摘要:
Disclosed herein are a polypeptide having cephalosporin C(CPC) acylase activity and a use thereof. More specifically, a mutant CPC acylase comprising a point mutation introduced thereinto, thereby exhibiting improved enzymatic activity and/or stability, and a use of the mutant CPC acylase for producing 7-aminocephalosporanic acid (7-ACA), are provided.
摘要:
The present invention describes a process for the synthesis of a semi-synthetic β-lactam compound from a nucleus and a side chain selected from the group consisting of D-phenylglycine and D-dihydro-phenylglycine in the form of a side chain ester and an enzyme catalyzing the coupling of the side chain ester to the nucleus characterized in that the side chain ester is not isolated as a solid intermediate.
摘要:
The present invention relates to a cephalosporin-producing microorganism characterized in that it has been transformed with a heterologous CefT gene and whereby the ratio of the cephalosporin production capacity of the microorganism transformed with the heterologous CefT gene compared to the cephalosporin production capacity of the microorganism not being transformed with the heterologous CefT gene is at least 1.2. Furthermore, the present invention provides a method for the production of the cephalosporin-producing microorganism as well as a process for the fermentative production of cephalosporins using the cephalosporin-producing microorganism.
摘要:
Described is a process for the enzymatic production of cephalosporane derivatives, or their salts, having the formula (I):
wherein R represents a -CO-COOH or -COOH group and R' is H, OH or -O-CO-R", R" being a G 1 -C 4 alkyl group, including the oxidative deamination of compounds, or their salts, having the formula (II):
in the presence of a micro-organism of the Rhodotorula type or D-aminoacid oxidase derived therefrom, in free or immobilized form.
摘要:
Transformation of Cephalosporin C or its derivatives and salts into 7-aminocephalosporanic acid or its derivatives by an enzymatic two stage process with enzymes immobilized on a solid matrix.
摘要:
Es wird ein Verfahren zur kontinuierlichen Umsetzung von Cephalosporinderivaten zu den entsprechenden Glutaryl-7-aminocephalosporansäurederivaten in Gegenwart von D-Aminosäure-Oxidase haltigem Katalysator beschrieben. Die Produktausbeute kann gegebenenfalls durch Wasserstoffperoxidzugabe erhöht werden.