbability and a polypeptide porous body suitable for application to a living body, and a method for producing the same.

摘要翻译： 本发明的多肽多孔体是衍生自蜘蛛丝蛋白的多肽的多孔体。 多肽包括水不溶性多肽。 多孔多孔体的表观密度为0.1g / cm 3以下。 制备多孔多孔体的方法包括：溶液制备步骤，其中将多肽溶解于至少一种选自DMSO，DMF的溶剂中，并用无机盐溶解，得到多肽的溶液; 在溶液制备工序中产生的溶液用水溶性溶剂代替以获得多肽凝胶的步骤; 和多肽凝胶干燥的步骤。 因此，本发明提供了具有优异的吸水性的多孔多孔体和适用于生物体的多孔多孔体及其制造方法。

公开(公告)号：EP2940066A1

公开(公告)日：2015-11-04

申请号：EP13867491.6

申请日：2013-12-17

申请人： Spiber Inc.

发明人： SEKIYAMA Kaori ,  ISHIKAWA Mizuki ,  MURATA Shinya

IPC分类号： C08J5/18 ,  C08L89/00 ,  C07K14/435

摘要： A film of the present invention contains a polypeptide derived from spider silk proteins. The decomposition temperature of the film is 240 to 260°C. The film absorbs ultraviolet light having a wavelength of 200 to 300 nm and has a light transmittance of 85% or more at a wavelength of 400 to 780 nm. The film is transparent and colorless in a visible light region. A method for producing a film of the present invention includes: dissolving a polypeptide derived from spider silk proteins in a dimethyl sulfoxide solvent to prepare a dope; and cast-molding the dope on a surface of a base. Thus, the present invention provides a spider silk protein film that can be formed easily and has favorable stretchability, and a method for producing the same.

摘要翻译： 本发明的膜包含衍生自蜘蛛丝蛋白的多肽。 膜的分解温度为240〜260℃。 该膜吸收波长为200〜300nm的紫外线，并且在400〜780nm的波长下具有85％以上的透光率。 该膜在可见光区域是透明和无色的。 本发明的膜的制造方法包括：将来自蜘蛛丝蛋白的多肽溶解在二甲基亚砜溶剂中以制备涂料; 并将涂料浇铸在基材的表面上。 因此，本发明提供能够容易地形成并具有良好的拉伸性的蜘蛛丝蛋白质膜及其制造方法。

公开(公告)号：EP2397543A1

公开(公告)日：2011-12-21

申请号：EP09839182.4

申请日：2009-01-29

申请人： Spiber Inc.

发明人： SUGAHARA, Junichi ,  SEKIYAMA, Kazuhide

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12Q1/68

CPC分类号： C12N15/1065 ,  G06F19/22

摘要： Provided is a method of determining a DNA tag which is a base sequence to be introduced into a genomic DNA sequence of an organism and an introduction site of the DNA tag into the genomic DNA sequence. The method includes : a step (S1) of obtaining a protein coding sequence of the genomic DNA sequence; a step (S2) of determining a region to be treated in the protein coding sequence; a step (S3) of fragmenting the coding sequence in the region to be treated; a step (S4) of subjecting the fragmented coding sequences to a silent mutation; a step (S5) of determining sequences suitable as primers from polynucleotides including the fragmented coding sequences or complementary base sequences thereof; a step (S6) of performing a homology search for the coding sequences thus determined; and a step (S8) of determining the determined coding sequence corresponding to a minimum value of an NMS as the DNA tag, and determining the site of the fragmented coding sequence corresponding to the determined coding sequence in the protein coding sequence as the introduction site.

