摘要:
A Coryneform bacterium containing a recombinant DNA molecule constructed by operatively connecting a gene coding for shikimate kinase to a plasmid vector capable of replicating in the Coryneform bacterium is disclosed along with methods of preparing this bacterium and of using it in the production of aromatic amino acids.
摘要:
A microorganism which is able to produce one or two or more kinds of L-amino acids selected from the group consisting of L-glutamic acid, Z-glutamine, L-proline, L-ornithine, L-citrulline and L-arginine and is modified to increase α-ketoglutarate synthase activity is cultured in a medium, and the L-amino acids are collected from the medium or the cells.
摘要:
A method for simulating a substance production process using cells based on differential equations concerning intracellular metabolites and gene expression comprising preparing the differential equations concerning intracellular metabolites and gene expression, assigning values for parameters in the differential equations required for the simulation and solving the differential equations with the assigned values, which is characterized by the following (a) to (d): (a) the differential equations include a differential equation including a specific growth rate of the cells, and the specific growth rate is represented as a function of time; (b) all or a part of parameters of the differential equations are represented as a function of the specific growth rate or a function of time; (c) the differential equations include a formation rate for formation of a cell component from an intracellular metabolite, and the formation rate is represented as a function of the specific growth rate or a function of time; and (d) the differential equations include an inflow rate of a metabolite taken up from the outside of the cells and/or an outflow rate of a metabolite excreted out of the cells from the inside of the cells, and the inflow rate and/or the outflow rate is represented as a function of the specific growth rate or a function of time.
摘要:
The present invention relates to a process for producing an aromatic amino acid which comprises culturing a Coryneform bacterium carrying a recombinant DNA constructed by connecting (1) a gene capable of being expressed in a Coryneform bacterial cell and coding for 3-deoxy-D-arabino-heptulonic acid-7-phosphate synthetase with (2) a plasmid vector capable of propagating in a Coryneform bacterial cell.