公开(公告)号：EP0180961A3

公开(公告)日：1988-01-07

申请号：EP85114070

申请日：1985-11-05

申请人： MEIJI SEIKA KAISHA LTD.

发明人： Makise, Junko ,  Ichikawa, Kazuo ,  Yoshida, Kenji ,  Watanabe, Suzuo

IPC分类号： C07H15/203 ,  C12Q01/34

CPC分类号： C07H15/203 ,  C12Q1/34 ,  C12Q2334/00 ,  G01N2333/924

摘要： A glucosamine derivative is disclosed, represented by formual (I)

wherein R, and R 2 independently represent a halogen atom or a nitro group. A reagent for assaying N-acetyl-β-D-glucosaminidase containing the glucosamine derivative as a substrate can assay a number of specimens with high sensitivity and accuracy in a short period of time.

公开(公告)号：EP0089626A3

公开(公告)日：1985-11-21

申请号：EP83102641

申请日：1983-03-17

申请人： G.D. Searle & Co.

发明人： Brewer, Stephen James ,  Sassenfeld, Helmut Max

IPC分类号： C12N15/00 ,  C12P21/02 ,  G01N33/54 ,  C12Q01/34 ,  C12P21/06

CPC分类号： C12P21/02 ,  C07K14/485 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/50 ,  C07K2319/75 ,  C12Q1/37 ,  Y10S435/803 ,  Y10S930/12

摘要： method for the production of polypeptides utilizing a polymer of charged amino acids and an exopeptidase. A DNA sequence coding for the charged amino acid polymer is inserted adjacent to one end of the polypeptide gene. The polypeptide-charged amino acid polymer fusion product is removed from most contaminents by selecting for the charged amino acid polymer during a first isolation step. Next the charged amino acid polymer is hydrolyzed using an exopeptidase which leaves the polypeptide active. A second isolation step removes the polypeptide from any remaining contaminents. An example using polyarginine and carboxypeptidase B to produce urogastrone is included.

公开(公告)号：EP0075293A3

公开(公告)日：1983-09-14

申请号：EP82108604

申请日：1982-09-17

申请人： AJINOMOTO CO., INC. ,  FUJIREBIO KABUSHIKI KAISHA also trading as FUJIREBIO INC.

发明人： Takenouchi, Tomoharu ,  Kamimura, Akira

IPC分类号： C12N09/99 ,  C12Q01/34 ,  C12N09/38

CPC分类号： C12N9/99 ,  C12Q1/34 ,  G01N2333/924

摘要： β-D-galactosidase catalyzed reactions can be terminated by inhibiting β-D-galalctosidase in a method in which β-D-qalactosidase is brought in contact with a chelating agent at an alkaline pH range other than the optimal pH range for β-D-galactosidase.

公开(公告)号：EP0051985A3

公开(公告)日：1982-08-11

申请号：EP81305261

申请日：1981-11-05

申请人： TECHNICON INSTRUMENTS CORPORATION (a New York corporation)

发明人： Magnusson, Carl-Gustav M. ,  Masson, Pierre L. ,  Collet-Cassart, Daniel

IPC分类号： G01N33/54 ,  C12Q01/34

CPC分类号： G01N33/563 ,  Y10S436/825

摘要： In the immunoassay of a particular protein in a biological fluid, there is frequently interference in the assay by other proteins present in the fluid, e.g. by complement factors or antibodies in human serum.The interference so caused can be avoided by subjecting the fluid to protein-digestion, using for example an enzyme such as pepsin, as a result of which the particular protein of interest, or a fragment thereof, can be assayed without interference by the other proteins. Also, radioallergosorbent tests for particular IgE antibodies can be improved in sensitivity and accuracy, by subjecting the absorbed IgE to enzymic digestion, and then assaying a fragment thereof.

公开(公告)号：EP0034692A3

公开(公告)日：1981-12-23

申请号：EP81100139

申请日：1981-01-10

申请人： BEHRINGWERKE AKTIENGESELLSCHAFT

发明人： Habenstein, Klaus, Dr. ,  Kohl, Helmut, Dr.

IPC分类号： C08B31/12 ,  C08B37/02 ,  C12Q01/34 ,  G01N33/50

CPC分类号： G01N33/52 ,  C08B31/12 ,  C08B37/0021 ,  C12Q1/34 ,  G01N31/221

公开(公告)号：EP0034759A3

公开(公告)日：1981-11-04

申请号：EP81100959

申请日：1981-02-11

申请人： HOECHST AKTIENGESELLSCHAFT

发明人： Blumbach, Jürgen, Dr. ,  Schindler, Peter, Dr.

IPC分类号： C07D501/36 ,  C12Q01/34

CPC分类号： C12Q1/34 ,  C12Q2334/00 ,  G01N2333/986 ,  G01N2415/00