摘要翻译： 本发明提供一种测定作为将生物体的基因组DNA序列引入碱基序列的DNA标签的方法和DNA标签的引入位点到基因组DNA序列中的方法。 该方法包括：获得基因组DNA序列的蛋白质编码序列的步骤（S1） 确定蛋白质编码序列中待处理区域的步骤（S2）; 将待处理的区域中的编码序列分段的步骤（S3） 使片段编码序列进行沉默突变的步骤（S4） 确定适合作为引物的序列的步骤（S5），所述多核苷酸包括片段编码序列或其互补碱基序列; 对如此确定的编码序列执行同源性搜索的步骤（S6）; 以及步骤（S8），确定对应于作为DNA标签的NMS的最小值的确定的编码序列，并确定与蛋白质编码序列中所确定的编码序列相对应的片段编码序列的位点作为引入位点。

公开(公告)号：EP4410822A1

公开(公告)日：2024-08-07

申请号：EP22889984.5

申请日：2022-11-02

申请人： Spiber Inc.

发明人： SAKATA Kazuki ,  SHIRAKAWA Seiji ,  KITAHARA Nao ,  NIWA Takahiro

IPC分类号： C07K14/00 ,  C07K1/02 ,  C08G69/48

CPC分类号： C07K14/00 ,  C08G69/48 ,  C07K1/02

摘要： The present invention provides a β-ketoester of a protein containing an amino acid having a hydroxyl group, wherein the hydroxyl group forms an ester bond with a group represented by Formula (1) wherein R represents a C1-6 alkyl group or an optionally substituted aryl group.

公开(公告)号：EP4159862A1

公开(公告)日：2023-04-05

申请号：EP21812733.0

申请日：2021-05-25

申请人： Spiber Inc.

发明人： LIPS David ,  VERKLEIJ Gijs

IPC分类号： C12N15/62 ,  C12N5/10 ,  C12N15/76 ,  C40B40/06

摘要： The present invention relates to a method of preparing a gene cluster construct including a plurality of genes encoding a multi-modular biosynthetic enzyme, the method including (A) a step of preparing a plurality of DNA fragments which are capable of reconstructing the gene cluster construct and have structures that can be ligated to each other and (B) a step of ligating the plurality of DNA fragments prepared in the step (A) to each other by mixing the plurality of DNA fragments in a solution to obtain the gene cluster construct.

公开(公告)号：EP4122948A1

公开(公告)日：2023-01-25

申请号：EP21772323.8

申请日：2021-03-16

申请人： Spiber Inc.

发明人： SAKATA, Kazuki ,  KITAHARA, Nao ,  SHIRAKAWA, Seiji ,  TAKAHASHI, Kentaro ,  MUTO, Leona ,  SHAFAAT, Oliver Syed ,  KAGATA, Hideki

IPC分类号： C07K14/435 ,  C08G81/00 ,  C08H1/00 ,  C08L89/00 ,  C08L101/16 ,  C12N15/12 ,  C12P21/02

摘要： An aspect of the present disclosure provides a synthetic polymer containing a first segment containing a polypeptide skeleton and one or a plurality of second segments bonded directly to the first segment, in which the second segment contains a molecular group having a plasticizing function for the polypeptide skeleton.

公开(公告)号：EP4098127A1

公开(公告)日：2022-12-07

申请号：EP21747234.9

申请日：2021-01-29

申请人： Spiber Inc.

发明人： CHUANG Yu Chun ,  JIANG Li ,  NAIDU Arin

IPC分类号： A23J3/00 ,  A23J3/14 ,  A23J3/22 ,  C07K14/00 ,  C07K14/415 ,  C07K14/435 ,  A23L13/00

摘要： To provide a meat substitute composition having a texture and sensory properties closer to those of real meat than a meat substitute composition in the related art, the present disclosure provides meat substitute composition containing a polypeptide that satisfies the following (1) or (2):
(1) number of amino acid residues is 150 or more, an alanine residue content is 12 to 40%, and a glycine residue content is 11 to 55%; or
(2) an amino acid residue content of at least one type selected from the group consisting of serine, threonine, and tyrosine, an alanine residue content, and a glycine residue content account for 56% or more altogether.