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    Method for producing L-isoleucine by fermentation
    32.
    发明公开
    Method for producing L-isoleucine by fermentation 失效
    通过发酵生产L-精油的方法

    公开(公告)号：EP0071023A3

    公开(公告)日：1984-03-07

    申请号：EP82105666

    申请日：1982-06-25

    申请人： AJINOMOTO CO., INC.

    发明人： Tsuchida, Takayasu ,  Miwa, Kiyoshi ,  Nakamori, Shigeru

    IPC分类号： C12N15/00 ,  C12P13/06 ,  C12R01/13 ,  C12R01/15

    CPC分类号： C12P13/06 ,  C12N15/52 ,  C12N15/77

    摘要： L-isoleucine is produced by a fermentation process comprising aerobically culturing in an aqueous culture medium an L-isoleucine producing microorganism obtained by isolating a transformed strain resistant to α-amino-β-hydroxy valeric acid prepared by incorporating into a recipient strain of the genus Brevibacterium or Corynebacterium, which is sensitive to α-amino-β-hydroxy valeric acid, a plasmid DNA obtained from a microorganism of the genus Brevibacterium or Corynebacterium into which has been inserted a fragment of chromosomal DNA derived from a DNA-donor strain of the genus Brevibacterium or Corynebacterium which is resistant to α-amino-β-hydroxy valeric acid; and recovering L-isoleucine which accumulates in the resulting culture liquid.

    Plasmid pHM 1519
    33.
    发明公开
    Plasmid pHM 1519 失效
    质粒pHM 1519。

    公开(公告)号：EP0078537A2

    公开(公告)日：1983-05-11

    申请号：EP82110095.5

    申请日：1982-11-02

    申请人： AJINOMOTO CO., INC.

    发明人： Matsui, Hiroshi ,  Miwa, Kiyoshi ,  Terabe, Mahito ,  Nakamori, Shigeru ,  Sano, Konosuke

    IPC分类号： C12N15/00 ,  C12N1/20

    CPC分类号： C12N15/77

    摘要： An essentially pure plasmid is provided, which is characterized by a molecular weight of 1.8 megadalton and the restriction endonuclease-cleavage chart shown in the Figure, and which is capable of propagating in a Coryneform glutamic acid producing bacterium as a host strain.
    This plasmid may be inserted by a gene which is responsible for the production of a product and the hybrid plasmid may be incorporated in Coryneform glutamic acid producing bacteria as recipient organisms.

    摘要翻译： 提供了基本上纯的质粒，其特征在于分子量为1.8兆体积，并且图中所示的限制性内切核酸酶切割图，其能够在作为宿主菌株的棒状杆菌谷氨酸诱导细菌中繁殖。 该质粒可以通过负责产生产物的基因插入，杂交质粒可以作为受体生物掺入棒状谷氨酸生产菌。